{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T19:17:06Z","timestamp":1791573426319,"version":"4.3.4"},"reference-count":48,"publisher":"Journal of Biomedical Research","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomed Res"],"published-print":{"date-parts":[[2026]]},"DOI":"10.7555\/jbr.39.20250189","type":"journal-article","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T11:07:47Z","timestamp":1756379267000},"page":"312","source":"Crossref","is-referenced-by-count":4,"title":["hUMSC-derived exosomes alleviate hypoxic cerebrovascular injury &lt;i&gt;via&lt;\/i&gt; AMPK\/NLRP3-mediated pyroptosis suppression and mitochondrial protection"],"prefix":"10.7555","volume":"40","author":[{"given":"Jinteng","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunlong","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haolin","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingyun","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingyue","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingmei","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qibing","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4380","reference":[{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.1","doi-asserted-by":"publisher","unstructured":"Tsao CW, Aday AW, Almarzooq ZI, et al. Heart disease and stroke statistics-2022 update: A report from the American Heart Association[J]. <i>Circulation<\/i>, 2022, 145(8): e153\u2013e639.","DOI":"10.1161\/CIR.0000000000001052"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.2","doi-asserted-by":"publisher","unstructured":"Sacco RL, Kasner SE, Broderick JP, et al. An updated definition of stroke for the 21st century: A statement for healthcare professionals from the American Heart Association\/American Stroke Association[J]. <i>Stroke<\/i>, 2013, 44(7): 2064\u20132089.","DOI":"10.1161\/STR.0b013e318296aeca"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.3","doi-asserted-by":"publisher","unstructured":"Zhao K, Wang P, Tang X, et al. The mechanisms of minocycline in alleviating ischemic stroke damage and cerebral ischemia-reperfusion injury[J]. <i>Eur J Pharmacol<\/i>, 2023, 955: 175903.","DOI":"10.1016\/j.ejphar.2023.175903"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.4","doi-asserted-by":"publisher","unstructured":"Zhang W, Xu M, Chen F, et al. Targeting the JAK2-STAT3 pathway to inhibit cGAS-STING activation improves neuronal senescence after ischemic stroke[J]. <i>Exp Neurol<\/i>, 2023, 368: 114474.","DOI":"10.1016\/j.expneurol.2023.114474"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.5","doi-asserted-by":"publisher","unstructured":"Wang F, Cao Y, Ma L, et al. Dysfunction of cerebrovascular endothelial cells: Prelude to vascular dementia[J]. <i>Front Aging Neurosci<\/i>, 2018, 10: 376.","DOI":"10.3389\/fnagi.2018.00376"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.6","doi-asserted-by":"publisher","unstructured":"Alkayed NJ, Cipolla MJ. Role of endothelial cells and platelets in COVID-related cerebrovascular events[J]. <i>Stroke<\/i>, 2022, 53(7): 2389\u20132392.","DOI":"10.1161\/STROKEAHA.122.039971"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.7","doi-asserted-by":"publisher","unstructured":"Koh YJ, Koh BI, Kim H, et al. Stromal vascular fraction from adipose tissue forms profound vascular network through the dynamic reassembly of blood endothelial cells[J]. <i>Arterioscler Thromb Vasc Biol<\/i>, 2011, 31(5): 1141\u20131150.","DOI":"10.1161\/ATVBAHA.110.218206"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.8","doi-asserted-by":"publisher","unstructured":"Neubauer K, Zieger B. Endothelial cells and coagulation[J]. <i>Cell Tissue Res<\/i>, 2022, 387(3): 391\u2013398.","DOI":"10.1007\/s00441-021-03471-2"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.9","doi-asserted-by":"publisher","unstructured":"Ferreira Tojais N, Peghaire C, Franzl N, et al. Frizzled7 controls vascular permeability through the Wnt-canonical pathway and cross-talk with endothelial cell junction complexes[J]. <i>Cardiovasc Res<\/i>, 2014, 103(2): 291\u2013303.","DOI":"10.1093\/cvr\/cvu133"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.10","doi-asserted-by":"publisher","unstructured":"Nobe K, Sone T, Paul RJ, et al. Thrombin-induced force development in vascular endothelial cells: Contribution to alteration of