{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:40:43Z","timestamp":1787568043050,"version":"build-2736575974"},"reference-count":64,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T00:00:00Z","timestamp":1728864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T00:00:00Z","timestamp":1728864000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T00:00:00Z","timestamp":1728864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["1R01DE031271-01-A11"],"award-info":[{"award-number":["1R01DE031271-01-A11"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["DE026762"],"award-info":[{"award-number":["DE026762"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01AR062920"],"award-info":[{"award-number":["R01AR062920"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01AR062368"],"award-info":[{"award-number":["R01AR062368"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Current treatments for congenital and acquired craniofacial (CF) bone abnormalities are limited and costly. Conventional methods involve surgical correction, short-term stabilization, and long-term bone grafting, which may include problematic allografts and limited autografts. While bone morphogenetic protein 2 (BMP2) has been used for bone regeneration, it can cause bone overgrowth and life-threatening inflammation. Bone marrow-derived mesenchymal stem cell therapies, though promising, are not Food and Drug Administration approved and are resource intensive. Thus, there is a need for effective, affordable, and less side-effect-prone bone regenerative therapies. Previous research demonstrated that JAGGED1 induces osteoblast commitment in murine cranial neural crest cells through a NOTCH-dependent non-canonical pathway involving JAK2\u2013STAT5. We hypothesize that delivery of JAGGED1 and induction of its downstream NOTCH non-canonical signaling in pediatric human osteoblasts constitutes an effective bone regenerative treatment. Delivering pediatric human bone-derived osteoblast-like cells to an in vivo murine bone loss model of a critically sized cranial defect, we identified that JAGGED1 promotes human pediatric osteoblast commitment and bone formation through p70 S6K phosphorylation. This approach highlights the potential of JAGGED1 and its downstream activators as innovative treatments for pediatric CF bone loss.<\/jats:p>","DOI":"10.7554\/elife.92925","type":"journal-article","created":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T19:34:36Z","timestamp":1704483276000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.92925","source":"Crossref","is-referenced-by-count":3,"title":["Delivery of a Jagged1-PEG-MAL hydrogel with pediatric human bone cells regenerates critically sized craniofacial bone defects"],"prefix":"10.7554","volume":"13","author":[{"given":"Archana","family":"Kamalakar","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03czfpz43","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pediatric Otolaryngology, Emory University","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Brendan","family":"Tobin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zkghx44","id-type":"ROR","asserted-by":"publisher"}],"name":"Parker H. Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology","place":["Atlanta, United States"]},{"name":"School of Chemistry and Biomolecular Engineering, Georgia Tech College of Engineering","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"project-administration","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Sundus","family":"Kaimari","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03czfpz43","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pediatric Otolaryngology, Emory University","place":["Atlanta, United States"]},{"id":[{"id":"https:\/\/ror.org\/01zkghx44","id-type":"ROR","asserted-by":"publisher"}],"name":"Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4255-1308","authenticated-orcid":true,"given":"M Hope","family":"Robinson","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03czfpz43","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pediatric Otolaryngology, Emory University","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Afra I","family":"Toma","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03czfpz43","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pediatric Otolaryngology, Emory University","place":["Atlanta, United States"]},{"id":[{"id":"https:\/\/ror.org\/01zkghx44","id-type":"ROR","asserted-by":"publisher"}],"name":"Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Timothy","family":"Cha","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03czfpz43","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pediatric Otolaryngology, Emory University","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Samir","family":"Chihab","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03czfpz43","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Orthopedics, Emory University","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"}]},{"given":"Irica","family":"Moriarity","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zkghx44","id-type":"ROR","asserted-by":"publisher"}],"name":"Neuroscience