{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T08:32:33Z","timestamp":1793435553925,"version":"4.5.0"},"reference-count":31,"publisher":"Wildlife Disease Association","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Wildlife Diseases"],"published-print":{"date-parts":[[2022,1,4]]},"abstract":"<jats:p>Hemotropic mycoplasmas cause hemolytic anemia in a variety of wild and domestic mammals. Despite growing evidence about their widespread presence and genetic diversity in wildlife, their presence has never been investigated in Chilean artiodactyls. We aimed to describe the presence and diversity of hemoplasmas in pudus (Pudu puda), a small cervid native to Chile. Hemoplasma infection was assessed in blood samples from 43 wild and 33 captive pudus from central and southern Chile by direct sequencing of the 16S rRNA gene. We detected hemoplasmas in 13%, with no statistical differences between wild (19%) and captive animals (6%). A sequence closely related to Mycoplasma ovis was present both in wild (14%) and captive (6%) pudus. Two previously undescribed sequences, classified in a clade including hemoplasmas from carnivores, were found in one wild pudu each. This study presents the first evidence of the presence of M. ovislike organisms in Chile and of the susceptibility of pudus to infection with hemoplasmas. Further research is needed to understand the pathologic consequences of this pathogen for pudus, its effects at the population level, and their potential impact on the health small ruminants and other wildlife species in Chile.<\/jats:p>","DOI":"10.7589\/jwd-d-21-00057","type":"journal-article","created":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T06:54:10Z","timestamp":1641279250000},"page":"8-14","source":"Crossref","is-referenced-by-count":9,"title":["MOLECULAR DETECTION AND CHARACTERIZATION OF HEMOPLASMAS IN THE PUDU (PUDU PUDA), A NATIVE CERVID FROM CHILE"],"prefix":"10.7589","volume":"58","author":[{"given":"Ezequiel","family":"Hidalgo-Hermoso","sequence":"first","affiliation":[{"name":"Conservation and Research Department (CIBZ), Parque Zool\u00f3gico Buin Zoo, Panamericana Sur Km 32, Buin, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Cabello","sequence":"additional","affiliation":[{"name":"Centro de conservaci\u00f3n de la biodiversidad, Chilo\u00e9-Silvestre, Nal Bajo, Ancud, Chilo\u00e9, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Novoa-Lozano","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal (INIA-CISA), Carretera de Algete a El Casar s\/n, 28130 Valdeolmos, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebasti\u00e1n","family":"Celis","sequence":"additional","affiliation":[{"name":"Departamento de Veterinaria, Parque Zool\u00f3gico Buin Zoo, Panamericana Sur Km 32, Buin, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Ortiz","sequence":"additional","affiliation":[{"name":"Departamento de Veterinaria, Parque Zool\u00f3gico Buin Zoo, Panamericana Sur Km 32, Buin, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Kemec","sequence":"additional","affiliation":[{"name":"Departamento de Veterinaria, Parque Zool\u00f3gico Buin Zoo, Panamericana Sur Km 32, Buin, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rocio","family":"Lagos","sequence":"additional","affiliation":[{"name":"Laboratorio Cl\u00ednico, Hospital Veterinario SOS Buin Zoo, Panamericana Sur Km 32, Buin, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Verasay","sequence":"additional","affiliation":[{"name":"School of Livestock and Veterinary Sciences, University of Chile, Av. Santa Rosa, 11735 Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myra","family":"Mansell-Venegas","sequence":"additional","affiliation":[{"name":"Conservation and Research Department (CIBZ), Parque Zool\u00f3gico Buin Zoo, Panamericana