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How these capillary types emerge in a brain region-specific manner and subsequently establish intra-brain vascular heterogeneity remains unclear. Here, we performed a comparative analysis of vascularization across the zebrafish choroid plexuses (CPs), circumventricular organs (CVOs), and retinal choroid, and show common angiogenic mechanisms critical for fenestrated brain capillary formation. We found that zebrafish deficient for Gpr124, Reck, or Wnt7aa exhibit severely impaired BBB angiogenesis without any apparent defect in fenestrated capillary formation in the CPs, CVOs, and retinal choroid. Conversely, genetic loss of various Vegf combinations caused significant disruptions in Wnt7\/Gpr124\/Reck signaling-independent vascularization of these organs. The phenotypic variation and specificity revealed heterogeneous endothelial requirements for Vegfs-dependent angiogenesis during CP and CVO vascularization, identifying unexpected interplay of Vegfc\/d and Vegfa in this process. Mechanistically, expression analysis and paracrine activity-deficient\n                    <jats:italic>vegfc<\/jats:italic>\n                    mutant characterization suggest that endothelial cells and non-neuronal specialized cell types present in the CPs and CVOs are major sources of Vegfs responsible for regionally restricted angiogenic interplay. Thus, brain region-specific presentations and interplay of Vegfc\/d and Vegfa control emergence of fenestrated capillaries, providing insight into the mechanisms driving intra-brain vascular heterogeneity and fenestrated vessel formation in other organs.\n                  <\/jats:p>","DOI":"10.7554\/elife.86066","type":"journal-article","created":{"date-parts":[[2023,5,16]],"date-time":"2023-05-16T06:15:20Z","timestamp":1684217720000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.86066","source":"Crossref","is-referenced-by-count":31,"title":["Local angiogenic interplay of Vegfc\/d and Vegfa controls brain region-specific emergence of fenestrated capillaries"],"prefix":"10.7554","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9932-5117","authenticated-orcid":true,"given":"Sweta","family":"Parab","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03xjacd83","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Neurosciences, Lerner 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