{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T19:59:23Z","timestamp":1787774363317,"version":"build-2784847793"},"reference-count":57,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2023,11,30]],"date-time":"2023-11-30T00:00:00Z","timestamp":1701302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,11,30]],"date-time":"2023-11-30T00:00:00Z","timestamp":1701302400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,11,30]],"date-time":"2023-11-30T00:00:00Z","timestamp":1701302400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000051","name":"National Human Genome Research Institute","doi-asserted-by":"crossref","award":["HG010492"],"award-info":[{"award-number":["HG010492"]}],"id":[{"id":"10.13039\/100000051","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"crossref","award":["Henikoff"],"award-info":[{"award-number":["Henikoff"]}],"id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Spermatogenesis in the\n                    <jats:italic>Drosophila<\/jats:italic>\n                    male germline proceeds through a unique transcriptional program controlled both by germline-specific transcription factors and by testis-specific versions of core transcriptional machinery. This program includes the activation of genes on the heterochromatic\n                    <jats:italic>Y<\/jats:italic>\n                    chromosome, and reduced transcription from the\n                    <jats:italic>X<\/jats:italic>\n                    chromosome, but how expression from these sex chromosomes is regulated has not been defined. To resolve this, we profiled active chromatin features in the testes from wildtype and meiotic arrest mutants and integrate this with single-cell gene expression data from the Fly Cell Atlas. These data assign the timing of promoter activation for genes with germline-enriched expression throughout spermatogenesis, and general alterations of promoter regulation in germline cells. By profiling both active RNA polymerase II and histone modifications in isolated spermatocytes, we detail widespread patterns associated with regulation of the sex chromosomes. Our results demonstrate that the\n                    <jats:italic>X<\/jats:italic>\n                    chromosome is not enriched for silencing histone modifications, implying that sex chromosome inactivation does not occur in the\n                    <jats:italic>Drosophila<\/jats:italic>\n                    male germline. Instead, a lack of dosage compensation in spermatocytes accounts for the reduced expression from this chromosome. Finally, profiling uncovers dramatic ubiquitinylation of histone H2A and lysine-16 acetylation of histone H4 across the\n                    <jats:italic>Y<\/jats:italic>\n                    chromosome in spermatocytes that may contribute to the activation of this heterochromatic chromosome.\n                  <\/jats:p>","DOI":"10.7554\/elife.89373","type":"journal-article","created":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T09:09:14Z","timestamp":1692608954000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.89373","source":"Crossref","is-referenced-by-count":20,"title":["Chromosome-specific maturation of the epigenome in the Drosophila male germline"],"prefix":"10.7554","volume":"12","author":[{"given":"James T","family":"Anderson","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/007ps6h72","id-type":"ROR","asserted-by":"publisher"}],"name":"Basic Sciences Division, Fred Hutchinson Cancer Center","place":["Seattle, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7621-8685","authenticated-orcid":true,"given":"Steven","family":"Henikoff","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/007ps6h72","id-type":"ROR","asserted-by":"publisher"}],"name":"Basic Sciences Division, Fred Hutchinson Cancer Center","place":["Seattle, United States"]},{"id":[{"id":"https:\/\/ror.org\/006w34k90","id-type":"ROR","asserted-by":"publisher"}],"name":"Howard Hughes Medical Institute","place":["Chevy Chase, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8572-6182","authenticated-orcid":true,"given":"Kami","family":"Ahmad","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/007ps6h72","id-type":"ROR","asserted-by":"publisher"}],"name":"Basic Sciences Division, Fred Hutchinson Cancer Center","place":["Seattle, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2023,11,30]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-34295-5","article-title":"Active DNA damage response signaling initiates and maintains meiotic sex chromosome inactivation","volume":"13","author":"Abe","year":"2022","journal-title":"Nature Communications"},{"key":"bib2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1009225","article-title":"The H3.3K27M oncohistone antagonizes reprogramming in Drosophila","volume":"17","author":"Ahmad","year":"2021","journal-title":"PLOS Genetics"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1016\/s1097-2765(00)80431-1","article-title":"Activation of transcription through histone H4 acetylation by MOF, an acetyltransferase essential for dosage compensation in Drosophila","volume":"5","author":"Akhtar","year":"2000","journal-title":"Molecular Cell"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"2525","DOI":"10.1242\/dev.091553","article-title":"Polycomb complexes in stem cells and embryonic development","volume":"140","author":"Aloia","year":"2013","journal-title":"Development"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"1912","DOI":"10.1073\/pnas.0910267107","article-title":"UBR2 mediates transcriptional silencing during spermatogenesis via histone ubiquitination","volume":"107","author":"An","year":"2010","journal-title":"PNAS"},{"key":"bib6","article-title":"Drosophila Transposon Canonical sequences","volume-title":"Data Set","author":"Ashburner","year":"2021","unstructured":"Ashburner M, Bayraktaroglu L, Benos T, Chillemi J, Crosby L, de Grey A, Han S, Kaminker J, Lewis S, Liao G, Reese M, Bergman C. 2021. 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