{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:35:33Z","timestamp":1787567733159,"version":"build-2736575974"},"reference-count":62,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T00:00:00Z","timestamp":1747008000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T00:00:00Z","timestamp":1747008000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,12]],"date-time":"2025-05-12T00:00:00Z","timestamp":1747008000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32373093"],"award-info":[{"award-number":["32373093"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32402947"],"award-info":[{"award-number":["32402947"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009010","name":"Henan Agricultural University","doi-asserted-by":"publisher","award":["30500946"],"award-info":[{"award-number":["30500946"]}],"id":[{"id":"10.13039\/501100009010","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30900880"],"award-info":[{"award-number":["30900880"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Rtf1 is generally considered to be a subunit of the Paf1 complex (Paf1C), which is a multifunctional protein complex involved in histone modification and RNA biosynthesis at multiple stages. Rtf1 is stably associated with the Paf1C in\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    , but not in other species including humans. Little is known about its function in human fungal pathogens. Here, we show that Rtf1 is required for facilitating H2B monoubiquitination (H2Bub1), and regulates fungal morphogenesis and pathogenicity in the meningitis-causing fungal pathogen\n                    <jats:italic>Cryptococcus neoformans<\/jats:italic>\n                    . Rtf1 is not tightly associated with the Paf1C, and its histone modification domain (HMD) is sufficient to promote H2Bub1 and the expression of genes related to fungal mating and filamentation. Moreover, Rtf1 HMD fully restores fungal morphogenesis and pathogenicity; however, it fails to restore defects of thermal tolerance and melanin production in the\n                    <jats:italic>rtf1<\/jats:italic>\n                    \u0394 strain background. The present study establishes a role for cryptococcal Rtf1 as a Paf1C-independent regulator in regulating fungal morphogenesis and pathogenicity, and highlights the function of HMD in facilitating global H2Bub1 in\n                    <jats:italic>C. neoformans<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.7554\/elife.99229","type":"journal-article","created":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T12:29:37Z","timestamp":1722947377000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.99229","source":"Crossref","is-referenced-by-count":0,"title":["Rtf1 HMD domain facilitates global histone H2B monoubiquitination and regulates morphogenesis and virulence in the meningitis-causing pathogen Cryptococcus neoformans"],"prefix":"10.7554","volume":"13","author":[{"given":"Yixuan","family":"Jiang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04eq83d71","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Veterinary Medicine, Henan Agricultural University","place":["Zhengzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"given":"Ying","family":"Liang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04eq83d71","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Veterinary Medicine, Henan Agricultural University","place":["Zhengzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Fujie","family":"Zhao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04eq83d71","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Veterinary Medicine, Henan Agricultural University","place":["Zhengzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"data-curation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Zhenguo","family":"Lu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04eq83d71","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Veterinary Medicine, Henan Agricultural University","place":["Zhengzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"data-curation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Siyu","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04eq83d71","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Veterinary Medicine, Henan Agricultural University","place":["Zhengzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"data-curation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Yao","family":"Meng","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04eq83d71","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Veterinary