{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:40:44Z","timestamp":1787568044574,"version":"build-2736575974"},"reference-count":51,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2024,11,13]],"date-time":"2024-11-13T00:00:00Z","timestamp":1731456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,11,13]],"date-time":"2024-11-13T00:00:00Z","timestamp":1731456000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,11,13]],"date-time":"2024-11-13T00:00:00Z","timestamp":1731456000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014989","name":"Chan Zuckerberg Initiative","doi-asserted-by":"publisher","award":["2021-240504"],"award-info":[{"award-number":["2021-240504"]}],"id":[{"id":"10.13039\/100014989","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014989","name":"Chan Zuckerberg Initiative","doi-asserted-by":"publisher","award":["GBI-0000000093"],"award-info":[{"award-number":["GBI-0000000093"]}],"id":[{"id":"10.13039\/100014989","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021778","name":"Agencia Nacional de Promoci\u00f3n de la Investigaci\u00f3n, el Desarrollo Tecnol\u00f3gico y la Innovaci\u00f3n","doi-asserted-by":"publisher","award":["2020-00988"],"award-info":[{"award-number":["2020-00988"]}],"id":[{"id":"10.13039\/501100021778","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["R01HD106968"],"award-info":[{"award-number":["R01HD106968"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021778","name":"Agencia Nacional de Promoci\u00f3n de la Investigaci\u00f3n, el Desarrollo Tecnol\u00f3gico y la Innovaci\u00f3n","doi-asserted-by":"publisher","award":["2017-3047"],"award-info":[{"award-number":["2017-3047"]}],"id":[{"id":"10.13039\/501100021778","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014989","name":"Chan Zuckerberg Initiative","doi-asserted-by":"publisher","award":["2022-252509"],"award-info":[{"award-number":["2022-252509"]}],"id":[{"id":"10.13039\/100014989","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT"],"award-info":[{"award-number":["PICT"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["2018-1988"],"award-info":[{"award-number":["2018-1988"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006087","name":"Direcci\u00f3n General de Asuntos del Personal Acad\u00e9mico, Universidad Nacional Aut\u00f3noma de M\u00e9xico","doi-asserted-by":"publisher","award":["IN105222"],"award-info":[{"award-number":["IN105222"]}],"id":[{"id":"10.13039\/501100006087","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006087","name":"Direcci\u00f3n General de Asuntos del Personal Acad\u00e9mico, Universidad Nacional Aut\u00f3noma de M\u00e9xico","doi-asserted-by":"publisher","award":["IN211821"],"award-info":[{"award-number":["IN211821"]}],"id":[{"id":"10.13039\/501100006087","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006087","name":"Direcci\u00f3n General de Asuntos del Personal Acad\u00e9mico, Universidad Nacional Aut\u00f3noma de M\u00e9xico","doi-asserted-by":"publisher","award":["IN200919"],"award-info":[{"award-number":["IN200919"]}],"id":[{"id":"10.13039\/501100006087","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HD380882"],"award-info":[{"award-number":["R01HD380882"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Mammalian sperm delve into the female reproductive tract to fertilize the female gamete. The available information about how sperm regulate their motility during the final journey to the fertilization site is extremely limited. In this work, we investigated the structural and functional changes in the sperm flagellum after acrosomal exocytosis (AE) and during the interaction with the eggs. The evidence demonstrates that the double helix actin network surrounding the mitochondrial sheath of the midpiece undergoes structural changes prior to the motility cessation. This structural modification is accompanied by a decrease in diameter of the midpiece and is driven by intracellular calcium changes that occur concomitant with a reorganization of the actin helicoidal cortex. Midpiece contraction occurs in a subset of cells that undergo AE, and live-cell imaging during in vitro fertilization showed that the midpiece contraction is required for motility cessation after fusion is initiated. These findings provide the first evidence of the F-actin network\u2019s role in regulating sperm motility, adapting its function to meet specific cellular requirements during fertilization, and highlighting the broader significance of understanding sperm motility.