Program in College of Sciences, Georgia Institute of Technology","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"}]},{"given":"Surabhi","family":"Gautam","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03czfpz43","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Orthopedics, Emory University","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"}]},{"given":"Pallavi","family":"Bhattaram","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03czfpz43","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Orthopedics, Emory University","place":["Atlanta, United States"]},{"name":"The Atlanta Veterans Affairs Medical Center Atlanta","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"}]},{"given":"Shelly","family":"Abramowicz","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03czfpz43","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pediatric Otolaryngology, Emory University","place":["Atlanta, United States"]},{"id":[{"id":"https:\/\/ror.org\/03czfpz43","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Surgery, Division of Oral and Maxillofacial Surgery, Emory University","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"resources","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3322-281X","authenticated-orcid":true,"given":"Hicham","family":"Drissi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03czfpz43","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Orthopedics, Emory University","place":["Atlanta, United States"]},{"name":"The Atlanta Veterans Affairs Medical Center Atlanta","place":["Atlanta, United States"]},{"id":[{"id":"https:\/\/ror.org\/03czfpz43","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Cell Biology, Emory University","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"project-administration","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"given":"Andres","family":"Garcia","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zkghx44","id-type":"ROR","asserted-by":"publisher"}],"name":"Parker H. Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology","place":["Atlanta, United States"]},{"name":"George W. Woodruff School of Mechanical Engineering, Georgia Tech College of Engineering","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"project-administration","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"given":"Levi","family":"Wood","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zkghx44","id-type":"ROR","asserted-by":"publisher"}],"name":"Parker H. Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology","place":["Atlanta, United States"]},{"id":[{"id":"https:\/\/ror.org\/01zkghx44","id-type":"ROR","asserted-by":"publisher"}],"name":"Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology","place":["Atlanta, United States"]},{"name":"George W. Woodruff School of Mechanical Engineering, Georgia Tech College of Engineering","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"project-administration","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8255-3965","authenticated-orcid":true,"given":"Steven L","family":"Goudy","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/050fhx250","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pediatric Otolaryngology, Children\u2019s Healthcare of Atlanta","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"corresponding-author","vocabulary":"crossref"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]}],"member":"4374","published-online":{"date-parts":[[2024,10,14]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4933-10-23","type":"journal-article","article-title":"Platelet-rich plasma (PRP) in dental and oral surgery: from the wound healing to bone regeneration","volume":"10","author":"Albanese","year":"2013","journal-title":"Immunity & Ageing"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1177\/1071100713511434","type":"journal-article","article-title":"Site selection and pain outcome after autologous bone graft harvest","volume":"35","author":"Baumhauer","year":"2014","journal-title":"Foot & Ankle International"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"1468","DOI":"10.1002\/jbmr.141","type":"journal-article","article-title":"Guidelines for assessment of bone microstructure in rodents using micro-computed tomography","volume":"25","author":"Bouxsein","year":"2010","journal-title":"Journal of Bone and Mineral Research"},{"key":"bib4","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02182","type":"journal-article","article-title":"Chemokines in physiological and pathological bone remodeling","volume":"10","author":"Brylka","year":"2019","journal-title":"Frontiers in Immunology"},{"key":"bib5","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/318714","type":"journal-article","article-title":"ROS, Notch, and Wnt signaling pathways: crosstalk between three major regulators of cardiovascular biology","volume":"2014","author":"Caliceti","year":"2014","journal-title":"BioMed Research International"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"1759","DOI":"10.1902\/jop.2010.100235","type":"journal-article","article-title":"Analysis of complications following augmentation with