{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:06:57Z","timestamp":1790122017850,"version":"4.0.1"},"reference-count":95,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T00:00:00Z","timestamp":1727308800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T00:00:00Z","timestamp":1727308800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T00:00:00Z","timestamp":1727308800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2020R1A5A1018081"],"award-info":[{"award-number":["NRF-2020R1A5A1018081"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2022R1A2C3007621"],"award-info":[{"award-number":["NRF-2022R1A2C3007621"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Fibro-adipogenic progenitors (FAPs) are muscle-resident mesenchymal progenitors that can contribute to muscle tissue homeostasis and regeneration, as well as postnatal maturation and lifelong maintenance of the neuromuscular system. Recently, traumatic injury to the peripheral nerve was shown to activate FAPs, suggesting that FAPs can respond to nerve injury. However, questions of how FAPs can sense the anatomically distant peripheral nerve injury and whether FAPs can directly contribute to nerve regeneration remained unanswered. Here, utilizing single-cell transcriptomics and mouse models, we discovered that a subset of FAPs expressing GDNF receptors\n                    <jats:italic>Ret<\/jats:italic>\n                    and\n                    <jats:italic>Gfra1<\/jats:italic>\n                    can respond to peripheral nerve injury by sensing GDNF secreted by Schwann cells. Upon GDNF sensing, this subset becomes activated and expresses\n                    <jats:italic>Bdnf<\/jats:italic>\n                    . FAP-specific inactivation of\n                    <jats:italic>Bdnf<\/jats:italic>\n                    (\n                    <jats:italic>\n                      Prrx1\n                      <jats:sup>Cre<\/jats:sup>\n                      ; Bdnf\n                      <jats:sup>fl\/fl<\/jats:sup>\n                    <\/jats:italic>\n                    ) resulted in delayed nerve regeneration owing to defective remyelination, indicating that GDNF-sensing FAPs play an important role in the remyelination process during peripheral nerve regeneration. In aged mice, significantly reduced\n                    <jats:italic>Bdnf<\/jats:italic>\n                    expression in FAPs was observed upon nerve injury, suggesting the clinical relevance of FAP-derived BDNF in the age-related delays in nerve regeneration. Collectively, our study revealed the previously unidentified role of FAPs in peripheral nerve regeneration, and the molecular mechanism behind FAPs\u2019 response to peripheral nerve injury.\n                  <\/jats:p>","DOI":"10.7554\/elife.97662","type":"journal-article","created":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T07:33:15Z","timestamp":1717486395000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.97662","source":"Crossref","is-referenced-by-count":6,"title":["Muscle-resident mesenchymal progenitors sense and repair peripheral nerve injury via the GDNF-BDNF axis"],"prefix":"10.7554","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9852-5344","authenticated-orcid":true,"given":"Kyusang","family":"Yoo","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04h9pn542","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Biological Sciences, Seoul National University","place":["Seoul, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Young-Woo","family":"Jo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04h9pn542","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Biological Sciences, Seoul National University","place":["Seoul, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4381-7553","authenticated-orcid":true,"given":"Takwon","family":"Yoo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04h9pn542","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Biological Sciences, Seoul National University","place":["Seoul, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"software","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0871-3414","authenticated-orcid":true,"given":"Sang-Hyeon","family":"Hann","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04h9pn542","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Biological Sciences, Seoul National University","place":["Seoul, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"resources","vocabulary":"credit"}]},{"given":"Inkuk","family":"Park","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04h9pn542","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Biological Sciences, Seoul National University","place":["Seoul, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"resources","vocabulary":"credit"}]},{"given":"Yea-Eun","family":"Kim","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04h9pn542","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Biological