{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:41:12Z","timestamp":1787568072407,"version":"build-2736575974"},"reference-count":61,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T00:00:00Z","timestamp":1728000000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T00:00:00Z","timestamp":1728000000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T00:00:00Z","timestamp":1728000000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2018YFA0801004"],"award-info":[{"award-number":["2018YFA0801004"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870359"],"award-info":[{"award-number":["81870359"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Artificially sweetened beverages containing noncaloric monosaccharides were suggested as healthier alternatives to sugar-sweetened beverages. Nevertheless, the potential detrimental effects of these noncaloric monosaccharides on blood vessel function remain inadequately understood. We have established a zebrafish model that exhibits significant excessive angiogenesis induced by high glucose, resembling the hyperangiogenic characteristics observed in proliferative diabetic retinopathy (PDR). Utilizing this model, we observed that glucose and noncaloric monosaccharides could induce excessive formation of blood vessels, especially intersegmental vessels (ISVs). The excessively branched vessels were observed to be formed by ectopic activation of quiescent endothelial cells (ECs) into tip cells. Single-cell transcriptomic sequencing analysis of the ECs in the embryos exposed to high glucose revealed an augmented ratio of capillary ECs, proliferating ECs, and a series of upregulated proangiogenic genes. Further analysis and experiments validated that reduced\n                    <jats:italic>foxo1a<\/jats:italic>\n                    mediated the excessive angiogenesis induced by monosaccharides via upregulating the expression of\n                    <jats:italic>marcksl1a<\/jats:italic>\n                    . This study has provided new evidence showing the negative effects of noncaloric monosaccharides on the vascular system and the underlying mechanisms.\n                  <\/jats:p>","DOI":"10.7554\/elife.95427","type":"journal-article","created":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T11:31:46Z","timestamp":1712143906000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.95427","source":"Crossref","is-referenced-by-count":2,"title":["Noncaloric monosaccharides induce excessive sprouting angiogenesis in zebrafish via foxo1a-marcksl1a signal"],"prefix":"10.7554","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0222-0755","authenticated-orcid":true,"given":"Xiaoning","family":"Wang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02afcvw97","id-type":"ROR","asserted-by":"publisher"}],"name":"Affiliated Hospital of Nantong University, Nantong Laboratory of Development and Diseases, School of Life Science; Co-innovation Center of Neuroregeneration, Nantong University","place":["Nantong, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"given":"Jinxiang","family":"Zhao","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02afcvw97","id-type":"ROR","asserted-by":"publisher"}],"name":"Affiliated Hospital of Nantong University, Nantong Laboratory of Development and Diseases, School of Life Science; Co-innovation Center of Neuroregeneration, Nantong University","place":["Nantong, China"]},{"name":"Suqian First Hospital","place":["Suqian, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Jiehuan","family":"Xu","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02afcvw97","id-type":"ROR","asserted-by":"publisher"}],"name":"Affiliated Hospital of Nantong University, Nantong Laboratory of Development and Diseases, School of Life Science; Co-innovation Center of Neuroregeneration, Nantong University","place":["Nantong, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"}]},{"given":"Bowen","family":"Li","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02afcvw97","id-type":"ROR","asserted-by":"publisher"}],"name":"Medical School, Nantong University","place":["Nantong, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"resources","vocabulary":"credit"},{"role":"software","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5473-5596","authenticated-orcid":true,"given":"Xia","family":"Liu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02afcvw97","id-type":"ROR","asserted-by":"publisher"}],"name":"Affiliated Hospital of Nantong University, Nantong Laboratory of Development and Diseases, School of Life Science; Co-innovation Center of Neuroregeneration, Nantong