cancellous block allografts","volume":"81","author":"Chaushu","year":"2010","journal-title":"Journal of Periodontology"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1055\/s-0030-1255336","type":"journal-article","article-title":"Reconstruction of mandibular defects","volume":"24","author":"Chim","year":"2010","journal-title":"Seminars in Plastic Surgery"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"e124","DOI":"10.1097\/TA.0000000000002589","type":"journal-article","article-title":"Review of facial trauma management","volume":"88","author":"Choi","year":"2020","journal-title":"The Journal of Trauma and Acute Care Surgery"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1111\/j.1708-8208.2009.00221.x","type":"journal-article","article-title":"Iliac crest autogenous bone graft versus alloplastic graft and guided bone regeneration in the reconstruction of atrophic maxillae: a 5-year retrospective study on cost-effectiveness and clinical outcome","volume":"13","author":"Dahlin","year":"2011","journal-title":"Clinical Implant Dentistry and Related Research"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"200","DOI":"10.4103\/0970-0358.73452","type":"journal-article","article-title":"Psychological issues in acquired facial trauma","volume":"43","author":"De Sousa","year":"2010","journal-title":"Indian Journal of Plastic Surgery"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"1558","DOI":"10.1002\/jbm.a.34825","type":"journal-article","article-title":"Jagged1 immobilization to an osteoconductive polymer activates the Notch signaling pathway and induces osteogenesis","volume":"102","author":"Dishowitz","year":"2014","journal-title":"Journal of Biomedical Materials Research. Part A"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1055\/s-0029-1215875","type":"journal-article","article-title":"Bone grafts in craniofacial surgery","volume":"2","author":"Elsalanty","year":"2009","journal-title":"Craniomaxillofacial Trauma & Reconstruction"},{"key":"bib13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0042967","type":"journal-article","article-title":"Low bone turnover and low BMD in Down syndrome: effect of intermittent PTH treatment","volume":"7","author":"Fowler","year":"2012","journal-title":"PLOS ONE"},{"key":"bib14","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/6025918","type":"journal-article","article-title":"A multicentric, open-label, randomized, comparative clinical trial of two different doses of expanded hBM-MSCs plus biomaterial versus iliac crest autograft, for bone healing in nonunions after long bone fractures: Study Protocol","volume":"2018","author":"G\u00f3mez-Barrena","year":"2018","journal-title":"Stem Cells International"},{"key":"bib15","doi-asserted-by":"publisher","DOI":"10.4103\/0970-9290.87084","type":"journal-article","article-title":"Bone allografts: a review of safety and efficacy","volume":"22","author":"Grover","year":"2011","journal-title":"Indian Journal of Dental Research"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"2632","DOI":"10.1074\/jbc.M111.323709","type":"journal-article","article-title":"p38\u03b1 mediates cell survival in response to oxidative stress via induction of antioxidant genes: effect on the p70S6K pathway","volume":"287","author":"Guti\u00e9rrez-Uzquiza","year":"2012","journal-title":"The Journal of Biological Chemistry"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"1186","DOI":"10.1016\/j.joca.2014.06.017","type":"journal-article","article-title":"Exaggerated inflammatory environment decreases BMP-2\/ACS-induced ectopic bone mass in a rat model: implications for clinical use of BMP-2","volume":"22","author":"Huang","year":"2014","journal-title":"Osteoarthritis and Cartilage"},{"key":"bib18","doi-asserted-by":"publisher","first-page":"23065","DOI":"10.1074\/jbc.M200328200","type":"journal-article","article-title":"C-Raf\/MEK\/ERK pathway controls protein kinase C-mediated p70S6K activation in adult cardiac muscle cells","volume":"277","author":"Iijima","year":"2002","journal-title":"The Journal of Biological Chemistry"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1096\/fba.2021-00092","type":"journal-article","article-title":"New insights into the role of matrix metalloproteinase 3 (MMP3) in bone","volume":"4","author":"Jehan","year":"2022","journal-title":"FASEB bioAdvances"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"1811","DOI":"10.3892\/ol.2017.5647","type":"journal-article","article-title":"Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze-dried bone marrow stem cell paracrine factors","volume":"13","author":"Kakabadze","year":"2017","journal-title":"Oncology Letters"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1016\/j.cellsig.2018.12.002","type":"journal-article","article-title":"A non-canonical JAGGED1 signal to JAK2 mediates osteoblast commitment in cranial neural crest cells","volume":"54","author":"Kamalakar","year":"2019","journal-title":"Cellular Signalling"},{"key":"bib22","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2020.115657","type":"journal-article","article-title":"JAGGED1 stimulates cranial neural crest cell osteoblast commitment