{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T09:33:47Z","timestamp":1793439227359,"version":"4.5.0"},"reference-count":33,"publisher":"Wildlife Disease Association","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Wildlife Diseases"],"published-print":{"date-parts":[[2021,7,5]]},"abstract":"<jats:p>Aujeszky's disease (AD, pseudorabies) eradication programs in domestic pigs are implemented in several European countries where AD virus (ADV) circulates in local wild boar (Sus scrofa), making studies on ADV infection dynamics in wild boar increasingly relevant. The objective of our study was to characterize ADV dynamics in wild boar at a site in central Portugal and compare this site to three enzootic sites in central Spain. A total of 235 wild boar were sampled during the hunting season 2014\u201315. We collected serum, tissues (oropharyngeal tonsils and trigeminal and sacral ganglia), and swabs (oral, nasal, and genital) and analyzed these samples to detect ADV antibodies (enzyme-linked immunosorbent assay) and DNA (PCR). An overall seroprevalence of 42.6% was found (range 12.7\u201357.7%), being highest in adults (54.1%; 72\/133). Overall, 2.8% (3\/108) oral, 6.4% (7\/109) nasal, and 12.8% (12\/94) genital swabs were PCR positive. We found 20.4% (20\/98) of the wild boar had at least one positive swab and were considered shedders. We found ADV in tissues of five animals; of 111 tonsils, three (2.7%) were PCR positive. Trigeminal (2\/48; 4%) and sacral (2\/53; 4%) ganglia collected in central Portugal, pertaining to three animals, were positive for ADV DNA. Logistic regression models showed that seroprevalence was influenced by site and age, whereas ADV shedding was influenced by site. Our study describes patterns of ADV infection in wild boar in Portugal and shows that wild boar also pose a risk, albeit lower than that in central Spain, for the eradication of AD from extensively managed domestic pigs in Portugal.<\/jats:p>","DOI":"10.7589\/jwd-d-20-00197","type":"journal-article","created":{"date-parts":[[2021,7,5]],"date-time":"2021-07-05T10:01:34Z","timestamp":1625479294000},"page":"543-552","source":"Crossref","is-referenced-by-count":4,"title":["AUJESZKY'S DISEASE IN HUNTED WILD BOAR (SUS SCROFA) IN THE IBERIAN PENINSULA"],"prefix":"10.7589","volume":"57","author":[{"given":"Alexandra","family":"M\u00fcller","sequence":"first","affiliation":[{"name":"Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar (ICBAS), Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natacha","family":"Melo","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Veterin\u00e1rias, Universidade de Tr\u00e1s-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Gonz\u00e1lez-Barrio","sequence":"additional","affiliation":[{"name":"SALUVET, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s\/n, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madalena Vieira","family":"Pinto","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Veterin\u00e1rias, Universidade de Tr\u00e1s-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Ruiz-Fons","sequence":"additional","affiliation":[{"name":"Health & Biotechnology (SaBio) Group, Instituto de Investigaci\u00f3n en Recursos Cineg\u00e9ticos (IREC) (CSIC-UCLM), Ronda de Toledo 12, 13071 Ciudad Real, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4415","reference":[{"key":"18","doi-asserted-by":"crossref","unstructured":"T M\u00fcller, EC Hahn, F Tottewitz, et al.  \"Arch Virol.\"  156, \"Pseudorabies virus in wild swine: A global perspective.\" 1691 (2011)","DOI":"10.1007\/s00705-011-1080-2"},{"key":"20","unstructured":"et al.  \"Aujeszky's disease (infection with Aujeszky's disease virus).\"  ,  (2018)"},{"key":"1","doi-asserted-by":"crossref","unstructured":"P Acevedo, J Vicente, U H\u00f6fle, et al.  \"Epidemiol Infect.\"  135, \"Estimation of European wild boar relative abundance and aggregation: A novel method in epidemiological risk assessment.\" 519 (2007)","DOI":"10.1017\/S0950268806007059"},{"key":"2","doi-asserted-by":"crossref","unstructured":"A Arenas-Montes, I Garc\u00eda-Bocanegra, J Paniagua, et al.  \"Eur J Wildl Res.\"  59, \"Blood sampling by puncture in the cavernous sinus from hunted wild boar.\" 299 (2013)","DOI":"10.1007\/s10344-013-0701-3"},{"key":"3","doi-asserted-by":"crossref","unstructured":"M Boadella, C Gort\u00e1zar, J Vicente, et al.  \"BMC Vet Res.\"  8, \"Wild boar: An increasing concern for Aujeszky's disease control in pigs?\" 7 (2012a)","DOI":"10.1186\/1746-6148-8-7"},{"key":"4","doi-asserted-by":"crossref","unstructured":"M Boadella, J Vicente, F Ruiz-Fons, et al.  \"Prev Vet Med.\"  107, \"Effects of culling Eurasian wild boar on the prevalence of Mycobacterium bovis and Aujeszky's disease virus.\" 214 (2012b)","DOI":"10.1016\/j.prevetmed.2012.06.001"},{"key":"5","doi-asserted-by":"crossref","unstructured":"D Cano-Terriza, R Mart\u00ednez, A Moreno, et al.  \"EcoHealth.\"  16, \"Survey of Aujeszky's disease virus in hunting dogs from Spain.\" 351 (2019)","DOI":"10.1007\/s10393-019-01426-2"},{"key":"6","unstructured":"C Caruso, N Vitale, R Prato, et al.  \"Vet Ital.\"  54, \"Pseudorabies virus in northwest Italian wild boar (Sus scrofa) populations: Prevalence and risk factors to support a territorial risk-based surveillance.\" 1006 (2018)"},{"key":"7","doi-asserted-by":"crossref","unstructured":"L Casades-Mart\u00ed, D Gonz\u00e1lez-Barrio, L Royo-Hern\u00e1ndez, et al.  \"Transbound Emerg Dis.\"  67, \"Dynamics of Aujeszky's disease virus infection in wild boar in enzootic scenarios.\" 388 (2020)","DOI":"10.1111\/tbed.13362"},{"key":"8","unstructured":"et al.  \"Off J Eur Union.\"  59, \"Commission Decision 2008\/185\/EC. 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