{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T08:36:21Z","timestamp":1793435781738,"version":"4.5.0"},"reference-count":69,"publisher":"Wildlife Disease Association","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Wildlife Diseases"],"published-print":{"date-parts":[[2021,10,11]]},"abstract":"<jats:p>The common vole (Microtus arvalis) is a major agricultural pest in Europe and is a reservoir for several zoonotic agents, such as Leptospira spp. and Tula orthohantavirus (TULV). However, little is known about the occurrence of those pathogens in voles from Spain, where the species has largely expanded its distribution range in the past decades, causing agricultural pests and zoonotic diseases. For a molecular survey, 580 common voles and six Lusitanian pine voles (Microtus lusitanicus) were collected in 26 localities from four provinces of northwestern Spain. We assessed the presence of Leptospira spp. DNA in kidney tissue by PCR targeting the lipL32 gene, detecting a prevalence of 7.9% (95% confidence interval, 5.9\u201310.4) for common voles and of 33.3% (95% confidence interval, 4.3\u201377.7) for Lusitanian pine voles. We identified Leptospira kirschneri in 24 animals and Leptospira borgpetersenii in two animals, using secY gene\u2013specific PCR. We analyzed environmental and demographic factors (such as age class, weight, and sex) and population dynamics data for their potential effect on the Leptospira spp. prevalence in those voles. The Leptospira spp. DNA detection rate in common voles increased significantly with maximum air temperature, vole weight, and amount of accumulated rainfall during the 90 d before capture and within the peak phase of the population cycle. We assessed the presence of TULV in lung tissue of 389 voles by reverse-transcription PCR, with no positive results. The absence of TULV might be explained by the evolutionary isolation of the common vole in Spain. The detection of two Leptospira genomospecies underlines the necessity for further typing efforts to understand the epidemiology of leptospiral infection in the common vole and the potential risk for human health in Spain.<\/jats:p>","DOI":"10.7589\/jwd-d-20-00109","type":"journal-article","created":{"date-parts":[[2021,10,11]],"date-time":"2021-10-11T01:19:32Z","timestamp":1633915172000},"page":"733-742","source":"Crossref","is-referenced-by-count":7,"title":["FREQUENT LEPTOSPIRA SPP. DETECTION BUT ABSENCE OF TULA ORTHOHANTAVIRUS IN MICROTUS SPP. VOLES, NORTHWESTERN SPAIN"],"prefix":"10.7589","volume":"57","author":[{"given":"Kathrin","family":"Jeske","sequence":"first","affiliation":[{"name":"Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, S\u00fcdufer 10, 17493 Greifswald\u2013Insel Riems, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duygu","family":"Emirhar","sequence":"additional","affiliation":[{"name":"Department for Biological Safety, Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas T.","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n en Recursos Cineg\u00e9ticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo 12, 13071 Ciudad Real, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Gonz\u00e1lez-Barrio","sequence":"additional","affiliation":[{"name":"SALUVET, University Complutense (UCM), Av. Puerta de Hierro, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro P.","family":"Olea","sequence":"additional","affiliation":[{"name":"Departamento de Ecolog\u00eda, Universidad Aut\u00f3noma de Madrid (UAM), C\/Darwin 2, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Ruiz","family":"Fons","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n en Recursos Cineg\u00e9ticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo 12, 13071 Ciudad Real, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Schulz","sequence":"additional","affiliation":[{"name":"Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, S\u00fcdufer 10, 17493 Greifswald\u2013Insel Riems, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Mayer-Scholl","sequence":"additional","affiliation":[{"name":"Department for Biological Safety, Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald","family":"Heckel","sequence":"additional","affiliation":[{"name":"Institute of Ecology and Evolution, University of Bern, Baltzerstrasse 6, 3012 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rainer G.","family":"Ulrich","sequence":"additional","affiliation":[{"name":"Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, S\u00fcdufer 10, 17493 Greifswald\u2013Insel Riems, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4415","reference":[{"key":"2","doi-asserted-by":"crossref","unstructured":"G Andre-Fontaine, F Aviat, C Thorin,  \"Curr Microbiol.\"  71, \"Waterborne leptospirosis: Survival and preservation of the virulence of pathogenic Leptospira spp. in fresh water.\" 136 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Diseases"],"original-title":[],"deposited":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T08:28:29Z","timestamp":1793435309000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/journal-of-wildlife-diseases\/volume-57\/issue-4\/JWD-D-20-00109\/FREQUENT-LEPTOSPIRA-SPP-DETECTION-BUT-ABSENCE-OF-TULA-ORTHOHANTAVIRUS-IN\/10.7589\/JWD-D-20-00109.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,11]]},"references-count":69,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2021,10,1]]}},"URL":"https:\/\/doi.org\/10.7589\/jwd-d-20-00109","relation":{},"ISSN":["0090-3558","1943-3700"],"issn-type":[{"value":"0090-3558","type":"print"},{"value":"1943-3700","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,11]]}}}