<\/jats:p>","DOI":"10.7554\/elife.93792","type":"journal-article","created":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T10:25:08Z","timestamp":1705573508000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.93792","source":"Crossref","is-referenced-by-count":0,"title":["Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization"],"prefix":"10.7554","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6465-0609","authenticated-orcid":true,"given":"Martina","family":"Jablo\u00f1ski","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03cqe8w59","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Biologi\u0301a y Medicina Experimental, Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas (IBYME-CONICET)","place":["Buenos Aires, Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"data-curation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2745-8236","authenticated-orcid":true,"given":"Guillermina M","family":"Luque","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03cqe8w59","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Biologi\u0301a y Medicina Experimental, Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas (IBYME-CONICET)","place":["Buenos Aires, Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Matias","family":"Gomez Elias","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03cqe8w59","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Biologi\u0301a y Medicina Experimental, Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas (IBYME-CONICET)","place":["Buenos Aires, Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Claudia","family":"Sanchez Cardenas","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01tmp8f25","id-type":"ROR","asserted-by":"publisher"}],"name":"Departamento de Gen\u00e9tica del Desarrollo y Fisiolog\u00eda Molecular, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico","place":["Morelos, Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Xinran","family":"Xu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03k1gpj17","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Electrical and Computer Engineering and School of Biomedical Engineering, Colorado State University","place":["Fort Collins, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Jose L","family":"de La Vega Beltran","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01tmp8f25","id-type":"ROR","asserted-by":"publisher"}],"name":"Departamento de Gen\u00e9tica del Desarrollo y Fisiolog\u00eda Molecular, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico","place":["Morelos, Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Gabriel","family":"Corkidi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01tmp8f25","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratorio de Im\u00e1genes y Visi\u00f3n por Computadora, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico","place":["Morelos, Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Alejandro","family":"Linares","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01tmp8f25","id-type":"ROR","asserted-by":"publisher"}],"name":"Departamento de Gen\u00e9tica del Desarrollo y Fisiolog\u00eda Molecular, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico","place":["Morelos, Mexico"]},{"id":[{"id":"https:\/\/ror.org\/01tmp8f25","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratorio Nacional de Microscop\u00eda Avanzada, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico","place":["Morelos, Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Victor","family":"Abonza","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01tmp8f25","id-type":"ROR","asserted-by":"publisher"}],"name":"Departamento de Gen\u00e9tica del Desarrollo y Fisiolog\u00eda Molecular, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico","place":["Morelos, Mexico"]},{"id":[{"id":"https:\/\/ror.org\/01tmp8f25","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratorio Nacional de Microscop\u00eda Avanzada, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico","place":["Morelos, Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Aquetzalli","family":"Arenas-Hernandez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04z3afh10","id-type":"ROR","asserted-by":"publisher"}],"name":"Departamento de Microscop\u00eda Electr\u00f3nica, Instituto Nacional de Cancerolog\u00eda","place":["Mexico, Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Mar\u00eda DP","family":"Ramos-Godinez","sequence":"additional","affiliation":[{"name":"Unidad de Aplicaciones Avanzadas en Microscop\u00eda, Instituto Nacional de Cancerolog\u00eda Unidad de Investigaci\u00f3n Biom\u00e9dica en C\u00e1ncer","place":["Mexico, Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Alejandro","family":"L\u00f3pez-Saavedra","sequence":"additional","affiliation":[{"name":"Unidad de Aplicaciones Avanzadas en Microscop\u00eda, Instituto Nacional de Cancerolog\u00eda Unidad de Investigaci\u00f3n Biom\u00e9dica en C\u00e1ncer","place":["Mexico, Mexico"]},{"id":[{"id":"https:\/\/ror.org\/03ayjn504","id-type":"ROR","asserted-by":"publisher"}],"name":"Tecnologico de Monterrey, Escuela de Medicina y Ciencias de la Salud","place":["Mexico City, Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"resources","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7607-1954","authenticated-orcid":true,"given":"Dario","family":"Krapf","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04x0n3178","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Biologi\u0301a Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas (CONICET), Universidad Nacional