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While some studies have examined adult physiology across elevations, relatively little attention has been given to the structure and function of eggshells despite their critical role in regulating gas exchange during the vulnerable embryonic stage. At high elevations, dry air is expected to increase water loss from the egg, and natural selection may favor lower gas conductance to reduce desiccation risk. Structural variation in eggshells, such as increased shell thickness or reduced pore size and density, could serve as a mechanism to regulate gas diffusion. To test for adaptive variation in eggshell traits along elevational gradients, we measured water vapor conductance and used scanning electron microscopy (SEM) to examine eggshell structure in 197 bird species from the Andes. We found that water vapor conductance declined at high elevations across avian communities. However, structural changes in eggshells varied among bird families and did not vary in a predictable way with elevation, suggesting no relationship or divergent adaptive responses to shared selective pressures, particularly in shell thickness, pore density, and pore size. We propose that examining functional and structural eggshell traits can offer insight into species\u2019 elevational limits and inform predictions about their responses to climate change.<\/jats:p>","DOI":"10.7554\/elife.85564","type":"journal-article","created":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T07:15:05Z","timestamp":1776150905000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.85564","source":"Crossref","is-referenced-by-count":0,"title":["Adaptive variation in avian eggshell gas conductance and structure across elevational gradients?"],"prefix":"10.7554","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1597-4038","authenticated-orcid":true,"given":"David","family":"Ocampo","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00hx57361","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Ecology and Evolutionary Biology, Princeton University","place":["Princeton, United 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