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Accordingly, drugs with novel modes of action are desperately required to combat malaria.\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    parasites infect human red blood cells where they digest the host\u2019s main protein constituent, hemoglobin. Leucine aminopeptidase\n                    <jats:italic>Pf<\/jats:italic>\n                    A-M17 is one of several aminopeptidases that have been implicated in the last step of this digestive pathway. Here, we use both reverse genetics and a compound specifically designed to inhibit the activity of\n                    <jats:italic>Pf<\/jats:italic>\n                    A-M17 to show that\n                    <jats:italic>Pf<\/jats:italic>\n                    A-M17 is essential for\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    survival as it provides parasites with free amino acids for growth, many of which are highly likely to originate from hemoglobin. We further show that loss of\n                    <jats:italic>Pf<\/jats:italic>\n                    A-M17 results in parasites exhibiting multiple digestive vacuoles at the trophozoite stage. In contrast to other hemoglobin-degrading proteases that have overlapping redundant functions, we validate\n                    <jats:italic>Pf<\/jats:italic>\n                    A-M17 as a potential novel drug target.\n                  <\/jats:p>","DOI":"10.7554\/elife.80813","type":"journal-article","created":{"date-parts":[[2022,8,3]],"date-time":"2022-08-03T05:15:13Z","timestamp":1659503713000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.80813","source":"Crossref","is-referenced-by-count":33,"title":["Genetic and chemical validation of Plasmodium falciparum aminopeptidase PfA-M17 as a drug target in the hemoglobin digestion pathway"],"prefix":"10.7554","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5974-8625","authenticated-orcid":true,"given":"Rebecca CS","family":"Edgar","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02czsnj07","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Medicine, Deakin 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