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The dedifferentiation of VSMCs is implicated in cerebrovascular disease and neurodegeneration. Despite its importance, the process of VSMC differentiation on brain arteries during development remains inadequately characterized. Understanding this process could aid in reprogramming and regenerating dedifferentiated VSMCs in cerebrovascular diseases. In this study, we investigated VSMC differentiation on zebrafish circle of Willis (CoW), comprising major arteries that supply blood to the vertebrate brain. We observed that arterial specification of CoW endothelial cells (ECs) occurs after their migration from cranial venous plexus to form CoW arteries. Subsequently,\n                    <jats:italic>acta2<\/jats:italic>\n                    + VSMCs differentiate from\n                    <jats:italic>pdgfrb<\/jats:italic>\n                    + mural cell progenitors after they were recruited to CoW arteries. The progression of VSMC differentiation exhibits a spatiotemporal pattern, advancing from anterior to posterior CoW arteries. Analysis of blood flow suggests that earlier VSMC differentiation in anterior CoW arteries correlates with higher red blood cell velocity and wall shear stress. Furthermore, pulsatile flow induces differentiation of human brain PDGFRB+ mural cells into VSMCs, and blood flow is required for VSMC differentiation on zebrafish CoW arteries. Consistently, flow-responsive transcription factor\n                    <jats:italic>klf2a<\/jats:italic>\n                    is activated in ECs of CoW arteries prior to VSMC differentiation, and\n                    <jats:italic>klf2a<\/jats:italic>\n                    knockdown delays VSMC differentiation on anterior CoW arteries. In summary, our findings highlight blood flow activation of endothelial\n                    <jats:italic>klf2a<\/jats:italic>\n                    as a mechanism regulating initial VSMC differentiation on vertebrate brain arteries.\n                  <\/jats:p>","DOI":"10.7554\/elife.94094","type":"journal-article","created":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T09:25:42Z","timestamp":1706865942000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.94094","source":"Crossref","is-referenced-by-count":8,"title":["Hemodynamics regulate spatiotemporal artery muscularization in the developing circle of Willis"],"prefix":"10.7554","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5013-7767","authenticated-orcid":true,"given":"Siyuan","family":"Cheng","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03v76x132","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Genetics, Yale School of Medicine","place":["New Haven, United 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