{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T05:51:40Z","timestamp":1791438700160,"version":"4.3.3"},"reference-count":99,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2023,12,18]],"date-time":"2023-12-18T00:00:00Z","timestamp":1702857600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,12,18]],"date-time":"2023-12-18T00:00:00Z","timestamp":1702857600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,12,18]],"date-time":"2023-12-18T00:00:00Z","timestamp":1702857600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"crossref","award":["10.58275\/aha.23post1011626.pc.gr.161347"],"award-info":[{"award-number":["10.58275\/aha.23post1011626.pc.gr.161347"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01 AI145941"],"award-info":[{"award-number":["R01 AI145941"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["F31 AI172110"],"award-info":[{"award-number":["F31 AI172110"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000925","name":"NHMRC","doi-asserted-by":"crossref","award":["GNT2020822"],"award-info":[{"award-number":["GNT2020822"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009727","name":"Hospital Research Foundation","doi-asserted-by":"crossref","award":["C-MCF-52-2019"],"award-info":[{"award-number":["C-MCF-52-2019"]}],"id":[{"id":"10.13039\/100009727","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Apicomplexan parasites exhibit tremendous diversity in much of their fundamental cell biology, but study of these organisms using light microscopy is often hindered by their small size. Ultrastructural expansion microscopy (U-ExM) is a microscopy preparation method that physically expands the sample by ~4.5\u00d7. Here, we apply U-ExM to the human malaria parasite\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    during the asexual blood stage of its lifecycle to understand how this parasite is organized in three dimensions. Using a combination of dye-conjugated reagents and immunostaining, we have cataloged 13 different\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    structures or organelles across the intraerythrocytic development of this parasite and made multiple observations about fundamental parasite cell biology. We describe that the outer centriolar plaque and its associated proteins anchor the nucleus to the parasite plasma membrane during mitosis. Furthermore, the rhoptries, Golgi, basal complex, and inner membrane complex, which form around this anchoring site while nuclei are still dividing, are concurrently segregated and maintain an association to the outer centriolar plaque until the start of segmentation. We also show that the mitochondrion and apicoplast undergo sequential fission events while maintaining an association with the outer centriolar plaque during cytokinesis. Collectively, this study represents the most detailed ultrastructural analysis of\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    during its intraerythrocytic development to date and sheds light on multiple poorly understood aspects of its organelle biogenesis and fundamental cell biology.\n                  <\/jats:p>","DOI":"10.7554\/elife.88088","type":"journal-article","created":{"date-parts":[[2023,5,23]],"date-time":"2023-05-23T15:07:14Z","timestamp":1684854434000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.88088","source":"Crossref","is-referenced-by-count":48,"title":["Atlas of Plasmodium falciparum intraerythrocytic development using expansion microscopy"],"prefix":"10.7554","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1573-6139","authenticated-orcid":true,"given":"Benjamin","family":"Liffner","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Indiana University School of Medicine","place":["Indianapolis, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2033-461X","authenticated-orcid":true,"given":"Ana Karla","family":"Cepeda Diaz","sequence":"first","affiliation":[{"name":"Biological and Biomedical Sciences, Harvard Medical School","place":["Boston, United States"]},{"id":[{"id":"https:\/\/ror.org\/00dvg7y05","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Infectious Diseases, Boston Children\u2019s Hospital","place":["Boston, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7639-2800","authenticated-orcid":true,"given":"James","family":"Blauwkamp","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Indiana University School of Medicine","place":["Indianapolis, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Anaguano","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00te3t702","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Tropical and Emerging Global Diseases, University of Georgia","place":["Athens, United