{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T11:22:30Z","timestamp":1787570550514,"version":"build-2736575974"},"reference-count":46,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T00:00:00Z","timestamp":1706140800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T00:00:00Z","timestamp":1706140800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T00:00:00Z","timestamp":1706140800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32171111"],"award-info":[{"award-number":["32171111"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["5222015"],"award-info":[{"award-number":["5222015"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Spermatogonial stem cells (SSCs) are essential for continuous spermatogenesis and male fertility. The underlying mechanisms of alternative splicing (AS) in mouse SSCs are still largely unclear. We demonstrated that SRSF1 is essential for gene expression and splicing in mouse SSCs. Crosslinking immunoprecipitation and sequencing data revealed that spermatogonia-related genes (e.g.\n                    <jats:italic>Plzf<\/jats:italic>\n                    ,\n                    <jats:italic>Id4<\/jats:italic>\n                    ,\n                    <jats:italic>Setdb1, Stra8<\/jats:italic>\n                    ,\n                    <jats:italic>Tial1<\/jats:italic>\n                    \/\n                    <jats:italic>Tiar<\/jats:italic>\n                    ,\n                    <jats:italic>Bcas2<\/jats:italic>\n                    ,\n                    <jats:italic>Ddx5<\/jats:italic>\n                    ,\n                    <jats:italic>Srsf10<\/jats:italic>\n                    ,\n                    <jats:italic>Uhrf1<\/jats:italic>\n                    , and\n                    <jats:italic>Bud31<\/jats:italic>\n                    ) were bound by SRSF1 in the mouse testes. Specific deletion of\n                    <jats:italic>Srsf1<\/jats:italic>\n                    in mouse germ cells impairs homing of precursor SSCs leading to male infertility. Whole-mount staining data showed the absence of germ cells in the testes of adult conditional knockout (cKO) mice, which indicates Sertoli cell-only syndrome in cKO mice. The expression of spermatogonia-related genes (e.g.\n                    <jats:italic>Gfra1<\/jats:italic>\n                    ,\n                    <jats:italic>Pou5f1<\/jats:italic>\n                    ,\n                    <jats:italic>Plzf<\/jats:italic>\n                    ,\n                    <jats:italic>Dnd1<\/jats:italic>\n                    ,\n                    <jats:italic>Stra8<\/jats:italic>\n                    , and\n                    <jats:italic>Taf4b<\/jats:italic>\n                    ) was significantly reduced in the testes of cKO mice. Moreover, multiomics analysis suggests that SRSF1 may affect survival of spermatogonia by directly binding and regulating\n                    <jats:italic>Tial1<\/jats:italic>\n                    \/\n                    <jats:italic>Tiar<\/jats:italic>\n                    expression through AS. In addition, immunoprecipitation mass spectrometry and co-immunoprecipitation data showed that SRSF1 interacts with RNA splicing-related proteins (e.g. SART1, RBM15, and SRSF10). Collectively, our data reveal the critical role of SRSF1 in spermatogonia survival, which may provide a framework to elucidate the molecular mechanisms of the posttranscriptional network underlying homing of precursor SSCs.\n                  <\/jats:p>","DOI":"10.7554\/elife.89316","type":"journal-article","created":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T11:31:41Z","timestamp":1691494301000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.89316","source":"Crossref","is-referenced-by-count":10,"title":["Splicing factor SRSF1 is essential for homing of precursor spermatogonial stem cells in mice"],"prefix":"10.7554","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1285-3823","authenticated-orcid":true,"given":"Longjie","family":"Sun","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04v3ywz14","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China 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