de Rosario (UNR)","place":["Rosario, Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"}]},{"given":"Diego","family":"Krapf","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03k1gpj17","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Electrical and Computer Engineering and School of Biomedical Engineering, Colorado State University","place":["Fort Collins, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Alberto","family":"Darszon","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01tmp8f25","id-type":"ROR","asserted-by":"publisher"}],"name":"Departamento de Gen\u00e9tica del Desarrollo y Fisiolog\u00eda Molecular, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico","place":["Morelos, Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"}]},{"given":"Ad\u00e1n","family":"Guerrero","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01tmp8f25","id-type":"ROR","asserted-by":"publisher"}],"name":"Departamento de Gen\u00e9tica del Desarrollo y Fisiolog\u00eda Molecular, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico","place":["Morelos, Mexico"]},{"id":[{"id":"https:\/\/ror.org\/01tmp8f25","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratorio Nacional de Microscop\u00eda Avanzada, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico","place":["Morelos, Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"corresponding-author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7163-6482","authenticated-orcid":true,"given":"Mariano G","family":"Buffone","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03cqe8w59","id-type":"ROR","asserted-by":"publisher"}],"name":"Instituto de Biologi\u0301a y Medicina Experimental, Consejo Nacional de Investigaciones Cienti\u0301ficas y Te\u0301cnicas (IBYME-CONICET)","place":["Buenos Aires, Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"corresponding-author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"4374","published-online":{"date-parts":[[2024,11,13]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1002\/mrd.21393","type":"journal-article","article-title":"Heads or tails? Structural events and molecular mechanisms that promote mammalian sperm acrosomal exocytosis and motility","volume":"79","author":"Buffone","year":"2012","journal-title":"Molecular Reproduction and Development"},{"key":"bib2","doi-asserted-by":"publisher","DOI":"10.1038\/srep17721","type":"journal-article","article-title":"Superdiffusive motion of membrane-targeting C2 domains","volume":"5","author":"Campagnola","year":"2015","journal-title":"Scientific Reports"},{"key":"bib3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2022.101868","type":"journal-article","article-title":"A genetically targeted sensor reveals spatial and temporal dynamics of acrosomal calcium and sperm acrosome exocytosis","volume":"298","author":"Cohen","year":"2022","journal-title":"The Journal of Biological Chemistry"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"681","DOI":"10.1530\/jrf.0.0820681","type":"journal-article","article-title":"Pre-loading of mouse oocytes with DNA-specific fluorochrome (Hoechst 33342) permits rapid detection of sperm-oocyte fusion","volume":"82","author":"Conover","year":"1988","journal-title":"Journal of Reproduction and Fertility"},{"key":"bib5","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.250654","type":"journal-article","article-title":"Long-term segmentation-free assessment of head-flagellum movement and intracellular calcium in swimming human sperm","volume":"134","author":"Corkidi","year":"2021","journal-title":"Journal of Cell Science"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"74","DOI":"10.1016\/j.biocel.2018.05.014","type":"journal-article","article-title":"SRRF: Universal live-cell super-resolution microscopy","volume":"101","author":"Culley","year":"2018","journal-title":"The International Journal of Biochemistry & Cell Biology"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1387\/ijdb.072550ad","type":"journal-article","article-title":"Sperm-activating peptides in the regulation of ion fluxes, signal transduction and motility","volume":"52","author":"Darszon","year":"2008","journal-title":"The International Journal of Developmental Biology"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"44384","DOI":"10.1074\/jbc.M112.393488","type":"journal-article","article-title":"Mouse sperm membrane potential hyperpolarization is necessary and sufficient to prepare sperm for the acrosome reaction","volume":"287","author":"De La Vega-Beltran","year":"2012","journal-title":"The Journal of Biological Chemistry"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"779","DOI":"10.1095\/biolreprod46.5.779","type":"journal-article","article-title":"Hyperactivated sperm progress in the mouse oviduct","volume":"46","author":"Demott","year":"1992","journal-title":"Biology of Reproduction"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"391","DOI":"10.1146\/annurev-cellbio-120219-021751","type":"journal-article","article-title":"The fertilization enigma: how sperm and egg