Medicine, Henan Agricultural University","place":["Zhengzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"data-curation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Zhanxiang","family":"Liu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04eq83d71","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Veterinary Medicine, Henan Agricultural University","place":["Zhengzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"data-curation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04eq83d71","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Veterinary Medicine, Henan Agricultural University","place":["Zhengzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"corresponding-author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2406-3922","authenticated-orcid":true,"given":"Youbao","family":"Zhao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04eq83d71","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Veterinary Medicine, Henan Agricultural University","place":["Zhengzhou, China"]},{"id":[{"id":"https:\/\/ror.org\/05ckt8b96","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of Quality and Safety Control of Poultry Products, Ministry of Agriculture and Rural Affairs","place":["Zhengzhou, China"]},{"name":"Henan Province Key Laboratory of Animal Food Pathogens Surveillance","place":["Zhengzhou, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"corresponding-author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"4374","published-online":{"date-parts":[[2025,5,12]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"2254","DOI":"10.1101\/gad.177238.111","type":"journal-article","article-title":"Genome-wide function of H2B ubiquitylation in promoter and genic regions","volume":"25","author":"Batta","year":"2011","journal-title":"Genes & Development"},{"key":"bib2","doi-asserted-by":"publisher","DOI":"10.1038\/nature00970","type":"journal-article","article-title":"Gene silencing: trans-histone regulatory pathway in chromatin","volume":"418","author":"Briggs","year":"2002","journal-title":"Nature"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"2765","DOI":"10.1101\/gad.1834709","type":"journal-article","article-title":"DSIF, the Paf1 complex, and Tat-SF1 have nonredundant, cooperative roles in RNA polymerase II elongation","volume":"23","author":"Chen","year":"2009","journal-title":"Genes & Development"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"336","DOI":"10.1016\/j.cels.2020.08.009","type":"journal-article","article-title":"Optogenetic control reveals differential promoter interpretation of transcription factor nuclear translocation dynamics","volume":"11","author":"Chen","year":"2020","journal-title":"Cell Systems"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"10619","DOI":"10.1093\/nar\/gkt819","type":"journal-article","article-title":"Structural insights into Paf1 complex assembly and histone binding","volume":"41","author":"Chu","year":"2013","journal-title":"Nucleic Acids Research"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1093\/genetics\/156.2.535","type":"journal-article","article-title":"Synthetic lethal interactions suggest a role for the Saccharomyces cerevisiae Rtf1 protein in transcription elongation","volume":"156","author":"Costa","year":"2000","journal-title":"Genetics"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1016\/j.str.2007.10.018","type":"journal-article","article-title":"Structure and DNA binding of the human Rtf1 Plus3 domain","volume":"16","author":"de Jong","year":"2008","journal-title":"Structure"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"28368","DOI":"10.1074\/jbc.C200348200","type":"journal-article","article-title":"Methylation of histone H3 by COMPASS requires ubiquitination of histone H2B by Rad6","volume":"277","author":"Dover","year":"2002","journal-title":"The Journal of Biological Chemistry"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"1357","DOI":"10.1534\/genetics.117.300656","type":"journal-article","article-title":"Multiple applications of a transient CRISPR-Cas9 coupled with electroporation (TRACE) system in the Cryptococcus neoformans species complex","volume":"208","author":"Fan","year":"2018","journal-title":"Genetics"},{"key":"bib10","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2020.103464","type":"journal-article","article-title":"An intergenic \u201csafe haven\u201d region in Cryptococcus neoformans serotype D genomes","volume":"144","author":"Fan","year":"2020","journal-title":"Fungal Genetics and