Sciences, Seoul National University","place":["Seoul, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"resources","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Ye Lynne","family":"Kim","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04h9pn542","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Biological Sciences, Seoul National University","place":["Seoul, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Joonwoo","family":"Rhee","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04h9pn542","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Biological Sciences, Seoul National University","place":["Seoul, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"resources","vocabulary":"credit"}]},{"given":"In-Wook","family":"Song","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04h9pn542","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Biological Sciences, Seoul National University","place":["Seoul, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"resources","vocabulary":"credit"}]},{"given":"Ji-Hoon","family":"Kim","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04h9pn542","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Biological Sciences, Seoul National University","place":["Seoul, Republic of Korea"]},{"id":[{"id":"https:\/\/ror.org\/05kzfa883","id-type":"ROR","asserted-by":"publisher"}],"name":"Molecular Recognition Research Center, Korea Institute of Science and Technology","place":["Seoul, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"resources","vocabulary":"credit"}]},{"given":"Daehyun","family":"Baek","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04h9pn542","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Biological Sciences, Seoul National University","place":["Seoul, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"validation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7335-3729","authenticated-orcid":true,"given":"Young-Yun","family":"Kong","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04h9pn542","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Biological Sciences, Seoul National University","place":["Seoul, Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"corresponding-author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"4374","published-online":{"date-parts":[[2024,9,26]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1038\/nrn812","type":"journal-article","article-title":"The GDNF family: signalling, biological functions and therapeutic value","volume":"3","author":"Airaksinen","year":"2002","journal-title":"Nature Reviews. Neuroscience"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"633","DOI":"10.1016\/j.neuron.2012.06.021","type":"journal-article","article-title":"c-Jun reprograms Schwann cells of injured nerves to generate a repair cell essential for regeneration","volume":"75","author":"Arthur-Farraj","year":"2012","journal-title":"Neuron"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.stem.2014.06.009","type":"journal-article","article-title":"Immune modulation of stem cells and regeneration","volume":"15","author":"Aurora","year":"2014","journal-title":"Cell Stem Cell"},{"key":"bib4","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.adg1485","type":"journal-article","article-title":"Regeneration of neuromuscular synapses after acute and chronic denervation by inhibiting the gerozyme 15-prostaglandin dehydrogenase","volume":"15","author":"Bakooshli","year":"2023","journal-title":"Science Translational Medicine"},{"key":"bib5","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2014.00254","type":"journal-article","article-title":"BDNF signaling and survival of striatal neurons","volume":"8","author":"Baydyuk","year":"2014","journal-title":"Frontiers in Cellular Neuroscience"},{"key":"bib6","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12833","type":"journal-article","article-title":"Inflammaging impairs peripheral nerve maintenance and regeneration","volume":"17","author":"B\u00fcttner","year":"2018","journal-title":"Aging Cell"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1016\/j.stem.2018.10.024","type":"journal-article","article-title":"Mesenchymal precursor cells in adult nerves contribute to mammalian tissue repair and regeneration","volume":"24","author":"Carr","year":"2019","journal-title":"Cell Stem Cell"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"14661","DOI":"10.1073\/pnas.251543398","type":"journal-article","article-title":"Neurotrophins are key mediators of the myelination program in the peripheral nervous system","volume":"98","author":"Chan","year":"2001","journal-title":"PNAS"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/j.nic.2013.03.023","type":"journal-article","article-title":"Peripheral neuropathy: clinical and electrophysiological considerations","volume":"24","author":"Chung","year":"2014","journal-title":"Neuroimaging Clinics of North