{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T07:32:03Z","timestamp":1793431923320,"version":"4.5.0"},"reference-count":51,"publisher":"Wildlife Disease Association","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Wildlife Diseases"],"published-print":{"date-parts":[[2022,7,25]]},"abstract":"<jats:p>Research on host response to infectious disease often involves pharmacological induction of immunosuppression, frequently through administration of dexamethasone. Reports on the effect of dexamethasone in birds are largely restricted to poultry and pigeons. This study describes changes in white blood cell (WBC) differentials, hemoparasite counts, splenic histology, and splenic CD3 immunoreactivity in House Finches (Haemorhous mexicanus). Experimental group birds (n=9) were treated with a daily intramuscular injection of 25 \u00b5g of dexamethasone for 8 d; a control group (n=9) received daily saline solution. Smears were made with blood collected immediately before the first dose (day 0) and on d 4, 8, and 9, and stained with modified Wright. The WBC differential counts were performed by three blinded observers, parasite counts by two blinded observers, and histology by one blinded observer. Dexamethasone-treated birds experienced relative heterophilia and lymphopenia on d 4 (P=0.008); heterophilia was also present at d 8 (P=0.018). Hemosporidian counts were significantly increased in dexamethasone-treated birds on d 4 and 8 (P=0.048 and P=0.031, respectively). In contrast with control birds, all dexamethasone-treated birds lacked histologically apparent splenic lymphoid follicles (P&lt;0.001). No significant difference was observed in splenic CD3 immunoreactivity between groups. Our results indicate that dexamethasone has an effect on the hematologic profile of House Finches and suggest that it may be a useful method to induce immunosuppression in this species.<\/jats:p>","DOI":"10.7589\/jwd-d-21-00129","type":"journal-article","created":{"date-parts":[[2022,7,25]],"date-time":"2022-07-25T06:59:21Z","timestamp":1658732361000},"page":"512-523","source":"Crossref","is-referenced-by-count":3,"title":["THE EFFECT OF DEXAMETHASONE ON HEMATOLOGIC PROFILES, HEMOSPORIDIAN INFECTION, AND SPLENIC HISTOLOGY IN HOUSE FINCHES (HAEMORHOUS MEXICANUS)"],"prefix":"10.7589","volume":"58","author":[{"given":"Esther E. V.","family":"Crouch","sequence":"first","affiliation":[{"name":"Charles River Laboratories, Pathology, 251 Ballardvale Street, Wilmington, Massachusetts 01887, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Teresa","family":"Reinoso-Perez","sequence":"additional","affiliation":[{"name":"Cornell Laboratory of Ornithology, Cornell University, 159 Sapsucker Woods Road, Ithaca, New York 14850, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raphael V.","family":"Vanderstichel","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, College of Veterinary Medicine, 240 Farrier Road, Long Island University, 720 Northern Boulevard, Brookville, New York 11548, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keila V.","family":"Dhondt","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Cornell University, 240 Farrier Road, Ithaca, New York 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9 A.","family":"Dhondt","sequence":"additional","affiliation":[{"name":"Cornell Laboratory of Ornithology, Cornell University, 159 Sapsucker Woods Road, Ithaca, New York 14850, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Daniel","family":"Cruz Otero","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Department of Biomedical Sciences, 240 Farrier Road, Cornell University, Ithaca, New York 