pathways and bone regeneration independent of canonical NOTCH signaling","volume":"143","author":"Kamalakar","year":"2021","journal-title":"Bone"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1007\/978-1-4419-1050-9_5","type":"journal-article","article-title":"Regulation of osteoblast differentiation by Runx2","volume":"658","author":"Komori","year":"2010","journal-title":"Advances in Experimental Medicine and Biology"},{"key":"bib24","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-11596-9","type":"journal-article","article-title":"Non-canonical Wnt signaling participates in Jagged1-induced osteo\/odontogenic differentiation in human dental pulp stem cells","volume":"12","author":"Kornsuthisopon","year":"2022","journal-title":"Scientific Reports"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1007\/s12663-015-0766-5","type":"journal-article","article-title":"Mandibular reconstruction: overview","volume":"15","author":"Kumar","year":"2016","journal-title":"Journal of Maxillofacial and Oral Surgery"},{"key":"bib26","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.bone.2018.07.013","type":"journal-article","article-title":"Real-world effectiveness of teriparatide on fracture reduction in patients with osteoporosis and comorbidities or risk factors for fractures: Integrated analysis of 4 prospective observational studies","volume":"116","author":"Langdahl","year":"2018","journal-title":"Bone"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"933","DOI":"10.1097\/00006534-198406000-00014","type":"journal-article","article-title":"Donor-site morbidity after harvesting rib and iliac bone","volume":"73","author":"Laurie","year":"1984","journal-title":"Plastic and Reconstructive Surgery"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"5301","DOI":"10.4049\/jimmunol.179.8.5301","type":"journal-article","article-title":"STAT5 is essential for Akt\/p70S6 kinase activity during IL-2-induced lymphocyte proliferation","volume":"179","author":"Lockyer","year":"2007","journal-title":"Journal of Immunolog"},{"key":"bib29","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8","type":"journal-article","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biology"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"1495","DOI":"10.1016\/j.ajpath.2019.05.005","type":"journal-article","article-title":"Notch signaling in osteogenesis, osteoclastogenesis, and angiogenesis","volume":"189","author":"Luo","year":"2019","journal-title":"The American Journal of Pathology"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"703","DOI":"10.1038\/s41596-019-0128-8","type":"journal-article","article-title":"Protocol update for large-scale genome and gene function analysis with the PANTHER classification system (v.14.0)","volume":"14","author":"Mi","year":"2019","journal-title":"Nature Protocols"},{"key":"bib32","first-page":"3643","type":"journal-article","article-title":"Caffeine induces apoptosis of osteosarcoma cells by inhibiting AKT\/mTOR\/S6K, NF-\u03baB and MAPK pathways","volume":"32","author":"Miwa","year":"2012","journal-title":"Anticancer Research"},{"key":"bib33","doi-asserted-by":"publisher","DOI":"10.1186\/s13244-020-0837-0","type":"journal-article","article-title":"Imaging of mandibular fractures: a pictorial review","volume":"11","author":"Nardi","year":"2020","journal-title":"Insights into Imaging"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"15842","DOI":"10.1074\/jbc.M412891200","type":"journal-article","article-title":"Critical regulation of bone morphogenetic protein-induced osteoblastic differentiation by Delta1\/Jagged1-activated Notch1 signaling","volume":"280","author":"Nobta","year":"2005","journal-title":"The Journal of Biological Chemistry"},{"key":"bib35","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21217967","type":"journal-article","article-title":"Distinct osteogenic potentials of BMP-2 and FGF-2 in extramedullary and medullary microenvironments","volume":"21","author":"Nosho","year":"2020","journal-title":"International Journal of Molecular Sciences"},{"key":"bib36","doi-asserted-by":"publisher","first-page":"2244","DOI":"10.1210\/en.2008-1597","type":"journal-article","article-title":"The chemokine Cxcl1 is a novel target gene of parathyroid hormone (PTH)\/PTH-related protein in committed osteoblasts","volume":"150","author":"Onan","year":"2009","journal-title":"Endocrinology"},{"key":"bib37","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1016\/j.archoralbio.2019.01.013","type":"journal-article","article-title":"Jagged1 promotes mineralization in human bone-derived cells","volume":"99","author":"Osathanon","year":"2019","journal-title":"Archives of Oral Biology"},{"key":"bib38","doi-asserted-by":"publisher","first-page":"6065","DOI":"10.1158\/0008-5472.CAN-08-2409","type":"journal-article","article-title":"The wnt target jagged-1 mediates the activation of notch signaling by progastrin in human colorectal cancer cells","volume":"69","author":"Pannequin","year":"2009","journal-title":"Cancer Research"},{"key":"bib39","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1002\/adma.201103574","type":"journal-article","article-title":"Maleimide