{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:54:53Z","timestamp":1790646893279,"version":"4.1.0"},"reference-count":46,"publisher":"Journal of Biomedical Research","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Biomed Res"],"published-print":{"date-parts":[[2026]]},"DOI":"10.7555\/jbr.39.20250300","type":"journal-article","created":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T12:28:09Z","timestamp":1763555289000},"page":"505","source":"Crossref","is-referenced-by-count":0,"title":["Generation of &lt;i&gt;ST6GAL1\/ST3GAL4&lt;\/i&gt; double-knockout Landrace pigs with reduced respiratory sialylated glycans and decreased influenza A virus susceptibility of derived fibroblasts &lt;i&gt;in vitro&lt;\/i&gt;"],"prefix":"10.7555","volume":"40","author":[{"given":"Lin","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyi","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxue","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhan","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinyuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianghao","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yilin","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Rao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyuan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4380","reference":[{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.1","unstructured":"World Health Organization. Influenza (seasonal)[EB\/OL]. (2025-02-28) [2025-11-12]. https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/influenza-(seasonal)."},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.2","doi-asserted-by":"publisher","unstructured":"Everest H, Hill SC, Daines R, et al. The evolution, spread and global threat of H6Nx avian influenza viruses[J]. <i>Viruses<\/i>, 2020, 12(6): 673.","DOI":"10.3390\/v12060673"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.3","doi-asserted-by":"publisher","unstructured":"Petrova VN, Russell CA. Erratum: The evolution of seasonal influenza viruses[J]. <i>Nat Rev Microbiol<\/i>, 2018, 16(1): 60.","DOI":"10.1038\/nrmicro.2017.146"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.4","doi-asserted-by":"crossref","unstructured":"B\u00f6ttcher-Friebertsh\u00e4user E, Garten W, Matrosovich M, et al. The hemagglutinin: A determinant of pathogenicity[M]\/\/Compans RW, Oldstone MBA. Influenza Pathogenesis and Control\u2014Volume \u2160. Cham: Springer, 2014: 3\u201334.","DOI":"10.1007\/82_2014_384"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.5","doi-asserted-by":"publisher","unstructured":"Park JE, Ryu Y. Transmissibility and severity of influenza virus by subtype[J]. <i>Infect Genet Evol<\/i>, 2018, 65: 288\u2013292.","DOI":"10.1016\/j.meegid.2018.08.007"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.6","doi-asserted-by":"publisher","unstructured":"Lee CY. Exploring potential intermediates in the cross-species transmission of influenza A virus to humans[J]. <i>Viruses<\/i>, 2024, 16(7): 1129.","DOI":"10.3390\/v16071129"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.7","doi-asserted-by":"publisher","unstructured":"Kristensen C, Larsen LE, Trebbien R, et al. The avian influenza A virus receptor SA-\u03b12,3-Gal is expressed in the porcine nasal mucosa sustaining the pig as a mixing vessel for new influenza viruses[J]. <i>Virus Res<\/i>, 2024, 340: 199304.","DOI":"10.1016\/j.virusres.2023.199304"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.8","doi-asserted-by":"publisher","unstructured":"Arruda B, Baker ALV, Buckley A, et al. Divergent pathogenesis and transmission of highly pathogenic avian influenza A(H5N1) in swine[J]. <i>Emerg Infect Dis<\/i>, 2024, 30(4): 738\u2013751.","DOI":"10.3201\/eid3004.231141"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.9","doi-asserted-by":"publisher","unstructured":"Trombetta C, Piccirella S, Perini D, et al. Emerging influenza strains in the last two decades: A threat of a new pandemic?