{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T19:23:32Z","timestamp":1791919412288,"version":"4.3.4"},"reference-count":78,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T00:00:00Z","timestamp":1690243200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T00:00:00Z","timestamp":1690243200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T00:00:00Z","timestamp":1690243200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["102645\/Z\/13\/Z"],"award-info":[{"award-number":["102645\/Z\/13\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["219433\/Z\/19\/Z"],"award-info":[{"award-number":["219433\/Z\/19\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"crossref","award":["BB\/N006119\/1"],"award-info":[{"award-number":["BB\/N006119\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"crossref","award":["BB\/P007996\/1"],"award-info":[{"award-number":["BB\/P007996\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Medical Research Council","award":["MR\/S002987\/1"],"award-info":[{"award-number":["MR\/S002987\/1"]}]},{"name":"Medical Research Council","award":["MR\/T01072X\/1"],"award-info":[{"award-number":["MR\/T01072X\/1"]}]},{"name":"Medical Research Council","award":["MR\/V033638\/1"],"award-info":[{"award-number":["MR\/V033638\/1"]}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Somatosensory information is processed by a complex network of interneurons in the spinal dorsal horn. It has been reported that inhibitory interneurons that express neuropeptide Y (NPY), either permanently or during development, suppress mechanical itch, with no effect on pain. Here, we investigate the role of interneurons that continue to express NPY (NPY-INs) in the adult mouse spinal cord. We find that chemogenetic activation of NPY-INs reduces behaviours associated with acute pain and pruritogen-evoked itch, whereas silencing them causes exaggerated itch responses that depend on cells expressing the gastrin-releasing peptide receptor. As predicted by our previous studies, silencing of another population of inhibitory interneurons (those expressing dynorphin) also increases itch, but to a lesser extent. Importantly, NPY-IN activation also reduces behavioural signs of inflammatory and neuropathic pain. These results demonstrate that NPY-INs gate pain and itch transmission at the spinal level, and therefore represent a potential treatment target for pathological pain and itch.<\/jats:p>","DOI":"10.7554\/elife.86633","type":"journal-article","created":{"date-parts":[[2023,3,30]],"date-time":"2023-03-30T03:48:23Z","timestamp":1680148103000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.86633","source":"Crossref","is-referenced-by-count":38,"title":["Neuropeptide Y-expressing dorsal horn inhibitory interneurons gate spinal pain and itch signalling"],"prefix":"10.7554","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3201-6149","authenticated-orcid":true,"given":"Kieran A","family":"Boyle","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00vtgdb53","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Psychology and Neuroscience, College of Medical, Veterinary and Life Sciences, University of Glasgow","place":["Glasgow, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika","family":"Polgar","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00vtgdb53","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Psychology and Neuroscience, College of Medical, Veterinary and Life Sciences, University of Glasgow","place":["Glasgow, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Gutierrez-Mecinas","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00vtgdb53","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Psychology and Neuroscience, College of Medical, Veterinary and Life Sciences, University of Glasgow","place":["Glasgow, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6339-2801","authenticated-orcid":true,"given":"Allen C","family":"Dickie","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00vtgdb53","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Psychology and Neuroscience, College of Medical, Veterinary and Life Sciences, University of Glasgow","place":["Glasgow, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4737-9364","authenticated-orcid":true,"given":"Andrew H","family":"Cooper","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00vtgdb53","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Psychology and Neuroscience, College