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The regulation of these traits often requires precise control of hormone levels across diverse timescales. However, fine tuning circulating hormones in-vivo has traditionally been experimentally challenging. Here, using the naturally short-lived turquoise killifish (\n                    <jats:italic>N. furzeri<\/jats:italic>\n                    ), we describe a high-throughput platform that combines loss- and gain-of-function of peptide hormones. Mutation of three primary pituitary hormones, growth hormone (\n                    <jats:italic>gh1<\/jats:italic>\n                    ), follicle stimulating hormone (\n                    <jats:italic>fshb<\/jats:italic>\n                    ), and thyroid stimulating hormone (\n                    <jats:italic>tshb<\/jats:italic>\n                    ), alters somatic growth and reproduction. Thus, suggesting that while the killifish undergoes extremely rapid growth and maturity, it still relies on vertebrate-conserved genetic networks. As the next stage, we developed a gain-of-function vector system in which a hormone is tagged using a self-cleavable fluorescent reporter, and ectopically expressed in-vivo through intramuscular electroporation. Following a single electroporation, phenotypes, such as reproduction, are stably rescued for several months. Notably, we demonstrate the versatility of this approach by using multiplexing, dose-dependent, and doxycycline-inducible systems to achieve tunable and reversible expression. In summary, this method is relatively high-throughput, and facilitates large-scale interrogation of life-history strategies in fish. Ultimately, this approach could be adapted for modifying aquaculture species and exploring pro-longevity interventions.\n                  <\/jats:p>","DOI":"10.7554\/elife.85960","type":"journal-article","created":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T06:15:17Z","timestamp":1695017717000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.85960","source":"Crossref","is-referenced-by-count":15,"title":["A scalable and tunable platform for functional interrogation of peptide hormones in fish"],"prefix":"10.7554","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0090-335X","authenticated-orcid":true,"given":"Eitan","family":"Moses","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03qxff017","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Genetics, the Silberman Institute, The Hebrew University of Jerusalem","place":["Jerusalem, 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