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Renal proximal tubular epithelial cells (RPTECs) and fibroblasts isolated from patients with cystinosis were transcriptionally profiled. Lysosomal fractionation, immunoblotting, confocal microscopy, intracellular pH, TEM, and mitochondrial stress\u202ftest were performed for validation. CRISPR,\n                    <jats:italic>CTNS<\/jats:italic>\n                    <jats:sup>-\/-<\/jats:sup>\n                    RPTECs were generated. Alterations in cell stress, pH, autophagic turnover, and mitochondrial energetics highlighted key changes in the V-ATPases in patient-derived and\n                    <jats:italic>CTNS<\/jats:italic>\n                    <jats:sup>-\/-<\/jats:sup>\n                    RPTECs. ATP6V0A1 was significantly downregulated in cystinosis and highly co-regulated with loss of\n                    <jats:italic>CTNS<\/jats:italic>\n                    . Correction of ATP6V0A1 rescued cell stress and mitochondrial function. Treatment of\n                    <jats:italic>CTNS<\/jats:italic>\n                    <jats:sup>-\/-<\/jats:sup>\n                    RPTECs with antioxidants ATX induced ATP6V0A1 expression and improved autophagosome turnover and mitochondrial integrity. Our exploratory transcriptional and in vitro cellular and functional studies confirm that loss of Cystinosin in RPTECs, results in a reduction in ATP6V0A1 expression, with changes in intracellular pH, mitochondrial integrity, mitochondrial function, and autophagosome-lysosome clearance. The novel findings are ATP6V0A1\u2019s role in cystinosis-associated renal pathology and among other antioxidants, ATX specifically upregulated ATP6V0A1, improved autophagosome turnover or reduced autophagy and mitochondrial integrity. This is a pilot study highlighting a novel mechanism of tubular injury in cystinosis.\n                  <\/jats:p>","DOI":"10.7554\/elife.94169","type":"journal-article","created":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T13:32:06Z","timestamp":1719581526000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.94169","source":"Crossref","is-referenced-by-count":1,"title":["Novel mechanism for tubular injury in nephropathic cystinosis"],"prefix":"10.7554","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9536-981X","authenticated-orcid":true,"given":"Swastika","family":"Sur","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/043mz5j54","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Multi-Organ Transplantation, Department of Surgery, University of California, San Francisco","place":["San Francisco, United 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