{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:42:41Z","timestamp":1787568161747,"version":"build-2736575974"},"reference-count":85,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T00:00:00Z","timestamp":1725926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T00:00:00Z","timestamp":1725926400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T00:00:00Z","timestamp":1725926400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000052","name":"NIH Office of the Director","doi-asserted-by":"publisher","award":["R24OD010943"],"award-info":[{"award-number":["R24OD010943"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["5R01GM135326"],"award-info":[{"award-number":["5R01GM135326"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["R37AG56510"],"award-info":[{"award-number":["R37AG56510"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["R01AG047101"],"award-info":[{"award-number":["R01AG047101"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["K12GM093854"],"award-info":[{"award-number":["K12GM093854"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Large vesicle extrusion from neurons may contribute to spreading pathogenic protein aggregates and promoting inflammatory responses, two mechanisms leading to neurodegenerative disease. Factors that regulate the extrusion of large vesicles, such as exophers produced by proteostressed\n                    <jats:italic>C. elegans<\/jats:italic>\n                    touch neurons, are poorly understood. Here, we document that mechanical force can significantly potentiate exopher extrusion from proteostressed neurons. Exopher production from the\n                    <jats:italic>C. elegans<\/jats:italic>\n                    ALMR neuron peaks at adult day 2 or 3, coinciding with the\n                    <jats:italic>C. elegans<\/jats:italic>\n                    reproductive peak. Genetic disruption of\n                    <jats:italic>C. elegans<\/jats:italic>\n                    germline, sperm, oocytes, or egg\/early embryo production can strongly suppress exopher extrusion from the ALMR neurons during the peak period. Conversely, restoring egg production at the late reproductive phase through mating with males or inducing egg retention via genetic interventions that block egg-laying can strongly increase ALMR exopher production. Overall, genetic interventions that promote ALMR exopher production are associated with expanded uterus lengths and genetic interventions that suppress ALMR exopher production are associated with shorter uterus lengths. In addition to the impact of fertilized eggs, ALMR exopher production can be enhanced by filling the uterus with oocytes, dead eggs, or even fluid, supporting that distention consequences, rather than the presence of fertilized eggs, constitute the exopher-inducing stimulus. We conclude that the mechanical force of uterine occupation potentiates exopher extrusion from proximal proteostressed maternal neurons. Our observations draw attention to the potential importance of mechanical signaling in extracellular vesicle production and in aggregate spreading mechanisms, making a case for enhanced attention to mechanobiology in neurodegenerative disease.\n                  <\/jats:p>","DOI":"10.7554\/elife.95443","type":"journal-article","created":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T10:25:45Z","timestamp":1708338345000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.95443","source":"Crossref","is-referenced-by-count":3,"title":["Mechanical force of uterine occupation enables large vesicle extrusion from proteostressed maternal neurons"],"prefix":"10.7554","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3694-7103","authenticated-orcid":true,"given":"Guoqiang","family":"Wang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05vt9qd57","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology and Biochemistry, Nelson Biological Laboratories, Rutgers, The State University of New Jersey","place":["Piscataway, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Ryan J","family":"Guasp","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05vt9qd57","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology and Biochemistry, Nelson Biological Laboratories, Rutgers, The State University of New Jersey","place":["Piscataway, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Sangeena","family":"Salam","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05vt9qd57","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology and Biochemistry, Nelson Biological Laboratories, Rutgers, The State University of New Jersey","place":["Piscataway, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Edward","family":"Chuang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05vt9qd57","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology and Biochemistry, Nelson Biological Laboratories, Rutgers, The State University of New Jersey","place":["Piscataway, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"given":"Andr\u00e9s","family":"Morera","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05vt9qd57","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology