{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T20:40:06Z","timestamp":1787863206952,"version":"build-2784847793"},"reference-count":39,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T00:00:00Z","timestamp":1681430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T00:00:00Z","timestamp":1681430400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T00:00:00Z","timestamp":1681430400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["GNT1152807"],"award-info":[{"award-number":["GNT1152807"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["GNT1196396"],"award-info":[{"award-number":["GNT1196396"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,4,13]]},"abstract":"<jats:sec id=\"abs1\">\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      Buruli ulcer (BU) is a neglected tropical disease caused by infection of subcutaneous tissue with\n                      <jats:italic>Mycobacterium ulcerans<\/jats:italic>\n                      . BU is commonly reported across rural regions of Central and West Africa but has been increasing dramatically in temperate southeast Australia around the major metropolitan city of Melbourne, with most disease transmission occurring in the summer months. Previous research has shown that Australian native possums are reservoirs of\n                      <jats:italic>M. ulcerans<\/jats:italic>\n                      and that they shed the bacteria in their fecal material (excreta). Field surveys show that locales where possums harbor\n                      <jats:italic>M. ulcerans<\/jats:italic>\n                      overlap with human cases of BU, raising the possibility of using possum excreta surveys to predict the risk of disease occurrence in humans.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"abs2\">\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      We thus established a highly structured 12 month possum excreta surveillance program across an area of 350 km\n                      <jats:sup>2<\/jats:sup>\n                      in the Mornington Peninsula area 70 km south of Melbourne, Australia. The primary objective of our study was to assess using statistical modeling if\n                      <jats:italic>M. ulcerans<\/jats:italic>\n                      surveillance of possum excreta provided useful information for predicting future human BU case locations.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"abs3\">\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Over two sampling campaigns in summer and winter, we collected 2,282 possum excreta specimens of which 11% were PCR positive for\n                      <jats:italic>M. ulcerans<\/jats:italic>\n                      -specific DNA. Using the spatial scanning statistical tool\n                      <jats:italic>SaTScan<\/jats:italic>\n                      , we observed non-random, co-correlated clustering of both\n                      <jats:italic>M. ulcerans<\/jats:italic>\n                      positive possum excreta and human BU cases. We next trained a statistical model with the Mornington Peninsula excreta survey data to predict the future likelihood of human BU cases occurring in the region. By observing where human BU cases subsequently occurred, we show that the excreta model performance was superior to a null model trained using the previous year\u2019s human BU case incidence data (AUC 0.66 vs 0.55). We then used data unseen by the excreta-informed model from a new survey of 661 possum excreta specimens in Geelong, a geographically separate BU endemic area to the southwest of Melbourne, to prospectively predict the location of human BU cases in that region. As for the Mornington Peninsula, the excreta-based BU prediction model outperformed the null model (AUC 0.75 vs 0.50) and pinpointed specific locations in Geelong where interventions could be deployed to interrupt disease spread.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"abs4\">\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      This study highlights the\n                      <jats:italic>One Health<\/jats:italic>\n                      nature of BU by confirming a quantitative relationship between possum excreta shedding of\n                      <jats:italic>M. ulcerans<\/jats:italic>\n                      and humans developing BU. The excreta survey-informed modeling we have described will be a powerful tool for the efficient targeting of public health responses to stop BU.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"abs5\">\n                    <jats:title>Funding:<\/jats:title>\n                    <jats:p>This research was supported by the National Health and Medical Research Council of Australia and the Victorian Government Department of Health (GNT1152807 and GNT1196396).<\/jats:p>\n                  <\/jats:sec>","DOI":"10.7554\/elife.84983","type":"journal-article","created":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T08:00:52Z","timestamp":1681459252000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.84983","source":"Crossref","is-referenced-by-count":26,"title":["Statistical modeling based on structured surveys of Australian native possum excreta harboring Mycobacterium ulcerans predicts Buruli ulcer occurrence in 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