{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:39:30Z","timestamp":1787567970923,"version":"build-2736575974"},"reference-count":48,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T00:00:00Z","timestamp":1734566400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T00:00:00Z","timestamp":1734566400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T00:00:00Z","timestamp":1734566400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82103539"],"award-info":[{"award-number":["82103539"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82074298"],"award-info":[{"award-number":["82074298"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017513","name":"Sichuan Association for Science and Technology","doi-asserted-by":"publisher","award":["2021YFH0005"],"award-info":[{"award-number":["2021YFH0005"]}],"id":[{"id":"10.13039\/100017513","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017513","name":"Sichuan Association for Science and Technology","doi-asserted-by":"publisher","award":["2022YFH0003"],"award-info":[{"award-number":["2022YFH0003"]}],"id":[{"id":"10.13039\/100017513","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020207","name":"Health Commission of Sichuan Province","doi-asserted-by":"publisher","award":["2023104"],"award-info":[{"award-number":["2023104"]}],"id":[{"id":"10.13039\/501100020207","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82173253"],"award-info":[{"award-number":["82173253"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Bestrophin isoform 4 (\n                    <jats:italic>BEST4<\/jats:italic>\n                    ) is a newly identified subtype of the calcium-activated chloride channel family. Analysis of colonic epithelial cell diversity by single-cell RNA-sequencing has revealed the existence of a cluster of\n                    <jats:italic>BEST4<\/jats:italic>\n                    + mature colonocytes in humans. However, if the role of\n                    <jats:italic>BEST4<\/jats:italic>\n                    is involved in regulating tumour progression remains largely unknown. In this study, we demonstrate that\n                    <jats:italic>BEST4<\/jats:italic>\n                    overexpression attenuates cell proliferation, colony formation, and mobility in colorectal cancer (CRC) in vitro, and impedes the tumour growth and the liver metastasis in vivo. BEST4 is co-expressed with hairy\/enhancer of split 4 (\n                    <jats:italic>HES4<\/jats:italic>\n                    ) in the nucleus of cells, and HES4 signals\n                    <jats:italic>BEST4<\/jats:italic>\n                    by interacting with the upstream region of the\n                    <jats:italic>BEST4<\/jats:italic>\n                    promoter.\n                    <jats:italic>BEST4<\/jats:italic>\n                    is epistatic to\n                    <jats:italic>HES4<\/jats:italic>\n                    and downregulates TWIST1, thereby inhibiting epithelial-to-mesenchymal transition (EMT) in CRC. Conversely, knockout of BEST4 using CRISPR\/Cas9 in CRC cells revitalises tumour growth and induces EMT. Furthermore, the low level of the\n                    <jats:italic>BEST4<\/jats:italic>\n                    mRNA is correlated with advanced and the worse prognosis, suggesting its potential role involving CRC progression.\n                  <\/jats:p>","DOI":"10.7554\/elife.88879","type":"journal-article","created":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T13:50:25Z","timestamp":1698846625000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.88879","source":"Crossref","is-referenced-by-count":7,"title":["Bestrophin-4 relays HES4 and interacts with TWIST1 to suppress epithelial-to-mesenchymal transition in colorectal cancer cells"],"prefix":"10.7554","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7201-8360","authenticated-orcid":true,"given":"Zijing","family":"Wang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/011ashp19","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Gastroenterology and Hepatology, Sichuan University-University of Oxford Huaxi Joint Centre for Gastrointestinal Cancer, West China Hospital, Sichuan University","place":["Chengdu, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Bihan","family":"Xia","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/011ashp19","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Gastroenterology and Hepatology, Sichuan University-University of Oxford Huaxi Joint Centre for Gastrointestinal Cancer, West China Hospital, Sichuan University","place":["Chengdu, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"}]},{"given":"Shaochong","family":"Qi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/011ashp19","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Gastroenterology and Hepatology, Sichuan University-University of Oxford Huaxi Joint Centre for Gastrointestinal Cancer, West China Hospital, Sichuan University","place":["Chengdu, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"software","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"given":"Xian","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/011ashp19","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Gastroenterology