Biology"},{"key":"bib11","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2220041120","type":"journal-article","article-title":"Paf1 complex subunit Rtf1 stimulates H2B ubiquitylation by interacting with the highly conserved N-terminal helix of Rad6","volume":"120","author":"Fetian","year":"2023","journal-title":"PNAS"},{"key":"bib12","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2021.166979","type":"journal-article","article-title":"The Paf1 Complex: a keystone of nuclear regulation operating at the interface of transcription and chromatin","volume":"433","author":"Francette","year":"2021","journal-title":"Journal of Molecular Biology"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"14195","DOI":"10.1074\/jbc.M009942200","type":"journal-article","article-title":"Mechanisms governing subcellular localization and function of human RGS2","volume":"276","author":"Heximer","year":"2001","journal-title":"The Journal of Biological Chemistry"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1128\/EC.4.1.147-155.2005","type":"journal-article","article-title":"A homolog of Ste6, the A-factor transporter in Saccharomyces cerevisiae, is required for mating but not for monokaryotic fruiting in Cryptococcus neoformans","volume":"4","author":"Hsueh","year":"2005","journal-title":"Eukaryotic Cell"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1016\/s1097-2765(02)00826-2","type":"journal-article","article-title":"A conserved RING finger protein required for histone H2B monoubiquitination and cell size control","volume":"11","author":"Hwang","year":"2003","journal-title":"Molecular Cell"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"454","DOI":"10.1038\/s41579-021-00511-0","type":"journal-article","article-title":"Treatment strategies for cryptococcal infection: challenges, advances and future outlook","volume":"19","author":"Iyer","year":"2021","journal-title":"Nature Reviews. Microbiology"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1016\/j.bbagrm.2010.01.001","type":"journal-article","article-title":"The Paf1 complex: platform or player in RNA polymerase II transcription?","volume":"1799","author":"Jaehning","year":"2010","journal-title":"Biochimica et Biophysica Acta"},{"key":"bib18","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1016\/j.cell.2009.02.045","type":"journal-article","article-title":"Dimethylation of H3K4 by Set1 recruits the Set3 histone deacetylase complex to 5\u2019 transcribed regions","volume":"137","author":"Kim","year":"2009","journal-title":"Cell"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"459","DOI":"10.1016\/j.cell.2009.02.027","type":"journal-article","article-title":"RAD6-Mediated transcription-coupled H2B ubiquitylation directly stimulates H3K4 methylation in human cells","volume":"137","author":"Kim","year":"2009","journal-title":"Cell"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1016\/j.cell.2009.12.050","type":"journal-article","article-title":"The human PAF1 complex acts in chromatin transcription elongation both independently and cooperatively with SII\/TFIIS","volume":"140","author":"Kim","year":"2010","journal-title":"Cell"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"1158","DOI":"10.1016\/j.cell.2012.08.016","type":"journal-article","article-title":"Set3 HDAC mediates effects of overlapping noncoding transcription on gene induction kinetics","volume":"150","author":"Kim","year":"2012","journal-title":"Cell"},{"key":"bib22","doi-asserted-by":"publisher","first-page":"2126","DOI":"10.1093\/nar\/27.10.2126","type":"journal-article","article-title":"A role for Ctr9p and Paf1p in the regulation G1 cyclin expression in yeast","volume":"27","author":"Koch","year":"1999","journal-title":"Nucleic Acids Research"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"721","DOI":"10.1016\/s1097-2765(03)00091-1","type":"journal-article","article-title":"The Paf1 complex is required for histone H3 methylation by COMPASS and Dot1p: linking transcriptional elongation to histone methylation","volume":"11","author":"Krogan","year":"2003","journal-title":"Molecular Cell"},{"key":"bib24","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1146\/annurev.micro.60.080805.142102","type":"journal-article","article-title":"The biology of the Cryptococcus neoformans species complex","volume":"60","author":"Lin","year":"2006","journal-title":"Annual Review of Microbiology"},{"key":"bib25","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000953","type":"journal-article","article-title":"Transcription factors Mat2 and Znf2 operate cellular circuits orchestrating opposite- and same-sex