fuse","volume":"37","author":"Deneke","year":"2021","journal-title":"Annual Review of Cell and Developmental Biology"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"2320","DOI":"10.1529\/biophysj.107.114645","type":"journal-article","article-title":"Mapping the number of molecules and brightness in the laser scanning microscope","volume":"94","author":"Digman","year":"2008","journal-title":"Biophysical Journal"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"C723","DOI":"10.1152\/ajpcell.00462.2010","type":"journal-article","article-title":"A practical guide to evaluating colocalization in biological microscopy","volume":"300","author":"Dunn","year":"2011","journal-title":"American Journal of Physiology. Cell Physiology"},{"key":"bib13","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/S0015-0282(16)48028-3","type":"journal-article","article-title":"In vitro fertilization in the rat: observations on living eggs","volume":"42","author":"Gaddum-Rosse","year":"1984","journal-title":"Fertility and Sterility"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1002\/mrd.1120060305","type":"journal-article","article-title":"Sperm tail entry into the mouse egg in vitro","volume":"6","author":"Gaddum\u2010Rosse","year":"1982","journal-title":"Gamete Research"},{"key":"bib15","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.215897","type":"journal-article","article-title":"The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structure","volume":"131","author":"Gervasi","year":"2018","journal-title":"Journal of Cell Science"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1016\/j.ydbio.2010.04.013","type":"journal-article","article-title":"Tuning sperm chemotaxis by calcium burst timing","volume":"344","author":"Guerrero","year":"2010","journal-title":"Developmental Biology"},{"key":"bib17","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12471","type":"journal-article","article-title":"Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations","volume":"7","author":"Gustafsson","year":"2016","journal-title":"Nature Communications"},{"key":"bib18","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1538\/expanim.59.105","type":"journal-article","article-title":"Transgenic mouse sperm that have green acrosome and red mitochondria allow visualization of sperm and their acrosome reaction in vivo","volume":"59","author":"Hasuwa","year":"2010","journal-title":"Experimental Animals"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1016\/0012-1606(86)90082-5","type":"journal-article","article-title":"Rapid visual detection of sperm-egg fusion using the DNA-specific fluorochrome Hoechst 33342","volume":"118","author":"Hinkley","year":"1986","journal-title":"Developmental Biology"},{"key":"bib20","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.116.140400","type":"journal-article","article-title":"The behavior and acrosomal status of mouse spermatozoa in vitro, and within the oviduct during fertilization after natural mating","volume":"95","author":"Hino","year":"2016","journal-title":"Biology of Reproduction"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"810","DOI":"10.1126\/science.1153529","type":"journal-article","article-title":"Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy","volume":"319","author":"Huang","year":"2008","journal-title":"Science"},{"key":"bib22","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1093\/jmicro\/dfy021","type":"journal-article","article-title":"Microscopic analysis of sperm movement: links to mechanisms and protein components","volume":"67","author":"Inaba","year":"2018","journal-title":"Microscopy"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1038\/nature03362","type":"journal-article","article-title":"The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs","volume":"434","author":"Inoue","year":"2005","journal-title":"Nature"},{"key":"bib24","doi-asserted-by":"publisher","first-page":"4957","DOI":"10.1364\/OE.20.004957","type":"journal-article","article-title":"PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking","volume":"20","author":"Izeddin","year":"2012","journal-title":"Optics Express"},{"key":"bib25","type":"software","article-title":"Reorganization-of-the-flagellum-scaffolding-induces-a-sperm-standstill-during-fertilization","volume-title":"Software Heritage","author":"Jablo\u00f1ski","year":"2024","unstructured":"Jablo\u00f1ski M. 2024. Reorganization-of-the-flagellum-scaffolding-induces-a-sperm-standstill-during-fertilization. Software Heritage. swh:1:rev:69acfc771171926a85ad6822f2e84d7e900cd5e0. https:\/\/archive.softwareheritage.org\/swh:1:dir:70fe35340d5a09c9aa6f7286bb881abf28413686;origin=https:\/\/github.com\/martijab\/Reorganization-of-the-Flagellum-Scaffolding-Induces-a-Sperm-Standstill-During-Fertilization;visit=swh:1:snp:efb0298508165591ebda705d372d933222c38062;anchor=swh:1:rev:69acfc771171926a85ad6822f2e84d7e900cd5e0."