States"]},{"id":[{"id":"https:\/\/ror.org\/00te3t702","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Cellular Biology, Franklin College of Arts and Sciences, University of Georgia","place":["Athens, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8673-3437","authenticated-orcid":true,"given":"Sonja","family":"Frolich","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00892tw58","id-type":"ROR","asserted-by":"publisher"}],"name":"Research Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide","place":["Adelaide, Australia"]},{"id":[{"id":"https:\/\/ror.org\/00892tw58","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Photonics and Advanced Sensing, University of Adelaide","place":["Adelaide, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6367-6284","authenticated-orcid":true,"given":"Vasant","family":"Muralidharan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00te3t702","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Tropical and Emerging Global Diseases, University of Georgia","place":["Athens, United States"]},{"id":[{"id":"https:\/\/ror.org\/00te3t702","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Cellular Biology, Franklin College of Arts and Sciences, University of Georgia","place":["Athens, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5073-1405","authenticated-orcid":true,"given":"Danny W","family":"Wilson","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00892tw58","id-type":"ROR","asserted-by":"publisher"}],"name":"Research Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide","place":["Adelaide, Australia"]},{"id":[{"id":"https:\/\/ror.org\/00892tw58","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Photonics and Advanced Sensing, University of Adelaide","place":["Adelaide, Australia"]},{"id":[{"id":"https:\/\/ror.org\/05ktbsm52","id-type":"ROR","asserted-by":"publisher"}],"name":"Burnet Institute, 85 Commercial Road","place":["Melbourne, Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5883-7271","authenticated-orcid":true,"given":"Jeffrey D","family":"Dvorin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00dvg7y05","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Infectious Diseases, Boston Children\u2019s Hospital","place":["Boston, United States"]},{"id":[{"id":"https:\/\/ror.org\/03vek6s52","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pediatrics, Harvard Medical School","place":["Boston, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2468-8156","authenticated-orcid":true,"given":"Sabrina","family":"Absalon","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Indiana University School of Medicine","place":["Indianapolis, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2023,12,18]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00130-18","article-title":"Calcium-Dependent Protein Kinase 5 Is Required for Release of Egress-Specific Organelles in Plasmodium falciparum","volume":"9","author":"Absalon","year":"2018","journal-title":"mBio"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1083\/jcb.35.1.103","article-title":"Ultrastructure of the pellicular complex of Plasmodium fallax","volume":"35","author":"Aikawa","year":"1967","journal-title":"The Journal of Cell Biology"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1016\/0014-4894(71)90094-4","article-title":"Parasitological review: Plasmodium: the fine structure of malarial parasites","volume":"30","author":"Aikawa","year":"1971","journal-title":"Experimental Parasitology"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/B978-0-12-394309-5.00001-8","article-title":"Cytoskeleton assembly in Toxoplasma gondii cell division","volume":"298","author":"Anderson-White","year":"2012","journal-title":"International Review of Cell and Molecular Biology"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1017\/s0031182099006320","article-title":"Ultrastructure of rhoptry development in Plasmodium falciparum erythrocytic schizonts","volume":"121 (Pt 3)","author":"Bannister","year":"2000","journal-title":"Parasitology"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"752","DOI":"10.1016\/j.chom.2020.09.011","article-title":"A Comprehensive Subcellular Atlas of the Toxoplasma Proteome via hyperLOPIT Provides Spatial Context for Protein Functions","volume":"28","author":"Barylyuk","year":"2020","journal-title":"Cell Host & Microbe"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1111\/mmi.14674","article-title":"Biogenesis and discharge of the rhoptries: Key organelles for entry and hijack of host cells by the Apicomplexa","volume":"115","author":"Ben Chaabene","year":"2021","journal-title":"Molecular Microbiology"},{"key":"bib8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3001020","article-title":"Expansion microscopy provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid","volume":"19","author":"Bertiaux","year":"2021","journal-title":"PLOS Biology"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"450","DOI":"10.1038\/nmeth.4223","article-title":"A genetic system to study Plasmodium falciparum protein function","volume":"14","author":"Birnbaum","year":"2017","journal-title":"Nature Methods"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1084\/jem.180.1.389","article-title":"Antibodies inhibit the protease-mediated processing of a malaria merozoite surface protein","volume":"180","author":"Blackman","year":"1994","journal-title":"The Journal of Experimental Medicine"},{"key":"bib11","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01507-23","article-title":"Essential function of alveolin PfIMC1g in the Plasmodium falciparum asexual blood stage","volume":"14","author":"Cepeda Diaz","year":"2023","journal-title":"mBio"},{"key":"bib12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003344","article-title":"Malaria parasite cGMP-dependent protein kinase regulates blood stage merozoite secretory organelle discharge and egress","volume":"9","author":"Collins","year":"2013","journal-title":"PLOS Pathogens"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"2032","DOI":"10.1016\/j.bbagen.2014.02.009","article-title":"Malarial hemozoin: from target to tool","volume":"1840","author":"Coronado","year":"2014","journal-title":"Biochimica et Biophysica Acta"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1016\/j.pt.2013.03.003","article-title":"Plasmodium rhoptry proteins: why order is important","volume":"29","author":"Counihan","year":"2013","journal-title":"Trends in Parasitology"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1038\/nature02825","article-title":"Centrosomes direct cell polarity independently of microtubule assembly in C. elegans embryos","volume":"431","author":"Cowan","year":"2004","journal-title":"Nature"},{"key":"bib16","doi-asserted-by":"publisher","DOI":"10.3390\/cells11101709","article-title":"The Dually Localized EF-Hand Domain-Containing Protein TgEFP1 Regulates the Lytic Cycle of Toxoplasma gondii","volume":"11","author":"Dave","year":"2022","journal-title":"Cells"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"704","DOI":"10.1038\/nature03811","article-title":"Centrosome localization determines neuronal polarity","volume":"436","author":"de Anda","year":"2005","journal-title":"Nature"},{"key":"bib18","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.13018","article-title":"Biogenesis and secretion of micronemes in Toxoplasma gondii","volume":"21","author":"Dubois","year":"2019","journal-title":"Cellular Microbiology"},{"key":"bib19","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201847102","article-title":"A pan-apicomplexan phosphoinositide-binding protein acts in malarial microneme exocytosis","volume":"20","author":"Ebrahimzadeh","year":"2019","journal-title":"EMBO Reports"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"4763","DOI":"10.1002\/j.1460-2075.1993.tb06165.x","article-title":"Identification and localization of ERD2 in the malaria parasite Plasmodium falciparum: separation from sites of sphingomyelin synthesis and implications for organization of the Golgi","volume":"12","author":"Elmendorf","year":"1993","journal-title":"The EMBO Journal"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/j.abb.2003.12.031","article-title":"Discrimination of depolarized from polarized mitochondria by confocal fluorescence resonance energy transfer","volume":"422","author":"Elmore","year":"2004","journal-title":"Archives of Biochemistry and Biophysics"},{"key":"bib22","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-32151-0","article-title":"Proteomic characterization of the Toxoplasma gondii cytokinesis machinery portrays an expanded hierarchy of its assembly and function","volume":"13","author":"Engelberg","year":"2022","journal-title":"Nature Communications"},{"key":"bib23","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-23919-x","article-title":"Composition and stage dynamics of mitochondrial complexes in Plasmodium falciparum","volume":"12","author":"Evers","year":"2021","journal-title":"Nature Communications"},{"key":"bib24","doi-asserted-by":"publisher","DOI":"10.1101\/2023.03.10.531920","article-title":"Comparative 3D Ultrastructure of Plasmodium falciparum Gametocytes","volume-title":"bioRxiv","author":"Evers","year":"2023"},{"key":"bib25","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.611801","article-title":"The dynamic roles of the inner membrane complex in the multiple stages of the malaria parasite","volume":"10","author":"Ferreira","year":"2020","journal-title":"Frontiers in Cellular and Infection