America"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1007\/s00441-020-03287-6","type":"journal-article","article-title":"GDNF synthesis, signaling, and retrograde transport in motor neurons","volume":"382","author":"Cintr\u00f3n-Col\u00f3n","year":"2020","journal-title":"Cell and Tissue Research"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"647","DOI":"10.1007\/s00441-015-2343-0","type":"journal-article","article-title":"Connective tissue cells expressing fibro\/adipogenic progenitor markers increase under chronic damage: relevance in fibroblast-myofibroblast differentiation and skeletal muscle fibrosis","volume":"364","author":"Contreras","year":"2016","journal-title":"Cell and Tissue Research"},{"key":"bib12","doi-asserted-by":"publisher","DOI":"10.1186\/s13395-021-00265-6","type":"journal-article","article-title":"Origins, potency, and heterogeneity of skeletal muscle fibro-adipogenic progenitors-time for new definitions","volume":"11","author":"Contreras","year":"2021","journal-title":"Skeletal Muscle"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"3583","DOI":"10.1016\/j.celrep.2020.02.067","type":"journal-article","article-title":"Single-cell analysis of the muscle stem cell hierarchy identifies heterotypic communication signals involved in skeletal muscle regeneration","volume":"30","author":"De Micheli","year":"2020","journal-title":"Cell Reports"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1002\/gene.10154","type":"journal-article","article-title":"Inducible site-specific recombination in myelinating cells","volume":"35","author":"Doerflinger","year":"2003","journal-title":"Genesis"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"1464","DOI":"10.1523\/JNEUROSCI.21-05-01464.2001","type":"journal-article","article-title":"c-Src is required for glial cell line-derived neurotrophic factor (GDNF) family ligand-mediated neuronal survival via a phosphatidylinositol-3 kinase (PI-3K)-dependent pathway","volume":"21","author":"Encinas","year":"2001","journal-title":"The Journal of Neuroscience"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"16217","DOI":"10.1073\/pnas.1303646110","type":"journal-article","article-title":"Small-molecule trkB agonists promote axon regeneration in cut peripheral nerves","volume":"110","author":"English","year":"2013","journal-title":"PNAS"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/j.neo.2017.10.010","type":"journal-article","article-title":"The GDNF Family: a role in cancer?","volume":"20","author":"Fielder","year":"2018","journal-title":"Neoplasia"},{"key":"bib18","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1002\/glia.23705","type":"journal-article","article-title":"Prostaglandin D2 synthase modulates macrophage activity and accumulation in injured peripheral nerves","volume":"68","author":"Forese","year":"2020","journal-title":"Glia"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"1618","DOI":"10.1126\/science.7907431","type":"journal-article","article-title":"Requirement for BDNF in activity-dependent survival of cortical neurons","volume":"263","author":"Ghosh","year":"1994","journal-title":"Science"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"8953","DOI":"10.1523\/JNEUROSCI.0219-10.2010","type":"journal-article","article-title":"Elevated phosphatidylinositol 3,4,5-trisphosphate in glia triggers cell-autonomous membrane wrapping and myelination","volume":"30","author":"Goebbels","year":"2010","journal-title":"The Journal of Neuroscience"},{"key":"bib21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0177649","type":"journal-article","article-title":"ALS skeletal muscle shows enhanced TGF-\u03b2 signaling, fibrosis and induction of fibro\/adipogenic progenitor markers","volume":"12","author":"Gonzalez","year":"2017","journal-title":"PLOS ONE"},{"key":"bib22","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/698256","type":"journal-article","article-title":"Peripheral nerve reconstruction after injury: a review of clinical and experimental therapies","volume":"2014","author":"Grinsell","year":"2014","journal-title":"BioMed Research International"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"857","DOI":"10.1097\/00001756-199603220-00004","type":"journal-article","article-title":"GDNF mRNA in Schwann cells and DRG satellite cells after chronic sciatic nerve injury","volume":"7","author":"Hammarberg","year":"1996","journal-title":"Neuroreport"},{"key":"bib24","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx1013","type":"journal-article","article-title":"TRRUST v2","volume":"46","author":"Han","year":"2018","journal-title":"Nucleic Acids Research"},{"key":"bib25","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.92731","type":"journal-article","article-title":"Depletion of SMN protein in mesenchymal progenitors impairs the development of bone and neuromuscular junction in spinal muscular atrophy","volume":"12","author":"Hann","year":"2024","journal-title":"eLife"},{"key":"bib26","doi-asserted-by":"publisher","first-page":"1095","DOI":"10.1016\/s0896-6273(03)00770-0","type":"journal-article","article-title":"Axoplasmic