of Medical, Veterinary and Life Sciences, University of Glasgow","place":["Glasgow, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6510-4423","authenticated-orcid":true,"given":"Andrew M","family":"Bell","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00vtgdb53","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Psychology and Neuroscience, College of Medical, Veterinary and Life Sciences, University of Glasgow","place":["Glasgow, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelline","family":"Jumolea","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00vtgdb53","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Psychology and Neuroscience, College of Medical, Veterinary and Life Sciences, University of Glasgow","place":["Glasgow, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4421-5551","authenticated-orcid":true,"given":"Adrian","family":"Casas-Benito","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00vtgdb53","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Psychology and Neuroscience, College of Medical, Veterinary and Life Sciences, University of Glasgow","place":["Glasgow, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5037-7138","authenticated-orcid":true,"given":"Masahiko","family":"Watanabe","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02e16g702","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Anatomy, Hokkaido University School of Medicine","place":["Sapporo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1260-3362","authenticated-orcid":true,"given":"David I","family":"Hughes","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00vtgdb53","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Psychology and Neuroscience, College of Medical, Veterinary and Life Sciences, University of Glasgow","place":["Glasgow, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory A","family":"Weir","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00vtgdb53","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Psychology and Neuroscience, College of Medical, Veterinary and Life Sciences, University of Glasgow","place":["Glasgow, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John S","family":"Riddell","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00vtgdb53","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Psychology and Neuroscience, College of Medical, Veterinary and Life Sciences, University of Glasgow","place":["Glasgow, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3007-6749","authenticated-orcid":true,"given":"Andrew J","family":"Todd","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00vtgdb53","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Psychology and Neuroscience, College of Medical, Veterinary and Life Sciences, University of Glasgow","place":["Glasgow, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2023,7,25]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"618","DOI":"10.1016\/j.neuron.2013.07.051","article-title":"The sensory neurons of touch","volume":"79","author":"Abraira","year":"2013","journal-title":"Neuron"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1016\/j.celrep.2019.06.033","article-title":"Spinal neuropeptide Y1 receptor-expressing neurons form an essential excitatory pathway for mechanical itch","volume":"28","author":"Acton","year":"2019","journal-title":"Cell Reports"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1016\/j.neuron.2022.10.008","article-title":"Inhibitory Kcnip2 neurons of the spinal dorsal horn control behavioral sensitivity to environmental cold","volume":"111","author":"Albisetti","year":"2023","journal-title":"Neuron"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1097\/j.pain.0000000000000753","article-title":"Peripheral neuropathic pain: a mechanism-related organizing principle based on sensory profiles","volume":"158","author":"Baron","year":"2017","journal-title":"Pain"},{"key":"bib5","doi-asserted-by":"publisher","DOI":"10.1177\/1744806916649602","article-title":"Spinal neurons that contain gastrin-releasing peptide seldom express Fos or phosphorylate extracellular signal-regulated kinases in response to intradermal chloroquine","volume":"12","author":"Bell","year":"2016","journal-title":"Molecular Pain"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"875","DOI":"10.1016\/0024-3205(85)90523-5","article-title":"Hyperalgesia induced by altered glycinergic activity at the spinal cord","volume":"37","author":"Beyer","year":"1985","journal-title":"Life