and Biochemistry, Nelson Biological Laboratories, Rutgers, The State University of New Jersey","place":["Piscataway, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Anna J","family":"Smart","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05vt9qd57","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology and Biochemistry, Nelson Biological Laboratories, Rutgers, The State University of New Jersey","place":["Piscataway, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"David","family":"Jimenez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05vt9qd57","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology and Biochemistry, Nelson Biological Laboratories, Rutgers, The State University of New Jersey","place":["Piscataway, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Sahana","family":"Shekhar","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05vt9qd57","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology and Biochemistry, Nelson Biological Laboratories, Rutgers, The State University of New Jersey","place":["Piscataway, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Emily","family":"Friedman","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05vt9qd57","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology and Biochemistry, Nelson Biological Laboratories, Rutgers, The State University of New Jersey","place":["Piscataway, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Ilija","family":"Melentijevic","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05vt9qd57","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology and Biochemistry, Nelson Biological Laboratories, Rutgers, The State University of New Jersey","place":["Piscataway, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"}]},{"given":"Ken C","family":"Nguyen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05cf8a891","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Neuroscience, Albert Einstein College of Medicine","place":["Bronx, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"}]},{"given":"David H","family":"Hall","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05cf8a891","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Neuroscience, Albert Einstein College of Medicine","place":["Bronx, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"data-curation","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5943-8336","authenticated-orcid":true,"given":"Barth D","family":"Grant","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05vt9qd57","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology and Biochemistry, Nelson Biological Laboratories, Rutgers, The State University of New Jersey","place":["Piscataway, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8751-7429","authenticated-orcid":true,"given":"Monica","family":"Driscoll","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05vt9qd57","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology and Biochemistry, Nelson Biological Laboratories, Rutgers, The State University of New Jersey","place":["Piscataway, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"corresponding-author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"4374","published-online":{"date-parts":[[2024,9,10]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1038\/349346a0","type":"journal-article","article-title":"Control of the sperm-oocyte switch in Caenorhabditis elegans hermaphrodites by the fem-3 3\u2019 untranslated region","volume":"349","author":"Ahringer","year":"1991","journal-title":"Nature"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"759","DOI":"10.18632\/aging.100605","type":"journal-article","article-title":"A DNA synthesis inhibitor is protective against proteotoxic stressors via modulation of fertility pathways in Caenorhabditis elegans","volume":"5","author":"Angeli","year":"2013","journal-title":"Aging"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3791\/61368","type":"journal-article","article-title":"Quantitative approaches for scoring in vivo neuronal aggregate and organelle extrusion in large exopher vesicles in C. elegans","volume":"1","author":"Arnold","year":"2020","journal-title":"Journal of Visualized Experiments"},{"key":"bib4","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-39700-1","type":"journal-article","article-title":"Intermediate filaments associate with aggresome-like structures in proteostressed C. elegans neurons and influence large vesicle extrusions as exophers","volume":"14","author":"Arnold","year":"2023","journal-title":"Nature Communications"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1242\/dev.116.3.755","type":"journal-article","article-title":"Characterization of a germ-line proliferation mutation in C. elegans","volume":"116","author":"Beanan","year":"1992","journal-title":"Development"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"382","DOI":"10.1016\/j.mod.2009.02.001","type":"journal-article","article-title":"Two cytochrome P450s in Caenorhabditis elegans are essential for the organization