and Hepatology, Sichuan University-University of Oxford Huaxi Joint Centre for Gastrointestinal Cancer, West China Hospital, Sichuan University","place":["Chengdu, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"}]},{"given":"Xiaoshuang","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/011ashp19","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Gastroenterology and Hepatology, Sichuan University-University of Oxford Huaxi Joint Centre for Gastrointestinal Cancer, West China Hospital, Sichuan University","place":["Chengdu, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/011ashp19","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Gastroenterology and Hepatology, Sichuan University-University of Oxford Huaxi Joint Centre for Gastrointestinal Cancer, West China Hospital, Sichuan University","place":["Chengdu, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Huimin","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/011ashp19","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Gastroenterology and Hepatology, Sichuan University-University of Oxford Huaxi Joint Centre for Gastrointestinal Cancer, West China Hospital, Sichuan University","place":["Chengdu, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Miao","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/052gg0110","id-type":"ROR","asserted-by":"publisher"}],"name":"Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine, University of Oxford","place":["Oxford, United Kingdom"]},{"id":[{"id":"https:\/\/ror.org\/05n0qbd70","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Acupuncture and Moxibustion, Fujian University of Traditional Chinese Medicine","place":["Fuzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"}]},{"given":"Ziyi","family":"Zhao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00pcrz470","id-type":"ROR","asserted-by":"publisher"}],"name":"TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine","place":["Chengdu, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"given":"David","family":"Kerr","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/052gg0110","id-type":"ROR","asserted-by":"publisher"}],"name":"Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine, University of Oxford","place":["Oxford, United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Li","family":"Yang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/011ashp19","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Gastroenterology and Hepatology, Sichuan University-University of Oxford Huaxi Joint Centre for Gastrointestinal Cancer, West China Hospital, Sichuan University","place":["Chengdu, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6267-691X","authenticated-orcid":true,"given":"Shijie","family":"Cai","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/052gg0110","id-type":"ROR","asserted-by":"publisher"}],"name":"Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine, University of Oxford","place":["Oxford, United Kingdom"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"corresponding-author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8726-7258","authenticated-orcid":true,"given":"Jilin","family":"Yang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/011ashp19","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Gastroenterology and Hepatology, Sichuan University-University of Oxford Huaxi Joint Centre for Gastrointestinal Cancer, West China Hospital, Sichuan University","place":["Chengdu, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"corresponding-author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"4374","published-online":{"date-parts":[[2024,12,19]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"1438","DOI":"10.1172\/JCI38019","type":"journal-article","article-title":"Epithelial-mesenchymal transitions: the importance of changing cell state in development and disease","volume":"119","author":"Acloque","year":"2009","journal-title":"The Journal of Clinical Investigation"},{"key":"bib2","volume-title":"AJCC Cancer Staging Manual","author":"Amin","year":"2017","type":"book"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"173","DOI":"10.21873\/anticanres.13094","type":"journal-article","article-title":"TWIST1 correlates with epithelial-mesenchymal transition markers fibronectin and VIMin adrenocortical tumors","volume":"39","author":"Bulzico","year":"2019","journal-title":"Anticancer Research"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"1297","DOI":"10.1089\/scd.2014.0471","type":"journal-article","article-title":"Novel basic helix-loop-helix transcription factor HES4 antagonizes the function of twist-1 to regulate lineage commitment of bone marrow stromal\/stem cells","volume":"24","author":"Cakouros","year":"2015","journal-title":"Stem Cells and Development"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1038\/s41580-018-0080-4","type":"journal-article","article-title":"New insights into the mechanisms of epithelial-mesenchymal transition and implications for