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tara","family":"Piech","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biomedical Sciences, College of Veterinary Medicine, Long Island University, 720 Northern Boulevard, Brookville, New York 11548, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda J.","family":"Forz\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biomedical Sciences, College of Veterinary Medicine, Long Island University, 720 Northern Boulevard, Brookville, New York 11548, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4415","reference":[{"key":"25","doi-asserted-by":"crossref","unstructured":"M Kjelgaard-Hansen  and AL Jensen  \"Vet Clin Pathol.\"  35, \"Is the inherent imprecision of manual leukocyte differential counts acceptable for quantitative purposes?\" 268 (2006)","DOI":"10.1111\/j.1939-165X.2006.tb00128.x"},{"key":"7","doi-asserted-by":"crossref","unstructured":"AK Davis, DL Maney, JC Maerz,  \"Funct Ecol.\"  22, \"The use of leukocyte profiles to measure stress in vertebrates: a review for ecologists.\" 760 (2008)","DOI":"10.1111\/j.1365-2435.2008.01467.x"},{"key":"1","doi-asserted-by":"crossref","unstructured":"M Akhtar, M Hafeez, A Haq,  \"Int J Poult Sci.\"  4, \"Immunity against coccidiosis in poultry\u2014A review.\" 812 (2005)","DOI":"10.3923\/ijps.2005.812.817"},{"key":"3","doi-asserted-by":"crossref","unstructured":"A Berenjian, SD Sharifi, A Mohammadi-Sangcheshmeh, et al.  \"Biol Trace Elem Res.\"  184, \"Effect of chromium nanoparticles on physiological stress induced by exogenous dexamethasone in Japanese quails.\" 474 (2018)","DOI":"10.1007\/s12011-017-1192-y"},{"key":"4","doi-asserted-by":"crossref","unstructured":"HD. Chapman,  \"Poult Sci.\"  93, \"Milestones in avian coccidiosis research: A review.\" 501 (2014)","DOI":"10.3382\/ps.2013-03634"},{"key":"5","doi-asserted-by":"crossref","unstructured":"D C\u012brule, T Krama, J Vrublevska, et al.  \"J Ornithol.\"  153, \"A rapid effect of handling on counts of white blood cells in a wintering passerine bird: A more practical measure of stress?\" 161 (2012)","DOI":"10.1007\/s10336-011-0719-9"},{"key":"6","doi-asserted-by":"crossref","unstructured":"CA Cizauskas, WC Turner, N Pitts, et al.  \"PLoS One.\"  10, \"Seasonal patterns of hormones, macroparasites, and microparasites in wild African ungulates: The interplay among stress, reproduction, and disease.\" e0120800 (2015)","DOI":"10.1371\/journal.pone.0120800"},{"key":"8","doi-asserted-by":"crossref","unstructured":"R. de Matos,  \"Vet Clin North Am Exot Anim Pract.\"  11, \"Adrenal steroid metabolism in birds: Anatomy, physiology, and clinical considerations.\" 35 (2008)","DOI":"10.1016\/j.cvex.2007.09.006"},{"key":"9","doi-asserted-by":"crossref","unstructured":"FS Dhabhar, AH Miller, BS McEwen, et al.  \"J Immunol.\"  154, \"Effects of stress on immune cell distribution. Dynamics and hormonal mechanisms.\" 5511 (1995)","DOI":"10.4049\/jimmunol.154.10.5511"},{"key":"10","doi-asserted-by":"crossref","unstructured":"FS Dhabhar, AH Miller, BS McEwen, et al.  \"J Immunol.\"  157, \"Stress-induced changes in blood leukocyte distribution. Role of adrenal steroid hormones.\" 1638 (1996)","DOI":"10.4049\/jimmunol.157.4.1638"},{"key":"11","doi-asserted-by":"crossref","unstructured":"JE Dohms  and A Metz  \"Vet Immunol Immunopathol.\"  30, \"Stress\u2014Mechanisms of immunosuppression.\" 89 (1991)","DOI":"10.1016\/0165-2427(91)90011-Z"},{"key":"12","doi-asserted-by":"crossref","unstructured":"RB Evans-Storms  and JA Cidlowski  \"J Steroid Biochem Mol Biol.\"  53, \"Regulation of apoptosis by steroid hormones.\" 1 (1995)","DOI":"10.1016\/0960-0760(95)00034-W"},{"key":"13","doi-asserted-by":"crossref","unstructured":"X Fuentes-Arderiu, M Garc\u00eda-Panyella, D Dot-Bach,  \"Accredit Qual Assur.