[J]. <i>Vaccines (Basel)<\/i>, 2015, 3(1): 172\u2013185.","DOI":"10.3390\/vaccines3010172"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.10","doi-asserted-by":"publisher","unstructured":"\u015awierczy\u0144ska M, Mirowska-Guzel DM, Pindelska E. Antiviral drugs in influenza[J]. <i>Int J Environ Res Public Health<\/i>, 2022, 19(5): 3018.","DOI":"10.3390\/ijerph19053018"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.11","doi-asserted-by":"publisher","unstructured":"Tandel K, Sharma S, Dash PK, et al. Oseltamivir-resistant influenza A(H1N1)pdm09 virus associated with high case fatality, India 2015[J]. <i>J Med Virol<\/i>, 2018, 90(5): 836\u2013843.","DOI":"10.1002\/jmv.25013"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.12","doi-asserted-by":"publisher","unstructured":"Abed Y, Saim-Mamoun A, Boivin G. Fitness of influenza A and B viruses with reduced susceptibility to baloxavir: A mini-review[J]. <i>Rev Med Virol<\/i>, 2021, 31(3): e2175.","DOI":"10.1002\/rmv.2175"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.13","doi-asserted-by":"publisher","unstructured":"Hussain M, Galvin HD, Haw TY, et al. Drug resistance in influenza A virus: The epidemiology and management[J]. <i>Infect Drug Resist<\/i>, 2017, 10: 121\u2013134.","DOI":"10.2147\/IDR.S105473"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.14","doi-asserted-by":"publisher","unstructured":"Lee N, Hurt AC. Neuraminidase inhibitor resistance in influenza: A clinical perspective[J]. <i>Curr Opin Infect Dis<\/i>, 2018, 31(6): 520\u2013526.","DOI":"10.1097\/QCO.0000000000000498"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.15","unstructured":"World Health Organization. Recommended composition of influenza virus vaccines for use in the 2025-2026 northern hemisphere influenza season[EB\/OL]. (2025-02-28) [2025-11-12]. https:\/\/www.who.int\/publications\/m\/item\/recommended-composition-of-influenza-virus-vaccines-for-use-in-the-2025-2026-nh-influenza-season."},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.16","doi-asserted-by":"publisher","unstructured":"Harrington WN, Kackos CM, Webby RJ. The evolution and future of influenza pandemic preparedness[J]. <i>Exp Mol Med<\/i>, 2021, 53(5): 737\u2013749.","DOI":"10.1038\/s12276-021-00603-0"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.17","doi-asserted-by":"publisher","unstructured":"Houser K, Subbarao K. Influenza vaccines: Challenges and solutions[J]. <i>Cell Host Microbe<\/i>, 2015, 17(3): 295\u2013300.","DOI":"10.1016\/j.chom.2015.02.012"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.18","doi-asserted-by":"publisher","unstructured":"S\u00f6llner JH, Mettenleiter TC, Petersen B. Genome editing strategies to protect livestock from viral infections[J]. <i>Viruses<\/i>, 2021, 13(10): 1996.","DOI":"10.3390\/v13101996"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.19","doi-asserted-by":"publisher","unstructured":"Alexander DJ, Brown IH. Recent zoonoses caused by influenza A viruses[J]. <i>Rev Sci Tech<\/i>, 2000, 19(1): 197\u2013225.","DOI":"10.20506\/rst.19.1.1220"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.20","doi-asserted-by":"publisher","unstructured":"Gao F, Li P, Yin Y, et al. Molecular breeding of livestock for disease resistance[J]. <i>Virology<\/i>, 2023, 587: 109862.","DOI":"10.1016\/j.virol.2023.109862"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.21","doi-asserted-by":"publisher","unstructured":"Adli M. The CRISPR tool kit for genome editing and beyond[J]. <i>Nat Commun<\/i>, 2018, 9(1): 1911.","DOI":"10.1038\/s41467-018-04252-2"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.22","doi-asserted-by":"publisher","unstructured":"Qian L, Tang M, Yang J, et al. Targeted mutations in <i>myostatin<\/i> by zinc-finger nucleases result in double-muscled phenotype in Meishan pigs[J]. <i>Sci Rep<\/i>, 2015, 5: 14435.","DOI":"10.1038\/srep14435"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.23","doi-asserted-by":"publisher","unstructured":"Ruan J, Xu J, Chen-Tsai RY, et al. Genome editing in livestock: Are we ready for a revolution in animal breeding industry?