University","place":["Nantong, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"corresponding-author","vocabulary":"crossref"},{"role":"project-administration","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8286-2987","authenticated-orcid":true,"given":"Gangcai","family":"Xie","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02afcvw97","id-type":"ROR","asserted-by":"publisher"}],"name":"Medical School, Nantong University","place":["Nantong, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"corresponding-author","vocabulary":"crossref"},{"role":"project-administration","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"software","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4763-126X","authenticated-orcid":true,"given":"Xuchu","family":"Duan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02afcvw97","id-type":"ROR","asserted-by":"publisher"}],"name":"Affiliated Hospital of Nantong University, Nantong Laboratory of Development and Diseases, School of Life Science; Co-innovation Center of Neuroregeneration, Nantong University","place":["Nantong, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"corresponding-author","vocabulary":"crossref"},{"role":"project-administration","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2764-6544","authenticated-orcid":true,"given":"Dong","family":"Liu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02afcvw97","id-type":"ROR","asserted-by":"publisher"}],"name":"Affiliated Hospital of Nantong University, Nantong Laboratory of Development and Diseases, School of Life Science; Co-innovation Center of Neuroregeneration, Nantong University","place":["Nantong, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"corresponding-author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"4374","published-online":{"date-parts":[[2024,10,4]]},"reference":[{"key":"bib1","type":"software","article-title":"Cell ranger","volume-title":"Genomics","author":"10xGenomics Company","year":"2019","unstructured":"10xGenomics Company. 2019. Cell ranger. Genomics. 3.0.2. https:\/\/www.10xgenomics.com\/support\/software\/cell-ranger."},{"key":"bib2","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1242\/dmm.003772","type":"journal-article","article-title":"Predominant cone photoreceptor dysfunction in a hyperglycaemic model of non-proliferative diabetic retinopathy","volume":"3","author":"Alvarez","year":"2010","journal-title":"Disease Models & Mechanisms"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1038\/s41556-021-00637-6","type":"journal-article","article-title":"Control of endothelial quiescence by FOXO-regulated metabolites","volume":"23","author":"Andrade","year":"2021","journal-title":"Nature Cell Biology"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"1400","DOI":"10.1038\/bjp.2008.249","type":"journal-article","article-title":"Zebrafish: An emerging technology for in vivo pharmacological assessment to identify potential safety liabilities in early drug discovery","volume":"154","author":"Barros","year":"2008","journal-title":"British Journal of Pharmacology"},{"key":"bib5","first-page":"3584","type":"journal-article","article-title":"Mechanistic insights into the role of FOXO in diabetic retinopathy","volume":"14","author":"Behl","year":"2022","journal-title":"American Journal of Translational Research"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"1092","DOI":"10.1016\/s0140-6736(86)91352-8","type":"journal-article","article-title":"Paradoxical effects of an intense sweetener (aspartame) on appetite","volume":"1","author":"Blundell","year":"1986","journal-title":"Lancet"},{"key":"bib7","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-023-03893-2","type":"journal-article","article-title":"The link between glycemic control measures and eye microvascular complications in a clinical cohort of type 2 diabetes with microRNA-223-3p signature","volume":"21","author":"Da\u2019as","year":"2023","journal-title":"Journal of Translational Medicine"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"1321","DOI":"10.3945\/ajcn.110.007922","type":"journal-article","article-title":"Sugar-sweetened and artificially sweetened beverage consumption and risk of type 2 diabetes in men","volume":"93","author":"de Koning","year":"2011","journal-title":"The American Journal of Clinical Nutrition"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"1735","DOI":"10.1161\/CIRCULATIONAHA.111.067017","type":"journal-article","article-title":"Sweetened beverage consumption, incident coronary heart disease, and biomarkers of risk in men","volume":"125","author":"de Koning","year":"2012","journal-title":"Circulation"},{"key":"bib10","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/653789","type":"journal-article","article-title":"Impact