importins enable retrograde injury signaling in lesioned nerve","volume":"40","author":"Hanz","year":"2003","journal-title":"Neuron"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"3573","DOI":"10.1016\/j.cell.2021.04.048","type":"journal-article","article-title":"Integrated analysis of multimodal single-cell data","volume":"184","author":"Hao","year":"2021","journal-title":"Cell"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1016\/j.cell.2013.02.053","type":"journal-article","article-title":"Type 2 innate signals stimulate fibro\/adipogenic progenitors to facilitate muscle regeneration","volume":"153","author":"Heredia","year":"2013","journal-title":"Cell"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1006\/exnr.2001.7826","type":"journal-article","article-title":"A decline in glial cell-line-derived neurotrophic factor expression is associated with impaired regeneration after long-term Schwann cell denervation","volume":"173","author":"H\u00f6ke","year":"2002","journal-title":"Experimental Neurology"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"1113","DOI":"10.1016\/s0092-8674(00)81311-2","type":"journal-article","article-title":"GDNF-induced activation of the ret protein tyrosine kinase is mediated by GDNFR-alpha, a novel receptor for GDNF","volume":"85","author":"Jing","year":"1996","journal-title":"Cell"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1038\/ncb2015","type":"journal-article","article-title":"Muscle injury activates resident fibro\/adipogenic progenitors that facilitate myogenesis","volume":"12","author":"Joe","year":"2010","journal-title":"Nature Cell Biology"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"5599","DOI":"10.1242\/dev.01429","type":"journal-article","article-title":"Neural crest stem cells undergo multilineage differentiation in developing peripheral nerves to generate endoneurial fibroblasts in addition to Schwann cells","volume":"131","author":"Joseph","year":"2004","journal-title":"Development"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.actbio.2017.01.056","type":"journal-article","article-title":"Promoting tissue regeneration by modulating the immune system","volume":"53","author":"Julier","year":"2017","journal-title":"Acta Biomaterialia"},{"key":"bib34","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.60223","type":"journal-article","article-title":"Analysis of the immune response to sciatic nerve injury identifies efferocytosis as a key mechanism of nerve debridement","volume":"9","author":"Kalinski","year":"2020","journal-title":"eLife"},{"key":"bib35","doi-asserted-by":"publisher","first-page":"D587","DOI":"10.1093\/nar\/gkac963","type":"journal-article","article-title":"KEGG for taxonomy-based analysis of pathways and genomes","volume":"51","author":"Kanehisa","year":"2023","journal-title":"Nucleic Acids Research"},{"key":"bib36","doi-asserted-by":"publisher","first-page":"94","DOI":"10.4103\/0976-500X.72351","type":"journal-article","article-title":"Improving bioscience research reporting: The ARRIVE guidelines for reporting animal research","volume":"1","author":"Kilkenny","year":"2010","journal-title":"Journal of Pharmacology & Pharmacotherapeutics"},{"key":"bib37","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.158380","type":"journal-article","article-title":"Bap1\/SMN axis in Dpp4+ skeletal muscle mesenchymal cells regulates the neuromuscular system","volume":"7","author":"Kim","year":"2022","journal-title":"JCI Insight"},{"key":"bib38","doi-asserted-by":"publisher","first-page":"W207","DOI":"10.1093\/nar\/gkad347","type":"journal-article","article-title":"g:Profiler-interoperable web service for functional enrichment analysis and gene identifier mapping (2023 update)","volume":"51","author":"Kolberg","year":"2023","journal-title":"Nucleic Acids Research"},{"key":"bib39","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.9090.1","type":"journal-article","article-title":"AutoAnnotate: A Cytoscape app for summarizing networks with semantic annotations","volume":"5","author":"Kucera","year":"2016","journal-title":"F1000Research"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"494","DOI":"10.1038\/s41586-018-0414-6","type":"journal-article","article-title":"RNA velocity of single cells","volume":"560","author":"La Manno","year":"2018","journal-title":"Nature"},{"key":"bib41","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.110785","type":"journal-article","article-title":"Identification of distinct non-myogenic skeletal-muscle-resident mesenchymal cell populations","volume":"39","author":"Leinroth","year":"2022","journal-title":"Cell Reports"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"786","DOI":"10.1038\/nm.3869","type":"journal-article","article-title":"Nilotinib reduces muscle fibrosis in chronic muscle injury by promoting TNF-mediated apoptosis of