[J]. <i>Transgenic Res<\/i>, 2017, 26(6): 715\u2013726.","DOI":"10.1007\/s11248-017-0049-7"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.24","unstructured":"Food and Agriculture Organization of the United Nations. Gene editing and food safety: Technical considerations and potential relevance to the work of Codex Alimentarius[EB\/OL]. (2023-04-17) [2025-11-12]. https:\/\/openknowledge.fao.org\/server\/api\/core\/bitstreams\/98af0834-aa7b-45ab-9263-65ae0116a0ce\/content."},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.25","doi-asserted-by":"publisher","unstructured":"Hallerman E, Bredlau J, Camargo LSA, et al. Enabling regulatory policy globally will promote realization of the potential of animal biotechnology[J]. <i>CABI Agric Biosci<\/i>, 2024, 5: 25.","DOI":"10.1186\/s43170-024-00221-6"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.26","doi-asserted-by":"publisher","unstructured":"Whitworth KM, Benne JA, Spate LD, et al. Zygote injection of CRISPR\/Cas9 RNA successfully modifies the target gene without delaying blastocyst development or altering the sex ratio in pigs[J]. <i>Transgenic Res<\/i>, 2017, 26(1): 97\u2013107.","DOI":"10.1007\/s11248-016-9989-6"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.27","doi-asserted-by":"publisher","unstructured":"Kwon T, Artiaga BL, McDowell CD, et al. Gene editing of pigs to control influenza A virus infections[J]. <i>Emerg Microbes Infect<\/i>, 2024, 13(1): 2387449.","DOI":"10.1080\/22221751.2024.2387449"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.28","doi-asserted-by":"publisher","unstructured":"M\u00fcller M, Brenig B, Winnacker EL, et al. Transgenic pigs carrying cDNA copies encoding the murine Mx1 protein which confers resistance to influenza virus infection[J]. <i>Gene<\/i>, 1992, 121(2): 263\u2013270.","DOI":"10.1016\/0378-1119(92)90130-H"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.29","doi-asserted-by":"publisher","unstructured":"Yan Q, Yang H, Yang D, et al. Production of transgenic pigs over-expressing the antiviral gene Mx1[J]. <i>Cell Regen<\/i>, 2014, 3(1): 11.","DOI":"10.1186\/2045-9769-3-11"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.30","doi-asserted-by":"publisher","unstructured":"European Food Safety Authority, European Centre for Disease Prevention and Control, European Union Reference Laboratory for Avian Influenza, et al. Avian influenza overview December 2022\u2013March 2023[J]. <i>EFSA J<\/i>, 2023, 21(3): e07917.","DOI":"10.2903\/j.efsa.2023.7917"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.31","doi-asserted-by":"publisher","unstructured":"Wu D, Huang W, Wang Y, et al. Gene silencing of \u03b2-galactosamide \u03b1-2,6-sialyltransferase 1 inhibits human influenza virus infection of airway epithelial cells[J]. <i>BMC Microbiol<\/i>, 2014, 14: 78.","DOI":"10.1186\/1471-2180-14-78"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.32","doi-asserted-by":"publisher","unstructured":"Zhao Y, Zou J, Gao Q, et al. <i>CMAS<\/i> and <i>ST3GAL4<\/i> play an important role in the adsorption of influenza virus by affecting the synthesis of sialic acid receptors[J]. <i>Int J Mol Sci<\/i>, 2021, 22(11): 6081.","DOI":"10.3390\/ijms22116081"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.33","doi-asserted-by":"publisher","unstructured":"Trebbien R, Larsen LE, Viuff BM. Distribution of sialic acid receptors and influenza A virus of avian and swine origin in experimentally infected pigs[J]. <i>Virol J<\/i>, 2011, 8: 434.","DOI":"10.1186\/1743-422X-8-434"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.34","doi-asserted-by":"publisher","unstructured":"Nelli RK, Kuchipudi SV, White GA, et al. Comparative distribution of human and avian type sialic acid influenza receptors in the pig[J]. <i>BMC Vet Res<\/i>, 2010, 6: 4.","DOI":"10.1186\/1746-6148-6-4"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.35","doi-asserted-by":"publisher","unstructured":"Costa T, Chaves AJ, Valle R, et al. Distribution patterns of influenza virus receptors and viral attachment patterns in the respiratory and intestinal tracts of seven avian species[J]. <i>Vet Res<\/i>, 2012, 43(1): 28.","DOI":"10.1186\/1297-9716-43-28"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.36","doi-asserted-by":"publisher","unstructured":"Kimble B, Nieto GR, Perez DR. Characterization of influenza