cross-linked bioactive PEG hydrogel exhibits improved reaction kinetics and cross-linking for cell encapsulation and in situ delivery","volume":"24","author":"Phelps","year":"2012","journal-title":"Advanced Materials"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"2353","DOI":"10.1158\/0008-5472.CAN-04-1890","type":"journal-article","article-title":"Expression of Notch-1 and its ligands, Delta-like-1 and Jagged-1, is critical for glioma cell survival and proliferation","volume":"65","author":"Purow","year":"2005","journal-title":"Cancer Research"},{"key":"bib41","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1007\/s11914-013-0145-4","type":"journal-article","article-title":"Notch signaling and bone remodeling","volume":"11","author":"Regan","year":"2013","journal-title":"Current Osteoporosis Reports"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"114","DOI":"10.4161\/org.23306","type":"journal-article","article-title":"Bone grafts, bone substitutes and orthobiologics: the bridge between basic science and clinical advancements in fracture healing","volume":"8","author":"Roberts","year":"2012","journal-title":"Organogenesis"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1016\/j.joms.2009.09.045","type":"journal-article","article-title":"Effect of intermittent systemic administration of recombinant parathyroid hormone (1-34) on mandibular fracture healing in rats","volume":"68","author":"Rowshan","year":"2010","journal-title":"Journal of Oral and Maxillofacial Surgery"},{"key":"bib44","doi-asserted-by":"publisher","first-page":"2199","DOI":"10.1101\/gad.351605","type":"journal-article","article-title":"Ribosomal protein S6 phosphorylation is a determinant of cell size and glucose homeostasis","volume":"19","author":"Ruvinsky","year":"2005","journal-title":"Genes & Development"},{"key":"bib45","doi-asserted-by":"publisher","first-page":"342","DOI":"10.1016\/j.tibs.2006.04.003","type":"journal-article","article-title":"Ribosomal protein S6 phosphorylation: from protein synthesis to cell size","volume":"31","author":"Ruvinsky","year":"2006","journal-title":"Trends in Biochemical Sciences"},{"key":"bib46","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005618","type":"journal-article","article-title":"Mice deficient in ribosomal protein S6 phosphorylation suffer from muscle weakness that reflects a growth defect and energy deficit","volume":"4","author":"Ruvinsky","year":"2009","journal-title":"PLOS ONE"},{"key":"bib47","doi-asserted-by":"publisher","first-page":"D10","DOI":"10.1093\/nar\/gkaa892","type":"journal-article","article-title":"Database resources of the national center for biotechnology information","volume":"49","author":"Sayers","year":"2021","journal-title":"Nucleic Acids Research"},{"key":"bib48","doi-asserted-by":"publisher","first-page":"902","DOI":"10.1097\/01.prs.0000200069.81973.49","type":"journal-article","article-title":"Mandibular reconstruction using a combination graft of rhBMP-2 with bone marrow cells expanded in vitro","volume":"117","author":"Seto","year":"2006","journal-title":"Plastic and Reconstructive Surgery"},{"key":"bib49","doi-asserted-by":"publisher","first-page":"54e","DOI":"10.1097\/PRS.0000000000007451","type":"journal-article","article-title":"Craniofacial and long bone development in the context of distraction osteogenesis","volume":"147","author":"Shah","year":"2021","journal-title":"Plastic and Reconstructive Surgery"},{"key":"bib50","doi-asserted-by":"publisher","first-page":"528","DOI":"10.1016\/s0168-8278(03)00291-5","type":"journal-article","article-title":"Regulation of ERK\/JNK\/p70S6K in two rat models of liver injury and fibrosis","volume":"39","author":"Svegliati-Baroni","year":"2003","journal-title":"Journal of Hepatology"},{"key":"bib51","doi-asserted-by":"publisher","first-page":"1049","DOI":"10.1177\/0885328216686727","type":"journal-article","article-title":"The potential of stromal cell-derived factor-1 delivery using a collagen membrane for bone regeneration","volume":"31","author":"Takayama","year":"2017","journal-title":"Journal of Biomaterials Applications"},{"key":"bib52","doi-asserted-by":"publisher","first-page":"552","DOI":"10.1016\/j.spinee.2013.08.060","type":"journal-article","article-title":"Complications with the use of bone morphogenetic protein 2 (BMP-2) in spine surgery","volume":"14","author":"Tannoury","year":"2014","journal-title":"The Spine Journal"},{"key":"bib53","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1002\/pro.4218","type":"journal-article","article-title":"PANTHER: Making genome-scale phylogenetics accessible to all","volume":"31","author":"Thomas","year":"2022","journal-title":"Protein Science"},{"key":"bib54","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.10693","type":"journal-article","article-title":"Trends in the incidence, prevalence and years lived with disability of facial fracture at global, regional and national levels from 1990 to 2017","volume":"9","author":"Wu","year":"2021","journal-title":"PeerJ"},{"key":"bib55","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1016\/j.exphem.2008.09.001","type":"journal-article","article-title":"Role of NF-\u03baB