of eggshell, correct execution of meiosis and the polarization of embryo","volume":"126","author":"Benenati","year":"2009","journal-title":"Mechanisms of Development"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1038\/nature24620","type":"journal-article","article-title":"A lysosomal switch triggers proteostasis renewal in the immortal C. elegans germ lineage","volume":"551","author":"Bohnert","year":"2017","journal-title":"Nature"},{"key":"bib8","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.36194","type":"journal-article","article-title":"Reactivation of RNA metabolism underlies somatic restoration after adult reproductive diapause in C. elegans","volume":"7","author":"Burnaevskiy","year":"2018","journal-title":"eLife"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"358","DOI":"10.1016\/0012-1606(81)90459-0","type":"journal-article","article-title":"Developmental genetics of the mechanosensory neurons of Caenorhabditis elegans","volume":"82","author":"Chalfie","year":"1981","journal-title":"Developmental Biology"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1895\/wormbook.1.132.1","type":"journal-article","article-title":"Biogenic amine neurotransmitters in C. elegans","volume":"1","author":"Chase","year":"2007","journal-title":"WormBook"},{"key":"bib11","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2101410118","type":"journal-article","article-title":"Stress increases in exopher-mediated neuronal extrusion require lipid biosynthesis, FGF, and EGF RAS\/MAPK signaling","volume":"118","author":"Cooper","year":"2021","journal-title":"PNAS"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1895\/wormbook.1.177.1","type":"journal-article","article-title":"A Transparent window into biology: A primer on Caenorhabditis elegans","volume":"1","author":"Corsi","year":"2015","journal-title":"WormBook"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"9633","DOI":"10.1073\/pnas.1404651111","type":"journal-article","article-title":"Transcellular degradation of axonal mitochondria","volume":"111","author":"Davis","year":"2014","journal-title":"PNAS"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1146\/annurev-cellbio-100617-062636","type":"journal-article","article-title":"Intercellular spread of protein aggregates in neurodegenerative disease","volume":"34","author":"Davis","year":"2018","journal-title":"Annual Review of Cell and Developmental Biology"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1016\/j.cell.2013.09.026","type":"journal-article","article-title":"Mechano-gated ion channels in sensory systems","volume":"155","author":"Delmas","year":"2013","journal-title":"Cell"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1016\/0012-1606(84)90077-0","type":"journal-article","article-title":"A sex-determining gene, fem-1, required for both male and hermaphrodite development in Caenorhabditis elegans","volume":"106","author":"Doniach","year":"1984","journal-title":"Developmental Biology"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1895\/wormbook.1.82.2","type":"journal-article","article-title":"Sex determination in the germ line","volume":"1","author":"Ellis","year":"2007","journal-title":"WormBook"},{"key":"bib18","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-20117-6","type":"journal-article","article-title":"Pulsatile stretch as a novel modulator of amyloid precursor protein processing and associated inflammatory markers in human cerebral endothelial cells","volume":"8","author":"Gangoda","year":"2018","journal-title":"Scientific Reports"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"288","DOI":"10.1016\/j.ydbio.2018.08.005","type":"journal-article","article-title":"CBD-1 organizes two independent complexes required for eggshell vitelline layer formation and egg activation in C. elegans","volume":"442","author":"Gonz\u00e1lez","year":"2018","journal-title":"Developmental Biology"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1895\/wormbook.1.62.1","type":"journal-article","article-title":"Mechanosensation","volume":"1","author":"Goodman","year":"2006","journal-title":"WormBook"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"522","DOI":"10.1016\/j.celrep.2014.09.033","type":"journal-article","article-title":"Mechanosensitive neurons on the internal reproductive tract contribute to egg-laying-induced acetic acid attraction in Drosophila","volume":"9","author":"Gou","year":"2014","journal-title":"Cell Reports"},{"key":"bib22","doi-asserted-by":"publisher","first-page":"1257","DOI":"10.1016\/j.cub.2006.05.020","type":"journal-article","article-title":"Galphao\/i and Galphas signaling function in parallel with the MSP\/Eph receptor to control meiotic diapause in C. elegans","volume":"16","author":"Govindan","year":"2006","journal-title":"Current Biology"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1895\/wormbook.1.53.1","type":"journal-article","article-title":"Control of oocyte meiotic maturation and fertilization","volume":"1","author":"Greenstein","year":"2005","journal-title":"WormBook"},{"key":"bib24","doi-asserted-by":"publisher","first-page":"3851","DOI":"10.1111\/ejn.14766","type":"journal-article","article-title":"Mechanobiology