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Central to this is the \u03b2-barrel assembly machine (Bam) complex and its associated chaperones, which are responsible for transport, folding, and insertion of outer membrane proteins (OMPs). The\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    Bam complex is composed of two essential subunits, BamA and BamD, and three non-essential accessory lipoproteins, BamB, BamC, and BamE. Optimal Bam function is further dependent on the non-essential periplasmic chaperones DegP, Skp, and SurA. Despite intensive study, the specific function of these non-essential Bam-associated proteins is not fully understood. Here, we analysed \u0394\n                    <jats:italic>bamB<\/jats:italic>\n                    , \u0394\n                    <jats:italic>bamC<\/jats:italic>\n                    , \u0394\n                    <jats:italic>bamE<\/jats:italic>\n                    , \u0394\n                    <jats:italic>surA<\/jats:italic>\n                    , \u0394\n                    <jats:italic>skp,<\/jats:italic>\n                    and \u0394\n                    <jats:italic>degP<\/jats:italic>\n                    knockout strains by phenotypic screening, conservation analysis and high-throughput genetics. We identified hundreds of synthetic-lethal interactions and revealed that Bam complex activity is impacted by changes in outer membrane lipid composition and that enterobacterial common antigen is essential in the absence of the chaperone SurA. We also show that genes responsible for synthesis of peptidoglycan are synthetically lethal with Bam accessory lipoprotein encoding genes. Together, our data indicate potential mechanisms for coordination of OMP biogenesis with other cellular growth processes, such as LPS and peptidoglycan biogenesis.\n                  <\/jats:p>","DOI":"10.7554\/elife.99955","type":"journal-article","created":{"date-parts":[[2024,9,3]],"date-time":"2024-09-03T12:28:00Z","timestamp":1725366480000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.99955","source":"Crossref","is-referenced-by-count":0,"title":["Genome-wide synthetic lethality screen of Bam complex-associated genes in Escherichia coli"],"prefix":"10.7554","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7912-2144","authenticated-orcid":true,"given":"Jack A","family":"Bryant","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03angcq70","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Microbiology and Infection, School of Biosciences, University of Birmingham","place":["Edgbaston, United 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