mating in Cryptococcus neoformans","volume":"6","author":"Lin","year":"2010","journal-title":"PLOS Genetics"},{"key":"bib26","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2020.103364","type":"journal-article","article-title":"Transformation of Cryptococcus neoformans by electroporation using a transient CRISPR-Cas9 expression (TRACE) system","volume":"138","author":"Lin","year":"2020","journal-title":"Fungal Genetics and Biology"},{"key":"bib27","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02785-21","type":"journal-article","article-title":"Immunoprotection against cryptococcosis offered by Znf2 depends on capsule and the hyphal morphology","volume":"13","author":"Lin","year":"2022","journal-title":"mBio"},{"key":"bib28","doi-asserted-by":"publisher","DOI":"10.3390\/jof9060672","type":"journal-article","article-title":"The COMPASS complex regulates fungal development and virulence through histone crosstalk in the fungal pathogen cryptococcus neoformans","volume":"9","author":"Liu","year":"2023","journal-title":"Journal of Fungi"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1038\/nrmicro.2015.6","type":"journal-article","article-title":"Cryptococcus: from environmental saprophyte to global pathogen","volume":"14","author":"May","year":"2016","journal-title":"Nature Reviews. Microbiology"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"1971","DOI":"10.1128\/MCB.22.7.1971-1980.2002","type":"journal-article","article-title":"Ctr9, Rtf1, and Leo1 are components of the Paf1\/RNA polymerase II complex","volume":"22","author":"Mueller","year":"2002","journal-title":"Molecular and Cellular Biology"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1016\/s1097-2765(04)00257-6","type":"journal-article","article-title":"The Paf1 complex has functions independent of actively transcribing RNA polymerase II","volume":"14","author":"Mueller","year":"2004","journal-title":"Molecular Cell"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"33625","DOI":"10.1074\/jbc.C300270200","type":"journal-article","article-title":"The Rtf1 component of the Paf1 transcriptional elongation complex is required for ubiquitination of histone H2B","volume":"278","author":"Ng","year":"2003","journal-title":"The Journal of Biological Chemistry"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1016\/j.molcel.2005.08.023","type":"journal-article","article-title":"A posttranscriptional role for the yeast Paf1-RNA polymerase II complex is revealed by identification of primary targets","volume":"20","author":"Penheiter","year":"2005","journal-title":"Molecular Cell"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"10837","DOI":"10.1073\/pnas.1116994109","type":"journal-article","article-title":"Small region of Rtf1 protein can substitute for complete Paf1 complex in facilitating global histone H2B ubiquitylation in yeast","volume":"109","author":"Piro","year":"2012","journal-title":"PNAS"},{"key":"bib35","doi-asserted-by":"publisher","first-page":"1748","DOI":"10.1016\/S1473-3099(22)00499-6","type":"journal-article","article-title":"The global burden of HIV-associated cryptococcal infection in adults in 2020: a modelling analysis","volume":"22","author":"Rajasingham","year":"2022","journal-title":"The Lancet. Infectious Diseases"},{"key":"bib36","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1126\/science.287.5452.501","type":"journal-article","article-title":"Rad6-dependent ubiquitination of histone H2B in yeast","volume":"287","author":"Robzyk","year":"2000","journal-title":"Science"},{"key":"bib37","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1073\/pnas.0812023106","type":"journal-article","article-title":"The tumor suppressor Cdc73 functionally associates with CPSF and CstF 3\u2019 mRNA processing factors","volume":"106","author":"Rozenblatt-Rosen","year":"2009","journal-title":"PNAS"},{"key":"bib38","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1016\/j.molcel.2005.08.026","type":"journal-article","article-title":"A Requirement for the Saccharomyces cerevisiae Paf1 complex in snoRNA 3\u2019 end formation","volume":"20","author":"Sheldon","year":"2005","journal-title":"Molecular Cell"},{"key":"bib39","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1128\/EC.1.3.366-377.2002","type":"journal-article","article-title":"Pheromones stimulate mating and differentiation via paracrine and autocrine signaling in Cryptococcus neoformans","volume":"1","author":"Shen","year":"2002","journal-title":"Eukaryotic Cell"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"1764","DOI":"10.1093\/emboj\/21.7.1764","type":"journal-article","article-title":"The