of the brain in ageing and Alzheimer\u2019s disease","volume":"53","author":"Hall","year":"2021","journal-title":"The European Journal of Neuroscience"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"1012","DOI":"10.1126\/science.6805073","type":"journal-article","article-title":"Serotonin and octopamine in the nematode Caenorhabditis elegans","volume":"216","author":"Horvitz","year":"1982","journal-title":"Science"},{"key":"bib26","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/j.neuron.2022.10.021","type":"journal-article","article-title":"Microglial Piezo1 senses A\u03b2 fibril stiffness to restrict Alzheimer\u2019s disease","volume":"111","author":"Hu","year":"2023","journal-title":"Neuron"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1038\/s41565-023-01366-7","type":"journal-article","article-title":"Proximal tubules eliminate endocytosed gold nanoparticles through an organelle-extrusion-mediated self-renewal mechanism","volume":"18","author":"Huang","year":"2023","journal-title":"Nature Nanotechnology"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"989","DOI":"10.1126\/science.287.5455.989","type":"journal-article","article-title":"Conservation and novelty in the evolution of cell adhesion and extracellular matrix genes","volume":"287","author":"Hutter","year":"2000","journal-title":"Science"},{"key":"bib29","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-4-35","type":"journal-article","article-title":"The eggshell is required for meiotic fidelity, polar-body extrusion and polarization of the C. elegans embryo","volume":"4","author":"Johnston","year":"2006","journal-title":"BMC Biology"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"1932","DOI":"10.1016\/j.cub.2010.09.059","type":"journal-article","article-title":"Eggshell chitin and chitin-interacting proteins prevent polyspermy in C. elegans","volume":"20","author":"Johnston","year":"2010","journal-title":"Current Biology"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1002\/dvg.20823","type":"journal-article","article-title":"The eggshell in the C. elegans oocyte-to-embryo transition","volume":"50","author":"Johnston","year":"2012","journal-title":"Genesis"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"2655","DOI":"10.1534\/g3.118.200278","type":"journal-article","article-title":"Auxin-mediated sterility induction system for longevity and mating studies in Caenorhabditis elegans","volume":"8","author":"Kasimatis","year":"2018","journal-title":"G3: Genes, Genomes, Genetics"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"531","DOI":"10.1098\/rspb.1979.0083","type":"journal-article","article-title":"The evolution of ageing and longevity","volume":"205","author":"Kirkwood","year":"1979","journal-title":"Proceedings of the Royal Society of London. Series B, Biological Sciences"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"3635","DOI":"10.1523\/JNEUROSCI.2150-20.2021","type":"journal-article","article-title":"The sex-specific VC neurons are mechanically activated motor neurons that facilitate serotonin-induced egg laying in C. elegans","volume":"41","author":"Kopchock","year":"2021","journal-title":"The Journal of Neuroscience"},{"key":"bib35","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1093\/ilar.50.1.51","type":"journal-article","article-title":"The biopsychology of maternal behavior in nonhuman mammals","volume":"50","author":"Kristal","year":"2009","journal-title":"ILAR Journal"},{"key":"bib36","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002678","type":"journal-article","article-title":"SPE-44 implements sperm cell fate","volume":"8","author":"Kulkarni","year":"2012","journal-title":"PLOS Genetics"},{"key":"bib37","doi-asserted-by":"publisher","DOI":"10.12703\/P6-7","type":"journal-article","article-title":"Proteostasis and longevity: when does aging really begin?","volume":"6","author":"Labbadia","year":"2014","journal-title":"F1000prime Reports"},{"key":"bib38","doi-asserted-by":"publisher","first-page":"639","DOI":"10.1016\/j.molcel.2015.06.027","type":"journal-article","article-title":"Repression of the heat shock response is a programmed event at the onset of reproduction","volume":"59","author":"Labbadia","year":"2015","journal-title":"Molecular Cell"},{"key":"bib39","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2022.105753","type":"journal-article","article-title":"Neuron-astrocyte transmitophagy is altered in Alzheimer\u2019s disease","volume":"170","author":"Lampinen","year":"2022","journal-title":"Neurobiology of Disease"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"2990","DOI":"10.1038\/emboj.2011.195","type":"journal-article","article-title":"EGF signalling activates the ubiquitin proteasome system to modulate C. elegans lifespan","volume":"30","author":"Liu","year":"2011","journal-title":"The EMBO Journal"},{"key":"bib41","doi-asserted-by":"publisher","first-page":"1605","DOI":"10.1016\/j.cub.2005.07.068","type":"journal-article","article-title":"The BTB protein MEL-26 promotes cytokinesis