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The glideosome-associated connector (GAC) is a conserved protein essential to this process. GAC facilitates the association of actin filaments with surface transmembrane adhesins and the efficient transmission of the force generated by myosin translocation of actin to the cell surface substrate. Here, we present the crystal structure of\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    GAC and reveal a unique, supercoiled armadillo repeat region that adopts a closed ring conformation. Characterisation of the solution properties together with membrane and F-actin binding interfaces suggests that GAC adopts several conformations from closed to open and extended. A multi-conformational model for assembly and regulation of GAC within the glideosome is proposed.\n                  <\/jats:p>","DOI":"10.7554\/elife.86049","type":"journal-article","created":{"date-parts":[[2023,4,4]],"date-time":"2023-04-04T06:15:33Z","timestamp":1680588933000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.86049","source":"Crossref","is-referenced-by-count":16,"title":["Structural and regulatory insights into the glideosome-associated connector from Toxoplasma gondii"],"prefix":"10.7554","volume":"12","author":[{"given":"Amit","family":"Kumar","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/041kmwe10","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Life Sciences, Imperial College London","place":["London, United 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