Sciences"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1126\/science.aac8653","article-title":"Gate control of mechanical itch by a subpopulation of spinal cord interneurons","volume":"350","author":"Bourane","year":"2015","journal-title":"Science"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1016\/j.neuroscience.2017.08.044","article-title":"A quantitative study of neurochemically defined populations of inhibitory interneurons in the superficial dorsal horn of the mouse spinal cord","volume":"363","author":"Boyle","year":"2017","journal-title":"Neuroscience"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"526","DOI":"10.1016\/j.celrep.2019.06.040","article-title":"Defining a spinal microcircuit that gates myelinated afferent input: implications for tactile allodynia","volume":"28","author":"Boyle","year":"2019","journal-title":"Cell Reports"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"522","DOI":"10.1016\/j.neuron.2014.01.018","article-title":"Transmitting pain and itch messages: a contemporary view of the spinal cord circuits that generate gate control","volume":"82","author":"Braz","year":"2014","journal-title":"Neuron"},{"key":"bib11","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0039765","article-title":"A novel behavioral assay for measuring cold sensation in mice","volume":"7","author":"Brenner","year":"2012","journal-title":"PLOS ONE"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"1906","DOI":"10.1097\/j.pain.0000000000001884","article-title":"Postnatal maturation of spinal dynorphin circuits and their role in somatosensation","volume":"161","author":"Brewer","year":"2020","journal-title":"Pain"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1038\/nmeth.2450","article-title":"Improved tools for the Brainbow toolbox","volume":"10","author":"Cai","year":"2013","journal-title":"Nature Methods"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"2061","DOI":"10.1097\/j.pain.0000000000000270","article-title":"The organisation of spinoparabrachial neurons in the mouse","volume":"156","author":"Cameron","year":"2015","journal-title":"Pain"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"945","DOI":"10.1152\/physrev.00017.2019","article-title":"Physiology and pathophysiology of itch","volume":"100","author":"Cevikbas","year":"2020","journal-title":"Physiological Reviews"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/0165-0270(94)90144-9","article-title":"Quantitative assessment of tactile allodynia in the rat paw","volume":"53","author":"Chaplan","year":"1994","journal-title":"Journal of Neuroscience Methods"},{"key":"bib17","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18895-7","article-title":"A spinal neural circuitry for converting touch to itch sensation","volume":"11","author":"Chen","year":"2020","journal-title":"Nature Communications"},{"key":"bib18","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16859-5","article-title":"Central circuit mechanisms of itch","volume":"11","author":"Chen","year":"2020","journal-title":"Nature Communications"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1002\/jnr.24846","article-title":"Endogenous \u00b5-opioid receptor activity in the lateral and capsular subdivisions of the right central nucleus of the amygdala prevents chronic postoperative pain","volume":"100","author":"Cooper","year":"2022","journal-title":"Journal of Neuroscience Research"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1097\/j.pain.0000000000001406","article-title":"Morphological and functional properties distinguish the substance P and gastrin-releasing peptide subsets of excitatory interneuron in the spinal cord dorsal horn","volume":"160","author":"Dickie","year":"2019","journal-title":"Pain"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1146\/annurev.pa.20.040180.002301","article-title":"Efficient analysis of experimental observations","volume":"20","author":"Dixon","year":"1980","journal-title":"Annual Review of Pharmacology and Toxicology"},{"key":"bib22","doi-asserted-by":"publisher","first-page":"1417","DOI":"10.1016\/j.cell.2014.11.003","article-title":"Identification of spinal circuits transmitting and gating mechanical pain","volume":"159","author":"Duan","year":"2014","journal-title":"Cell"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1007\/BF01178229","article-title":"Quantitative and electron microscopic studies of sensory ganglion cells of