Paf1 complex physically and functionally associates with transcription elongation factors in vivo","volume":"21","author":"Squazzo","year":"2002","journal-title":"The EMBO Journal"},{"key":"bib41","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1038\/nature00883","type":"journal-article","article-title":"Ubiquitination of histone H2B regulates H3 methylation and gene silencing in yeast","volume":"418","author":"Sun","year":"2002","journal-title":"Nature"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1042\/BCJ20200170","type":"journal-article","article-title":"Combinations of histone post-translational modifications","volume":"478","author":"Taylor","year":"2021","journal-title":"The Biochemical Journal"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1534\/genetics.111.128645","type":"journal-article","article-title":"Identification of a role for histone H2B ubiquitylation in noncoding RNA 3\u2019-end formation through mutational analysis of Rtf1 in Saccharomyces cerevisiae","volume":"188","author":"Tomson","year":"2011","journal-title":"Genetics"},{"key":"bib44","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.fgb.2017.08.008","type":"journal-article","article-title":"A fluorogenic C. neoformans reporter strain with A robust expression of m-cherry expressed from A safe haven site in the genome","volume":"108","author":"Upadhya","year":"2017","journal-title":"Fungal Genetics and Biology"},{"key":"bib45","doi-asserted-by":"publisher","first-page":"815","DOI":"10.1016\/j.molcel.2016.10.008","type":"journal-article","article-title":"The histone modification domain of paf1 complex subunit rtf1 directly stimulates h2b ubiquitylation through an interaction with Rad6","volume":"64","author":"Van Oss","year":"2016","journal-title":"Molecular Cell"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1093\/infdis\/167.1.186","type":"journal-article","article-title":"Regulation of cryptococcal capsular polysaccharide by iron","volume":"167","author":"Vartivarian","year":"1993","journal-title":"The Journal of Infectious Diseases"},{"key":"bib47","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1038\/s41586-018-0440-4","type":"journal-article","article-title":"Structure of activated transcription complex Pol II-DSIF-PAF-SPT6","volume":"560","author":"Vos","year":"2018","journal-title":"Nature"},{"key":"bib48","doi-asserted-by":"publisher","first-page":"358","DOI":"10.1016\/S1369-5274(99)80063-0","type":"journal-article","article-title":"Signal transduction cascades regulating mating, filamentation, and virulence in Cryptococcus neoformans","volume":"2","author":"Wang","year":"1999","journal-title":"Current Opinion in Microbiology"},{"key":"bib49","doi-asserted-by":"publisher","first-page":"11571","DOI":"10.1073\/pnas.1308173110","type":"journal-article","article-title":"Fungal adhesion protein guides community behaviors and autoinduction in a paracrine manner","volume":"110","author":"Wang","year":"2013","journal-title":"PNAS"},{"key":"bib50","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1016\/s1097-2765(02)00802-x","type":"journal-article","article-title":"Bre1, an E3 ubiquitin ligase required for recruitment and substrate selection of Rad6 at a promoter","volume":"11","author":"Wood","year":"2003","journal-title":"Molecular Cell"},{"key":"bib51","doi-asserted-by":"publisher","first-page":"34739","DOI":"10.1074\/jbc.C300269200","type":"journal-article","article-title":"The Paf1 complex is essential for histone monoubiquitination by the Rad6-Bre1 complex, which signals for histone methylation by COMPASS and Dot1p","volume":"278","author":"Wood","year":"2003","journal-title":"The Journal of Biological Chemistry"},{"key":"bib52","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1016\/j.sbi.2019.05.009","type":"journal-article","article-title":"Activation and regulation of H2B-Ubiquitin-dependent histone methyltransferases","volume":"59","author":"Worden","year":"2019","journal-title":"Current Opinion in Structural Biology"},{"key":"bib53","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1169","type":"journal-article","article-title":"T5 exonuclease-dependent assembly offers a low-cost method for efficient cloning and site-directed mutagenesis","volume":"47","author":"Xia","year":"2019","journal-title":"Nucleic Acids Research"},{"key":"bib54","doi-asserted-by":"publisher","first-page":"564","DOI":"10.1038\/cr.2011.42","type":"journal-article","article-title":"Readers of histone modifications","volume":"21","author":"Yun","year":"2011","journal-title":"Cell