Microbiology"},{"key":"bib26","doi-asserted-by":"publisher","first-page":"13719","DOI":"10.1073\/pnas.1919501117","article-title":"Plastid biogenesis in malaria parasites requires the interactions and catalytic activity of the Clp proteolytic system","volume":"117","author":"Florentin","year":"2020","journal-title":"PNAS"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1038\/nrmicro3184","article-title":"Cell division in apicomplexan parasites","volume":"12","author":"Francia","year":"2014","journal-title":"Nature Reviews. Microbiology"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1146\/annurev.micro.51.1.97","article-title":"Hemoglobin metabolism in the malaria parasite Plasmodium falciparum","volume":"51","author":"Francis","year":"1997","journal-title":"Annual Review of Microbiology"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1038\/s41592-018-0238-1","article-title":"Imaging cellular ultrastructures using expansion microscopy (U-ExM)","volume":"16","author":"Gambarotto","year":"2019","journal-title":"Nature Methods"},{"key":"bib30","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-23626-7","article-title":"4D analysis of malaria parasite invasion offers insights into erythrocyte membrane remodeling and parasitophorous vacuole formation","volume":"12","author":"Geoghegan","year":"2021","journal-title":"Nature Communications"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"474","DOI":"10.1128\/EC.00314-10","article-title":"Mitosis in the human malaria parasite Plasmodium falciparum","volume":"10","author":"Gerald","year":"2011","journal-title":"Eukaryotic Cell"},{"key":"bib32","doi-asserted-by":"publisher","DOI":"10.1111\/jeu.12904","article-title":"Dense granule biogenesis, secretion, and function in Toxoplasma gondii","volume":"69","author":"Griffith","year":"2022","journal-title":"The Journal of Eukaryotic Microbiology"},{"key":"bib33","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1169","article-title":"Development and host cell modifications of Plasmodium falciparum blood stages in four dimensions","volume":"2","author":"Gr\u00fcring","year":"2011","journal-title":"Nature Communications"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"15913","DOI":"10.1074\/jbc.M108393200","article-title":"Purification and molecular characterization of cGMP-dependent protein kinase from Apicomplexan parasites: a novel chemotherapeutic target","volume":"277","author":"Gurnett","year":"2002","journal-title":"The Journal of Biological Chemistry"},{"key":"bib35","doi-asserted-by":"publisher","first-page":"3439","DOI":"10.1073\/pnas.1619441114","article-title":"Parasitophorous vacuole poration precedes its rupture and rapid host erythrocyte cytoskeleton collapse in Plasmodium falciparum egress","volume":"114","author":"Hale","year":"2017","journal-title":"PNAS"},{"key":"bib36","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1016\/0166-6851(83)90025-7","article-title":"Antigens of the erythrocytes stages of the human malaria parasite Plasmodium falciparum detected by monoclonal antibodies","volume":"7","author":"Hall","year":"1983","journal-title":"Molecular and Biochemical Parasitology"},{"key":"bib37","doi-asserted-by":"publisher","first-page":"1457","DOI":"10.1111\/cmi.12132","article-title":"Electron tomography of Plasmodium falciparum merozoites reveals core cellular events that underpin erythrocyte invasion","volume":"15","author":"Hanssen","year":"2013","journal-title":"Cellular Microbiology"},{"key":"bib38","doi-asserted-by":"publisher","first-page":"632","DOI":"10.1111\/cmi.12285","article-title":"The inner membrane complex through development of Toxoplasma gondii and Plasmodium","volume":"16","author":"Harding","year":"2014","journal-title":"Cellular Microbiology"},{"key":"bib39","doi-asserted-by":"publisher","first-page":"5751","DOI":"10.1128\/IAI.70.10.5751-5758.2002","article-title":"Independent translocation of two micronemal proteins in developing Plasmodium falciparum merozoites","volume":"70","author":"Healer","year":"2002","journal-title":"Infection and Immunity"},{"key":"bib40","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0040010","article-title":"Organizational changes of the daughter basal complex during the parasite replication of Toxoplasma gondii","volume":"4","author":"Hu","year":"2008","journal-title":"PLOS Pathogens"},{"key":"bib41","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1038\/nbt.1597","article-title":"Transcriptional profiling of growth perturbations of the human malaria parasite Plasmodium falciparum","volume":"28","author":"Hu","year":"2010","journal-title":"Nature Biotechnology"},{"key":"bib42","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2012.00235","article-title":"Microtubule-organizing