in C. elegans by a CUL-3-independent mechanism","volume":"15","author":"Luke-Glaser","year":"2005","journal-title":"Current Biology"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1007\/s11906-022-01221-5","type":"journal-article","article-title":"Effects of hypertension on alzheimer\u2019s disease and related disorders","volume":"24","author":"Malone","year":"2022","journal-title":"Current Hypertension Reports"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1006\/dbio.1998.9109","type":"journal-article","article-title":"On the control of oocyte meiotic maturation and ovulation in Caenorhabditis elegans","volume":"205","author":"McCarter","year":"1999","journal-title":"Developmental Biology"},{"key":"bib44","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-7-41","type":"journal-article","article-title":"A role for sperm in regulation of egg-laying in the nematode C. elegans","volume":"7","author":"McGovern","year":"2007","journal-title":"BMC Developmental Biology"},{"key":"bib45","doi-asserted-by":"publisher","first-page":"2330","DOI":"10.1016\/j.cub.2023.05.008","type":"journal-article","article-title":"Muscle-directed mechanosensory feedback activates egg-laying circuit activity and behavior in Caenorhabditis elegans","volume":"33","author":"Medrano","year":"2023","journal-title":"Current Biology"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1042\/BJ20031213","type":"journal-article","article-title":"Latrophilin is required for toxicity of black widow spider venom in Caenorhabditis elegans","volume":"378","author":"Mee","year":"2004","journal-title":"The Biochemical Journal"},{"key":"bib47","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1038\/nature21362","type":"journal-article","article-title":"C. elegans neurons jettison protein aggregates and mitochondria under neurotoxic stress","volume":"542","author":"Melentijevic","year":"2017","journal-title":"Nature"},{"key":"bib48","doi-asserted-by":"publisher","first-page":"2144","DOI":"10.1126\/science.1057586","type":"journal-article","article-title":"A sperm cytoskeletal protein that signals oocyte meiotic maturation and ovulation","volume":"291","author":"Miller","year":"2001","journal-title":"Science"},{"key":"bib49","doi-asserted-by":"publisher","first-page":"558","DOI":"10.1016\/j.stem.2020.01.018","type":"journal-article","article-title":"Vimentin coordinates protein turnover at the aggresome during neural stem cell quiescence exit","volume":"26","author":"Morrow","year":"2020","journal-title":"Cell Stem Cell"},{"key":"bib50","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1016\/j.cell.2020.08.031","type":"journal-article","article-title":"A network of macrophages supports mitochondrial homeostasis in the heart","volume":"183","author":"Nicol\u00e1s-\u00c1vila","year":"2020","journal-title":"Cell"},{"key":"bib51","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2020.100286","type":"journal-article","article-title":"Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting","volume":"2","author":"Nicol\u00e1s-\u00c1vila","year":"2021","journal-title":"STAR Protocols"},{"key":"bib52","doi-asserted-by":"publisher","first-page":"418","DOI":"10.1161\/CIRCRESAHA.121.319812","type":"journal-article","article-title":"Macrophages, metabolism and heterophagy in the heart","volume":"130","author":"Nicol\u00e1s-\u00c1vila","year":"2022","journal-title":"Circulation Research"},{"key":"bib53","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1038\/nmeth.1401","type":"journal-article","article-title":"An auxin-based degron system for the rapid depletion of proteins in nonplant cells","volume":"6","author":"Nishimura","year":"2009","journal-title":"Nature Methods"},{"key":"bib54","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1083\/jcb.201206008","type":"journal-article","article-title":"Hierarchical assembly of the eggshell and permeability barrier in C. elegans","volume":"198","author":"Olson","year":"2012","journal-title":"The Journal of Cell Biology"},{"key":"bib55","doi-asserted-by":"publisher","first-page":"758","DOI":"10.1038\/nrm.2017.87","type":"journal-article","article-title":"Mechanobiology of YAP and TAZ in physiology and disease","volume":"18","author":"Panciera","year":"2017","journal-title":"Nature Reviews. Molecular Cell Biology"},{"key":"bib56","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1038\/s41582-020-0333-7","type":"journal-article","article-title":"Protein transmission in neurodegenerative disease","volume":"16","author":"Peng","year":"2020","journal-title":"Nature Reviews. Neurology"},{"key":"bib57","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.ebiom.2018.01.021","type":"journal-article","article-title":"Traumatic brain injury and alzheimer\u2019s disease: The cerebrovascular link","volume":"28","author":"Ramos-Cejudo","year":"2018","journal-title":"EBioMedicine"},{"key":"bib58","doi-asserted-by":"publisher","first-page":"2838","DOI":"10.1101\/gad.13.21.2838","type":"journal-article","article-title":"The coronin-like protein POD-1 is required for anterior-posterior axis formation and cellular architecture