of diabetes on cardiovascular disease: an update","volume":"2013","author":"de Matheus","year":"2013","journal-title":"International Journal of Hypertension"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"2181","DOI":"10.2337\/dc19-0734","type":"journal-article","article-title":"Changes in consumption of sugary beverages and artificially sweetened beverages and subsequent risk of type 2 diabetes: Results from three large prospective U.S. cohorts of women and men","volume":"42","author":"Drouin-Chartier","year":"2019","journal-title":"Diabetes Care"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1677\/JME-06-0037","type":"journal-article","article-title":"Larval zebrafish as a model for glucose metabolism: expression of phosphoenolpyruvate carboxykinase as a marker for exposure to anti-diabetic compounds","volume":"38","author":"Elo","year":"2007","journal-title":"Journal of Molecular Endocrinology"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"517","DOI":"10.3945\/ajcn.112.050997","type":"journal-article","article-title":"Consumption of artificially and sugar-sweetened beverages and incident type 2 diabetes in the Etude Epidemiologique aupres des femmes de la Mutuelle Generale de l\u2019Education Nationale-European Prospective Investigation into Cancer and Nutrition cohort","volume":"97","author":"Fagherazzi","year":"2013","journal-title":"The American Journal of Clinical Nutrition"},{"key":"bib14","first-page":"1","type":"journal-article","article-title":"Consumption of diet drinks in the United States, 2009\u20122010","volume":"1v","author":"Fakhouri","year":"2012","journal-title":"NCHS Data Brief"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"753","DOI":"10.1161\/CIRCULATIONAHA.112.093245","type":"journal-article","article-title":"The assessment of endothelial function: From research into clinical practice","volume":"126","author":"Flammer","year":"2012","journal-title":"Circulation"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"2495","DOI":"10.1001\/jama.292.20.2495","type":"journal-article","article-title":"Trends in cardiovascular complications of diabetes","volume":"292","author":"Fox","year":"2004","journal-title":"JAMA"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"1037","DOI":"10.3945\/ajcn.2008.27140","type":"journal-article","article-title":"Sweetened beverage consumption and risk of coronary heart disease in women","volume":"89","author":"Fung","year":"2009","journal-title":"The American Journal of Clinical Nutrition"},{"key":"bib18","doi-asserted-by":"publisher","first-page":"1120","DOI":"10.1007\/s11606-011-1968-2","type":"journal-article","article-title":"Diet soft drink consumption is associated with an increased risk of vascular events in the Northern Manhattan Study","volume":"27","author":"Gardener","year":"2012","journal-title":"Journal of General Internal Medicine"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1007\/s00592-007-0257-3","type":"journal-article","article-title":"Induction of hyperglycaemia in zebrafish (Danio rerio) leads to morphological changes in the retina","volume":"44","author":"Gleeson","year":"2007","journal-title":"Acta Diabetologica"},{"key":"bib20","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1874-1","type":"journal-article","article-title":"Normalization and variance stabilization of single-cell RNA-seq data using regularized negative binomial regression","volume":"20","author":"Hafemeister","year":"2019","journal-title":"Genome Biology"},{"key":"bib21","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18092002","type":"journal-article","article-title":"Zebrafish as a model for the study of microvascular complications of diabetes and their mechanisms","volume":"18","author":"Heckler","year":"2017","journal-title":"International Journal of Molecular Sciences"},{"key":"bib22","doi-asserted-by":"publisher","first-page":"733","DOI":"10.1093\/ajcn\/nqz154","type":"journal-article","article-title":"Cumulative intake of artificially sweetened and sugar-sweetened beverages and risk of incident type 2 diabetes in young adults: the Coronary Artery Risk Development In Young Adults (CARDIA) Study","volume":"110","author":"Hirahatake","year":"2019","journal-title":"The American Journal of Clinical Nutrition"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1016\/j.gep.2013.04.005","type":"journal-article","article-title":"Nonmuscle myosin II-B (myh10) expression analysis during zebrafish embryonic development","volume":"13","author":"Huang","year":"2013","journal-title":"Gene Expression Patterns"},{"key":"bib24","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.h3576","type":"journal-article","article-title":"Consumption