cancer","volume":"20","author":"Dongre","year":"2019","journal-title":"Nature Reviews. Molecular Cell Biology"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"2784","DOI":"10.1002\/stem.1231","type":"journal-article","article-title":"Hes4 controls proliferative properties of neural stem cells during retinal ontogenesis","volume":"30","author":"El Yakoubi","year":"2012","journal-title":"Stem Cells"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1074\/mcp.M113.035600","type":"journal-article","article-title":"Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics","volume":"13","author":"Fagerberg","year":"2014","journal-title":"Molecular & Cellular Proteomics"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1124\/pr.111.005009","type":"journal-article","article-title":"International Union of Basic and Clinical Pharmacology. LXXXV: calcium-activated chloride channels","volume":"64","author":"Huang","year":"2012","journal-title":"Pharmacological Reviews"},{"key":"bib9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0079693","type":"journal-article","article-title":"Lineage-specific expression of bestrophin-2 and bestrophin-4 in human intestinal epithelial cells","volume":"8","author":"Ito","year":"2013","journal-title":"PLOS ONE"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"529","DOI":"10.3892\/ijo.25.2.529","type":"journal-article","article-title":"Identification and characterization of human HES2, HES3, and HES5 genes in silico","volume":"25","author":"Katoh","year":"2004","journal-title":"International Journal of Oncology"},{"key":"bib11","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-017-0192-z","type":"journal-article","article-title":"CLCA1 suppresses colorectal cancer aggressiveness via inhibition of the Wnt\/beta-catenin signaling pathway","volume":"15","author":"Li","year":"2017","journal-title":"Cell Communication and Signaling"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"1417","DOI":"10.1007\/s00424-014-1572-5","type":"journal-article","article-title":"channels","volume":"467","author":"Liu","year":"2015","journal-title":"Pflugers Archiv"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1016\/j.preteyeres.2009.04.004","type":"journal-article","article-title":"Functional roles of bestrophins in ocular epithelia","volume":"28","author":"Marmorstein","year":"2009","journal-title":"Progress in Retinal and Eye Research"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"864","DOI":"10.1161\/CIRCRESAHA.108.178517","type":"journal-article","article-title":"Bestrophin-3 (vitelliform macular dystrophy 2-like 3 protein) is essential for the cGMP-dependent calcium-activated chloride conductance in vascular smooth muscle cells","volume":"103","author":"Matchkov","year":"2008","journal-title":"Circulation Research"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"4150","DOI":"10.1158\/0008-5472.CAN-17-3009","type":"journal-article","article-title":"TWIST1 regulates vimentin through cul2 circular RNA to promote EMT in hepatocellular carcinoma","volume":"78","author":"Meng","year":"2018","journal-title":"Cancer Research"},{"key":"bib16","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-8-72","type":"journal-article","article-title":"Molecular evolution and functional divergence of the bestrophin protein family","volume":"8","author":"Milenkovic","year":"2008","journal-title":"BMC Evolutionary Biology"},{"key":"bib17","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.43231","type":"journal-article","article-title":"Molecular mechanisms of gating in the calcium-activated chloride channel bestrophin","volume":"8","author":"Miller","year":"2019","journal-title":"eLife"},{"key":"bib18","doi-asserted-by":"publisher","first-page":"5969","DOI":"10.1128\/MCB.00696-06","type":"journal-article","article-title":"Differential gene regulation by selective association of transcriptional coactivators and bZIP DNA-binding domains","volume":"26","author":"Miotto","year":"2006","journal-title":"Molecular and Cellular Biology"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"2179","DOI":"10.1002\/dvdy.21883","type":"journal-article","article-title":"Xhairy2 functions in Xenopus lens development by regulating p27(xic1) expression","volume":"238","author":"Murato","year":"2009","journal-title":"Developmental Dynamics"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"702","DOI":"10.1159\/000448860","type":"journal-article","article-title":"Impact of tight junction protein ZO-1 and TWIST expression on postoperative survival of patients with hepatocellular carcinoma","volume":"34","author":"Nagai","year":"2016","journal-title":"Digestive Diseases"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1016\/j.ydbio.2008.08.003","type":"journal-article","article-title":"Hairy2-Id3 interactions play an essential role in Xenopus neural crest progenitor specification","volume":"322","author":"Nichane","year":"2008","journal-title":"Developmental Biology"},{"key":"bib22","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1038\/s41586-019-0992-y","type":"journal-article","article-title":"Colonic epithelial cell diversity in health and inflammatory bowel