permeability mediated by calcium-dependent and -independent pathways[J]. <i>J Pharmacol Sci<\/i>, 2005, 99(3): 252\u2013263.","DOI":"10.1254\/jphs.FP0050679"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.11","doi-asserted-by":"publisher","unstructured":"Li G, Gao J, Ding P, et al. The role of endothelial cell-pericyte interactions in vascularization and diseases[J]. <i>J Adv Res<\/i>, 2025, 67: 269\u2013288.","DOI":"10.1016\/j.jare.2024.01.016"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.12","doi-asserted-by":"publisher","unstructured":"Tirandi A, Sgura C, Carbone F, et al. Inflammatory biomarkers of ischemic stroke[J]. <i>Intern Emerg Med<\/i>, 2023, 18(3): 723\u2013732.","DOI":"10.1007\/s11739-023-03201-2"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.13","doi-asserted-by":"publisher","unstructured":"Datta A, Sarmah D, Mounica L, et al. Cell death pathways in ischemic stroke and targeted pharmacotherapy[J]. <i>Transl Stroke Res<\/i>, 2020, 11(6): 1185\u20131202.","DOI":"10.1007\/s12975-020-00806-z"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.14","doi-asserted-by":"publisher","unstructured":"Gong P, Jia H, Li R, et al. Downregulation of Nogo-B ameliorates cerebral ischemia\/reperfusion injury in mice through regulating microglia polarization <i>via<\/i> TLR4\/NF-kappaB pathway[J]. <i>Neurochem Int<\/i>, 2023, 167: 105553.","DOI":"10.1016\/j.neuint.2023.105553"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.15","doi-asserted-by":"publisher","unstructured":"Franke M, Bieber M, Kraft P, et al. The NLRP3 inflammasome drives inflammation in ischemia\/reperfusion injury after transient middle cerebral artery occlusion in mice[J]. <i>Brain, Behav, Immun<\/i>, 2021, 92: 221\u2013231.","DOI":"10.1016\/j.bbi.2020.12.009"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.16","doi-asserted-by":"publisher","unstructured":"Su X, Liu B, Wang S, et al. NLRP3 inflammasome: A potential therapeutic target to minimize renal ischemia\/reperfusion injury during transplantation[J]. <i>Transpl Immunol<\/i>, 2022, 75: 101718.","DOI":"10.1016\/j.trim.2022.101718"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.17","doi-asserted-by":"publisher","unstructured":"Shang D, Liu H, Tu Z. Pro-inflammatory cytokines mediating senescence of vascular endothelial cells in atherosclerosis[J]. <i>Fundam Clin Pharmacol<\/i>, 2023, 37(5): 928\u2013936.","DOI":"10.1111\/fcp.12915"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.18","doi-asserted-by":"publisher","unstructured":"Wang S, Chen Y, Wu C, et al. Trehalose alleviates myocardial ischemia\/reperfusion injury by inhibiting NLRP3-mediated pyroptosis[J]. <i>Appl Biochem Biotechnol<\/i>, 2024, 196(3): 1194\u20131210.","DOI":"10.1007\/s12010-023-04613-8"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.19","doi-asserted-by":"publisher","unstructured":"Wang X, Li X, Liu S, et al. PCSK9 regulates pyroptosis <i>via<\/i> mtDNA damage in chronic myocardial ischemia[J]. <i>Basic Res Cardiol<\/i>, 2020, 115(6): 66.","DOI":"10.1007\/s00395-020-00832-w"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.20","doi-asserted-by":"publisher","unstructured":"Ghafouri-Fard S, Shoorei H, Poornajaf Y, et al. NLRP3: Role in ischemia\/reperfusion injuries[J]. <i>Front Immunol<\/i>, 2022, 13: 926895.","DOI":"10.3389\/fimmu.2022.926895"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.21","doi-asserted-by":"publisher","unstructured":"Ward R, Li W, Abdul Y, et al. NLRP3 inflammasome inhibition with MCC950 improves diabetes-mediated cognitive impairment and vasoneuronal remodeling after ischemia[J]. <i>Pharmacol Res<\/i>, 2019, 142: 237\u2013250.","DOI":"10.1016\/j.phrs.2019.01.035"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.22","doi-asserted-by":"publisher","unstructured":"Ala M. The beneficial effects of mesenchymal stem cells and their exosomes on myocardial infarction and critical considerations for enhancing their efficacy[J]. <i>Ageing Res Rev<\/i>, 2023, 89: 101980.","DOI":"10.1016\/j.arr.2023.101980"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.23","doi-asserted-by":"publisher","unstructured":"Heo JS, Kim S. Human adipose mesenchymal stem cells modulate inflammation and angiogenesis through exosomes[J]. <i>Sci Rep<\/i>, 2022, 12(1): 2776.","DOI":"10.1038\/s41598-022-06824-1"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.24","doi-asserted-by":"publisher","unstructured":"Wu