virus sialic acid receptors in minor poultry species[J]. <i>Virol J<\/i>, 2010, 7: 365.","DOI":"10.1186\/1743-422X-7-365"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.37","doi-asserted-by":"publisher","unstructured":"Byrd-Leotis L, Jia N, Dutta S, et al. Influenza binds phosphorylated glycans from human lung[J]. <i>Sci Adv<\/i>, 2019, 5(2): eaav2554.","DOI":"10.1126\/sciadv.aav2554"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.38","doi-asserted-by":"publisher","unstructured":"Chung C, Yin B, Wang Q, et al. Assessment of the coordinated role of ST3GAL3, ST3GAL4 and ST3GAL6 on the \u03b12, 3 sialylation linkage of mammalian glycoproteins[J]. <i>Biochem Biophys Res Commun<\/i>, 2015, 463(3): 211\u2013215.","DOI":"10.1016\/j.bbrc.2015.05.023"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.39","doi-asserted-by":"publisher","unstructured":"Ito T, Suzuki Y, Suzuki T, et al. Recognition of <i>N<\/i>-glycolylneuraminic acid linked to galactose by the \u03b12,3 linkage is associated with intestinal replication of influenza A virus in ducks[J]. <i>J Virol<\/i>, 2000, 74(19): 9300\u20139305.","DOI":"10.1128\/JVI.74.19.9300-9305.2000"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.40","doi-asserted-by":"publisher","unstructured":"Couceiro JNSS, Paulson JC, Baum LG. Influenza virus strains selectively recognize sialyloligosaccharides on human respiratory epithelium; the role of the host cell in selection of hemagglutinin receptor specificity[J]. <i>Virus Res<\/i>, 1993, 29(2): 155\u2013165.","DOI":"10.1016\/0168-1702(93)90056-S"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.41","doi-asserted-by":"publisher","unstructured":"Monteerarat Y, Suptawiwat O, Boonarkart C, et al. Inhibition of H5N1 highly pathogenic influenza virus by suppressing a specific sialyltransferase[J]. <i>Arch Virol<\/i>, 2010, 155(6): 889\u2013893.","DOI":"10.1007\/s00705-010-0658-4"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.42","doi-asserted-by":"publisher","unstructured":"Byrd-Leotis L, Liu R, Bradley KC, et al. Shotgun glycomics of pig lung identifies natural endogenous receptors for influenza viruses[J]. <i>Proc Natl Acad Sci U S A<\/i>, 2014, 111(22): E2241\u2013E2250.","DOI":"10.1073\/pnas.1323162111"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.43","doi-asserted-by":"publisher","unstructured":"Ni Z, Wang J, Yu X, et al. Influenza virus uses mGluR2 as an endocytic receptor to enter cells[J]. <i>Nat Microbiol<\/i>, 2024, 9(7): 1764\u20131777.","DOI":"10.1038\/s41564-024-01713-x"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.44","doi-asserted-by":"publisher","unstructured":"Hedlund M, Ng E, Varki A, et al. \u03b1 2-6-Linked sialic acids on N-glycans modulate carcinoma differentiation <i>in vivo<\/i>[J]. <i>Cancer Res<\/i>, 2008, 68(2): 388\u2013394.","DOI":"10.1158\/0008-5472.CAN-07-1340"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.45","doi-asserted-by":"publisher","unstructured":"Cazet A, Julien S, Bobowski M, et al. Tumour-associated carbohydrate antigens in breast cancer[J]. <i>Breast Cancer Res<\/i>, 2010, 12(3): 204.","DOI":"10.1186\/bcr2577"},{"key":"10.7555\/JBR.39.20250300.swyxyjzzywb.46","doi-asserted-by":"publisher","unstructured":"Ednie AR, Horton KK, Wu J, et al. Expression of the sialyltransferase, ST3Gal4, impacts cardiac voltage-gated sodium channel activity, refractory period and ventricular conduction[J]. <i>J Mol Cell Cardiol<\/i>, 2013, 59: 117\u2013127.","DOI":"10.1016\/j.yjmcc.2013.02.013"}],"container-title":["Journal of Biomedical Research"],"original-title":[],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:56:12Z","timestamp":1790643372000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.jbr-pub.org.cn\/article\/doi\/10.7555\/JBR.39.20250300"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":46,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026]]}},"alternative-id":["JBR-2025-0300"],"URL":"https:\/\/doi.org\/10.7555\/jbr.39.20250300","relation":{},"ISSN":["1674-8301","1674-8301"],"issn-type":[{"value":"1674-8301","type":"print"},{"value":"1674-8301","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]}}}