Sur Km 32, Buin, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dario","family":"Moreira-Arce","sequence":"additional","affiliation":[{"name":"Departamento de Gesti\u00f3n Agraria, Universidad de Santiago de Chile, Av. Libertador Bernardo O'Higgins 3363 P.C.: 7254758, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo M.","family":"Vergara","sequence":"additional","affiliation":[{"name":"Departamento de Gesti\u00f3n Agraria, Universidad de Santiago de Chile, Av. Libertador Bernardo O'Higgins 3363 P.C.: 7254758, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Mill\u00e1n","sequence":"additional","affiliation":[{"name":"Instituto Agroalimentario de Arag\u00f3n-IA2 (Universidad de Zaragoza-CITA), Miguel Servet 177, 50013, Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Esper\u00f3n","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal (INIA-CISA), Carretera de Algete a El Casar s\/n, 28130 Valdeolmos, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4415","reference":[{"key":"5","doi-asserted-by":"crossref","unstructured":"J Cabello, L Altet, C Napolitano, et al.  \"Vet Microbiol.\"  167, \"Survey of infectious agents in the endangered Darwin's fox (Lycalopex fulvipes): High prevalence and diversity of hemotrophic mycoplasmas.\" 448 (2013)","DOI":"10.1016\/j.vetmic.2013.09.034"},{"key":"29","unstructured":"K Tamura  and M Nei  \"Mol Biol Evol.\"  10, \"Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees.\" 512 (1993)"},{"key":"12","doi-asserted-by":"publisher","unstructured":"S Kumar, G Stecher, M Li, et al.  \"Mol Biol Evol.\"  35, \"MEGA X: Molecular evolutionary genetics analysis across computing platforms.\" 1547 (2018)","DOI":"10.1093\/molbev\/msy096"},{"key":"18","doi-asserted-by":"crossref","unstructured":"J Mill\u00e1n, M L\u00f3pez-Roig, V Delicado, et al.  \"Comp Immunol Microbiol Infect Dis.\"  39, \"Widespread infection with hemotropic mycoplasmas in bats in Spain, including a hemoplasma closely related to \u201cCandidatus Mycoplasma hemohominis.\" 9 (2015)","DOI":"10.1016\/j.cimid.2015.01.002"},{"key":"22","doi-asserted-by":"crossref","unstructured":"I Sacrist\u00e1n, F Acu\u00f1a, E Aguilar, et al.  \"Sci Rep.\"  9, \"Assessing cross-species transmission of hemoplasmas at the wild-domestic felid interface in Chile using genetic and landscape variables analysis.\" 16816 (2019)","DOI":"10.1038\/s41598-019-53184-4"},{"key":"24","doi-asserted-by":"crossref","unstructured":"F Soto, R Walker, M Sep\u00falveda, et al.  \"Comp Immunol Microbiol Infect Dis.\"  50, \"Occurrence of canine hemotropic mycoplasmas in domestic dogs from urban and rural areas of the Valdivia Province, Southern Chile.\" 70 (2017)","DOI":"10.1016\/j.cimid.2016.11.013"},{"key":"20","unstructured":"\"Basic local alignment search tool.\"  (2021)"},{"key":"6","doi-asserted-by":"crossref","unstructured":"S Di Cataldo, E Hidalgo-Hermoso, I Sacrist\u00e1n, et al.  \"Appl Environ Microbiol.\"  86, \"Hemoplasmas are endemic and cause asymptomatic infection in the endangered Darwin's fox (Lycalopex fulvipes).\" e00779 (2020)","DOI":"10.1128\/AEM.00779-20"},{"key":"1","doi-asserted-by":"crossref","unstructured":"A Alab\u00ed, G Monti, C Otth, et al.  \"Microorganisms.\"  8, \"Molecular survey and genetic diversity of hemoplasmas in rodents from Chile.\" 1493 (2020)","DOI":"10.3390\/microorganisms8101493"},{"key":"2","doi-asserted-by":"crossref","unstructured":"MR Andr\u00e9, JM Barbanti Duarte, LR Gon\u00e7alves, et al.  \"Epidemiol Infect.\"  148, \"New records and genetic diversity of Mycoplasma ovis in free-ranging deer in Brazil.\" e6 (2020)","DOI":"10.1017\/S0950268819002218"},{"key":"3","unstructured":"\"Supreme decree no. 151 of the Ministerio Secretaria General de la Presidencia de Chile. 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