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Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    is thought to participate in taste sensation through an unknown mechanism. Here, we describe Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    ion binding and the response of taste receptor type 1 (T1r), a receptor family composing sweet\/umami receptors. The T1r2a\/T1r3 heterodimer from the medaka fish, currently the sole T1r amenable to structural analyses, exhibited a specific Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    binding in the vicinity of the amino-acid-binding site in the ligand-binding domain (LBD) of T1r3, which is likely conserved across species, including human T1r3. The Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    binding induced a conformational change in T1r2a\/T1r3LBD at sub- to low-mM concentrations, similar to canonical taste substances. Furthermore, oral Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    application to mice increased impulse frequencies of taste nerves connected to T1r-expressing taste cells and promoted their behavioral preferences attenuated by a T1r-specific blocker or T1r3 knock-out. These results suggest that the Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    evokes taste sensations by binding to T1r, thereby serving as another preferred salt taste pathway at a low concentration.\n                  <\/jats:p>","DOI":"10.7554\/elife.84291","type":"journal-article","created":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T07:15:59Z","timestamp":1675235759000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.84291","source":"Crossref","is-referenced-by-count":27,"title":["Chloride ions evoke taste sensations by binding to the extracellular ligand-binding domain of sweet\/umami taste receptors"],"prefix":"10.7554","volume":"12","author":[{"given":"Nanako","family":"Atsumi","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02pc6pc55","id-type":"ROR","asserted-by":"publisher"}],"name":"Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University","place":["Okayama, 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