{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:54:32Z","timestamp":1785412472061,"version":"3.56.0"},"reference-count":55,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2023,7,20]],"date-time":"2023-07-20T00:00:00Z","timestamp":1689811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,7,20]],"date-time":"2023-07-20T00:00:00Z","timestamp":1689811200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,7,20]],"date-time":"2023-07-20T00:00:00Z","timestamp":1689811200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"crossref","award":["2020-67015-31017"],"award-info":[{"award-number":["2020-67015-31017"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>The degradation of sperm-borne mitochondria after fertilization is a conserved event. This process known as post-fertilization sperm mitophagy, ensures exclusively maternal inheritance of the mitochondria-harbored mitochondrial DNA genome. This mitochondrial degradation is in part carried out by the ubiquitin-proteasome system. In mammals, ubiquitin-binding pro-autophagic receptors such as SQSTM1 and GABARAP have also been shown to contribute to sperm mitophagy. These systems work in concert to ensure the timely degradation of the sperm-borne mitochondria after fertilization. We hypothesize that other receptors, cofactors, and substrates are involved in post-fertilization mitophagy. Mass spectrometry was used in conjunction with a porcine cell-free system to identify other autophagic cofactors involved in post-fertilization sperm mitophagy. This porcine cell-free system is able to recapitulate early fertilization proteomic interactions. Altogether, 185 proteins were identified as statistically different between control and cell-free-treated spermatozoa. Six of these proteins were further investigated, including MVP, PSMG2, PSMA3, FUNDC2, SAMM50, and BAG5. These proteins were phenotyped using porcine in vitro fertilization, cell imaging, proteomics, and the porcine cell-free system. The present data confirms the involvement of known mitophagy determinants in the regulation of mitochondrial inheritance and provides a master list of candidate mitophagy co-factors to validate in the future hypothesis-driven studies.<\/jats:p>","DOI":"10.7554\/elife.85596","type":"journal-article","created":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T05:59:10Z","timestamp":1679637550000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.85596","source":"Crossref","is-referenced-by-count":3,"title":["Identification of candidate mitochondrial inheritance determinants using the mammalian cell-free system"],"prefix":"10.7554","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5363-0407","authenticated-orcid":true,"given":"Dalen","family":"Zuidema","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02ymw8z06","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Animal Sciences, University of Missouri","place":["Columbia, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexis","family":"Jones","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02ymw8z06","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Animal Sciences, University of Missouri","place":["Columbia, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won-Hee","family":"Song","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02ymw8z06","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Animal Sciences, University of Missouri","place":["Columbia, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2964-5940","authenticated-orcid":true,"given":"Michal","family":"Zigo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02ymw8z06","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Animal Sciences, University of Missouri","place":["Columbia, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9231-2823","authenticated-orcid":true,"given":"Peter","family":"Sutovsky","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02ymw8z06","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Animal Sciences, University of Missouri","place":["Columbia, United States"]},{"id":[{"id":"https:\/\/ror.org\/02ymw8z06","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Obstetrics, Gynecology and Women\u2019s Health, University of Missouri","place":["Columbia, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2023,7,20]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202009092","article-title":"Samm50 