center polarity and the immunological synapse: protein kinase C and beyond","volume":"3","author":"Huse","year":"2012","journal-title":"Frontiers in Immunology"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"74","DOI":"10.1016\/j.molbiopara.2006.01.009","article-title":"Plasmodium falciparum erythrocyte invasion: A conserved myosin associated complex","volume":"147","author":"Jones","year":"2006","journal-title":"Molecular and Biochemical Parasitology"},{"key":"bib44","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/j.molbiopara.2009.06.007","article-title":"Effects of calcium signaling on Plasmodium falciparum erythrocyte invasion and post-translational modification of gliding-associated protein 45 (PfGAP45)","volume":"168","author":"Jones","year":"2009","journal-title":"Molecular and Biochemical Parasitology"},{"key":"bib45","doi-asserted-by":"publisher","first-page":"1803","DOI":"10.1364\/BOE.2.001803","article-title":"Effect of the size and shape of a red blood cell on elastic light scattering properties at the single-cell level","volume":"2","author":"Kinnunen","year":"2011","journal-title":"Biomedical Optics Express"},{"key":"bib46","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abj5362","article-title":"Asynchronous nuclear cycles in multinucleated Plasmodium falciparum facilitate rapid proliferation","volume":"8","author":"Klaus","year":"2022","journal-title":"Science Advances"},{"key":"bib47","doi-asserted-by":"publisher","first-page":"2113","DOI":"10.1093\/molbev\/mss081","article-title":"Evolution and architecture of the inner membrane complex in asexual and sexual stages of the malaria parasite","volume":"29","author":"Kono","year":"2012","journal-title":"Molecular Biology and Evolution"},{"key":"bib48","doi-asserted-by":"publisher","DOI":"10.1101\/2022.06.02.494549","article-title":"Stable and Ancient Endocytic Structures Navigate the Complex Pellicle of Apicomplexan Parasites","volume-title":"bioRxiv","author":"Koreny","year":"2022"},{"key":"bib49","doi-asserted-by":"publisher","first-page":"1757","DOI":"10.1111\/j.1462-5822.2012.01836.x","article-title":"Structural basis for chirality and directional motility of Plasmodium sporozoites","volume":"14","author":"Kudryashev","year":"2012","journal-title":"Cellular Microbiology"},{"key":"bib50","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/0166-6851(91)90163-z","article-title":"Induction and localization of Plasmodium falciparum stress proteins related to the heat shock protein 70 family","volume":"48","author":"Kumar","year":"1991","journal-title":"Molecular and Biochemical Parasitology"},{"key":"bib51","doi-asserted-by":"crossref","first-page":"418","DOI":"10.2307\/3280287","article-title":"Synchronization of Plasmodium falciparum erythrocytic stages in culture","volume":"65","author":"Lambros","year":"1979","journal-title":"The Journal of Parasitology"},{"key":"bib52","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-32579-4","article-title":"Repurposing the mitotic machinery to drive cellular elongation and chromatin reorganisation in Plasmodium falciparum gametocytes","volume":"13","author":"Li","year":"2022","journal-title":"Nature Communications"},{"key":"bib53","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9112306","article-title":"Expansion Microscopy Reveals Plasmodium falciparum Blood-Stage Parasites Undergo Anaphase with A Chromatin Bridge in the Absence of Mini-Chromosome Maintenance Complex Binding Protein","volume":"9","author":"Liffner","year":"2021","journal-title":"Microorganisms"},{"key":"bib54","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1016\/j.pt.2022.03.007","article-title":"Hand-in-hand advances in microscopy and Plasmodium nuclear biology","volume":"38","author":"Liffner","year":"2022","journal-title":"Trends in Parasitology"},{"key":"bib55","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-022-03020-9","article-title":"Cell biological analysis reveals an essential role for Pfcerli2 in erythrocyte invasion by malaria parasites","volume":"5","author":"Liffner","year":"2022","journal-title":"Communications Biology"},{"key":"bib56","doi-asserted-by":"publisher","DOI":"10.1101\/2022.11.14.516383","article-title":"Nanoscopy of Organelles and Tissues with Iterative Ultrastructure Expansion Microscopy (iU-ExM)","volume-title":"bioRxiv","author":"Louvel","year":"2022"},{"key":"bib57","doi-asserted-by":"publisher","DOI":"10.1101\/2022.08.31.505163","article-title":"Plasmodium falciparum CRK4 Links Early Mitotic Events to the Onset of S-Phase during Schizogony","volume-title":"bioRxiv","author":"Machado","year":"2022"},{"key":"bib58","doi-asserted-by":"publisher","first-page":"31871","DOI":"10.1074\/jbc.M800028200","article-title":"Centrins, cell