in the nematode Caenorhabditis elegans","volume":"13","author":"Rappleye","year":"1999","journal-title":"Genes & Development"},{"key":"bib59","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-3-8","type":"journal-article","article-title":"Involvement of fatty acid pathways and cortical interaction of the pronuclear complex in Caenorhabditis elegans embryonic polarity","volume":"3","author":"Rappleye","year":"2003","journal-title":"BMC Developmental Biology"},{"key":"bib60","doi-asserted-by":"publisher","first-page":"6283","DOI":"10.1523\/JNEUROSCI.3658-17.2018","type":"journal-article","article-title":"Homeostatic feedback modulates the development of two-state patterned activity in a model serotonin motor circuit in Caenorhabditis elegans","volume":"38","author":"Ravi","year":"2018","journal-title":"The Journal of Neuroscience"},{"key":"bib61","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyab080","type":"journal-article","article-title":"Presynaptic G\u03b1o (GOA-1) signals to depress command neuron excitability and allow stretch-dependent modulation of egg laying in Caenorhabditis elegans","volume":"218","author":"Ravi","year":"2021","journal-title":"Genetics"},{"key":"bib62","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1895\/wormbook.1.30.2","type":"journal-article","article-title":"Polarity establishment, asymmetric division and segregation of fate determinants in early C. elegans embryos","volume":"1","author":"Rose","year":"2014","journal-title":"WormBook"},{"key":"bib63","doi-asserted-by":"publisher","first-page":"678","DOI":"10.1101\/gad.335422.119","type":"journal-article","article-title":"Embryo integrity regulates maternal proteostasis and stress resilience","volume":"34","author":"Sala","year":"2020","journal-title":"Genes & Development"},{"key":"bib64","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1016\/j.tcb.2021.09.009","type":"journal-article","article-title":"Protecting the future: balancing proteostasis for reproduction","volume":"32","author":"Sala","year":"2022","journal-title":"Trends in Cell Biology"},{"key":"bib65","doi-asserted-by":"publisher","first-page":"2121","DOI":"10.1007\/s11064-018-2636-2","type":"journal-article","article-title":"Deciphering the role of EGL-3 for neuropeptides processing in Caenorhabditis elegans using high-resolution quadrupole-orbitrap mass spectrometry","volume":"43","author":"Salem","year":"2018","journal-title":"Neurochemical Research"},{"key":"bib66","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1016\/0012-1606(80)90368-1","type":"journal-article","article-title":"Cell lineages and developmental defects of temperature-sensitive embryonic arrest mutants in Caenorhabditis elegans","volume":"76","author":"Schierenberg","year":"1980","journal-title":"Developmental Biology"},{"key":"bib67","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1146\/annurev.genet.37.110801.142443","type":"journal-article","article-title":"Cell polarity and the cytoskeleton in the Caenorhabditis elegans zygote","volume":"37","author":"Schneider","year":"2003","journal-title":"Annual Review of Genetics"},{"key":"bib68","doi-asserted-by":"publisher","first-page":"572","DOI":"10.1002\/dvg.22749","type":"journal-article","article-title":"Timing and mechanism of the initial cue establishing handed left\u2013right asymmetry in Caenorhabditis elegans embryos","volume":"52","author":"Schonegg","year":"2014","journal-title":"Genesis"},{"key":"bib69","doi-asserted-by":"publisher","first-page":"2066","DOI":"10.1016\/s0960-9822(02)01355-6","type":"journal-article","article-title":"A Formin Homology protein and A profilin are required for cytokinesis and Arp2\/3-independent assembly of cortical microfilaments in C. elegans","volume":"12","author":"Severson","year":"2002","journal-title":"Current Biology"},{"key":"bib70","doi-asserted-by":"publisher","first-page":"462","DOI":"10.1038\/nature03353","type":"journal-article","article-title":"Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans","volume":"434","author":"S\u00f6nnichsen","year":"2005","journal-title":"Nature"},{"key":"bib71","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1895\/wormbook.1.179.1","type":"journal-article","article-title":"The C. elegans eggshell","volume":"1","author":"Stein","year":"2018","journal-title":"WormBook"},{"key":"bib72","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1895\/wormbook.1.6.1","type":"journal-article","article-title":"Vulval development","volume":"1","author":"Sternberg","year":"2005","journal-title":"WormBook"},{"key":"bib73","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/S0092-8674(00)80603-0","type":"journal-article","article-title":"MIG-13 positions migrating cells along the anteroposterior body axis of C. elegans","volume":"98","author":"Sym","year":"1999","journal-title":"Cell"},{"key":"bib74","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1016\/j.cub.2014.11.033","type":"journal-article","article-title":"Transient membrane Localization of SPV-1 drives cyclical actomyosin contractions in the C. elegans