},{"key":"bib26","doi-asserted-by":"publisher","first-page":"4892","DOI":"10.1073\/pnas.1018202108","type":"journal-article","article-title":"Most fertilizing mouse spermatozoa begin their acrosome reaction before contact with the zona pellucida during in vitro fertilization","volume":"108","author":"Jin","year":"2011","journal-title":"PNAS"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"4627","DOI":"10.1242\/dev.125.23.4627","type":"journal-article","article-title":"The passage of Ca2+ and fluorescent markers between the sperm and egg after fusion in the mouse","volume":"125","author":"Jones","year":"1998","journal-title":"Development"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/j.ceb.2016.02.021","type":"journal-article","article-title":"Cortical actin and the plasma membrane: inextricably intertwined","volume":"38","author":"K\u00f6ster","year":"2016","journal-title":"Current Opinion in Cell Biology"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1016\/j.ydbio.2016.02.006","type":"journal-article","article-title":"Mouse sperm begin to undergo acrosomal exocytosis in the upper isthmus of the oviduct","volume":"411","author":"La Spina","year":"2016","journal-title":"Developmental Biology"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"760","DOI":"10.1038\/nmeth.4379","type":"journal-article","article-title":"sCMOS noise-correction algorithm for microscopy images","volume":"14","author":"Liu","year":"2017","journal-title":"Nature Methods"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1038\/nmeth.2972","type":"journal-article","article-title":"Fluorogenic probes for live-cell imaging of the cytoskeleton","volume":"11","author":"Lukinavi\u010dius","year":"2014","journal-title":"Nature Methods"},{"key":"bib32","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202002773RR","type":"journal-article","article-title":"Cdc42 localized in the CatSper signaling complex regulates cAMP-dependent pathways in mouse sperm","volume":"35","author":"Luque","year":"2021","journal-title":"FASEB Journal"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"111096","DOI":"10.18632\/oncotarget.22571","type":"journal-article","article-title":"Identifying the dynamics of actin and tubulin polymerization in iPSCs and in iPSC-derived neurons","volume":"8","author":"Magliocca","year":"2017","journal-title":"Oncotarget"},{"key":"bib34","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-13841-8","type":"journal-article","article-title":"Fast and accurate sCMOS noise correction for fluorescence microscopy","volume":"11","author":"Mandracchia","year":"2020","journal-title":"Nature Communications"},{"key":"bib35","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.115.135368","type":"journal-article","article-title":"Behavior of mouse spermatozoa in the female reproductive tract from soon after mating to the beginning of fertilization","volume":"94","author":"Muro","year":"2016","journal-title":"Biology of Reproduction"},{"key":"bib36","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1083\/jcb.66.2.263","type":"journal-article","article-title":"Ultrastructural localization of lectin-binding sites on the zonae pellucidae and plasma membranes of mammalian eggs","volume":"66","author":"Nicolson","year":"1975","journal-title":"The Journal of Cell Biology"},{"key":"bib37","doi-asserted-by":"publisher","first-page":"11493","DOI":"10.1073\/pnas.1922650117","type":"journal-article","article-title":"Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice","volume":"117","author":"Noda","year":"2020","journal-title":"PNAS"},{"key":"bib38","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1002\/ar.1092220106","type":"journal-article","article-title":"Development of mitochondrial helical sheath in the middle piece of the mouse spermatid tail: regular dispositions and synchronized changes","volume":"222","author":"Otani","year":"1988","journal-title":"The Anatomical Record"},{"key":"bib39","doi-asserted-by":"publisher","first-page":"2389","DOI":"10.1093\/bioinformatics\/btu202","type":"journal-article","article-title":"ThunderSTORM: a comprehensive ImageJ plug-in for PALM and STORM data analysis and super-resolution imaging","volume":"30","author":"Ovesn\u00fd","year":"2014","journal-title":"Bioinformatics"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"6151","DOI":"10.1073\/pnas.1018593108","type":"journal-article","article-title":"An actin cytoskeleton with evolutionarily conserved functions in the absence of canonical actin-binding proteins","volume":"108","author":"Paredez","year":"2011","journal-title":"PNAS"},{"key":"bib41","doi-asserted-by":"publisher","DOI":"10.1038\/srep31886","type":"journal-article","article-title":"A specific flagellum beating mode for inducing fusion in mammalian fertilization and kinetics of sperm internalization","volume":"6","author":"Ravaux","year":"2016","journal-title":"Scientific Reports"},{"key":"bib42","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.115.136085","type":"journal-article","article-title":"A specific transitory increase in intracellular calcium induced by progesterone promotes acrosomal exocytosis in mouse sperm","volume":"94","author":"Romarowski","year":"2016","journal-title":"Biology