fibro\/adipogenic progenitors","volume":"21","author":"Lemos","year":"2015","journal-title":"Nature Medicine"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"1","DOI":"10.20517\/jtgg.2020.51","type":"journal-article","article-title":"Toward uncharted territory of cellular heterogeneity: advances and applications of single-cell RNA-seq","volume":"5","author":"Lieberman","year":"2021","journal-title":"Journal of Translational Genetics and Genomics"},{"key":"bib44","doi-asserted-by":"publisher","first-page":"2102","DOI":"10.1002\/jcsm.13023","type":"journal-article","article-title":"Decoding the transcriptome of denervated muscle at single-nucleus resolution","volume":"13","author":"Lin","year":"2022","journal-title":"Journal of Cachexia, Sarcopenia and Muscle"},{"key":"bib45","doi-asserted-by":"publisher","first-page":"1612","DOI":"10.1038\/nprot.2015.110","type":"journal-article","article-title":"Isolation of skeletal muscle stem cells by fluorescence-activated cell sorting","volume":"10","author":"Liu","year":"2015","journal-title":"Nature Protocols"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1002\/gene.10092","type":"journal-article","article-title":"Expression of Cre Recombinase in the developing mouse limb bud driven by a Prxl enhancer","volume":"33","author":"Logan","year":"2002","journal-title":"Genesis"},{"key":"bib47","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1016\/j.stem.2018.12.014","type":"journal-article","article-title":"Aging disrupts muscle stem cell function by impairing matricellular wisp1 secretion from fibro-adipogenic progenitors","volume":"24","author":"Lukjanenko","year":"2019","journal-title":"Cell Stem Cell"},{"key":"bib48","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1038\/s41556-018-0151-y","type":"journal-article","article-title":"Denervation-activated STAT3-IL-6 signalling in fibro-adipogenic progenitors promotes myofibres atrophy and fibrosis","volume":"20","author":"Madaro","year":"2018","journal-title":"Nature Cell Biology"},{"key":"bib49","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1016\/j.expneurol.2007.05.028","type":"journal-article","article-title":"Reinnervation of the tibialis anterior following sciatic nerve crush injury: a confocal microscopic study in transgenic mice","volume":"207","author":"Magill","year":"2007","journal-title":"Experimental Neurology"},{"key":"bib50","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1016\/j.jss.2023.03.017","type":"journal-article","article-title":"Evaluation of the aging effect on peripheral nerve regeneration: a systematic review","volume":"288","author":"Maita","year":"2023","journal-title":"The Journal of Surgical Research"},{"key":"bib51","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06068-6","type":"journal-article","article-title":"Dynamics of cellular states of fibro-adipogenic progenitors during myogenesis and muscular dystrophy","volume":"9","author":"Malecova","year":"2018","journal-title":"Nature Communications"},{"key":"bib52","doi-asserted-by":"publisher","first-page":"ii23","DOI":"10.1136\/jnnp.2005.069138","type":"journal-article","article-title":"Nerve conduction studies: essentials and pitfalls in practice","volume":"76 Suppl 2","author":"Mallik","year":"2005","journal-title":"Journal of Neurology, Neurosurgery, and Psychiatry"},{"key":"bib53","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0013984","type":"journal-article","article-title":"Enrichment map: A network-based method for gene-set enrichment visualization and interpretation","volume":"5","author":"Merico","year":"2010","journal-title":"PLOS ONE"},{"key":"bib54","doi-asserted-by":"publisher","first-page":"2187","DOI":"10.1016\/S0002-9440(10)63070-2","type":"journal-article","article-title":"Rapid response of identified resident endoneurial macrophages to nerve injury","volume":"159","author":"Mueller","year":"2001","journal-title":"The American Journal of Pathology"},{"key":"bib55","doi-asserted-by":"publisher","first-page":"3625","DOI":"10.1242\/dev.064162","type":"journal-article","article-title":"Satellite cells, connective tissue fibroblasts and their interactions are crucial for muscle regeneration","volume":"138","author":"Murphy","year":"2011","journal-title":"Development"},{"key":"bib56","doi-asserted-by":"publisher","DOI":"10.1101\/2020.12.29.424762","type":"preprint","article-title":"scRNA-Seq-Based Analysis of Skeletal Muscle Response to Denervation Reveals Selective Activation of Muscle-Resident Glial Cells and Fibroblasts","volume-title":"bioRxiv","author":"Nicoletti","year":"2020"},{"key":"bib57","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2023.107114","type":"journal-article","article-title":"Muscle denervation promotes functional interactions between glial and mesenchymal cells through NGFR and NGF","volume":"26","author":"Nicoletti","year":"2023","journal-title":"iScience"},{"key":"bib58","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2020.100993","type":"journal-article","article-title":"Temporal