spermatheca","volume":"25","author":"Tan","year":"2015","journal-title":"Current Biology"},{"key":"bib75","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2023.108158","type":"journal-article","article-title":"The role of biomechanical stress in extracellular vesicle formation, composition and activity","volume":"66","author":"Thompson","year":"2023","journal-title":"Biotechnology Advances"},{"key":"bib76","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213x-1-2","type":"journal-article","article-title":"High copy arrays containing a sequence upstream of mec-3 alter cell migration and axonal morphology in C. elegans","volume":"1","author":"Toms","year":"2001","journal-title":"BMC Developmental Biology"},{"key":"bib77","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1093\/genetics\/104.4.619","type":"journal-article","article-title":"Egg-laying defective mutants of the nematode Caenorhabditis elegans","volume":"104","author":"Trent","year":"1983","journal-title":"Genetics"},{"key":"bib78","doi-asserted-by":"publisher","DOI":"10.15252\/embr.202052071","type":"journal-article","article-title":"Muscle-derived exophers promote reproductive fitness","volume":"22","author":"Turek","year":"2021","journal-title":"EMBO Reports"},{"key":"bib79","doi-asserted-by":"publisher","first-page":"1181","DOI":"10.1093\/genetics\/154.3.1181","type":"journal-article","article-title":"Effect of a neuropeptide gene on behavioral states in Caenorhabditis elegans egg-laying","volume":"154","author":"Waggoner","year":"2000","journal-title":"Genetics"},{"key":"bib80","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/j.mod.2005.11.003","type":"journal-article","article-title":"Transcriptional upregulation of the C. elegans Hox gene lin-39 during vulval cell fate specification","volume":"123","author":"Wagmaister","year":"2006","journal-title":"Mechanisms of Development"},{"key":"bib81","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.82227","type":"journal-article","article-title":"Large vesicle extrusions from C. elegans neurons are consumed and stimulated by glial-like phagocytosis activity of the neighboring cell","volume":"12","author":"Wang","year":"2023","journal-title":"eLife"},{"key":"bib82","doi-asserted-by":"publisher","first-page":"1374","DOI":"10.1016\/j.cub.2004.07.056","type":"journal-article","article-title":"Latrotoxin receptor signaling engages the UNC-13-dependent vesicle-priming pathway in C. elegans","volume":"14","author":"Willson","year":"2004","journal-title":"Current Biology"},{"key":"bib83","doi-asserted-by":"publisher","DOI":"10.1101\/2022.12.12.520171","type":"preprint","article-title":"Inhibition of early-acting autophagy genes in C. elegans neurons improves protein homeostasis, promotes exopher production, and extends lifespan via the ATG-16.2 WD40 domain","volume-title":"bioRxiv","author":"Yang","year":"2022"},{"key":"bib84","doi-asserted-by":"publisher","first-page":"1385","DOI":"10.1261\/rna.031237.111","type":"journal-article","article-title":"The sperm-oocyte switch in the C. elegans hermaphrodite is controlled through steady-state levels of the fem-3 mRNA","volume":"18","author":"Zanetti","year":"2012","journal-title":"RNA"},{"key":"bib85","doi-asserted-by":"publisher","first-page":"4374","DOI":"10.1242\/dev.129635","type":"journal-article","article-title":"The auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans","volume":"142","author":"Zhang","year":"2015","journal-title":"Development"}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/95443\/elife-95443-v1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/95443\/elife-95443-v1.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/95443","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:13:02Z","timestamp":1787566382000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/95443"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,10]]},"references-count":85,"alternative-id":["10.7554\/eLife.95443"],"URL":"https:\/\/doi.org\/10.7554\/elife.95443","archive":["CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2023.11.13.565361","asserted-by":"subject"}],"is-same-as":[{"id-type":"doi","id":"10.7554\/eLife.95443.3","asserted-by":"subject"}],"is-version-of":[{"id-type":"doi","id":"10.7554\/eLife.95443.1","asserted-by":"subject"},{"id-type":"doi","id":"10.7554\/eLife.95443.2","asserted-by":"subject"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,10]]},"assertion":[{"value":"single anonymised","name":"peer_review_transparency","label":"Peer review transparency","group":{"name":"peer_review_taxonomy"}},{"value":"other reviewer(s), editor","name":"peer_review_interaction","label":"Peer review interaction","group":{"name":"peer_review_taxonomy"}},{"value":"review summaries, review reports, author\/editor communication, reviewer identities reviewer opt in, editor identities","name":"peer_review_published","label":"Peer review published","group":{"name":"peer_review_taxonomy"}},{"value":"open (sign in with ORCID iD required)","name":"post_publication_commenting","label":"Post publication commenting","group":{"name":"post_publication_commenting"}}],"article-number":"RP95443"}}