the sprawling mouse","volume":"6","author":"Duchen","year":"1977","journal-title":"Journal of Neurocytology"},{"key":"bib24","doi-asserted-by":"publisher","first-page":"1289","DOI":"10.1016\/j.neuron.2015.02.028","article-title":"Targeted ablation, silencing, and activation establish glycinergic dorsal horn neurons as key components of a spinal gate for pain and itch","volume":"85","author":"Foster","year":"2015","journal-title":"Neuron"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"1325","DOI":"10.1111\/2041-210X.12584","article-title":"BORIS: a free, versatile open\u2010source event\u2010logging software for video\/audio coding and live observations","volume":"7","author":"Friard","year":"2016","journal-title":"Methods in Ecology and Evolution"},{"key":"bib26","doi-asserted-by":"publisher","first-page":"1368","DOI":"10.1016\/j.neuron.2013.10.016","article-title":"GENSAT BAC CRE-Recombinase driver lines to study the functional organization of cerebral cortical and basal ganglia circuits","volume":"80","author":"Gerfen","year":"2013","journal-title":"Neuron"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1126\/science.aan2475","article-title":"Chemogenetics revealed: DREADD occupancy and activation via converted clozapine","volume":"357","author":"Gomez","year":"2017","journal-title":"Science"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"440","DOI":"10.1097\/j.pain.0000000000000778","article-title":"Preprotachykinin A is expressed by A distinct population of excitatory neurons in the mouse superficial spinal dorsal horn including cells that respond to noxious and pruritic stimuli","volume":"158","author":"Gutierrez-Mecinas","year":"2017","journal-title":"Pain"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"869","DOI":"10.1038\/s41593-018-0141-1","article-title":"Neuronal atlas of the dorsal horn defines its architecture and links sensory input to transcriptional cell types","volume":"21","author":"H\u00e4ring","year":"2018","journal-title":"Nature Neuroscience"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1016\/s0306-4522(00)00110-x","article-title":"Murine models of inflammatory, neuropathic and cancer pain each generates a unique set of neurochemical changes in the spinal cord and sensory neurons","volume":"98","author":"Honore","year":"2000","journal-title":"Neuroscience"},{"key":"bib31","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-018-0149-6","article-title":"Circuit dissection of the role of somatostatin in itch and pain","volume":"21","author":"Huang","year":"2018","journal-title":"Nature Neuroscience"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"352","DOI":"10.1016\/j.pain.2007.09.016","article-title":"Intrathecal neuropeptide Y reduces behavioral and molecular markers of inflammatory or neuropathic pain","volume":"137","author":"Intondi","year":"2008","journal-title":"Pain"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"914","DOI":"10.1016\/j.neuroscience.2009.10.052","article-title":"Topography and time course of changes in spinal neuropeptide Y immunoreactivity after spared nerve injury","volume":"165","author":"Intondi","year":"2010","journal-title":"Neuroscience"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"598","DOI":"10.1097\/j.pain.0000000000000407","article-title":"A combined electrophysiological and morphological study of neuropeptide Y-expressing inhibitory interneurons in the spinal dorsal horn of the mouse","volume":"157","author":"Iwagaki","year":"2016","journal-title":"Pain"},{"key":"bib35","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1016\/j.neuron.2014.02.046","article-title":"Dynorphin acts as a neuromodulator to inhibit itch in the dorsal horn of the spinal cord","volume":"82","author":"Kardon","year":"2014","journal-title":"Neuron"},{"key":"bib36","doi-asserted-by":"publisher","first-page":"1364","DOI":"10.1038\/nn.2407","article-title":"Unmasking the tonic-aversive state in neuropathic pain","volume":"12","author":"King","year":"2009","journal-title":"Nature Neuroscience"},{"key":"bib37","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1146\/annurev-physiol-022516-034303","article-title":"Spinal circuits for touch, pain, and itch","volume":"80","author":"Koch","year":"2018","journal-title":"Annual Review of Physiology"},{"key":"bib38","doi-asserted-by":"publisher","DOI":"10.1177\/17448069221119614","article-title":"Characterisation of deep dorsal horn projection neurons in the spinal cord of