dynamics and heterogeneity of cell populations during skeletal muscle regeneration","volume":"23","author":"Oprescu","year":"2020","journal-title":"iScience"},{"key":"bib59","doi-asserted-by":"publisher","first-page":"661","DOI":"10.1002\/emmm.201202382","type":"journal-article","article-title":"Resolution of inflammation: an integrated view","volume":"5","author":"Ortega-G\u00f3mez","year":"2013","journal-title":"EMBO Molecular Medicine"},{"key":"bib60","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1016\/s0006-8993(98)01314-6","type":"journal-article","article-title":"Neurotrophins promote regeneration of sensory axons in the adult rat spinal cord","volume":"818","author":"Oudega","year":"1999","journal-title":"Brain Research"},{"key":"bib61","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1016\/j.neuron.2014.06.016","type":"journal-article","article-title":"Diminished Schwann cell repair responses underlie age-associated impaired axonal regeneration","volume":"83","author":"Painter","year":"2014","journal-title":"Neuron"},{"key":"bib62","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/j.cell.2010.08.039","type":"journal-article","article-title":"EphB signaling directs peripheral nerve regeneration through Sox2-dependent Schwann cell sorting","volume":"143","author":"Parrinello","year":"2010","journal-title":"Cell"},{"key":"bib63","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.143469","type":"journal-article","article-title":"Activation of skeletal muscle-resident glial cells upon nerve injury","volume":"6","author":"Proietti","year":"2021","journal-title":"JCI Insight"},{"key":"bib64","doi-asserted-by":"publisher","first-page":"482","DOI":"10.1038\/s41596-018-0103-9","type":"journal-article","article-title":"Pathway enrichment analysis and visualization of omics data using g:Profiler, GSEA, Cytoscape and EnrichmentMap","volume":"14","author":"Reimand","year":"2019","journal-title":"Nature Protocols"},{"key":"bib65","doi-asserted-by":"publisher","first-page":"1137","DOI":"10.1084\/jem.20122344","type":"journal-article","article-title":"Depletion of stromal cells expressing fibroblast activation protein-\u03b1 from skeletal muscle and bone marrow results in cachexia and anemia","volume":"210","author":"Roberts","year":"2013","journal-title":"The Journal of Experimental Medicine"},{"key":"bib66","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-14734-x","type":"journal-article","article-title":"Exercise enhances skeletal muscle regeneration by promoting senescence in fibro-adipogenic progenitors","volume":"11","author":"Saito","year":"2020","journal-title":"Nature Communications"},{"key":"bib67","doi-asserted-by":"publisher","first-page":"3855","DOI":"10.1242\/jcs.00786","type":"journal-article","article-title":"Novel functions and signalling pathways for GDNF","volume":"116","author":"Sariola","year":"2003","journal-title":"Journal of Cell Science"},{"key":"bib68","doi-asserted-by":"publisher","first-page":"668","DOI":"10.1038\/nrneurol.2013.227","type":"journal-article","article-title":"Advances in peripheral nerve regeneration","volume":"9","author":"Scheib","year":"2013","journal-title":"Nature Reviews. Neurology"},{"key":"bib69","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.neurobiolaging.2016.05.004","type":"journal-article","article-title":"An attenuated immune response by Schwann cells and macrophages inhibits nerve regeneration in aged rats","volume":"45","author":"Scheib","year":"2016","journal-title":"Neurobiology of Aging"},{"key":"bib70","doi-asserted-by":"publisher","first-page":"797","DOI":"10.1016\/j.stem.2019.11.004","type":"journal-article","article-title":"Hic1 defines quiescent mesenchymal progenitor subpopulations with distinct functions and fates in skeletal muscle regeneration","volume":"25","author":"Scott","year":"2019","journal-title":"Cell Stem Cell"},{"key":"bib71","doi-asserted-by":"publisher","first-page":"2498","DOI":"10.1101\/gr.1239303","type":"journal-article","article-title":"Cytoscape: a software environment for integrated models of biomolecular interaction networks","volume":"13","author":"Shannon","year":"2003","journal-title":"Genome Research"},{"key":"bib72","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044592","type":"journal-article","article-title":"Brain-derived neurotrophic factor from bone marrow-derived cells promotes post-injury repair of peripheral nerve","volume":"7","author":"Takemura","year":"2012","journal-title":"PLOS ONE"},{"key":"bib73","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.673404","type":"journal-article","article-title":"Evolving roles of muscle-resident fibro-adipogenic progenitors in health, regeneration, neuromuscular disorders, and aging","volume":"12","author":"Theret","year":"2021","journal-title":"Frontiers in Physiology"},{"key":"bib74","doi-asserted-by":"publisher","DOI":"10.1523\/ENEURO.0066-20.2020","type":"journal-article","article-title":"Peripheral