acts with P62 in piecemeal Basal- and OXPHOS-induced Mitophagy of SAM and MICOS components","volume":"220","author":"Abudu","year":"2021","journal-title":"The Journal of Cell Biology"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"1144","DOI":"10.1126\/science.1211878","article-title":"Postfertilization Autophagy of sperm organelles prevents paternal mitochondrial DNA transmission","volume":"334","author":"Al Rawi","year":"2011","journal-title":"Science"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1038\/290457a0","article-title":"Sequence and organization of the human mitochondrial genome","volume":"290","author":"Anderson","year":"1981","journal-title":"Nature"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1002\/prca.200601013","article-title":"Identification of gene products present in Triton X-100 soluble and insoluble fractions of human Spermatozoa Lysates using LC-MS\/MS analysis","volume":"1","author":"Baker","year":"2007","journal-title":"Proteomics. Clinical Applications"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"907","DOI":"10.1038\/s41419-019-2132-x","article-title":"Bcl-2-associated athanogene 5 (BAG5) regulates Parkin-dependent mitophagy and cell death","volume":"10","author":"De Snoo","year":"2019","journal-title":"Cell Death & Disease"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1101\/gad.367606","article-title":"Mammalian Sperm translate nuclear-encoded proteins by mitochondrial-type Ribosomes","volume":"20","author":"Gur","year":"2006","journal-title":"Genes & Development"},{"key":"bib7","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2023.1116891","article-title":"Modulatory effect of MG-132 Proteasomal inhibition on boar Sperm motility during in vitro Capacitation","volume":"10","author":"Hackerova","year":"2023","journal-title":"Frontiers in Veterinary Science"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"553","DOI":"10.1016\/j.ceca.2006.08.016","article-title":"Mitochondrial calcium signalling and cell death: approaches for assessing the role of mitochondrial Ca2+ uptake in apoptosis","volume":"40","author":"Hajn\u00f3czky","year":"2006","journal-title":"Cell Calcium"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"452","DOI":"10.1016\/0012-1606(84)90210-0","article-title":"Maternal inheritance of the Mouse mitochondrial genome is not mediated by a loss or gross alteration of the paternal mitochondrial DNA or by methylation of the Oocyte mitochondrial DNA","volume":"102","author":"Hecht","year":"1984","journal-title":"Developmental Biology"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"853","DOI":"10.1095\/biolreprod.104.028134","article-title":"Regulation of Ubiquitin-Proteasome pathway on pig Oocyte Meiotic maturation and Fertilization1","volume":"71","author":"Huo","year":"2004","journal-title":"Biology of Reproduction"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"2989","DOI":"10.1016\/j.celrep.2018.05.015","article-title":"Sam50 regulates Pink1-Parkin-mediated Mitophagy by controlling Pink1 stability and mitochondrial morphology","volume":"23","author":"Jian","year":"2018","journal-title":"Cell Reports"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"931","DOI":"10.1016\/j.neuron.2004.11.026","article-title":"Bag5 inhibits Parkin and enhances dopaminergic neuron degeneration","volume":"44","author":"Kalia","year":"2004","journal-title":"Neuron"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"4542","DOI":"10.1073\/pnas.92.10.4542","article-title":"Elimination of paternal mitochondrial DNA in Intraspecific crosses during early Mouse Embryogenesis","volume":"92","author":"Kaneda","year":"1995","journal-title":"PNAS"},{"key":"bib14","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2156-8-8","article-title":"A persistent mitochondrial deletion reduces fitness and sperm performance in Heteroplasmic populations of C. elegans","volume":"8","author":"Liau","year":"2007","journal-title":"BMC Genetics"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"1313","DOI":"10.1002\/1873-3468.12170","article-title":"Samm50 affects mitochondrial morphology through the Association of Drp1 in mammalian cells","volume":"590","author":"Liu","year":"2016","journal-title":"FEBS Letters"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"13039","DOI":"10.1073\/pnas.1810946115","article-title":"Biparental inheritance of mitochondrial