{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T09:33:48Z","timestamp":1793439228122,"version":"4.5.0"},"reference-count":21,"publisher":"Wildlife Disease Association","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Wildlife Diseases"],"published-print":{"date-parts":[[2021,7,5]]},"abstract":"<jats:p>Borrelia burgdorferi and Borrelia miyamotoi are tickborne zoonotic pathogens in Canada. Both bacteria are vectored by ticks, Ixodes scapularis in Atlantic Canada, but require wildlife reservoir species to maintain the bacteria for retransmission to future generations of ticks. Coyotes (Canis latrans) are opportunistic feeders, resulting in frequent contact with other animals and with ticks. Because coyotes are closely related to domestic dogs (Canis lupus familiaris), it is probable that coyote susceptibility to Borrelia infection is similar to that of dogs. We collected livers and kidneys of eastern coyotes from licensed harvesters in Nova Scotia, Canada, and tested them using nested PCR for the presence of B. burgdorferi, B. miyamotoi, and Dirofilaria immitis. Blood obtained from coyote livers was also tested serologically for antibodies to B. burgdorferi, Ehrlichia canis, Anaplasma phagocytophilum, and D. immitis. Borrelia burgdorferi and D. immitis were detected by both nested PCR and serology tests. Seroreactivity to A. phagocytophilum was also found. Borrelia miyamotoi and E. canis were not detected. Our results show that coyotes in Nova Scotia have been exposed to a number of vectorborne pathogens.<\/jats:p>","DOI":"10.7589\/jwd-d-20-00188","type":"journal-article","created":{"date-parts":[[2021,7,5]],"date-time":"2021-07-05T10:01:06Z","timestamp":1625479266000},"page":"678-682","source":"Crossref","is-referenced-by-count":11,"title":["Detection of Borrelia spp., Ehrlichia canis, Anaplasma phagocytophilum, and Dirofilaria immitis in Eastern Coyotes (Canis latrans) in Nova Scotia, Canada"],"prefix":"10.7589","volume":"57","author":[{"given":"Christopher B.","family":"Zinck","sequence":"first","affiliation":[{"name":"Department of Biology, Mount Allison University, 63B York Street, Sackville, New Brunswick, E4L 1G7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenna M.","family":"Priest","sequence":"additional","affiliation":[{"name":"Department of Biology, Acadia University, 15 University Avenue, Wolfville, Nova Scotia, B4P 2R6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dave","family":"Shutler","sequence":"additional","affiliation":[{"name":"Department of Biology, Acadia University, 15 University Avenue, Wolfville, Nova Scotia, B4P 2R6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Boudreau","sequence":"additional","affiliation":[{"name":"Wildlife Division, Department of Lands and Forestry, 136 Exhibition Street, Kentville, Nova Scotia, B4N 4E5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vett K.","family":"Lloyd","sequence":"additional","affiliation":[{"name":"Department of Biology, Mount Allison University, 63B York Street, Sackville, New Brunswick, E4L 1G7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4415","reference":[{"key":"3","doi-asserted-by":"crossref","unstructured":"D Bowman, SE Little, L Lorentzen, et al.  \"Vet Parasitol.\"  160, \"Prevalence and geographic distribution of Dirofilaria immitis, Borrelia burgdorferi, Ehrlichia canis, and Anaplasma phagocytophilum in dogs in the United States: Results of a national clinic-based serologic survey.\" 138 (2009)","DOI":"10.1016\/j.vetpar.2008.10.093"},{"key":"19","doi-asserted-by":"crossref","unstructured":"BN Sacks, BB Chomel, RW Kasten, et al.  \"Vet Parasitol.\"  109, \"Validation for use with coyotes (Canis latrans) of a commercially available enzyme-linked immunosorbent assay for Dirofilaria immitis.\" 45 (2002)","DOI":"10.1016\/S0304-4017(02)00254-6"},{"key":"1","doi-asserted-by":"crossref","unstructured":"AG. 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