nerve single-cell analysis identifies mesenchymal ligands that promote axonal growth","volume":"7","author":"Toma","year":"2020","journal-title":"eNeuro"},{"key":"bib75","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1038\/382080a0","type":"journal-article","article-title":"Characterization of a multicomponent receptor for GDNF","volume":"382","author":"Treanor","year":"1996","journal-title":"Nature"},{"key":"bib76","doi-asserted-by":"publisher","first-page":"785","DOI":"10.1038\/381785a0","type":"journal-article","article-title":"Functional receptor for GDNF encoded by the c-ret proto-oncogene","volume":"381","author":"Trupp","year":"1996","journal-title":"Nature"},{"key":"bib77","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1038\/ncb2014","type":"journal-article","article-title":"Mesenchymal progenitors distinct from satellite cells contribute to ectopic fat cell formation in skeletal muscle","volume":"12","author":"Uezumi","year":"2010","journal-title":"Nature Cell Biology"},{"key":"bib78","doi-asserted-by":"publisher","first-page":"3654","DOI":"10.1242\/jcs.086629","type":"journal-article","article-title":"Fibrosis and adipogenesis originate from a common mesenchymal progenitor in skeletal muscle","volume":"124","author":"Uezumi","year":"2011","journal-title":"Journal of Cell Science"},{"key":"bib79","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2014.00068","type":"journal-article","article-title":"Roles of nonmyogenic mesenchymal progenitors in pathogenesis and regeneration of skeletal muscle","volume":"5","author":"Uezumi","year":"2014","journal-title":"Frontiers in Physiology"},{"key":"bib80","doi-asserted-by":"publisher","DOI":"10.1172\/JCI139617","type":"journal-article","article-title":"Mesenchymal Bmp3b expression maintains skeletal muscle integrity and decreases in age-related sarcopenia","volume":"131","author":"Uezumi","year":"2021","journal-title":"The Journal of Clinical Investigation"},{"key":"bib81","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-01120-3","type":"journal-article","article-title":"Odd skipped-related 1 identifies a population of embryonic fibro-adipogenic progenitors regulating myogenesis during limb development","volume":"8","author":"Vallecillo-Garc\u00eda","year":"2017","journal-title":"Nature Communications"},{"key":"bib82","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1111\/j.1529-8027.2000.00026.x","type":"journal-article","article-title":"Influence of aging on peripheral nerve function and regeneration","volume":"5","author":"Verd\u00fa","year":"2000","journal-title":"Journal of the Peripheral Nervous System"},{"key":"bib83","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.62232","type":"journal-article","article-title":"Failures of nerve regeneration caused by aging or chronic denervation are rescued by restoring Schwann cell c-Jun","volume":"10","author":"Wagstaff","year":"2021","journal-title":"eLife"},{"key":"bib84","doi-asserted-by":"publisher","first-page":"5002","DOI":"10.1523\/JNEUROSCI.1411-11.2012","type":"journal-article","article-title":"Cooperative roles of BDNF expression in neurons and Schwann cells are modulated by exercise to facilitate nerve regeneration","volume":"32","author":"Wilhelm","year":"2012","journal-title":"The Journal of Neuroscience"},{"key":"bib85","doi-asserted-by":"publisher","first-page":"2029","DOI":"10.1016\/j.celrep.2019.04.074","type":"journal-article","article-title":"Mesenchymal stromal cells are required for regeneration and homeostatic maintenance of skeletal muscle","volume":"27","author":"Wosczyna","year":"2019","journal-title":"Cell Reports"},{"key":"bib86","doi-asserted-by":"publisher","first-page":"4016","DOI":"10.1523\/JNEUROSCI.3811-08.2009","type":"journal-article","article-title":"BDNF exerts contrasting effects on peripheral myelination of NGF-dependent and BDNF-dependent DRG neurons","volume":"29","author":"Xiao","year":"2009","journal-title":"The Journal of Neuroscience"},{"key":"bib87","doi-asserted-by":"publisher","first-page":"1029","DOI":"10.1002\/glia.22491","type":"journal-article","article-title":"Nerve injury induces glial cell line-derived neurotrophic factor (GDNF) expression in Schwann cells through purinergic signaling and the PKC-PKD pathway","volume":"61","author":"Xu","year":"2013","journal-title":"Glia"},{"key":"bib88","doi-asserted-by":"crossref","first-page":"4171","DOI":"10.1111\/j.1460-9568.2000.01312.x","type":"journal-article","article-title":"Endogenous BDNF is required for myelination and regeneration of injured sciatic nerve in rodents","volume":"12","author":"Zhang","year":"2000","journal-title":"The European Journal of Neuroscience"},{"key":"bib89","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.80881","type":"journal-article","article-title":"The injured sciatic nerve atlas (iSNAT), insights into the cellular and molecular basis of neural tissue degeneration and