KJ, Yu S, Lee JY, et al. Improving neurorepair in stroke brain through endogenous neurogenesis-enhancing drugs[J]. <i>Cell Transplant<\/i>, 2017, 26(9): 1596\u20131600.","DOI":"10.1177\/0963689717721230"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.25","doi-asserted-by":"publisher","unstructured":"Hua T, Yang M, Song H, et al. Huc-MSCs-derived exosomes attenuate inflammatory pain by regulating microglia pyroptosis and autophagy <i>via<\/i> the miR-146a-5p\/TRAF6 axis[J]. <i>J Nanobiotechnol<\/i>, 2022, 20(1): 324.","DOI":"10.1186\/s12951-022-01522-6"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.26","doi-asserted-by":"publisher","unstructured":"Tang Y, Zhou Y, Li H. Advances in mesenchymal stem cell exosomes: A review[J]. <i>Stem Cell Res Ther<\/i>, 2021, 12(1): 71.","DOI":"10.1186\/s13287-021-02138-7"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.27","doi-asserted-by":"publisher","unstructured":"Zhang P, Guo Z, Xu Y, et al. <i>N<\/i>-Butylphthalide (NBP) ameliorated cerebral ischemia reperfusion-induced brain injury <i>via<\/i> HGF-regulated TLR4\/NF-\u03baB signaling pathway[J]. <i>Biomed Pharmacother<\/i>, 2016, 83: 658\u2013666.","DOI":"10.1016\/j.biopha.2016.07.040"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.28","doi-asserted-by":"publisher","unstructured":"Sun M, Chen J, Liu F, et al. Butylphthalide inhibits ferroptosis and ameliorates cerebral Ischaemia-Reperfusion injury in rats by activating the Nrf2\/HO-1 signalling pathway[J]. <i>Neurotherapeutics<\/i>, 2024, 21(5): e00444.","DOI":"10.1016\/j.neurot.2024.e00444"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.29","doi-asserted-by":"publisher","unstructured":"Huang Y, Pan L, Wu T. Improvement of cerebral ischemia-reperfusion injury by L-3-<i>n<\/i>-butylphthalide through promoting angiogenesis[J]. <i>Exp Brain Res<\/i>, 2021, 239(1): 341\u2013350.","DOI":"10.1007\/s00221-020-05978-6"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.30","doi-asserted-by":"publisher","unstructured":"Lv W, Jiang J, Xu Y, et al. Re-exploring the inflammation-related core genes and modules in cerebral ischemia[J]. <i>Mol Neurobiol<\/i>, 2023, 60(6): 3439\u20133451.","DOI":"10.1007\/s12035-023-03275-1"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.31","doi-asserted-by":"publisher","unstructured":"Shang J, Huang G, Wang B, et al. Shuxuetong injection inhibits pyroptosis in acute ischemic stroke <i>via<\/i> CD44\/NLRP3\/GSDMD signal[J]. <i>J Ethnopharmacol<\/i>, 2025, 345: 119618.","DOI":"10.1016\/j.jep.2025.119618"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.32","doi-asserted-by":"publisher","unstructured":"Wang J, Tang H, Tian J, et al. Extracellular vesicles of ADSCs inhibit ischemic stroke-induced pyroptosis through Gbp3 regulation: A role for the NLRP3\/GSDMD signaling pathway[J]. <i>Int Immunopharmacol<\/i>, 2025, 146: 113881.","DOI":"10.1016\/j.intimp.2024.113881"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.33","doi-asserted-by":"publisher","unstructured":"Ye J, Li D, Jie Y, et al. Exosome-based nanoparticles and cancer immunotherapy[J]. <i>Biomed Pharmacother<\/i>, 2024, 179: 117296.","DOI":"10.1016\/j.biopha.2024.117296"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.34","doi-asserted-by":"publisher","unstructured":"Wang J, Liu Y, Sun W, et al. Plasma exosomes as novel biomarker for the early diagnosis of gastric cancer[J]. <i>Cancer Biomark<\/i>, 2018, 21(4): 805\u2013812.","DOI":"10.3233\/CBM-170738"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.35","doi-asserted-by":"publisher","unstructured":"Li J, Sun S, Zhu D, et al. Inhalable stem cell exosomes promote heart repair after myocardial infarction[J]. <i>Circulation<\/i>, 2024, 150(9): 710\u2013723.","DOI":"10.1161\/CIRCULATIONAHA.123.065005"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.36","doi-asserted-by":"publisher","unstructured":"Wang S, Ma F, Huang L, et al. Dl-3-n-Butylphthalide (NBP): A promising therapeutic agent for ischemic stroke[J]. <i>CNS Neurol Disord-Drug Targets<\/i>, 2018, 17(5): 338\u2013347.","DOI":"10.2174\/1871527317666180612125843"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.37","doi-asserted-by":"publisher","unstructured":"Xiao H, Yang S, Han W, et al. Research progress on pharmacology of butylphthalide and its derivatives[J]. <i>China