{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T04:42:47Z","timestamp":1790656967886,"version":"4.1.0"},"reference-count":59,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T00:00:00Z","timestamp":1750982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"},{"start":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T00:00:00Z","timestamp":1750982400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"},{"start":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T00:00:00Z","timestamp":1750982400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000015","name":"U.S. Department of Energy","doi-asserted-by":"publisher","award":["DE-AC36-08GO28308"],"award-info":[{"award-number":["DE-AC36-08GO28308"]}],"id":[{"id":"10.13039\/100000015","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006228","name":"Oak Ridge National Laboratory","doi-asserted-by":"publisher","award":["ERKP886"],"award-info":[{"award-number":["ERKP886"]}],"id":[{"id":"10.13039\/100006228","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    <jats:italic>Clostridium thermocellum<\/jats:italic>\n                    , a cellulolytic thermophilic anaerobe, is considered by many to be a prime candidate for the realization of consolidated bioprocessing (CBP) and is known as an industry standard for biofuel production.\n                    <jats:italic>C. thermocellum<\/jats:italic>\n                    is among the best biomass degraders identified to date in nature and produces ethanol as one of its main products. Many studies have helped increase ethanol titers in this microbe; however, ethanol production using\n                    <jats:italic>C. thermocellum<\/jats:italic>\n                    is still not economically viable. Therefore, a better understanding of its ethanol synthesis pathway is required. The main pathway for ethanol production in\n                    <jats:italic>C. thermocellum<\/jats:italic>\n                    involves the bifunctional aldehyde-alcohol dehydrogenase (AdhE). To better understand the function of the\n                    <jats:italic>C. thermocellum<\/jats:italic>\n                    AdhE, we used cryo-electron microscopy (cryo-EM) to obtain a 3.28 \u00c5 structure of the AdhE complex. This high-resolution structure, in combination with molecular dynamics simulations, provides insight into the substrate channeling of the toxic intermediate acetaldehyde, indicates the potential role of\n                    <jats:italic>C. thermocellum<\/jats:italic>\n                    AdhE to regulate activity and cofactor pools, and establishes a basis for future engineering studies. The containment strategy found in this enzyme offers a template that could be replicated in other systems where toxic intermediates need to be sequestered to increase the production of valuable biochemicals.\n                  <\/jats:p>","DOI":"10.7554\/elife.96966","type":"journal-article","created":{"date-parts":[[2024,5,24]],"date-time":"2024-05-24T12:25:21Z","timestamp":1716553521000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.96966","source":"Crossref","is-referenced-by-count":1,"title":["Structural characterization and dynamics of AdhE ultrastructures from Clostridium thermocellum show a containment strategy for toxic intermediates"],"prefix":"10.7554","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2480-513X","authenticated-orcid":true,"given":"Samantha J","family":"Ziegler","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036266993","id-type":"ROR","asserted-by":"publisher"}],"name":"Biosciences Center, National Renewable Energy 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