{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T10:32:25Z","timestamp":1793442745008,"version":"4.5.0"},"reference-count":37,"publisher":"Wildlife Disease Association","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Wildlife Diseases"],"published-print":{"date-parts":[[2021,1,6]]},"abstract":"<jats:p>Dictyocaulus spp. infections are common in North American cervids, with Dictyocaulus viviparus described as most common. A Rocky Mountain elk (Cervus canadensis nelsoni) was found dead in Wyoming, US with significant bronchitis and pneumonia. In the bronchi and trachea, numerous large nematodes were found and grossly identified as Dictyocaulus spp. lungworms. Macroscopic alterations, such as distended interlobular septa and edema with foam and mucus observed on cut surface and in trachea and bronchi, were consistent with those commonly described in D. viviparus infections. Female lungworms were identified to Dictyocaulus spp. level via morphologic examination and molecular analyses based on mitochondrial cyclooxygenase 1 and 18S ribosomal RNA genes. A phylogenetic analysis was conducted employing the maximum likelihood method. Based on both morphologic and genetic assays, the isolated lungworms were most likely a strain of Dictyocaulus cervi. Within the female adult worms, free first stage larvae were observed besides worm eggs, which had not been described for Dictyocaulus spp. Phylogenetic analysis revealed that our parasites clustered closely with D. cervi, forming a subclade with that species within a larger clade that includes Dictyocaulus eckerti. While the elk tested positive for chronic wasting disease, it is assumed that significant pathology in the present case was caused directly by infection with the D. cervi-like lungworm, not previously described in North America.<\/jats:p>","DOI":"10.7589\/jwd-d-20-00023","type":"journal-article","created":{"date-parts":[[2020,10,19]],"date-time":"2020-10-19T16:13:06Z","timestamp":1603123986000},"page":"71-81","source":"Crossref","is-referenced-by-count":6,"title":["DICTYOCAULUS CERVI-LIKE LUNGWORM INFECTION IN A ROCKY MOUNTAIN ELK (CERVUS CANADENSIS NELSONI) FROM WYOMING, USA"],"prefix":"10.7589","volume":"57","author":[{"given":"Berit","family":"Bangoura","sequence":"first","affiliation":[{"name":"Wyoming State Veterinary Laboratory, Department of Veterinary Sciences, University of Wyoming,1174 Snowy Range Road, Laramie, Wyoming 82070, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bill","family":"Brinegar","sequence":"additional","affiliation":[{"name":"Wyoming Game and Fish Department, 1212 S Adams Street, Laramie, Wyoming 82070, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terry E.","family":"Creekmore","sequence":"additional","affiliation":[{"name":"Wyoming Game and Fish, Wyoming State Veterinary Laboratory, 1174 Snowy Range Road, Laramie, Wyoming 82070, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4415","reference":[{"key":"37","doi-asserted-by":"crossref","unstructured":"ES. Williams,  \"Vet Pathol.\"  42, \"Chronic wasting disease.\" 530 (2005)","DOI":"10.1354\/vp.42-5-530"},{"key":"21","doi-asserted-by":"publisher","unstructured":"S Kumar, G Stecher, M Li, et al.  \"Mol Biol Evol.\"  35, \"MEGA X: Molecular evolutionary genetics analysis across computing platforms.