{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T03:05:27Z","timestamp":1791947127036,"version":"4.3.4"},"reference-count":95,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2023,2,6]],"date-time":"2023-02-06T00:00:00Z","timestamp":1675641600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"},{"start":{"date-parts":[[2023,2,6]],"date-time":"2023-02-06T00:00:00Z","timestamp":1675641600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"},{"start":{"date-parts":[[2023,2,6]],"date-time":"2023-02-06T00:00:00Z","timestamp":1675641600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["K99EY030144-01"],"award-info":[{"award-number":["K99EY030144-01"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["1ZIADC000085-01"],"award-info":[{"award-number":["1ZIADC000085-01"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["IRP"],"award-info":[{"award-number":["IRP"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,1,22]]},"abstract":"<jats:p>\n                    During development, retinal progenitors navigate a complex landscape of fate decisions to generate the major cell classes necessary for proper vision. Transcriptional regulation is critical to generate diversity within these major cell classes. Here, we aim to provide the resources and techniques required to identify transcription factors necessary to generate and maintain diversity in photoreceptor subtypes, which are critical for vision. First, we generate a key resource: a high-quality and deep transcriptomic profile of each photoreceptor subtype in adult zebrafish. We make this resource openly accessible, easy to explore, and have integrated it with other currently available photoreceptor transcriptomic datasets. Second, using our transcriptomic profiles, we derive an in-depth map of expression of transcription factors in photoreceptors. Third, we use efficient CRISPR-Cas9 based mutagenesis to screen for null phenotypes in F0 larvae (F0 screening) as a fast, efficient, and versatile technique to assess the involvement of candidate transcription factors in the generation of photoreceptor subtypes. We first show that known phenotypes can be easily replicated using this method: loss of S cones in\n                    <jats:italic>foxq2<\/jats:italic>\n                    mutants and loss of rods in\n                    <jats:italic>nr2e3<\/jats:italic>\n                    mutants. We then identify novel functions for the transcription factor Tbx2, demonstrating that it plays distinct roles in controlling the generation of all photoreceptor subtypes within the retina. Our study provides a roadmap to discover additional factors involved in this process. Additionally, we explore four transcription factors of unknown function (Skor1a, Sall1a, Lrrfip1a, and Xbp1), and find no evidence for their involvement in the generation of photoreceptor subtypes. This dataset and screening method will be a valuable way to explore the genes involved in many other essential aspects of photoreceptor biology.\n                  <\/jats:p>","DOI":"10.7554\/elife.81579","type":"journal-article","created":{"date-parts":[[2023,1,23]],"date-time":"2023-01-23T10:15:13Z","timestamp":1674468913000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.81579","source":"Crossref","is-referenced-by-count":24,"title":["Transcription factors underlying photoreceptor diversity"],"prefix":"10.7554","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9217-3069","authenticated-orcid":true,"given":"Juan M","family":"Angueyra","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}],"name":"Unit of Retinal Neurophysiology, National Eye Institute, National Institutes of Health","place":["Bethesda, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7869-9793","authenticated-orcid":true,"given":"Vincent P","family":"Kunze","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}],"name":"Unit of Retinal Neurophysiology, National Eye Institute, National Institutes of Health","place":["Bethesda, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura K","family":"Patak","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}],"name":"Unit of Retinal Neurophysiology, National Eye Institute, National Institutes of Health","place":["Bethesda, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailey","family":"Kim","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}],"name":"Unit of Retinal Neurophysiology, National Eye Institute, National Institutes of Health","place":["Bethesda, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1065-8215","authenticated-orcid":true,"given":"Katie","family":"Kindt","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04mhx6838","id-type":"ROR","asserted-by":"publisher"}],"name":"Section on Sensory Cell Development and Function, National Institute on Deafness and Other Communication Disorders, National Institutes of Health","place":["Bethesda, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2897-649X","authenticated-orcid":true,"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}],"name":"Unit of Retinal Neurophysiology, National Eye Institute, National Institutes of Health","place":["Bethesda, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2023,2,6]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"4182","DOI":"10.1002\/cne.22447","article-title":"Ontogeny of cone photoreceptor mosaics in zebrafish","volume":"518","author":"Allison","year":"2010","journal-title":"The Journal of Comparative Neurology"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"2023","DOI":"10.1073\/pnas.0809439106","article-title":"Tbx2b is required for ultraviolet photoreceptor cell specification during zebrafish retinal development","volume":"106","author":"Alvarez-Delfin","year":"2009","journal-title":"PNAS"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"744","DOI":"10.1038\/nrg.2016.127","article-title":"The origin and evolution of cell types","volume":"17","author":"Arendt","year":"2016","journal-title":"Nature Reviews. 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