{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:38:12Z","timestamp":1785425892875,"version":"3.56.0"},"reference-count":60,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2023,5,10]],"date-time":"2023-05-10T00:00:00Z","timestamp":1683676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,5,10]],"date-time":"2023-05-10T00:00:00Z","timestamp":1683676800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,5,10]],"date-time":"2023-05-10T00:00:00Z","timestamp":1683676800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DE025806"],"award-info":[{"award-number":["DE025806"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,5,9]]},"abstract":"<jats:p>Natriuretic peptide signaling has been implicated in a broad range of physiological processes, regulating blood volume and pressure, ventricular hypertrophy, fat metabolism, and long bone growth. Here, we describe a completely novel role for natriuretic peptide signaling in the control of neural crest (NC) and cranial placode (CP) progenitors formation. Among the components of this signaling pathway, we show that natriuretic peptide receptor 3 (Npr3) plays a pivotal role by differentially regulating two developmental programs through its dual function as clearance and signaling receptor. Using a combination of MO-based knockdowns, pharmacological inhibitors and rescue assays we demonstrate that Npr3 cooperate with guanylate cyclase natriuretic peptide receptor 1 (Npr1) and natriuretic peptides (Nppa\/Nppc) to regulate NC and CP formation, pointing at a broad requirement of this signaling pathway in early embryogenesis. We propose that Npr3 acts as a clearance receptor to regulate local concentrations of natriuretic peptides for optimal cGMP production through Npr1 activation, and as a signaling receptor to control cAMP levels through inhibition of adenylyl cyclase. The intracellular modulation of these second messengers therefore participates in the segregation of NC and CP cell populations.<\/jats:p>","DOI":"10.7554\/elife.84036","type":"journal-article","created":{"date-parts":[[2023,5,10]],"date-time":"2023-05-10T06:47:09Z","timestamp":1683701229000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.84036","source":"Crossref","is-referenced-by-count":9,"title":["Npr3 regulates neural crest and cranial placode progenitors formation through its dual function as clearance and signaling receptor"],"prefix":"10.7554","volume":"12","author":[{"given":"Arun","family":"Devotta","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0190ak572","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Pathobiology, New York University, College of Dentistry","place":["New York, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo","family":"Juraver-Geslin","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0190ak572","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Pathobiology, New York University, College of Dentistry","place":["New York, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Casey","family":"Griffin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0190ak572","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Pathobiology, New York University, College of Dentistry","place":["New York, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3259-2103","authenticated-orcid":true,"given":"Jean-Pierre","family":"Saint-Jeannet","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0190ak572","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Pathobiology, New York University, College of Dentistry","place":["New York, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2023,5,10]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2011.10","article-title":"NPR-A regulates self-renewal and Pluripotency of embryonic stem cells","volume":"2","author":"Abdelalim","year":"2011","journal-title":"Cell Death & Disease"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"1264","DOI":"10.1089\/scd.2011.0239","article-title":"NPR-C protects embryonic stem cells from apoptosis by regulating P53 levels","volume":"21","author":"Abdelalim","year":"2012","journal-title":"Stem Cells and Development"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"19324","DOI":"10.1074\/jbc.271.32.19324","article-title":"Cytoplasmic domain of natriuretic peptide receptor-C inhibits Adenylyl cyclase. involvement of a pertussis toxin-sensitive G protein","volume":"271","author":"Anand-Srivastava","year":"1996","journal-title":"The Journal of Biological Chemistry"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/s0012-1606(03)00161-1","article-title":"Sox10 regulates the development of neural crest-derived Melanocytes in Xenopus","volume":"259","author":"Aoki","year":"2003","journal-title":"Developmental Biology"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1016\/j.ydbio.2013.12.011","article-title":"Identification of Pax3 and Zic1 targets in the developing neural crest","volume":"386","author":"Bae","year":"2014","journal-title":"Developmental Biology"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/B978-0-12-401730-6.00002-8","volume-title":"Neural Crest Cells: Evolution, Development and Disease","author":"Bae","year":"2014"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1242\/dev.100370","article-title":"Differential activation of natriuretic peptide receptors modulates cardiomyocyte proliferation during development","volume":"141","author":"Becker","year":"2014","journal-title":"Development"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"334","DOI":"10.1242\/dev.143354","article-title":"Osteocrin, a peptide secreted from the heart and other tissues, contributes to cranial Osteogenesis and Chondrogenesis in Zebrafish","volume":"144","author":"Chiba","year":"2017","journal-title":"Development"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"9863","DOI":"10.1074\/jbc.271.16.9863","article-title":"Molecular determinants of the clearance function of type C receptors of natriuretic peptides","volume":"271","author":"Cohen","year":"1996","journal-title":"Journal of Biological Chemistry"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"270","DOI":"10.1002\/ajmg.a.33702","article-title":"Cranial neural crest cells on the move: Their roles in Craniofacial development","volume":"155A","author":"Cordero","year":"2011","journal-title":"American Journal of Medical Genetics. 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