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Here, we used a murine explant model to manipulate retinal energy metabolism under entirely controlled conditions and utilised\n                    <jats:sup>1<\/jats:sup>\n                    H-NMR spectroscopy-based metabolomics, in situ enzyme detection, and cell viability readouts to uncover the pathways of retinal energy production. Our experimental manipulations resulted in varying degrees of photoreceptor degeneration, while the inner retina and retinal pigment epithelium were essentially unaffected. This selective vulnerability of photoreceptors suggested very specific adaptations in their energy metabolism. Rod photoreceptors were found to rely strongly on oxidative phosphorylation, but only mildly on glycolysis. Conversely, cone photoreceptors were dependent on glycolysis but insensitive to electron transport chain decoupling. Importantly, photoreceptors appeared to uncouple glycolytic and Krebs-cycle metabolism via three different pathways: (1) the mini-Krebs-cycle, fuelled by glutamine and branched chain amino acids, generating\n                    <jats:italic>N<\/jats:italic>\n                    -acetylaspartate; (2) the alanine-generating Cahill-cycle; (3) the lactate-releasing Cori-cycle. Moreover, the metabolomics data indicated a shuttling of taurine and hypotaurine between the retinal pigment epithelium and photoreceptors, likely resulting in an additional net transfer of reducing power to photoreceptors. These findings expand our understanding of retinal physiology and pathology and shed new light on neuronal energy homeostasis and the pathogenesis of neurodegenerative diseases.\n                  <\/jats:p>","DOI":"10.7554\/elife.91141","type":"journal-article","created":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T09:39:05Z","timestamp":1695893945000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.91141","source":"Crossref","is-referenced-by-count":28,"title":["Retinal metabolism displays evidence for uncoupling of glycolysis and oxidative phosphorylation via Cori-, Cahill-, and mini-Krebs-cycle"],"prefix":"10.7554","volume":"12","author":[{"given":"Yiyi","family":"Chen","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03a1kwz48","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Ophthalmic Research, University of T\u00fcbingen","place":["Tuebingen, 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