of sugar sweetened beverages, artificially sweetened beverages, and fruit juice and incidence of type 2 diabetes: systematic review, meta-analysis, and estimation of population attributable fraction","volume":"351","author":"Imamura","year":"2015","journal-title":"BMJ"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1055\/s-0032-1304565","type":"journal-article","article-title":"Zebrafish: A model for understanding diabetic complications","volume":"120","author":"J\u00f6rgens","year":"2012","journal-title":"Experimental and Clinical Endocrinology & Diabetes"},{"key":"bib26","doi-asserted-by":"publisher","first-page":"213","DOI":"10.2337\/db14-0352","type":"journal-article","article-title":"High tissue glucose alters intersomitic blood vessels in zebrafish via methylglyoxal targeting the VEGF receptor signaling cascade","volume":"64","author":"J\u00f6rgens","year":"2015","journal-title":"Diabetes"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1111\/bph.13279","type":"journal-article","article-title":"High glucose-induced changes in hyaloid-retinal vessels during early ocular development of zebrafish: a short-term animal model of diabetic retinopathy","volume":"173","author":"Jung","year":"2016","journal-title":"British Journal of Pharmacology"},{"key":"bib28","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/918267","type":"journal-article","article-title":"Endothelial dysfunction and diabetes: Effects on angiogenesis, vascular remodeling, and wound healing","volume":"2012","author":"Kolluru","year":"2012","journal-title":"International Journal of Vascular Medicine"},{"key":"bib29","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-19308-5","type":"journal-article","article-title":"Marcksl1 modulates endothelial cell mechanoresponse to haemodynamic forces to control blood vessel shape and size","volume":"11","author":"Kondrychyn","year":"2020","journal-title":"Nature Communications"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"2111","DOI":"10.1242\/dev.063933","type":"journal-article","article-title":"Flt1 acts as a negative regulator of tip cell formation and branching morphogenesis in the zebrafish embryo","volume":"138","author":"Krueger","year":"2011","journal-title":"Development"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"3088","DOI":"10.1002\/dvdy.21343","type":"journal-article","article-title":"The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs","volume":"236","author":"Kwan","year":"2007","journal-title":"Developmental Dynamics"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"856","DOI":"10.3945\/jn.114.190546","type":"journal-article","article-title":"Sweetened beverage consumption is associated with increased risk of stroke in women and men","volume":"144","author":"Larsson","year":"2014","journal-title":"The Journal of Nutrition"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1111\/j.1467-789X.2008.00562.x","type":"journal-article","article-title":"Diabetes, insulin resistance and sugars","volume":"10 Suppl 1","author":"Laville","year":"2009","journal-title":"Obesity Reviews"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"572","DOI":"10.1097\/HCO.0000000000000439","type":"journal-article","article-title":"Sugar sweetened beverages and cardiometabolic health","volume":"32","author":"Malik","year":"2017","journal-title":"Current Opinion in Cardiology"},{"key":"bib35","doi-asserted-by":"publisher","first-page":"1447","DOI":"10.1093\/jn\/137.6.1447","type":"journal-article","article-title":"Consumption of sweetened beverages and intakes of fructose and glucose predict type 2 diabetes occurrence","volume":"137","author":"Montonen","year":"2007","journal-title":"The Journal of Nutrition"},{"key":"bib36","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2020.00444","type":"journal-article","article-title":"Neuroendocrine and metabolic effects of low-calorie and non-calorie sweeteners","volume":"11","author":"Moriconi","year":"2020","journal-title":"Frontiers in Endocrinology"},{"key":"bib37","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1161\/STROKEAHA.118.023100","type":"journal-article","article-title":"Artificially sweetened beverages and stroke, coronary heart disease, and all-cause mortality in the women\u2019s health initiative","volume":"50","author":"Mossavar-Rahmani","year":"2019","journal-title":"Stroke"},{"key":"bib38","doi-asserted-by":"publisher","first-page":"791","DOI":"10.1111\/ijcp.12841","type":"journal-article","article-title":"Soft drinks and sweetened beverages and the risk of cardiovascular disease and mortality: A systematic review and meta-analysis","volume":"70","author":"Narain","year":"2016","journal-title":"International