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The large fusion GTPase Sey1\/atlastin is implicated in ER dynamics, ER-derived lipid droplet (LD) formation, and LCV maturation. Here, we employ cryo-electron tomography, confocal microscopy, proteomics, and isotopologue profiling to analyze LCV-LD interactions in the genetically tractable amoeba\n                    <jats:italic>Dictyostelium discoideum<\/jats:italic>\n                    . Dually fluorescence-labeled\n                    <jats:italic>D. discoideum<\/jats:italic>\n                    producing LCV and LD markers revealed that Sey1 as well as the\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    T4SS and the Ran GTPase activator LegG1 promote LCV-LD interactions. In vitro reconstitution using purified LCVs and LDs from parental or \u0394\n                    <jats:italic>sey1<\/jats:italic>\n                    mutant\n                    <jats:italic>D. discoideum<\/jats:italic>\n                    indicated that Sey1 and GTP promote this process. Sey1 and the\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    fatty acid transporter FadL were implicated in palmitate catabolism and palmitate-dependent intracellular growth. Taken together, our results reveal that Sey1 and LegG1 mediate LD- and FadL-dependent fatty acid metabolism of intracellular\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.7554\/elife.85142","type":"journal-article","created":{"date-parts":[[2023,5,9]],"date-time":"2023-05-09T09:36:27Z","timestamp":1683624987000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.85142","source":"Crossref","is-referenced-by-count":17,"title":["The large GTPase Sey1\/atlastin mediates lipid droplet- and FadL-dependent intracellular fatty acid metabolism of Legionella pneumophila"],"prefix":"10.7554","volume":"12","author":[{"given":"Dario","family":"H\u00fcsler","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02crff812","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Medical Microbiology, University of Z\u00fcrich","place":["Z\u00fcrich, Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5742-8362","authenticated-orcid":true,"given":"Pia","family":"Stauffer","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02crff812","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Medical Microbiology, University of Z\u00fcrich","place":["Z\u00fcrich, Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Keller","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02crff812","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Medical Microbiology, University of Z\u00fcrich","place":["Z\u00fcrich, Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desir\u00e9e","family":"B\u00f6ck","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05a28rw58","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Molecular Biology and Biophysics, ETH Z\u00fcrich","place":["Z\u00fcrich, Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Steiner","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02kkvpp62","id-type":"ROR","asserted-by":"publisher"}],"name":"Bavarian NMR Center - 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