in hematopoietic niche function of osteoblasts after radiation injury","volume":"37","author":"Xiao","year":"2009","journal-title":"Experimental Hematology"},{"key":"bib56","doi-asserted-by":"publisher","first-page":"849","DOI":"10.1016\/j.bmcl.2010.11.069","type":"journal-article","article-title":"Potent and selective thiophene urea-templated inhibitors of S6K","volume":"21","author":"Ye","year":"2011","journal-title":"Bioorganic & Medicinal Chemistry Letters"},{"key":"bib57","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1016\/j.bone.2016.07.006","type":"journal-article","article-title":"Jagged1 expression by osteoblast-lineage cells regulates trabecular bone mass and periosteal expansion in mice","volume":"91","author":"Youngstrom","year":"2016","journal-title":"Bone"},{"key":"bib58","doi-asserted-by":"publisher","DOI":"10.1038\/s41536-017-0037-9","type":"journal-article","article-title":"Intraoperative delivery of the Notch ligand Jagged-1 regenerates appendicular and craniofacial bone defects","volume":"2","author":"Youngstrom","year":"2017","journal-title":"NPJ Regenerative Medicine"},{"key":"bib59","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1007\/s00223-011-9541-x","type":"journal-article","article-title":"Notch regulation of bone development and remodeling and related skeletal disorders","volume":"90","author":"Zanotti","year":"2012","journal-title":"Calcified Tissue International"},{"key":"bib60","doi-asserted-by":"publisher","first-page":"3200","DOI":"10.1172\/JCI68901","type":"journal-article","article-title":"NOTCH inhibits osteoblast formation in inflammatory arthritis via noncanonical NF-\u03baB","volume":"124","author":"Zhang","year":"2014","journal-title":"The Journal of Clinical Investigation"},{"key":"bib61","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-15-S10-P16","type":"journal-article","article-title":"Heatmap3: an improved heatmap package with more powerful and convenient features","volume":"15","author":"Zhao","year":"2014","journal-title":"BMC Bioinformatics"},{"key":"bib62","doi-asserted-by":"publisher","first-page":"478","DOI":"10.1203\/PDR.0b013e31816bdf36","type":"journal-article","article-title":"Tissue engineering craniofacial defects with adult stem cells? Are we ready yet?","volume":"63","author":"Zuk","year":"2008","journal-title":"Pediatric Research"},{"key":"bib63","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1016\/j.taap.2019.04.024","type":"journal-article","article-title":"Alpha ketoglutarate exerts a pro-osteogenic effect in osteoblast cell lines through activation of JNK and mTOR\/S6K1\/S6 signaling pathways","volume":"374","author":"\u017burek","year":"2019","journal-title":"Toxicology and Applied Pharmacology"},{"key":"dataset1","type":"dataset","article-title":"Delivery of a Jagged1-PEG-MAL hydrogel with pediatric human bone cells regenerates critically sized craniofacial bone defects","volume-title":"NCBI Gene Expression Omnibus","author":"Kamalakar","year":"2024","unstructured":"Kamalakar A, Tobin B, Kaimari S, Robinson MH, Toma AI, Cha T, Chihab S, Moriarity I, Gautam S, Bhattaram P, Abramowicz S, Drissi H, Garc\u00eda AJ, Wood LB, Goudy SL. 2024. Delivery of a Jagged1-PEG-MAL hydrogel with pediatric human bone cells regenerates critically sized craniofacial bone defects. NCBI Gene Expression Omnibus. https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE274096. GSE274096."}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/92925\/elife-92925-v1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/92925\/elife-92925-v1.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/92925","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:09:38Z","timestamp":1787566178000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/92925"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,14]]},"references-count":64,"alternative-id":["10.7554\/eLife.92925"],"URL":"https:\/\/doi.org\/10.7554\/elife.92925","archive":["CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.10.06.561291","asserted-by":"subject"}],"is-same-as":[{"id-type":"doi","id":"10.7554\/eLife.92925.3","asserted-by":"subject"}],"is-version-of":[{"id-type":"doi","id":"10.7554\/eLife.92925.1","asserted-by":"subject"},{"id-type":"doi","id":"10.7554\/eLife.92925.2","asserted-by":"subject"}],"is-supplemented-by":[{"id-type":"accession","id":"GSE274096","asserted-by":"subject"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,14]]},"assertion":[{"value":"single anonymised","name":"peer_review_transparency","label":"Peer review transparency","group":{"name":"peer_review_taxonomy"}},{"value":"other reviewer(s), editor","name":"peer_review_interaction","label":"Peer review interaction","group":{"name":"peer_review_taxonomy"}},{"value":"review summaries, review reports, author\/editor communication, reviewer identities reviewer opt in, editor identities","name":"peer_review_published","label":"Peer review published","group":{"name":"peer_review_taxonomy"}},{"value":"open (sign in with ORCID iD required)","name":"post_publication_commenting","label":"Post publication commenting","group":{"name":"post_publication_commenting"}}],"article-number":"RP92925"}}