Journal of Clinical Practice"},{"key":"bib39","doi-asserted-by":"publisher","first-page":"488","DOI":"10.1016\/j.physbeh.2016.04.029","type":"journal-article","article-title":"Reshaping the gut microbiota: Impact of low calorie sweeteners and the link to insulin resistance?","volume":"164","author":"Nettleton","year":"2016","journal-title":"Physiology & Behavior"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1016\/s0891-6632(87)80069-7","type":"journal-article","article-title":"New vessel formation in the renal corpuscles in advanced diabetic glomerulopathy","volume":"1","author":"Osterby","year":"1987","journal-title":"The Journal of Diabetic Complications"},{"key":"bib41","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2023.176089","type":"journal-article","article-title":"Emerging links between FOXOs and diabetic complications","volume":"960","author":"Parmar","year":"2023","journal-title":"European Journal of Pharmacology"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"450","DOI":"10.1016\/j.physbeh.2015.06.024","type":"journal-article","article-title":"Metabolic effects of non-nutritive sweeteners","volume":"152","author":"Pepino","year":"2015","journal-title":"Physiology & Behavior"},{"key":"bib43","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201901082","type":"journal-article","article-title":"Lactose, maltose, and sucrose in health and disease","volume":"64","author":"Qi","year":"2020","journal-title":"Molecular Nutrition & Food Research"},{"key":"bib44","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/j.cmet.2012.11.012","type":"journal-article","article-title":"Vascular complications of diabetes: Mechanisms of injury and protective factors","volume":"17","author":"Rask-Madsen","year":"2013","journal-title":"Cell Metabolism"},{"key":"bib45","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.39780","type":"journal-article","article-title":"Endothelial-specific FoxO1 depletion prevents obesity-related disorders by increasing vascular metabolism and growth","volume":"7","author":"Rudnicki","year":"2018","journal-title":"eLife"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"1888","DOI":"10.1016\/j.cell.2019.05.031","type":"journal-article","article-title":"Comprehensive integration of single-cell data","volume":"177","author":"Stuart","year":"2019","journal-title":"Cell"},{"key":"bib47","doi-asserted-by":"publisher","first-page":"640","DOI":"10.3945\/ajcn.112.034751","type":"journal-article","article-title":"Low-calorie sweetener consumption is increasing in the United States","volume":"96","author":"Sylvetsky","year":"2012","journal-title":"The American Journal of Clinical Nutrition"},{"key":"bib48","doi-asserted-by":"publisher","first-page":"929","DOI":"10.1172\/JCI114521","type":"journal-article","article-title":"Elevated glucose promotes generation of endothelium-derived vasoconstrictor prostanoids in rabbit aorta","volume":"85","author":"Tesfamariam","year":"1990","journal-title":"The Journal of Clinical Investigation"},{"key":"bib49","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1172\/JCI118394","type":"journal-article","article-title":"Vitamin C improves endothelium-dependent vasodilation in patients with non-insulin-dependent diabetes mellitus","volume":"97","author":"Ting","year":"1996","journal-title":"The Journal of Clinical Investigation"},{"key":"bib50","doi-asserted-by":"publisher","first-page":"3077","DOI":"10.1002\/dvdy.21354","type":"journal-article","article-title":"Gateway compatible vectors for analysis of gene function in the zebrafish","volume":"236","author":"Villefranc","year":"2007","journal-title":"Developmental Dynamics"},{"key":"bib51","doi-asserted-by":"publisher","first-page":"462","DOI":"10.1007\/s11606-014-3098-0","type":"journal-article","article-title":"Diet drink consumption and the risk of cardiovascular events: A report from the Women\u2019s health initiative","volume":"30","author":"Vyas","year":"2015","journal-title":"Journal of General Internal Medicine"},{"key":"bib52","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1093\/cvr\/cvw023","type":"journal-article","article-title":"MicroRNA-10a\/10b represses a novel target gene mib1 to regulate angiogenesis","volume":"110","author":"Wang","year":"2016","journal-title":"Cardiovascular Research"},{"key":"bib53","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1038\/nature16498","type":"journal-article","article-title":"FOXO1 couples metabolic activity and growth state in the vascular endothelium","volume":"529","author":"Wilhelm","year":"2016","journal-title":"Nature"},{"key":"bib54","doi-asserted-by":"publisher","first-page":"3331","DOI":"10.2174\/1381612043383142","type":"journal-article","article-title":"Vasoactive factors and diabetic retinopathy: Vascular endothelial growth factor, cycoloxygenase-2 and nitric oxide","volume":"10","author":"Wilkinson-Berka","year":"2004","journal-title":"Current Pharmaceutical Design"},{"key":"bib55","type":"software","article-title":"ZebEndoimmune","volume-title":"Github","author":"Xie","year":"2024","unstructured":"Xie G. 2024. ZebEndoimmune. Github. 