{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:39:38Z","timestamp":1787567978164,"version":"build-2736575974"},"reference-count":99,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T00:00:00Z","timestamp":1733788800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T00:00:00Z","timestamp":1733788800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T00:00:00Z","timestamp":1733788800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AI166465"],"award-info":[{"award-number":["R21AI166465"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R03AI153902"],"award-info":[{"award-number":["R03AI153902"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100002570","name":"American Association of Immunologists","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100002570","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["S10OD026843"],"award-info":[{"award-number":["S10OD026843"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    During thymic development, most \u03b3\u03b4 T cells acquire innate-like characteristics that are critical for their function in tumor surveillance, infectious disease, and tissue repair. The mechanisms, however, that regulate \u03b3\u03b4 T cell developmental programming remain unclear. Recently, we demonstrated that the SLAM\/SAP signaling pathway regulates the development and function of multiple innate-like \u03b3\u03b4 T cell subsets. Here, we used a single-cell proteogenomics approach to identify SAP-dependent developmental checkpoints and to define the SAP-dependent \u03b3\u03b4 TCR repertoire in mice. SAP deficiency resulted in both a significant loss of an immature\n                    <jats:italic>Gzma<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    <jats:italic>\n                      Blk\n                      <jats:sup>+<\/jats:sup>\n                      Etv5\n                      <jats:sup>+<\/jats:sup>\n                      Tox2\n                      <jats:sup>+<\/jats:sup>\n                    <\/jats:italic>\n                    \u03b3\u03b4T17 precursor population and a significant increase in\n                    <jats:italic>\n                      Cd4\n                      <jats:sup>+<\/jats:sup>\n                      Cd8\n                      <jats:sup>+<\/jats:sup>\n                      Rorc\n                      <jats:sup>+<\/jats:sup>\n                      Ptcra\n                      <jats:sup>+<\/jats:sup>\n                      Rag1\n                      <jats:sup>+<\/jats:sup>\n                    <\/jats:italic>\n                    thymic \u03b3\u03b4 T cells. SAP-dependent diversion of embryonic day 17 thymic \u03b3\u03b4 T cell clonotypes into the \u03b1\u03b2 T cell developmental pathway was associated with a decreased frequency of mature clonotypes in neonatal thymus, and an altered \u03b3\u03b4 TCR repertoire in the periphery. Finally, we identify TRGV4\/TRAV13-4(DV7)-expressing T cells as a novel, SAP-dependent V\u03b34 \u03b3\u03b4T1 subset. Together, the data support a model in which SAP-dependent \u03b3\u03b4\/\u03b1\u03b2 T cell lineage commitment regulates \u03b3\u03b4 T cell developmental programming and shapes the \u03b3\u03b4 TCR repertoire.\n                  <\/jats:p>","DOI":"10.7554\/elife.97229","type":"journal-article","created":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T12:25:10Z","timestamp":1715343910000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.97229","source":"Crossref","is-referenced-by-count":10,"title":["SLAM\/SAP signaling regulates discrete \u03b3\u03b4 T cell developmental checkpoints and shapes the innate-like \u03b3\u03b4 TCR repertoire"],"prefix":"10.7554","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2139-6927","authenticated-orcid":true,"given":"Somen K","family":"Mistri","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0155zta11","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Surgery, Larner College of Medicine, 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