\" 1547 (2018)","DOI":"10.1093\/molbev\/msy096"},{"key":"1","doi-asserted-by":"crossref","unstructured":"Z \u00c1cs, A Hayward, L Sug\u00e1r,  \"Parasitol Res.\"  115, \"Genetic diversity and population genetics of large lungworms (Dictyocaulus, Nematoda) in wild deer in Hungary.\" 3295 (2016)","DOI":"10.1007\/s00436-016-5088-0"},{"key":"2","doi-asserted-by":"crossref","unstructured":"RE Barrett  and DE Worley  \"Bull Wildl Dis Assoc.\"  2, \"The incidence of Dictyocaulus sp. in three populations of elk in south-central Montana.\" 5 (1966)","DOI":"10.7589\/0090-3558-2.1.5"},{"key":"3","doi-asserted-by":"crossref","unstructured":"KM Bates, LP Hansen, SP Green, et al.  \"J Wildl Dis.\"  36, \"Potential for cross-transmission of Dictyocaulus viviparus between cattle and white-tailed deer.\" 774 (2000)","DOI":"10.7589\/0090-3558-36.4.774"},{"key":"4","doi-asserted-by":"crossref","unstructured":"SL Benestad  and GC Telling  \"Handb Clin Neurol.\"  153, \"Chronic wasting disease: An evolving prion disease of cervids.\" 135 (2018)","DOI":"10.1016\/B978-0-444-63945-5.00008-8"},{"key":"5","doi-asserted-by":"crossref","unstructured":"RC. Bergstrom,  \"J Wildl Dis.\"  11, \"Prevalence of Dictyocaulus viviparous infection in Rocky Mountain elk in Teton County, Wyoming.\" 40 (1975)","DOI":"10.7589\/0090-3558-11.1.40"},{"key":"6","unstructured":"S Bienioschek, S Rehbein, R Ribbeck,  \"Appl Parasitol.\"  37, \"Cross-infections between fallow deer and domestic ruminants with large lungworms (Dictyocaulus spp.).\" 229 (1996)"},{"key":"7","doi-asserted-by":"crossref","unstructured":"C Cantacessi, RB Gasser, C Strube, et al.  \"Biotechnol Adv.\"  29, \"Deep insights into Dictyocaulus viviparus transcriptomes provides unique prospects for new drug targets and disease intervention.\" 261 (2011)","DOI":"10.1016\/j.biotechadv.2010.11.005"},{"key":"8","doi-asserted-by":"crossref","unstructured":"RA Carreno, N Diez-Ba\u00f1os, MR Del Hidalgo-Arg\u00fcello, et al.  \"J Parasitol.\"  95, \"Characterization of Dictyocaulus species (Nematoda: Trichostrongyloidea) from three species of wild ruminants in northwestern Spain.\" 966 (2009)","DOI":"10.1645\/GE-1791.1"},{"key":"9","unstructured":"EA. Chapin,  \"J Agric Res.\"  30, \"New nematodes from North American mammals.\" 667 (1925)"},{"key":"10","unstructured":"G. Dikmans,  \"J Wash Acad Sci.\"  26, \"A note on Dictyocaulus from domestic and wild ruminants.\" 298 (1936)"},{"key":"11","doi-asserted-by":"crossref","unstructured":"BP Divina  and J H\u00f6glund  \"J Helminthol.\"  76, \"Heterologous transmission with Dictyocaulus capreolus from roe deer (Capreolus capreolus) to cattle (Bos taurus).\" 125 (2002)","DOI":"10.1079\/JOH2001100"},{"key":"12","doi-asserted-by":"crossref","unstructured":"BP Divina, E Wilhelmsson, JG Mattsson, et al.  \"Parasitology.\"  121, \"Identification of Dictyocaulus spp. in ruminants by morphological and molecular analyses.\" 193 (2000)","DOI":"10.1017\/S0031182099006162"},{"key":"13","doi-asserted-by":"crossref","unstructured":"BP Divina, E Wilhelmsson, T M\u00f6rner, et al.  \"J Wildl Dis.\"  38, \"Molecular identification and prevalence of Dictyocaulus spp. (Trichostrongyloidea: Dictyocaulidae) in Swedish semi-domestic and free-living cervids.