cycle regulation proteins in human malaria parasite Plasmodium falciparum","volume":"283","author":"Mahajan","year":"2008","journal-title":"The Journal of Biological Chemistry"},{"key":"bib59","doi-asserted-by":"publisher","first-page":"819","DOI":"10.1007\/s00418-008-0509-5","article-title":"Use of Bodipy-labeled sphingolipid and cholesterol analogs to examine membrane microdomains in cells","volume":"130","author":"Marks","year":"2008","journal-title":"Histochemistry and Cell Biology"},{"key":"bib60","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1128\/EC.00267-14","article-title":"Defining the morphology and mechanism of the hemoglobin transport pathway in Plasmodium falciparum-infected erythrocytes","volume":"14","author":"Milani","year":"2015","journal-title":"Eukaryotic Cell"},{"key":"bib61","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.656976","article-title":"The ringleaders: Understanding the apicomplexan basal complex through comparison to established contractile ring systems","volume":"11","author":"Morano","year":"2021","journal-title":"Frontiers in Cellular and Infection Microbiology"},{"key":"bib62","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1128\/MMBR.66.1.21-38.2002","article-title":"Cytoskeleton of apicomplexan parasites","volume":"66","author":"Morrissette","year":"2002","journal-title":"Microbiology and Molecular Biology Reviews"},{"key":"bib63","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/B978-0-12-420070-8.00008-8","article-title":"Labeling a protein with fluorophores using NHS ester derivitization","volume":"536","author":"Nanda","year":"2014","journal-title":"Methods in Enzymology"},{"key":"bib64","doi-asserted-by":"publisher","first-page":"1559","DOI":"10.1242\/jcs.021089","article-title":"Organellar dynamics during the cell cycle of Toxoplasma gondii","volume":"121","author":"Nishi","year":"2008","journal-title":"Journal of Cell Science"},{"key":"bib65","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.260083","article-title":"IMC10 and LMF1 mediate mitochondrial morphology through mitochondrion-pellicle contact sites in Toxoplasma gondii","volume":"135","author":"Oliveira Souza","year":"2022","journal-title":"Journal of Cell Science"},{"key":"bib66","doi-asserted-by":"publisher","first-page":"1363","DOI":"10.1177\/44.12.8985128","article-title":"Analysis of mitochondrial morphology and function with novel fixable fluorescent stains","volume":"44","author":"Poot","year":"1996","journal-title":"The Journal of Histochemistry and Cytochemistry"},{"key":"bib67","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-35270-w","article-title":"Apical anchorage and stabilization of subpellicular microtubules by apical polar ring ensures Plasmodium ookinete infection in mosquito","volume":"13","author":"Qian","year":"2022","journal-title":"Nature Communications"},{"key":"bib68","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1010223","article-title":"Expansion microscopy of Plasmodium gametocytes reveals the molecular architecture of a bipartite microtubule organisation centre coordinating mitosis with axoneme assembly","volume":"18","author":"Rashpa","year":"2022","journal-title":"PLOS Pathogens"},{"key":"bib69","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-36999-8","article-title":"The Skp1-Cullin1-FBXO1 complex is a pleiotropic regulator required for the formation of gametes and motile forms in Plasmodium berghei","volume":"14","author":"Rashpa","year":"2023","journal-title":"Nature Communications"},{"key":"bib70","doi-asserted-by":"publisher","first-page":"14815","DOI":"10.1074\/jbc.M109.080770","article-title":"Interaction between Plasmodium falciparum apical membrane antigen 1 and the rhoptry neck protein complex defines a key step in the erythrocyte invasion process of malaria parasites","volume":"285","author":"Richard","year":"2010","journal-title":"Journal of Biological Chemistry"},{"key":"bib71","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.chom.2010.12.003","article-title":"Super-resolution dissection of coordinated events during malaria parasite invasion of the human erythrocyte","volume":"9","author":"Riglar","year":"2011","journal-title":"Cell Host & Microbe"},{"key":"bib72","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.1010038","article-title":"Identification of new components of the basal pole of Toxoplasma gondii provides novel insights into its molecular organization and functions","volume":"12","author":"Roum\u00e9gous","year":"2022","journal-title":"Frontiers in Cellular and Infection Microbiology"},{"key":"bib73","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10214-z","article-title":"An essential contractile ring protein controls cell