DNA in humans","volume":"115","author":"Luo","year":"2018","journal-title":"PNAS"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1038\/s41418-018-0121-8","article-title":"Mitochondrial Pip3-binding protein Fundc2 supports platelet survival via AKT signaling pathway","volume":"26","author":"Ma","year":"2019","journal-title":"Cell Death and Differentiation"},{"key":"bib18","doi-asserted-by":"publisher","first-page":"4356","DOI":"10.1002\/pmic.200600094","article-title":"Proteomic identification of human Sperm proteins","volume":"6","author":"Mart\u00ednez-Heredia","year":"2006","journal-title":"PROTEOMICS"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"R551","DOI":"10.1016\/j.cub.2006.06.054","article-title":"Mitochondria: more than just a powerhouse","volume":"16","author":"McBride","year":"2006","journal-title":"Current Biology"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/S0070-2153(06)77005-3","article-title":"Mitochondrial disease--its impact, etiology, and pathology","volume":"77","author":"McFarland","year":"2007","journal-title":"Current Topics in Developmental Biology"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"1268","DOI":"10.1002\/mrd.20691","article-title":"Reprogramming events of mammalian somatic cells induced by Xenopus laevis egg extracts","volume":"74","author":"Miyamoto","year":"2007","journal-title":"Molecular Reproduction and Development"},{"key":"bib22","doi-asserted-by":"publisher","first-page":"935","DOI":"10.1095\/biolreprod.108.073676","article-title":"Cell-free extracts from mammalian Oocytes partially induce nuclear Reprogramming in somatic cells","volume":"80","author":"Miyamoto","year":"2009","journal-title":"Biology of Reproduction"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"7458","DOI":"10.1038\/sj.onc.1206947","article-title":"Vaults: a Ribonucleoprotein particle involved in drug resistance","volume":"22","author":"Mossink","year":"2003","journal-title":"Oncogene"},{"key":"bib24","doi-asserted-by":"publisher","first-page":"1173","DOI":"10.1128\/MCB.06388-11","article-title":"Sam50 functions in mitochondrial Intermembrane space bridging and Biogenesis of respiratory complexes","volume":"32","author":"Ott","year":"2012","journal-title":"Molecular and Cellular Biology"},{"key":"bib25","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4396","article-title":"Quantitative live-cell imaging reveals Spatio-temporal Dynamics and cytoplasmic assembly of the 26S Proteasome","volume":"5","author":"Pack","year":"2014","journal-title":"Nature Communications"},{"key":"bib26","doi-asserted-by":"publisher","first-page":"1013","DOI":"10.1038\/s41556-018-0176-2","article-title":"Mechanisms of Mitophagy in cellular homeostasis, physiology and pathology","volume":"20","author":"Palikaras","year":"2018","journal-title":"Nature Cell Biology"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"1609","DOI":"10.1016\/j.fertnstert.2014.03.011","article-title":"Acute Epididymitis induces alterations in Sperm protein composition","volume":"101","author":"Pilatz","year":"2014","journal-title":"Fertility and Sterility"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1016\/j.devcel.2014.04.005","article-title":"Paternal mitochondrial destruction after fertilization is mediated by a common Endocytic and Autophagic pathway in Drosophila","volume":"29","author":"Politi","year":"2014","journal-title":"Developmental Cell"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"1141","DOI":"10.1126\/science.1210333","article-title":"Degradation of paternal mitochondria by fertilization-triggered Autophagy in C. elegans embryos","volume":"334","author":"Sato","year":"2011","journal-title":"Science"},{"key":"bib30","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.116.142190","article-title":"Spermbase: A database for sperm-borne RNA contents","volume":"95","author":"Schuster","year":"2016","journal-title":"Biology of Reproduction"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"576","DOI":"10.1056\/NEJMoa020350","article-title":"Paternal inheritance of mitochondrial DNA","volume":"347","author":"Schwartz","year":"2002","journal-title":"The New England Journal of Medicine"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1016\/j.cell.2012.09.004","article-title":"Heteroplasmy of Mouse mtDNA is genetically unstable and results in altered behavior and