\" 769 (2002)","DOI":"10.7589\/0090-3558-38.4.769"},{"key":"14","doi-asserted-by":"crossref","unstructured":"MC Durette-Desset, L Hugonnet, AG Chabaud,  \"Ann Parasitol Hum Comp.\"  63, \"Redescription of Dictyocaulus noerneri Ralliet et Henry, 1907, parasite of Capreolus capreolus in Europe. Comparison with D. viviparus (Bloch, 1782), a parasite of cattle.\" 285 (1988)","DOI":"10.1051\/parasite\/1988634285"},{"key":"15","doi-asserted-by":"crossref","unstructured":"WJ Foreyt, D Hunter, JG Cook, et al.  \"J Wildl Dis.\"  36, \"Susceptibility of elk to lungworms from cattle.\" 729 (2000)","DOI":"10.7589\/0090-3558-36.4.729"},{"key":"16","doi-asserted-by":"crossref","unstructured":"LM Gibbons  and J H\u00f6glund  \"J Helminthol.\"  76, \"Dictyocaulus capreolus n. sp. (Nematoda: Trichostrongyloidea) from roe deer, Capreolus capreolus and moose, Alces alces in Sweden.\" 119 (2002)","DOI":"10.1079\/JOH2001108"},{"key":"17","doi-asserted-by":"crossref","unstructured":"S Gilch, N Chitoor, Y Taguchi, et al.  \"Prion proteins.\"  , \"Chronic wasting disease.\" 51 (2011)","DOI":"10.1007\/128_2011_159"},{"key":"18","doi-asserted-by":"crossref","unstructured":"J H\u00f6glund, A Engstr\u00f6m, DA Morrison, et al.  \"Parasitol Res.\"  103, \"Limited sequence variation in the major sperm protein 1 (MSP) gene within populations and species of the genus Dictyocaulus (Nematoda).\" 11 (2008)","DOI":"10.1007\/s00436-008-0877-8"},{"key":"19","unstructured":"J Jansen  and FH Borgsteede  \"Tijdschr Diergeneeskd.\"  115, \"Dictyocaulus species, lungworms in cattle and deer in The Netherlands.\" 155 (1990)"},{"key":"20","doi-asserted-by":"crossref","unstructured":"M. Kimura,  \"J Mol Evol.\"  16, \"A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences.\" 111 (1980)","DOI":"10.1007\/BF01731581"},{"key":"22","doi-asserted-by":"crossref","unstructured":"CG Mackintosh, C Cowie, K Fraser, et al.  \"Vet Parasitol.\"  199, \"Reduced efficacy of moxidectin and abamectin in young red deer (Cervus elaphus) after 20 years of moxidectin pour-on use on a New Zealand deer farm.\" 81 (2014)","DOI":"10.1016\/j.vetpar.2013.09.028"},{"key":"23","unstructured":"et al.  \"Basic local alignment search tool (BLAST).\"  ,  (2020)"},{"key":"24","doi-asserted-by":"crossref","unstructured":"P Oyarz\u00fan-Ruiz, P Mu\u00f1oz, G Valenzuela,  \"Rev Mex Biodiversity.\"  89, \"Southern pudu (Pudu puda) (Artiodactyla: Cervidae) as an additional host for Dictyocaulus eckerti (Strongylida: Dictyocaulidae).\" 301 (2018)","DOI":"10.22201\/ib.20078706e.2018.1.2313"},{"key":"25","doi-asserted-by":"crossref","unstructured":"R Panadero, EB Carrillo, C L\u00f3pez, et al.  \"Vet Parasitol.\"  99, \"Bronchopulmonary helminths of roe deer (Capreolus capreolus) in the northwest of Spain.\" 221 (2001)","DOI":"10.1016\/S0304-4017(01)00465-4"},{"key":"26","doi-asserted-by":"crossref","unstructured":"C. Panuska,  \"Vet Clin North Am Food Anim Pract.\"  22, \"Lungworms of ruminants.\" 583 (2006)","DOI":"10.1016\/j.cvfa.2006.06.002"},{"key":"27","doi-asserted-by":"crossref","unstructured":"JC Patterson-Kane, LM Gibbons, R Jefferies, et al.  \"J Vet Diagn Invest.\"  21, \"Pneumonia from Angiostrongylus vasorum infection in a red panda (Ailurus fulgens fulgens).