J Chin Mater Med (in Chinese)<\/i>, 2022, 47(13): 3425\u20133431.","DOI":"10.1016\/s1875-5364(26)61073-4"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.38","doi-asserted-by":"publisher","unstructured":"Zhu T, Dong S, Qin N, et al. Dl-3-n-butylphthalide attenuates cerebral ischemia\/reperfusion injury in mice through AMPK-mediated mitochondrial fusion[J]. <i>Front Pharmacol<\/i>, 2024, 15: 1357953.","DOI":"10.3389\/fphar.2024.1357953"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.39","doi-asserted-by":"publisher","unstructured":"Mathias K, Machado RS, Petronilho T, et al. Glial and blood-brain barrier cell-derived exosomes: Implications in stroke[J]. <i>Microvasc Res<\/i>, 2025, 160: 104812.","DOI":"10.1016\/j.mvr.2025.104812"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.40","doi-asserted-by":"publisher","unstructured":"Kalluri R, LeBleu VS. The biology, function, and biomedical applications of exosomes[J]. <i>Science<\/i>, 2020, 367(6478): eaau6977.","DOI":"10.1126\/science.aau6977"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.41","doi-asserted-by":"publisher","unstructured":"Xiao R, Wang Q, Peng J, et al. BMSCs-derived exosomal Egr2 inhibited OGD\/R-induced neuronal cell injury through the RNF8\/DAPK1 axis in ischemic stroke[J]. <i>Exp Brain Res<\/i>, 2025, 243(7): 181.","DOI":"10.1007\/s00221-025-07127-3"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.42","doi-asserted-by":"publisher","unstructured":"Cao G, Jiang N, Hu Y, et al. Ruscogenin attenuates cerebral ischemia-induced blood-brain barrier dysfunction by suppressing TXNIP\/NLRP3 inflammasome activation and the MAPK pathway[J]. <i>Int J Mol Sci<\/i>, 2016, 17(9): 1418.","DOI":"10.3390\/ijms17091418"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.43","doi-asserted-by":"publisher","unstructured":"Gao L, Dong Q, Song Z, et al. NLRP3 inflammasome: A promising target in ischemic stroke[J]. <i>Inflamm Res<\/i>, 2017, 66(1): 17\u201324.","DOI":"10.1007\/s00011-016-0981-7"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.44","doi-asserted-by":"publisher","unstructured":"Xu X, Zhang L, Ye X, et al. Nrf2\/ARE pathway inhibits ROS-induced NLRP3 inflammasome activation in BV2 cells after cerebral ischemia reperfusion[J]. <i>Inflamm Res<\/i>, 2018, 67(1): 57\u201365.","DOI":"10.1007\/s00011-017-1095-6"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.45","doi-asserted-by":"publisher","unstructured":"Liu W, Wang S, Zhang X, et al. Enhanced cardiomyocyte NLRP3 inflammasome-mediated pyroptosis promotes <sub>D<\/sub>-galactose-induced cardiac aging[J]. <i>J Am Heart Assoc<\/i>, 2024, 13(14): e032904.","DOI":"10.1161\/JAHA.123.032904"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.46","doi-asserted-by":"publisher","unstructured":"Luo X, Bao X, Weng X, et al. The protective effect of quercetin on macrophage pyroptosis <i>via<\/i> TLR2\/Myd88\/NF-\u03baB and ROS\/AMPK pathway[J]. <i>Life Sci<\/i>, 2022, 291: 120064.","DOI":"10.1016\/j.lfs.2021.120064"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.47","doi-asserted-by":"publisher","unstructured":"Zhang J, Huang L, Shi X, et al. Metformin protects against myocardial ischemia-reperfusion injury and cell pyroptosis <i>via<\/i> AMPK\/NLRP3 inflammasome pathway[J]. <i>Aging (Albany NY)<\/i>, 2020, 12(23): 24270\u201324287.","DOI":"10.18632\/aging.202143"},{"key":"10.7555\/JBR.39.20250189.swyxyjzzywb.48","doi-asserted-by":"publisher","unstructured":"Wang Z, Yang Y, Wang N, et al. RIP3 orchestrates oxidative stress and pyroptosis in doxorubicin-induced cardiotoxicity through regulation of AKT\/Nrf2 signaling cascade[J]. <i>Mol Cell Biochem<\/i>, 2025, 480(4): 2331\u20132343.","DOI":"10.1007\/s11010-024-05029-6"}],"container-title":["Journal of Biomedical Research"],"original-title":[],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T07:22:10Z","timestamp":1790061730000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.jbr-pub.org.cn\/article\/doi\/10.7555\/JBR.39.20250189"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":48,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026]]}},"alternative-id":["JBR-2025-0189"],"URL":"https:\/\/doi.org\/10.7555\/jbr.39.20250189","relation":{},"ISSN":["1674-8301","1674-8301"],"issn-type":[{"value":"1674-8301","type":"print"},{"value":"1674-8301","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]}}}