regeneration","volume":"11","author":"Zhao","year":"2022","journal-title":"eLife"},{"key":"bib90","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.neulet.2016.04.006","type":"journal-article","article-title":"BDNF promotes the axonal regrowth after sciatic nerve crush through intrinsic neuronal capability upregulation and distal portion protection","volume":"621","author":"Zheng","year":"2016","journal-title":"Neuroscience Letters"},{"key":"dataset1","type":"dataset","article-title":"Muscle-resident mesenchymal progenitors sense and repair peripheral nerve injury via the GDNF-BDNF axis","volume-title":"NCBI Gene Expression Omnibus","author":"Yoo","year":"2024","unstructured":"Yoo K, Kong Y-Y. 2024. Muscle-resident mesenchymal progenitors sense and repair peripheral nerve injury via the GDNF-BDNF axis. NCBI Gene Expression Omnibus. https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE250436. GSE250436."},{"key":"dataset2","type":"dataset","article-title":"Mesenchymal precursor cells in adult nerves contribute to mammalian tissue repair and regeneration","volume-title":"NCBI Gene Expression Omnibus","author":"Carr","year":"2019","unstructured":"Carr MJ, Toma JS, Johnston AP, Steadman PE, Yuzwa SA, Mahmud N, Frankland PW, Kaplan DR, Miller FD. 2019. Mesenchymal precursor cells in adult nerves contribute to mammalian tissue repair and regeneration. NCBI Gene Expression Omnibus. https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE120678. GSE120678."},{"key":"dataset3","type":"dataset","article-title":"Peripheral Nerve Single-Cell Analysis Identifies Mesenchymal Ligands that Promote Axonal Growth (scRNAseq data)","volume-title":"NCBI Gene Expression Omnibus","author":"Toma","year":"2020","unstructured":"Toma JS, Karamboulas K, Carr MJ, Kolaj A, Yuzwa SA, Mahmud N, Storer MA, Kaplan DR, Miller FD. 2020. Peripheral Nerve Single-Cell Analysis Identifies Mesenchymal Ligands that Promote Axonal Growth (scRNAseq data). NCBI Gene Expression Omnibus. https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE147285. GSE147285."},{"key":"dataset4","type":"dataset","article-title":"Injured Sciatic Nerve Atlas (iSNAT)","volume-title":"NCBI Gene Expression Omnibus","author":"Giger","year":"2022","unstructured":"Giger RJ, Johnson CN, Zhao X. 2022. Injured Sciatic Nerve Atlas (iSNAT). NCBI Gene Expression Omnibus. https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE198582. GSE198582."},{"key":"dataset5","type":"dataset","article-title":"Muscle denervation promotes functional interactions between glial and mesenchymal cells through NGFR and NGF","volume-title":"NCBI Gene Expression Omnibus","author":"Puri","year":"2023","unstructured":"Puri PL, Nicoletti C, Wei X. 2023. Muscle denervation promotes functional interactions between glial and mesenchymal cells through NGFR and NGF. NCBI Gene Expression Omnibus. https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE221736. GSE221736."}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/97662\/elife-97662-v1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/97662\/elife-97662-v1.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/97662","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:12:47Z","timestamp":1787566367000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/97662"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,26]]},"references-count":95,"alternative-id":["10.7554\/eLife.97662"],"URL":"https:\/\/doi.org\/10.7554\/elife.97662","archive":["CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.03.25.586563","asserted-by":"subject"}],"references":[{"id-type":"accession","id":"GSE120678","asserted-by":"subject"},{"id-type":"accession","id":"GSE147285","asserted-by":"subject"},{"id-type":"accession","id":"GSE198582","asserted-by":"subject"},{"id-type":"accession","id":"GSE221736","asserted-by":"subject"}],"is-same-as":[{"id-type":"doi","id":"10.7554\/eLife.97662.3","asserted-by":"subject"}],"is-version-of":[{"id-type":"doi","id":"10.7554\/eLife.97662.1","asserted-by":"subject"},{"id-type":"doi","id":"10.7554\/eLife.97662.2","asserted-by":"subject"}],"is-supplemented-by":[{"id-type":"accession","id":"GSE250436","asserted-by":"subject"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,26]]},"assertion":[{"value":"single anonymised","name":"peer_review_transparency","label":"Peer review transparency","group":{"name":"peer_review_taxonomy"}},{"value":"other reviewer(s), editor","name":"peer_review_interaction","label":"Peer review interaction","group":{"name":"peer_review_taxonomy"}},{"value":"review summaries, review reports, author\/editor communication, reviewer identities reviewer opt in, editor identities","name":"peer_review_published","label":"Peer review published","group":{"name":"peer_review_taxonomy"}},{"value":"open (sign in with ORCID iD required)","name":"post_publication_commenting","label":"Post publication commenting","group":{"name":"post_publication_commenting"}}],"article-number":"RP97662"}}