\" 270 (2009)","DOI":"10.1177\/104063870902100219"},{"key":"28","unstructured":"T. Pojar,  \"Wildlife forensic field manual.\"  , \"Field aging of mule deer, elk, and pronghorns.\" 142 (1997)"},{"key":"29","doi-asserted-by":"crossref","unstructured":"PJ Presidente  and SE Knapp  \"J Wildl Dis.\"  9, \"Susceptibility of cattle to an isolate of Dictyocaulus viviparus from black-tailed deer.\" 41 (1973)","DOI":"10.7589\/0090-3558-9.1.41"},{"key":"30","doi-asserted-by":"crossref","unstructured":"AM. Pyziel,  \"Acta Parasitol.\"  59, \"Molecular analysis of lungworms from European bison (Bison bonasus) on the basis of small subunit ribosomal RNA gene (SSU).\" 122 (2014)","DOI":"10.2478\/s11686-014-0219-1"},{"key":"31","doi-asserted-by":"crossref","unstructured":"AM Pyziel, I Dolka, J Werszko, et al.  \"Vet Parasitol.\"  261, \"Pathological lesions in the lungs of red deer Cervus elaphus (L.) induced by a newly-described Dictyocaulus cervi (Nematoda: Trichostrongyloidea).\" 22 (2018a)","DOI":"10.1016\/j.vetpar.2018.08.003"},{"key":"32","doi-asserted-by":"crossref","unstructured":"AM Pyziel, Z Laskowski, AW Demiaszkiewicz, et al.  \"J Parasitol.\"  103, \"Interrelationships of Dictyocaulus spp. in wild ruminants with morphological description of Dictyocaulus cervi n. sp. (Nematoda: Trichostrongyloidea) from red deer, Cervus elaphus.\" 506 (2017)","DOI":"10.1645\/16-75"},{"key":"33","doi-asserted-by":"crossref","unstructured":"AM Pyziel, Z Laskowski, J H\u00f6glund,  \"Parasitol Res.\"  117, \"An assessment of the use of cox1 and cox3 mitochondrial genetic markers for the identification of Dictyocaulus spp. (Nematoda: Trichostrongyloidea) in wild ruminants.\" 2341 (2018b)","DOI":"10.1007\/s00436-018-5904-9"},{"key":"34","doi-asserted-by":"crossref","unstructured":"EC Reissig, AR Massone, B Iovanitti, et al.  \"J Wildl Dis.\"  54, \"A survey of parasite lesions in wild red deer (Cervus elaphus) from Argentina.\" 782 (2018)","DOI":"10.7589\/2017-06-136"},{"key":"35","doi-asserted-by":"crossref","unstructured":"JC Rhyan, K Aune, DR Ewalt, et al.  \"J Wildl Dis.\"  33, \"Survey of free-ranging elk from Wyoming and Montana for selected pathogens.\" 290 (1997)","DOI":"10.7589\/0090-3558-33.2.290"},{"key":"36","doi-asserted-by":"crossref","unstructured":"MS Sepulveda  and JM Kinsella  \"J Vis Exp.\"  82, \"Helminth collection and identification from wildlife.\" e51000 (2013)","DOI":"10.3791\/51000"}],"container-title":["Journal of Wildlife Diseases"],"original-title":[],"link":[{"URL":"http:\/\/www.jwildlifedis.org\/doi\/pdf\/10.7589\/JWD-D-20-00023","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T09:54:49Z","timestamp":1793440489000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/journal-of-wildlife-diseases\/volume-57\/issue-1\/JWD-D-20-00023\/DICTYOCAULUS-CERVI-LIKE-LUNGWORM-INFECTION-IN-A-ROCKY-MOUNTAIN-ELK\/10.7589\/JWD-D-20-00023.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,6]]},"references-count":37,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,1,1]]}},"URL":"https:\/\/doi.org\/10.7589\/jwd-d-20-00023","relation":{},"ISSN":["0090-3558","1943-3700"],"issn-type":[{"value":"0090-3558","type":"print"},{"value":"1943-3700","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,6]]}}}