Research"},{"key":"bib55","doi-asserted-by":"publisher","first-page":"2626","DOI":"10.1128\/IAI.00259-13","type":"journal-article","article-title":"Congenic strains of the filamentous form of Cryptococcus neoformans for studies of fungal morphogenesis and virulence","volume":"81","author":"Zhai","year":"2013","journal-title":"Infection and Immunity"},{"key":"bib56","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02135-18","type":"journal-article","article-title":"PAS domain protein PDomain Pras3 interacts with the chromatin modifier Bre1 in regulating cryptococcal morphogenesis","volume":"9","author":"Zhao","year":"2018","journal-title":"mBio"},{"key":"bib57","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1146\/annurev-micro-020518-120210","type":"journal-article","article-title":"Life cycle of cryptococcus neoformans","volume":"73","author":"Zhao","year":"2019","journal-title":"Annual Review of Microbiology"},{"key":"bib58","doi-asserted-by":"publisher","first-page":"1387","DOI":"10.1016\/j.cub.2020.01.081","type":"journal-article","article-title":"Activation of meiotic genes mediates ploidy reduction during cryptococcal infection","volume":"30","author":"Zhao","year":"2020","journal-title":"Current Biology"},{"key":"bib59","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.03602-20","type":"journal-article","article-title":"A PAS protein directs metabolic reprogramming during cryptococcal adaptation to hypoxia","volume":"12","author":"Zhao","year":"2021","journal-title":"mBio"},{"key":"bib60","doi-asserted-by":"publisher","DOI":"10.1186\/s40249-023-01073-4","type":"journal-article","article-title":"Cryptococcus neoformans, a global threat to human health","volume":"12","author":"Zhao","year":"2023","journal-title":"Infectious Diseases of Poverty"},{"key":"bib61","doi-asserted-by":"publisher","first-page":"1668","DOI":"10.1101\/gad.1292105","type":"journal-article","article-title":"The human PAF complex coordinates transcription with events downstream of RNA synthesis","volume":"19","author":"Zhu","year":"2005","journal-title":"Genes & Development"},{"key":"dataset1","type":"dataset","article-title":"Rtf1 HMD domain facilitates global histone H2B monoubiquitination and regulates morphogenesis and virulence in the meningitis-causing pathogen Cryptococcus neoformans","volume-title":"NCBI Gene Expression Omnibus","author":"Jiang","year":"2025","unstructured":"Jiang Y, Liang Y, Zhang J, Zhao Y. 2025. Rtf1 HMD domain facilitates global histone H2B monoubiquitination and regulates morphogenesis and virulence in the meningitis-causing pathogen Cryptococcus neoformans. NCBI Gene Expression Omnibus. https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE296031. GSE296031."}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/99229\/elife-99229-v1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/99229\/elife-99229-v1.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/99229","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T09:38:21Z","timestamp":1787564301000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/99229"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,12]]},"references-count":62,"alternative-id":["10.7554\/eLife.99229"],"URL":"https:\/\/doi.org\/10.7554\/elife.99229","archive":["CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.05.20.594937","asserted-by":"subject"},{"id-type":"doi","id":"10.1101\/2024.05.20.594937","asserted-by":"object"}],"is-same-as":[{"id-type":"doi","id":"10.7554\/eLife.99229.3","asserted-by":"subject"}],"is-version-of":[{"id-type":"doi","id":"10.7554\/eLife.99229.1","asserted-by":"subject"},{"id-type":"doi","id":"10.7554\/eLife.99229.2","asserted-by":"subject"}],"is-supplemented-by":[{"id-type":"accession","id":"GSE296031","asserted-by":"subject"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,12]]},"assertion":[{"value":"single anonymised","name":"peer_review_transparency","label":"Peer review transparency","group":{"name":"peer_review_taxonomy"}},{"value":"other reviewer(s), editor","name":"peer_review_interaction","label":"Peer review interaction","group":{"name":"peer_review_taxonomy"}},{"value":"review summaries, review reports, author\/editor communication, reviewer identities reviewer opt in, editor identities","name":"peer_review_published","label":"Peer review published","group":{"name":"peer_review_taxonomy"}},{"value":"open (sign in with ORCID iD required)","name":"post_publication_commenting","label":"Post publication commenting","group":{"name":"post_publication_commenting"}}],"article-number":"RP99229"}}