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Moreover, anesthesia affects both brain functions and the stroke outcome acting as neurotoxic or protective agents. So far, no approaches were well suited to induce stroke while imaging hemodynamics along with simultaneous large-scale recording of brain functions in awake animals. For this reason, the first critical hours following the stroke insult and associated functional alteration remain poorly understood. Here, we present a strategy to investigate both stroke hemodynamics and stroke-induced functional alterations without the confounding effect of anesthesia, i.e., under awake condition. Functional ultrasound (fUS) imaging was used to continuously monitor variations in cerebral blood volume (CBV) in +65 brain regions\/hemispheres for up to 3 hr after stroke onset. The focal cortical ischemia was induced using a chemo-thrombotic agent suited for permanent middle cerebral artery occlusion in awake rats and followed by ipsi- and contralesional whiskers stimulation to investigate on the dynamic of the thalamocortical functions. Early (0\u20133 hr) and delayed (day 5) fUS recording enabled to characterize the features of the ischemia (location, CBV loss), spreading depolarizations (occurrence, amplitude) and functional alteration of the somatosensory thalamocortical circuits. Post-stroke thalamocortical functions were affected at both early and later time points (0\u20133 hr and 5 days) after stroke. Overall, our procedure facilitates early, continuous, and chronic assessments of hemodynamics and cerebral functions. When integrated with stroke studies or other pathological analyses, this approach seeks to enhance our comprehension of physiopathologies towards the development of pertinent therapeutic interventions.<\/jats:p>","DOI":"10.7554\/elife.88919","type":"journal-article","created":{"date-parts":[[2023,7,12]],"date-time":"2023-07-12T11:32:00Z","timestamp":1689161520000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.88919","source":"Crossref","is-referenced-by-count":9,"title":["Functional ultrasound imaging of stroke in awake rats"],"prefix":"10.7554","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2567-4832","authenticated-orcid":true,"given":"Cl\u00e9ment","family":"Brunner","sequence":"first","affiliation":[{"name":"Neuro-Electronics Research Flanders","place":["Leuven, Belgium"]},{"id":[{"id":"https:\/\/ror.org\/03xrhmk39","id-type":"ROR","asserted-by":"publisher"}],"name":"Vlaams Instituut voor Biotechnologie","place":["Leuven, 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