disease","volume":"567","author":"Parikh","year":"2019","journal-title":"Nature"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1016\/j.tcb.2018.12.001","type":"journal-article","article-title":"EMT transition states during tumor progression and metastasis","volume":"29","author":"Pastushenko","year":"2019","journal-title":"Trends in Cell Biology"},{"key":"bib24","doi-asserted-by":"publisher","first-page":"4265","DOI":"10.1113\/jphysiol.2009.176131","type":"journal-article","article-title":"Calcium-activated chloride currents in olfactory sensory neurons from mice lacking bestrophin-2","volume":"587","author":"Pifferi","year":"2009","journal-title":"The Journal of Physiology"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"897","DOI":"10.1002\/ijc.31379","type":"journal-article","article-title":"CLCA2 epigenetic regulation by CTBP1, HDACs, ZEB1, EP300 and miR-196b-5p impacts prostate cancer cell adhesion and EMT in metabolic syndrome disease","volume":"143","author":"Porretti","year":"2018","journal-title":"International Journal of Cancer"},{"key":"bib26","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1002\/2211-5463.12554","type":"journal-article","article-title":"Wild-type p53 regulates OTOP2 transcription through DNA loop alteration of the promoter in colorectal cancer","volume":"9","author":"Qu","year":"2019","journal-title":"FEBS Open Bio"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"89","DOI":"10.5114\/pg.2018.81072","type":"journal-article","article-title":"Epidemiology of colorectal cancer: incidence, mortality, survival, and risk factors","volume":"14","author":"Rawla","year":"2019","journal-title":"Przeglad Gastroenterologiczny"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"1295","DOI":"10.1242\/jcs.061002","type":"journal-article","article-title":"Gain of oncogenic function of p53 mutants regulates E-cadherin expression uncoupled from cell invasion in colon cancer cells","volume":"123","author":"Roger","year":"2010","journal-title":"Journal of Cell Science"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1113\/expphysiol.2011.058248","type":"journal-article","article-title":"Anoctamins and gastrointestinal smooth muscle excitability","volume":"97","author":"Sanders","year":"2012","journal-title":"Experimental Physiology"},{"key":"bib30","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0115443","type":"journal-article","article-title":"Inhibition of TMEM16A expression suppresses growth and invasion in human colorectal cancer cells","volume":"9","author":"Sui","year":"2014","journal-title":"PLOS ONE"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"2802","DOI":"10.7150\/jca.19142","type":"journal-article","article-title":"HES1 promotes colorectal cancer cell resistance to 5-Fu by inducing of EMT and ABC transporter proteins","volume":"8","author":"Sun","year":"2017","journal-title":"Journal of Cancer"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"61","DOI":"10.3389\/fimmu.2022.1019582","type":"journal-article","article-title":"Comparison of MMR protein and microsatellite instability detection in colorectal cancer and its clinicopathological features analysis","volume":"50","author":"Sunlin Yong","year":"2021","journal-title":"Journal of Medical Research"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"41114","DOI":"10.1074\/jbc.M306150200","type":"journal-article","article-title":"Structure-function analysis of the bestrophin family of anion channels","volume":"278","author":"Tsunenari","year":"2003","journal-title":"The Journal of Biological Chemistry"},{"key":"bib34","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2019.109358","type":"journal-article","article-title":"Store-operated calcium entry (SOCE) contributes to phosphorylation of p38 MAPK and suppression of TNF-\u03b1 signalling in the intestinal epithelial cells","volume":"63","author":"Uwada","year":"2019","journal-title":"Cellular Signalling"},{"key":"bib35","doi-asserted-by":"publisher","first-page":"959","DOI":"10.1038\/nature03659","type":"journal-article","article-title":"Notch\/gamma-secretase inhibition turns proliferative cells in intestinal crypts and adenomas into goblet cells","volume":"435","author":"van Es","year":"2005","journal-title":"Nature"},{"key":"bib36","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1016\/j.bbrc.2007.11.151","type":"journal-article","article-title":"Twist is a transcriptional repressor of E-cadherin gene expression in breast cancer","volume":"367","author":"Vesuna","year":"2008","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"bib37","doi-asserted-by":"publisher","first-page":"2237","DOI":"10.1038\/onc.2011.392","type":"journal-article","article-title":"Loss of breast epithelial marker hCLCA2 promotes epithelial-to-mesenchymal transition and indicates higher risk of metastasis","volume":"31","author":"Walia","year":"2012","journal-title":"Oncogene"},{"key":"bib38","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-38139-5","type":"journal-article","article-title":"Notch signaling facilitates hepatitis B virus covalently closed circular DNA transcription via cAMP response element-binding