division in Plasmodium falciparum","volume":"10","author":"Rudlaff","year":"2019","journal-title":"Nature Communications"},{"key":"bib74","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008587","article-title":"Three-dimensional ultrastructure of Plasmodium falciparum throughout cytokinesis","volume":"16","author":"Rudlaff","year":"2020","journal-title":"PLOS Pathogens"},{"key":"bib75","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1073\/pnas.98.1.271","article-title":"Malaria parasite exit from the host erythrocyte: A two-step process requiring extraerythrocytic proteolysis","volume":"98","author":"Salmon","year":"2001","journal-title":"PNAS"},{"key":"bib76","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/j.ceb.2016.09.003","article-title":"Microtubule-organizing centers: from the centrosome to non-centrosomal sites","volume":"44","author":"Sanchez","year":"2017","journal-title":"Current Opinion in Cell Biology"},{"key":"bib77","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00403-22","article-title":"Previously Unidentified Histone H1-Like Protein Is Involved in Cell Division and Ribosome Biosynthesis in Toxoplasma gondii","volume":"7","author":"Severo","year":"2022","journal-title":"mSphere"},{"key":"bib78","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002392","article-title":"A systematic screen to discover and analyze apicoplast proteins identifies A conserved and essential protein import factor","volume":"7","author":"Sheiner","year":"2011","journal-title":"PLOS Pathogens"},{"key":"bib79","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0018393","article-title":"Delineation of stage specific expression of Plasmodium falciparum EBA-175 by biologically functional region II monoclonal antibodies","volume":"6","author":"Sim","year":"2011","journal-title":"PLOS ONE"},{"key":"bib80","doi-asserted-by":"publisher","DOI":"10.26508\/lsa.202101199","article-title":"An extended DNA-free intranuclear compartment organizes centrosome microtubules in malaria parasites","volume":"4","author":"Simon","year":"2021","journal-title":"Life Science Alliance"},{"key":"bib81","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1016\/j.chom.2012.03.006","article-title":"Toxoplasma sortilin-like receptor regulates protein transport and is essential for apical secretory organelle biogenesis and host infection","volume":"11","author":"Sloves","year":"2012","journal-title":"Cell Host & Microbe"},{"key":"bib82","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1128\/EC.00186-09","article-title":"The malaria parasite cyclic GMP-dependent protein kinase plays a central role in blood-stage schizogony","volume":"9","author":"Taylor","year":"2010","journal-title":"Eukaryotic Cell"},{"key":"bib83","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01859-22","article-title":"Separate To Operate: the Centriole-Free Inner Core of the Centrosome Regulates the Assembly of the Intranuclear Spindle in Toxoplasma gondii","volume":"13","author":"Tomasina","year":"2022","journal-title":"mBio"},{"key":"bib84","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.molbiopara.2004.05.009","article-title":"Localization of organellar proteins in Plasmodium falciparum using a novel set of transfection vectors and a new immunofluorescence fixation method","volume":"137","author":"Tonkin","year":"2004","journal-title":"Molecular and Biochemical Parasitology"},{"key":"bib85","doi-asserted-by":"publisher","first-page":"4648","DOI":"10.1074\/jbc.M307859200","article-title":"Characterization of a membrane-associated rhoptry protein of Plasmodium falciparum","volume":"279","author":"Topolska","year":"2004","journal-title":"The Journal of Biological Chemistry"},{"key":"bib86","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.56635","article-title":"Essential function of the alveolin network in the subpellicular microtubules and conoid assembly in Toxoplasma gondii","volume":"9","author":"Tosetti","year":"2020","journal-title":"eLife"},{"key":"bib87","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1126\/science.781840","article-title":"Human malaria parasites in continuous culture","volume":"193","author":"Trager","year":"1976","journal-title":"Science"},{"key":"bib88","doi-asserted-by":"publisher","first-page":"586","DOI":"10.1002\/cm.20469","article-title":"RNG1 is a late marker of the apical polar ring in Toxoplasma gondii","volume":"67","author":"Tran","year":"2010","journal-title":"Cytoskeleton"},{"key":"bib89","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1111\/j.1365-2958.2005.04699.x","article-title":"Development of the endoplasmic reticulum, mitochondrion and apicoplast during the asexual life cycle of Plasmodium falciparum","volume":"57","author":"van