cognition","volume":"151","author":"Sharpley","year":"2012","journal-title":"Cell"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"851","DOI":"10.1093\/genetics\/148.2.851","article-title":"Maternal inheritance of Mouse mtDNA in Interspecific hybrids: segregation of the leaked paternal mtDNA followed by the prevention of subsequent paternal leakage","volume":"148","author":"Shitara","year":"1998","journal-title":"Genetics"},{"key":"bib34","doi-asserted-by":"publisher","DOI":"10.1101\/2020.02.26.939405","article-title":"Heteroplasmy variability in individuals with biparentally inherited mitochondrial DNA","volume-title":"bioRxiv","author":"Slone","year":"2020"},{"key":"bib35","doi-asserted-by":"publisher","first-page":"E5261","DOI":"10.1073\/pnas.1605844113","article-title":"Autophagy and ubiquitin\u2013proteasome system contribute to sperm mitophagy after mammalian fertilization","volume":"113","author":"Song","year":"2016","journal-title":"PNAS"},{"key":"bib36","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1007\/978-1-4939-8748-1","article-title":"Porcine cell-free system to study mammalian Sperm Mitophagy","volume":"1854","author":"Song","year":"2018","journal-title":"Methods in Molecular Biology"},{"key":"bib37","doi-asserted-by":"publisher","DOI":"10.3390\/cells10092450","article-title":"Mammalian cell-free system Recapitulates the early events of post-fertilization Sperm Mitophagy","volume":"10","author":"Song","year":"2021","journal-title":"Cells"},{"key":"bib38","doi-asserted-by":"publisher","first-page":"14447","DOI":"10.1021\/bi026747e","article-title":"Vault Ribonucleoprotein particles: Sarcophagi, Gondolas, or safety deposit boxes","volume":"41","author":"Suprenant","year":"2002","journal-title":"Biochemistry"},{"key":"bib39","doi-asserted-by":"publisher","first-page":"2841","DOI":"10.1242\/jcs.111.19.2841","article-title":"Assembly of nuclear pore complexes and Annulate Lamellae promotes normal Pronuclear development in fertilized mammalian Oocytes","volume":"111 (Pt 19)","author":"Sutovsky","year":"1998","journal-title":"Journal of Cell Science"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1038\/46466","article-title":"Ubiquitin tag for Sperm mitochondria","volume":"402","author":"Sutovsky","year":"1999","journal-title":"Nature"},{"key":"bib41","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/s0074-7696(08)62703-5","article-title":"Paternal contributions to the mammalian Zygote: fertilization after Sperm-egg fusion","volume":"195","author":"Sutovsky","year":"2000","journal-title":"International Review of Cytology"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"1793","DOI":"10.1095\/biolreprod.102.012799","article-title":"Early degradation of paternal mitochondria in domestic pig (Sus Scrofa) is prevented by selective Proteasomal inhibitors Lactacystin and Mg132","volume":"68","author":"Sutovsky","year":"2003","journal-title":"Biology of Reproduction"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1385\/1-59259-744-0:059","article-title":"Visualization of sperm accessory structures in the mammalian Spermatids, Spermatozoa, and Zygotes by Immunofluorescence, confocal, and Immunoelectron microscopy","volume":"253","author":"Sutovsky","year":"2004","journal-title":"Methods in Molecular Biology"},{"key":"bib44","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1016\/s1472-6483(10)60495-6","article-title":"Degradation of paternal mitochondria after fertilization: implications for Heteroplasmy, assisted reproductive technologies and mtDNA inheritance","volume":"8","author":"Sutovsky","year":"2004","journal-title":"Reproductive Biomedicine Online"},{"key":"bib45","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1530\/rep.1.00291","article-title":"Expression and Proteasomal degradation of the major vault protein (MVP) in mammalian Oocytes and Zygotes","volume":"129","author":"Sutovsky","year":"2005","journal-title":"Reproduction"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1530\/REP-11-0041","article-title":"Sperm Proteasome and fertilization","volume":"142","author":"Sutovsky","year":"2011","journal-title":"Reproduction"},{"key":"bib47","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0086276","article-title":"Bag5 protects against mitochondrial oxidative damage through regulating Pink1 degradation","volume":"9","author":"Wang","year":"2014","journal-title":"PLOS