{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:34:56Z","timestamp":1787567696520,"version":"build-2736575974"},"reference-count":61,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T00:00:00Z","timestamp":1750118400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T00:00:00Z","timestamp":1750118400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T00:00:00Z","timestamp":1750118400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018537","name":"National Science and Technology Major Project","doi-asserted-by":"publisher","award":["Prevention and Treatment of Infectious Diseases (2018ZX10101003-005-005)"],"award-info":[{"award-number":["Prevention and Treatment of Infectious Diseases (2018ZX10101003-005-005)"]}],"id":[{"id":"10.13039\/501100018537","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82203636"],"award-info":[{"award-number":["82203636"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Young Elite Scientist Sponsorship Program by CAST","award":["2022QNRC001"],"award-info":[{"award-number":["2022QNRC001"]}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Most of the human gastric cancer (GC) worldwide are ascribed to\n                    <jats:italic>Helicobacter pylori<\/jats:italic>\n                    infections, which have a detrimental effect on the immunotherapy\u2019s efficacy. Comprehensively dissecting the key cell players and molecular pathways associated with cancer immunotherapies is critical for developing novel therapeutic strategies against\n                    <jats:italic>H. pylori<\/jats:italic>\n                    infection-associated human GC. We performed a comprehensive single-cell transcriptome analysis of nine GC patients with current\n                    <jats:italic>H. pylori<\/jats:italic>\n                    infection (HpGC), three GC patients with previous\n                    <jats:italic>H. pylori<\/jats:italic>\n                    infection (ex-HpGC), six GC patients without\n                    <jats:italic>H. pylori<\/jats:italic>\n                    infection (non-HpGC), and six healthy controls (HC). We also investigated key cell players and molecular pathways associated with GC immunotherapy outcomes. We revealed the molecular heterogeneity of different cell components in GC, including epithelium, immune cells, and cancer-associated fibroblasts (CAFs) at the single-cell level. The malignant epithelium of HpGC exhibited high expression level of inflammatory and epithelial\u2013mesenchymal transition signature, HpGC and ex-HpGC were enriched with VEGFA+ angiogenic tumor-associated macrophages (Angio-TAM) and IL11+ inflammatory CAF (iCAF), characterized by high expression levels of NECTIN2 and VEGFA\/B. Additionally, we found significant correlations between the abundance of iCAF with Angio-TAM and TIGIT+ suppressive T cells, and iCAF interacted with Angio-TAM through the VEGF and ANGPTL angiogenic pathways. We also developed an immune signature and angiogenic signature and demonstrated that the iCAF abundance and angiogenic signature could predict poor immunotherapy outcomes in GC. We revealed the transcriptome characteristics and heterogeneity of various cellular constituents of HpGC patients and demonstrated that a synergistic combination of immunotherapy and anti-angiogenic targeted therapy may be an effective therapeutic modality for HpGC patients.\n                  <\/jats:p>","DOI":"10.7554\/elife.99337","type":"journal-article","created":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T10:26:17Z","timestamp":1725272777000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.99337","source":"Crossref","is-referenced-by-count":3,"title":["Single-cell dissection of prognostic architecture and immunotherap response in Helicobacter pylori infection-associated gastric cancer"],"prefix":"10.7554","volume":"13","author":[{"given":"Xin","family":"Zhang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02bv3c993","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Pathogen and Biosecurity, Institute of Biotechnology, 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