the Phox2a::Cre mouse line","volume":"18","author":"K\u00f3kai","year":"2022","journal-title":"Molecular Pain"},{"key":"bib39","doi-asserted-by":"publisher","first-page":"1424","DOI":"10.1172\/JCI46229","article-title":"Rapid, reversible activation of AgRP neurons drives feeding behavior in mice","volume":"121","author":"Krashes","year":"2011","journal-title":"The Journal of Clinical Investigation"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1038\/nature12956","article-title":"An excitatory paraventricular nucleus to AgRP neuron circuit that drives hunger","volume":"507","author":"Krashes","year":"2014","journal-title":"Nature"},{"key":"bib41","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1016\/j.neulet.2016.12.015","article-title":"Pax2 is persistently expressed by GABAergic neurons throughout the adult rat dorsal horn","volume":"638","author":"Larsson","year":"2017","journal-title":"Neuroscience Letters"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1007\/BF01236123","article-title":"The postnatal development of large light and small dark neurons in mouse dorsal root ganglia: a statistical analysis of cell numbers and size","volume":"8","author":"Lawson","year":"1979","journal-title":"Journal of Neurocytology"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"27011","DOI":"10.1073\/pnas.1905658116","article-title":"Synaptic control of spinal GRPR+ neurons by local and long-range inhibitory inputs","volume":"116","author":"Liu","year":"2019","journal-title":"PNAS"},{"key":"bib44","doi-asserted-by":"publisher","first-page":"3900","DOI":"10.1523\/JNEUROSCI.0102-05.2005","article-title":"Modular organization of excitatory circuits between neurons of the spinal superficial dorsal horn (laminae I and II)","volume":"25","author":"Lu","year":"2005","journal-title":"The Journal of Neuroscience"},{"key":"bib45","doi-asserted-by":"publisher","first-page":"4050","DOI":"10.1172\/JCI70026","article-title":"A feed-forward spinal cord glycinergic neural circuit gates mechanical allodynia","volume":"123","author":"Lu","year":"2013","journal-title":"The Journal of Clinical Investigation"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1002\/cne.25232","article-title":"Morphological and neurochemical characterization of glycinergic neurons in laminae I-IV of the mouse spinal dorsal horn","volume":"530","author":"Miranda","year":"2022","journal-title":"The Journal of Comparative Neurology"},{"key":"bib47","doi-asserted-by":"publisher","first-page":"968","DOI":"10.1126\/science.1233765","article-title":"The cells and circuitry for itch responses in mice","volume":"340","author":"Mishra","year":"2013","journal-title":"Science"},{"key":"bib48","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1007\/978-3-662-44605-8_8","volume-title":"Handbook of Experimental Pharmacology","author":"Mishra","year":"2015"},{"key":"bib49","doi-asserted-by":"publisher","first-page":"695","DOI":"10.1126\/science.aaf4918","article-title":"A central neural circuit for itch sensation","volume":"357","author":"Mu","year":"2017","journal-title":"Science"},{"key":"bib50","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-43493-z","article-title":"Facilitation of neuropathic pain by the NPY Y1 receptor-expressing subpopulation of excitatory interneurons in the dorsal horn","volume":"9","author":"Nelson","year":"2019","journal-title":"Scientific Reports"},{"key":"bib51","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2020.101894","article-title":"Targeting spinal neuropeptide Y1 receptor-expressing interneurons to alleviate chronic pain and itch","volume":"196","author":"Nelson","year":"2021","journal-title":"Progress in Neurobiology"},{"key":"bib52","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2204515119","article-title":"Spinal neuropeptide Y Y1 receptor-expressing neurons are a pharmacotherapeutic target for the alleviation of neuropathic pain","volume":"119","author":"Nelson","year":"2022","journal-title":"PNAS"},{"key":"bib53","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1038\/nature13186","article-title":"A mesoscale connectome of the mouse brain","volume":"508","author":"Oh","year":"2014","journal-title":"Nature"},{"key":"bib54","doi-asserted-by":"publisher","first-page":"1135","DOI":"10.1016\/j.neuron.2019.06.016","article-title":"Identification of a spinal circuit for mechanical and persistent spontaneous