protein with E3 ubiquitin ligase-modulation","volume":"9","author":"Wang","year":"2019","journal-title":"Scientific Reports"},{"key":"bib39","doi-asserted-by":"publisher","first-page":"927","DOI":"10.1016\/j.cell.2004.06.006","type":"journal-article","article-title":"Twist, a master regulator of morphogenesis, plays an essential role in tumor metastasis","volume":"117","author":"Yang","year":"2004","journal-title":"Cell"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"1570","DOI":"10.1002\/cncr.29235","type":"journal-article","article-title":"Low expression of chloride channel accessory 1 predicts a poor prognosis in colorectal cancer","volume":"121","author":"Yang","year":"2015","journal-title":"Cancer"},{"key":"bib41","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1002\/1878-0261.12017","type":"journal-article","article-title":"Epithelial-mesenchymal transition in tumor metastasis","volume":"11","author":"Yeung","year":"2017","journal-title":"Molecular Oncology"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"912","DOI":"10.1016\/j.stemcr.2020.08.001","type":"journal-article","article-title":"Androgen maintains intestinal homeostasis by inhibiting BMP signaling via intestinal dtromal cells","volume":"15","author":"Yu","year":"2020","journal-title":"Stem Cell Reports"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1007\/s10585-015-9700-y","type":"journal-article","article-title":"HES1 promotes metastasis and predicts poor survival in patients with colorectal cancer","volume":"32","author":"Yuan","year":"2015","journal-title":"Clinical & Experimental Metastasis"},{"key":"bib44","doi-asserted-by":"publisher","first-page":"110","DOI":"10.3748\/wjg.v23.i1.110","type":"journal-article","article-title":"Expression of trefoil factors and TWIST1 in colorectal cancer and their correlation with metastatic potential and prognosis","volume":"23","author":"Yusup","year":"2017","journal-title":"World Journal of Gastroenterology"},{"key":"bib45","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2019.00098","type":"journal-article","article-title":"Trends in and predictions of colorectal cancer incidence and mortality in China from 1990 to 2025","volume":"9","author":"Zhang","year":"2019","journal-title":"Frontiers in Oncology"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"e358","DOI":"10.1016\/S1470-2045(21)00343-0","type":"journal-article","article-title":"Novel therapeutic strategies: targeting epithelial-mesenchymal transition in colorectal cancer","volume":"22","author":"Zhang","year":"2021","journal-title":"The Lancet. Oncology"},{"key":"bib47","first-page":"2000","type":"journal-article","article-title":"TWIST1 is a potential prognostic marker for colorectal cancer and associated with chemoresistance","volume":"5","author":"Zhu","year":"2015","journal-title":"American Journal of Cancer Research"},{"key":"dataset1","type":"dataset","article-title":"Expression of Cystatin SN and DPT and their mechanisms in the development of colorectal cancer(CRC)","volume-title":"NCBI BioProject","author":"Wang","year":"2022","unstructured":"Wang Z. 2022. Expression of Cystatin SN and DPT and their mechanisms in the development of colorectal cancer(CRC). NCBI BioProject. http:\/\/www.ncbi.nlm.nih.gov\/bioproject\/?term=PRJNA690126. PRJNA690126."}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/88879\/elife-88879-v1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/88879\/elife-88879-v1.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/88879","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T09:56:36Z","timestamp":1787565396000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/88879"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,19]]},"references-count":48,"alternative-id":["10.7554\/eLife.88879"],"URL":"https:\/\/doi.org\/10.7554\/elife.88879","archive":["CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.07.03.547525","asserted-by":"subject"},{"id-type":"doi","id":"10.1101\/2023.07.03.547525","asserted-by":"object"}],"is-same-as":[{"id-type":"doi","id":"10.7554\/eLife.88879.3","asserted-by":"subject"}],"is-version-of":[{"id-type":"doi","id":"10.7554\/eLife.88879.1","asserted-by":"subject"},{"id-type":"doi","id":"10.7554\/eLife.88879.2","asserted-by":"subject"}],"is-supplemented-by":[{"id-type":"accession","id":"PRJNA690126","asserted-by":"subject"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,19]]},"assertion":[{"value":"single anonymised","name":"peer_review_transparency","label":"Peer review transparency","group":{"name":"peer_review_taxonomy"}},{"value":"other reviewer(s), editor","name":"peer_review_interaction","label":"Peer review interaction","group":{"name":"peer_review_taxonomy"}},{"value":"review summaries, review reports, author\/editor communication, reviewer identities reviewer opt in, editor identities","name":"peer_review_published","label":"Peer review published","group":{"name":"peer_review_taxonomy"}},{"value":"open (sign in with ORCID iD required)","name":"post_publication_commenting","label":"Post publication commenting","group":{"name":"post_publication_commenting"}}],"article-number":"RP88879"}}