of Reproduction"},{"key":"bib43","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.114.119768","type":"journal-article","article-title":"Acrosome reaction and Ca2+ imaging in single human spermatozoa: New regulatory roles of [Ca2+]i","volume":"91","author":"S\u00e1nchez-C\u00e1rdenas","year":"2014","journal-title":"Biology of Reproduction"},{"key":"bib44","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202102146","type":"journal-article","article-title":"The cell biology of fertilization: Gamete attachment and fusion","volume":"220","author":"Siu","year":"2021","journal-title":"The Journal of Cell Biology"},{"key":"bib45","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1016\/0012-1606(88)90277-1","type":"journal-article","article-title":"A cell surface block to polyspermy occurs in golden hamster eggs","volume":"128","author":"Stewart-Savage","year":"1988","journal-title":"Developmental Biology"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"9435","DOI":"10.1074\/jbc.RA118.002286","type":"journal-article","article-title":"Disruption of protein kinase A localization induces acrosomal exocytosis in capacitated mouse sperm","volume":"293","author":"Stival","year":"2018","journal-title":"The Journal of Biological Chemistry"},{"key":"bib47","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-34693-9","type":"journal-article","article-title":"Extending resolution within a single imaging frame","volume":"13","author":"Torres-Garc\u00eda","year":"2022","journal-title":"Nature Communications"},{"key":"bib48","doi-asserted-by":"publisher","first-page":"6438","DOI":"10.1073\/pnas.1016325108","type":"journal-article","article-title":"Ergodic and nonergodic processes coexist in the plasma membrane as observed by single-molecule tracking","volume":"108","author":"Weigel","year":"2011","journal-title":"PNAS"},{"key":"bib49","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-28245-9","type":"journal-article","article-title":"Actin polymerization is activated by terahertz irradiation","volume":"8","author":"Yamazaki","year":"2018","journal-title":"Scientific Reports"},{"key":"bib50","volume-title":"Mammalian Fertilization","author":"Yanagimachi","year":"1994","type":"book"},{"key":"dataset1","doi-asserted-by":"publisher","DOI":"10.5281\/zenodo.13769608","type":"dataset","article-title":"Reorganization of the Flagellum Scaffolding Induces a Sperm Standstill During Fertilization","volume-title":"Zenodo","author":"Jablo\u00f1ski","year":"2024","unstructured":"Jablo\u00f1ski M, Luque G, G\u00f3mez-El\u00edas M, Sanchez-Cardenas C, Xu X, de la Vega-Beltran JL, Corkidi G, Linares A, Abonza Amaro VX, Arenas-Hernandez A, Ramos-Godinez MDP, L\u00f3pez-Saavedra A, Krapf D, Krapf D, Darszon A, Guerrero A, Buffone MG. 2024. Reorganization of the Flagellum Scaffolding Induces a Sperm Standstill During Fertilization. Zenodo."}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/93792\/elife-93792-v1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/93792\/elife-93792-v1.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/93792","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:08:57Z","timestamp":1787566137000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/93792"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,13]]},"references-count":51,"alternative-id":["10.7554\/eLife.93792"],"URL":"https:\/\/doi.org\/10.7554\/elife.93792","archive":["CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.06.22.546073","asserted-by":"subject"}],"is-same-as":[{"id-type":"doi","id":"10.7554\/eLife.93792.3","asserted-by":"subject"}],"is-version-of":[{"id-type":"doi","id":"10.7554\/eLife.93792.1","asserted-by":"subject"},{"id-type":"doi","id":"10.7554\/eLife.93792.2","asserted-by":"subject"}],"is-supplemented-by":[{"id-type":"doi","id":"10.5281\/zenodo.13769608","asserted-by":"subject"},{"id-type":"uri","id":"https:\/\/archive.softwareheritage.org\/swh:1:dir:70fe35340d5a09c9aa6f7286bb881abf28413686;origin=https:\/\/github.com\/martijab\/Reorganization-of-the-Flagellum-Scaffolding-Induces-a-Sperm-Standstill-During-Fertilization;visit=swh:1:snp:efb0298508165591ebda705d372d933222c38062;anchor=swh:1:rev:69acfc771171926a85ad6822f2e84d7e900cd5e0","asserted-by":"subject"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,13]]},"assertion":[{"value":"single anonymised","name":"peer_review_transparency","label":"Peer review transparency","group":{"name":"peer_review_taxonomy"}},{"value":"other reviewer(s), editor","name":"peer_review_interaction","label":"Peer review interaction","group":{"name":"peer_review_taxonomy"}},{"value":"review summaries, review reports, author\/editor communication, reviewer identities reviewer opt in, editor identities","name":"peer_review_published","label":"Peer review published","group":{"name":"peer_review_taxonomy"}},{"value":"open (sign in with ORCID iD required)","name":"post_publication_commenting","label":"Post publication commenting","group":{"name":"post_publication_commenting"}}],"article-number":"RP93792"}}