itch","volume":"103","author":"Pan","year":"2019","journal-title":"Neuron"},{"key":"bib55","doi-asserted-by":"publisher","first-page":"578","DOI":"10.1126\/science.aaf8933","article-title":"Neural circuits for pain: recent advances and current views","volume":"354","author":"Peirs","year":"2016","journal-title":"Science"},{"key":"bib56","doi-asserted-by":"publisher","first-page":"1246","DOI":"10.1016\/j.celrep.2015.09.080","article-title":"Dorsal horn parvalbumin neurons are gate-keepers of touch-evoked pain after nerve injury","volume":"13","author":"Petitjean","year":"2015","journal-title":"Cell Reports"},{"key":"bib57","doi-asserted-by":"publisher","first-page":"1142","DOI":"10.1038\/nature07709","article-title":"The subcellular organization of neocortical excitatory connections","volume":"457","author":"Petreanu","year":"2009","journal-title":"Nature"},{"key":"bib58","doi-asserted-by":"publisher","first-page":"2637","DOI":"10.1523\/JNEUROSCI.19-07-02637.1999","article-title":"Gabaergic neurons that contain Neuropeptide Y selectively target cells with the Neurokinin 1 receptor in Laminae III and IV of the rat spinal cord","volume":"19","author":"Polg\u00e1r","year":"1999","journal-title":"The Journal of Neuroscience"},{"key":"bib59","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1016\/j.pain.2004.06.011","article-title":"Lack of evidence for significant neuronal loss in laminae I-III of the spinal dorsal horn of the rat in the chronic constriction injury model","volume":"111","author":"Polg\u00e1r","year":"2004","journal-title":"Pain"},{"key":"bib60","doi-asserted-by":"publisher","first-page":"1007","DOI":"10.1002\/cne.22570","article-title":"Quantitative study of NPY-expressing GABAergic neurons and axons in rat spinal dorsal horn","volume":"519","author":"Polg\u00e1r","year":"2011","journal-title":"The Journal of Comparative Neurology"},{"key":"bib61","doi-asserted-by":"publisher","first-page":"2606","DOI":"10.1016\/j.pain.2013.05.001","article-title":"Functional differences between neurochemically defined populations of inhibitory interneurons in the rat spinal dorsal horn","volume":"154","author":"Polg\u00e1r","year":"2013","journal-title":"Pain"},{"key":"bib62","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1097\/j.pain.0000000000002677","article-title":"Grpr expression defines a population of superficial dorsal horn vertical cells that have a role in both itch and pain","volume":"164","author":"Polg\u00e1r","year":"2023","journal-title":"Pain"},{"key":"bib63","doi-asserted-by":"publisher","first-page":"886","DOI":"10.1016\/j.neuron.2010.02.025","article-title":"Loss of inhibitory interneurons in the dorsal spinal cord and elevated itch in Bhlhb5 mutant mice","volume":"65","author":"Ross","year":"2010","journal-title":"Neuron"},{"key":"bib64","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1016\/0306-4522(93)90218-5","article-title":"Evidence that neuropeptide Y is present in GABAergic neurons in the superficial dorsal horn of the rat spinal cord","volume":"53","author":"Rowan","year":"1993","journal-title":"Neuroscience"},{"key":"bib65","doi-asserted-by":"publisher","first-page":"2216","DOI":"10.1016\/j.celrep.2018.02.003","article-title":"Massively parallel single nucleus transcriptional profiling defines spinal cord neurons and their activity during behavior","volume":"22","author":"Sathyamurthy","year":"2018","journal-title":"Cell Reports"},{"key":"bib66","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1152\/jn.1994.72.1.169","article-title":"The contribution of GABAA and glycine receptors to central sensitization: disinhibition and touch-evoked allodynia in the spinal cord","volume":"72","author":"Sivilotti","year":"1994","journal-title":"Journal of Neurophysiology"},{"key":"bib67","doi-asserted-by":"publisher","first-page":"4319","DOI":"10.1113\/JP270855","article-title":"Functional heterogeneity of calretinin-expressing neurons in the mouse superficial dorsal horn: implications for spinal pain processing","volume":"593","author":"Smith","year":"2015","journal-title":"The Journal of Physiology"},{"key":"bib68","doi-asserted-by":"publisher","first-page":"7224","DOI":"10.1073\/pnas.1017719108","article-title":"Tonic inhibition of chronic pain by neuropeptide Y","volume":"108","author":"Solway","year":"2011","journal-title":"PNAS"},{"key":"bib69","doi-asserted-by":"publisher","first-page":"700","DOI":"10.1038\/nature06029","article-title":"A gastrin-releasing peptide receptor mediates the itch sensation in the spinal