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Here, we evaluate the impact of aging on the profile and activity of adipocyte stem and progenitor cells (ASPCs) and adipocytes during the beiging process in mice. We found that aging increases the expression of\n                    <jats:italic>Cd9<\/jats:italic>\n                    and other fibro-inflammatory genes in fibroblastic ASPCs and blocks their differentiation into beige adipocytes. Fibroblastic ASPC populations from young and aged mice were equally competent for beige differentiation in vitro, suggesting that environmental factors suppress adipogenesis in vivo. Examination of adipocytes by single nucleus RNA-sequencing identified compositional and transcriptional differences in adipocyte populations with aging and cold exposure. Notably, cold exposure induced an adipocyte population expressing high levels of de novo lipogenesis (DNL) genes, and this response was severely blunted in aged animals. We further identified\n                    <jats:italic>Npr3<\/jats:italic>\n                    , which encodes the natriuretic peptide clearance receptor, as a marker gene for a subset of white adipocytes and an aging-upregulated gene in adipocytes. In summary, this study indicates that aging blocks beige adipogenesis and dysregulates adipocyte responses to cold exposure and provides a resource for identifying cold and aging-regulated pathways in adipose tissue.\n                  <\/jats:p>","DOI":"10.7554\/elife.87756","type":"journal-article","created":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T10:43:26Z","timestamp":1685529806000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.87756","source":"Crossref","is-referenced-by-count":38,"title":["Aging impairs cold-induced beige adipogenesis and adipocyte metabolic reprogramming"],"prefix":"10.7554","volume":"12","author":[{"given":"Corey D","family":"Holman","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00b30xv10","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Diabetes, Obesity & Metabolism, Perelman School of Medicine at the University of Pennsylvania","place":["Philadelphia, United States"]},{"name":"Department of Cell and Developmental Biology; 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