0.1.0. https:\/\/github.com\/gangcai\/ZebEndoimmune."},{"key":"bib56","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1089\/omi.2011.0118","type":"journal-article","article-title":"clusterProfiler: an R package for comparing biological themes among gene clusters","volume":"16","author":"Yu","year":"2012","journal-title":"Omics"},{"key":"bib57","type":"software","article-title":"ClusterProfiler","volume-title":"Bioconductor","author":"Yu","year":"2019","unstructured":"Yu G, Wang LG, Han Y, He QY. 2019. ClusterProfiler. Bioconductor. 3.14.3. https:\/\/www.bioconductor.org\/packages\/3.10\/bioc\/html\/clusterProfiler.html."},{"key":"bib58","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2018.00091","type":"journal-article","article-title":"Zebrafish as a Model for Obesity and Diabetes","volume":"6","author":"Zang","year":"2018","journal-title":"Frontiers in Cell and Developmental Biology"},{"key":"bib59","doi-asserted-by":"publisher","first-page":"895","DOI":"10.1038\/s41422-019-0229-5","type":"journal-article","article-title":"Endothelial CDS2 deficiency causes VEGFA-mediated vascular regression and tumor inhibition","volume":"29","author":"Zhao","year":"2019","journal-title":"Cell Research"},{"key":"dataset1","type":"dataset","article-title":"Single-cell transcriptome sequencing analysis of endothelial cells in control and high glucose treated zebrafish embryos","volume-title":"NCBI Gene Expression Omnibus","author":"Wang","year":"2024","unstructured":"Wang X, Zhao J, Xu J, Li B, Liu X, Xie G, Duan X, Liu D. 2024. Single-cell transcriptome sequencing analysis of endothelial cells in control and high glucose treated zebrafish embryos. NCBI Gene Expression Omnibus. https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE276251. GSE276251."},{"key":"dataset2","type":"dataset","article-title":"Effect of D-glucose and L-glucose on gene expression of zebrafish embryos at 72 hpf (hours post fertilization) and 96 hpf","volume-title":"NCBI Gene Expression Omnibus","author":"Wang","year":"2024","unstructured":"Wang X, Zhao J, Xu J, Li B, Liu X, Xie G, Duan X, Liu D. 2024. Effect of D-glucose and L-glucose on gene expression of zebrafish embryos at 72 hpf (hours post fertilization) and 96 hpf. NCBI Gene Expression Omnibus. https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE276252. GSE276252."}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/95427\/elife-95427-v1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/95427\/elife-95427-v1.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/95427","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:11:42Z","timestamp":1787566302000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/95427"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,4]]},"references-count":61,"alternative-id":["10.7554\/eLife.95427"],"URL":"https:\/\/doi.org\/10.7554\/elife.95427","archive":["CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.01.18.576182","asserted-by":"subject"},{"id-type":"doi","id":"10.1101\/2024.01.18.576182","asserted-by":"object"}],"is-same-as":[{"id-type":"doi","id":"10.7554\/eLife.95427.3","asserted-by":"subject"}],"is-version-of":[{"id-type":"doi","id":"10.7554\/eLife.95427.1","asserted-by":"subject"},{"id-type":"doi","id":"10.7554\/eLife.95427.2","asserted-by":"subject"}],"is-supplemented-by":[{"id-type":"accession","id":"GSE276251","asserted-by":"subject"},{"id-type":"accession","id":"GSE276252","asserted-by":"subject"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,4]]},"assertion":[{"value":"single anonymised","name":"peer_review_transparency","label":"Peer review transparency","group":{"name":"peer_review_taxonomy"}},{"value":"other reviewer(s), editor","name":"peer_review_interaction","label":"Peer review interaction","group":{"name":"peer_review_taxonomy"}},{"value":"review summaries, review reports, author\/editor communication, reviewer identities reviewer opt in, editor identities","name":"peer_review_published","label":"Peer review published","group":{"name":"peer_review_taxonomy"}},{"value":"open (sign in with ORCID iD required)","name":"post_publication_commenting","label":"Post publication commenting","group":{"name":"post_publication_commenting"}}],"article-number":"RP95427"}}