cord","volume":"448","author":"Sun","year":"2007","journal-title":"Nature"},{"key":"bib70","doi-asserted-by":"publisher","first-page":"1531","DOI":"10.1126\/science.1174868","article-title":"Cellular basis of itch sensation","volume":"325","author":"Sun","year":"2009","journal-title":"Science"},{"key":"bib71","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2021220118","article-title":"A subset of spinal dorsal horn interneurons crucial for gating touch-evoked pain-like behavior","volume":"118","author":"Tashima","year":"2021","journal-title":"PNAS"},{"key":"bib72","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1007\/978-1-4939-1096-0","volume-title":"Patch-Clamp Methods and Protocols","author":"Ting","year":"2014"},{"key":"bib73","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1038\/nrn2947","article-title":"Neuronal circuitry for pain processing in the dorsal horn","volume":"11","author":"Todd","year":"2010","journal-title":"Nature Reviews. Neuroscience"},{"key":"bib74","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1016\/0304-3940(91)90093-9","article-title":"Increased neuropeptide Y (NPY)-like immunoreactivity in rat sensory neurons following peripheral axotomy","volume":"124","author":"Wakisaka","year":"1991","journal-title":"Neuroscience Letters"},{"key":"bib75","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1016\/0006-8993(92)90206-o","article-title":"Effects of peripheral nerve injuries and tissue inflammation on the levels of neuropeptide Y-like immunoreactivity in rat primary afferent neurons","volume":"598","author":"Wakisaka","year":"1992","journal-title":"Brain Research"},{"key":"bib76","doi-asserted-by":"publisher","DOI":"10.1101\/2022.03.25.485808","article-title":"A cellular taxonomy of the adult human spinal cord","volume-title":"bioRxiv","author":"Yadav","year":"2022"},{"key":"bib77","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1016\/0304-3959(89)90160-7","article-title":"Behavioral and autonomic correlates of the tactile evoked allodynia produced by spinal glycine inhibition: effects of modulatory receptor systems and excitatory amino acid antagonists","volume":"37","author":"Yaksh","year":"1989","journal-title":"Pain"},{"key":"bib78","doi-asserted-by":"publisher","first-page":"11738","DOI":"10.1073\/pnas.91.24.11738","article-title":"Localization of neuropeptide Y Y1 receptors in the rat nervous system with special reference to somatic receptors on small dorsal root ganglion neurons","volume":"91","author":"Zhang","year":"1994","journal-title":"PNAS"}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/86633\/elife-86633-v1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/86633\/elife-86633-v1.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/86633","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T19:06:55Z","timestamp":1697224015000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/86633"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,25]]},"references-count":78,"alternative-id":["10.7554\/eLife.86633"],"URL":"https:\/\/doi.org\/10.7554\/elife.86633","archive":["CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.7554\/eLife.86633.2","asserted-by":"subject"},{"id-type":"doi","id":"10.1101\/2023.02.10.528013","asserted-by":"subject"},{"id-type":"doi","id":"10.1101\/2023.02.10.528013","asserted-by":"object"}],"is-same-as":[{"id-type":"doi","id":"10.7554\/eLife.86633.3","asserted-by":"subject"}],"is-version-of":[{"id-type":"doi","id":"10.7554\/eLife.86633.1","asserted-by":"subject"},{"id-type":"doi","id":"10.7554\/eLife.86633.2","asserted-by":"subject"}],"is-supplemented-by":[{"id-type":"doi","id":"10.5525\/gla.researchdata.1448","asserted-by":"subject"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,25]]},"assertion":[{"value":"single anonymised","name":"peer_review_transparency","label":"Peer review transparency","group":{"name":"peer_review_taxonomy"}},{"value":"other reviewer(s), editor","name":"peer_review_interaction","label":"Peer review interaction","group":{"name":"peer_review_taxonomy"}},{"value":"review summaries, review reports, author\/editor communication, reviewer identities reviewer opt in, editor identities","name":"peer_review_published","label":"Peer review published","group":{"name":"peer_review_taxonomy"}},{"value":"open (sign in with ORCID iD required)","name":"post_publication_commenting","label":"Post publication commenting","group":{"name":"post_publication_commenting"}}],"article-number":"RP86633"}}