ONE"},{"key":"bib48","doi-asserted-by":"publisher","first-page":"839","DOI":"10.1111\/gtc.12631","article-title":"Pac1-Pac2 Proteasome assembly chaperone retains the core \u03914-\u03917 assembly Intermediates in the cytoplasm","volume":"23","author":"Wu","year":"2018","journal-title":"Genes to Cells"},{"key":"bib49","doi-asserted-by":"publisher","first-page":"3545","DOI":"10.1016\/j.febslet.2007.06.052","article-title":"The mitochondrial inner membrane protein Mitofilin exists as a complex with Sam50, Metaxins 1 and 2, coiled-coil-Helix coiled-coil-Helix domain-containing protein 3 and 6 And Dnajc11","volume":"581","author":"Xie","year":"2007","journal-title":"FEBS Letters"},{"key":"bib50","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1007\/s00441-010-0988-2","article-title":"Interference with the 19S Proteasomal regulatory complex subunit Psmd4 on the Sperm surface inhibits Sperm-Zona Pellucida penetration during porcine fertilization","volume":"341","author":"Yi","year":"2010","journal-title":"Cell and Tissue Research"},{"key":"bib51","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.836208","article-title":"High resolution Proteomic analysis of subcellular fractionated boar Spermatozoa provides comprehensive insights into Perinuclear Theca-residing proteins","volume":"10","author":"Zhang","year":"2022","journal-title":"Frontiers in Cell and Developmental Biology"},{"key":"bib52","doi-asserted-by":"publisher","first-page":"1662","DOI":"10.1038\/cr.2011.182","article-title":"Elimination of paternal mitochondria through the lysosomal degradation pathway in C. elegans","volume":"21","author":"Zhou","year":"2011","journal-title":"Cell Research"},{"key":"bib53","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-49024-0","article-title":"Compartmentalization of the Proteasome-interacting proteins during Sperm Capacitation","volume":"9","author":"Zigo","year":"2019","journal-title":"Scientific Reports"},{"key":"bib54","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017256","article-title":"Sperm Proteasomes degrade Sperm receptor on the egg Zona Pellucida during mammalian fertilization","volume":"6","author":"Zimmerman","year":"2011","journal-title":"PLOS ONE"},{"key":"bib55","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1007\/s00441-019-03100-z","article-title":"The domestic pig as a model for the study of mitochondrial inheritance","volume":"380","author":"Zuidema","year":"2019","journal-title":"Cell and Tissue Research"}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/85596\/elife-85596-v1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/85596\/elife-85596-v1.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/85596","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T19:06:30Z","timestamp":1697223990000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/85596"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,20]]},"references-count":55,"alternative-id":["10.7554\/eLife.85596"],"URL":"https:\/\/doi.org\/10.7554\/elife.85596","archive":["CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.7554\/eLife.85596.2","asserted-by":"subject"},{"id-type":"doi","id":"10.1101\/2023.01.23.525177","asserted-by":"subject"},{"id-type":"doi","id":"10.1101\/2023.01.23.525177","asserted-by":"object"}],"is-same-as":[{"id-type":"doi","id":"10.7554\/eLife.85596.3","asserted-by":"subject"}],"is-version-of":[{"id-type":"doi","id":"10.7554\/eLife.85596.1","asserted-by":"subject"},{"id-type":"doi","id":"10.7554\/eLife.85596.2","asserted-by":"subject"}],"is-supplemented-by":[{"id-type":"doi","id":"10.5061\/dryad.t4b8gtj5v","asserted-by":"subject"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,20]]},"assertion":[{"value":"single anonymised","name":"peer_review_transparency","label":"Peer review transparency","group":{"name":"peer_review_taxonomy"}},{"value":"other reviewer(s), editor","name":"peer_review_interaction","label":"Peer review interaction","group":{"name":"peer_review_taxonomy"}},{"value":"review summaries, review reports, author\/editor communication, reviewer identities reviewer opt in, editor identities","name":"peer_review_published","label":"Peer review published","group":{"name":"peer_review_taxonomy"}},{"value":"open (sign in with ORCID iD required)","name":"post_publication_commenting","label":"Post publication commenting","group":{"name":"post_publication_commenting"}}],"article-number":"RP85596"}}