Dooren","year":"2005","journal-title":"Molecular Microbiology"},{"key":"bib90","doi-asserted-by":"publisher","first-page":"596","DOI":"10.1111\/j.1574-6976.2006.00027.x","article-title":"Metabolic maps and functions of the        Plasmodium        mitochondrion","volume":"30","author":"van Dooren","year":"2006","journal-title":"FEMS Microbiology Reviews"},{"key":"bib91","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00614-21","article-title":"A Prioritized and Validated Resource of Mitochondrial Proteins in Plasmodium Identifies Unique Biology","volume":"6","author":"van Esveld","year":"2021","journal-title":"mSphere"},{"key":"bib92","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01409-21","article-title":"Organelle dynamics in apicomplexan parasites","volume":"12","author":"Verhoef","year":"2021","journal-title":"mBio"},{"key":"bib93","doi-asserted-by":"publisher","first-page":"568","DOI":"10.1038\/nmeth.3365","article-title":"High-performance probes for light and electron microscopy","volume":"12","author":"Viswanathan","year":"2015","journal-title":"Nature Methods"},{"key":"bib94","doi-asserted-by":"publisher","DOI":"10.1101\/2022.07.26.501452","article-title":"Ca2+-inducible phase separation of centrins in proliferating malaria parasites","volume-title":"bioRxiv","author":"Vo\u00df","year":"2022"},{"key":"bib95","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1038\/s41592-018-0219-4","article-title":"Expansion microscopy: principles and uses in biological research","volume":"16","author":"Wassie","year":"2019","journal-title":"Nature Methods"},{"key":"bib96","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004670","article-title":"Revealing the Sequence and Resulting Cellular Morphology of Receptor-Ligand Interactions during Plasmodium falciparum Invasion of Erythrocytes","volume":"11","author":"Weiss","year":"2015","journal-title":"PLOS Pathogens"},{"key":"bib97","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2018101056","article-title":"Individual cristae within the same mitochondrion display different membrane potentials and are functionally independent","volume":"38","author":"Wolf","year":"2019","journal-title":"The EMBO Journal"},{"key":"bib98","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1016\/j.pt.2020.03.006","article-title":"K13, the Cytostome, and Artemisinin Resistance","volume":"36","author":"Xie","year":"2020","journal-title":"Trends in Parasitology"},{"key":"bib99","doi-asserted-by":"publisher","first-page":"2917","DOI":"10.1016\/j.celrep.2019.10.095","article-title":"Decreased K13 Abundance Reduces Hemoglobin Catabolism and Proteotoxic Stress, Underpinning Artemisinin Resistance","volume":"29","author":"Yang","year":"2019","journal-title":"Cell Reports"}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/88088\/elife-88088-v1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/88088\/elife-88088-v1.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/88088","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,18]],"date-time":"2023-12-18T10:45:48Z","timestamp":1702896348000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/88088"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,18]]},"references-count":99,"alternative-id":["10.7554\/eLife.88088"],"URL":"https:\/\/doi.org\/10.7554\/elife.88088","archive":["CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.7554\/eLife.88088.2","asserted-by":"subject"},{"id-type":"doi","id":"10.1101\/2023.03.22.533773","asserted-by":"subject"}],"is-same-as":[{"id-type":"doi","id":"10.7554\/eLife.88088.3","asserted-by":"subject"}],"is-version-of":[{"id-type":"doi","id":"10.7554\/eLife.88088.1","asserted-by":"subject"},{"id-type":"doi","id":"10.7554\/eLife.88088.2","asserted-by":"subject"}],"is-financed-by":[{"id-type":"doi","id":"10.58275\/aha.23post1011626.pc.gr.161347","asserted-by":"subject"}],"is-supplemented-by":[{"id-type":"doi","id":"10.5061\/dryad.9s4mw6mp4","asserted-by":"subject"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,18]]},"assertion":[{"value":"single anonymised","name":"peer_review_transparency","label":"Peer review transparency","group":{"name":"peer_review_taxonomy"}},{"value":"other reviewer(s), editor","name":"peer_review_interaction","label":"Peer review interaction","group":{"name":"peer_review_taxonomy"}},{"value":"review summaries, review reports, author\/editor communication, reviewer identities reviewer opt in, editor identities","name":"peer_review_published","label":"Peer review published","group":{"name":"peer_review_taxonomy"}},{"value":"open (sign in with ORCID iD required)","name":"post_publication_commenting","label":"Post publication commenting","group":{"name":"post_publication_commenting"}}],"article-number":"RP88088"}}