\"  12, \"Between-examiner reproducibility in manual differential leukocyte counting.\" 643 (2007)","DOI":"10.1007\/s00769-007-0323-0"},{"key":"14","doi-asserted-by":"crossref","unstructured":"M Giraudeau, M Mousel, S Earl, et al.  \"PLoS One.\"  9, \"Parasites in the city: Degree of urbanization predicts poxvirus and coccidian infections in house finches (Haemorhous mexicanus).\" e86747 (2014)","DOI":"10.1371\/journal.pone.0086747"},{"key":"15","doi-asserted-by":"crossref","unstructured":"RD Godfrey, AM Fedynich, DB Pence,  \"J Wildl Dis.\"  23, \"Quantification of hematozoa in blood smears.\" 558 (1987)","DOI":"10.7589\/0090-3558-23.4.558"},{"key":"16","doi-asserted-by":"crossref","unstructured":"JL Grodio, KV Dhondt, PH O'Connell, et al.  \"Avian Pathol.\"  37, \"Detection and quantification of Mycoplasma gallisepticum genome load in conjunctival samples of experimentally infected house finches (Carpodacus mexicanus) using real-time polymerase chain reaction.\" 385 (2008)","DOI":"10.1080\/03079450802216629"},{"key":"17","unstructured":"U Gr\u00f6ger  and F Grimm  \"Tierarztl Prax.\"  17, \"Dexamethason- und Prednisoloneinsatz bei Tauben [Dexamethasone and prednisolone use in pigeons].\" 167 (1989)"},{"key":"18","doi-asserted-by":"crossref","unstructured":"WB Gross, PB Siegel, RT DuBose,  \"Poult Sci.\"  59, \"Some effects of feeding corticosterone to chickens.\" 516 (1980)","DOI":"10.3382\/ps.0590516"},{"key":"19","doi-asserted-by":"crossref","unstructured":"L. Haas,  \"Die Auswerkung des Paramunit\u00e4tsinducers PIND ORF auf die Flugleistung sowie auf die Ausscheidung von Kokzidienoozysten von normalen und immunsupprimierten Brieftauben (Columba livia, Gmel., 1789, var. dom.) [The effect of the paramunity inducer PIND ORF on the racing pigeon performance and the excretion of oocysts on normal and immunosuppressed racing pigeons].\"  (1987)","DOI":"10.1111\/j.1439-0450.1987.tb00405.x"},{"key":"20","doi-asserted-by":"crossref","unstructured":"PM Hocking, MH Maxwell, GW Robertson, et al.  \"Br Poult Sci.\"  43, \"Welfare assessment of broiler breeders that are food restricted after peak rate of lay.\" 5 (2002)","DOI":"10.1080\/00071660120109818"},{"key":"21","doi-asserted-by":"crossref","unstructured":"P H\u00f5rak, M M\u00e4nniste, R Meitern, et al.  \"Gen Comp Endocrino.\"  191, \"Dexamethasone inhibits corticosterone deposition in feathers of greenfinches.\" 210 (2013)","DOI":"10.1016\/j.ygcen.2013.07.002"},{"key":"22","doi-asserted-by":"crossref","unstructured":"GR Huff, WE Huff, JM Balog, et al.  \"Poult Sci.\"  78, \"The effect of a second dexamethasone treatment on turkeys previously challenged in an experimental Escherichia coli respiratory model of turkey osteomyelitis complex.\" 1116 (1999)","DOI":"10.1093\/ps\/78.8.1116"},{"key":"23","doi-asserted-by":"crossref","unstructured":"GR Huff, WE Huff, NC Rath,  \"Avian Dis.\"  57, \"Dexamethasone immunosuppression resulting in turkey clostridial dermatitis: A retrospective analysis of seven studies, 1998\u20132009.\" 730 (2013)","DOI":"10.1637\/10522-030113-Reg.1"},{"key":"24","doi-asserted-by":"crossref","unstructured":"RB Jones, G Beuving, HJ Blokhuis,  \"Physiol Behav.\"  42, \"Tonic immobility and heterophil\/lymphocyte responses of the domestic fowl to corticosterone infusion.\" 249 (1988)","DOI":"10.1016\/0031-9384(88)90078-9"},{"key":"26","doi-asserted-by":"crossref","unstructured":"EA Koutsos  and KC Klasing  \"Avian immunology.\"  \"Factors modulating the avian immune system.\" 219 (2014)","DOI":"10.1016\/B978-0-12-396965-1.00017-0"},{"key":"27","doi-asserted-by":"crossref","unstructured":"I Krams, J Vrublevska, D Cirule, et al.  \"Ethology.\"  119, \"Stress, behaviour and immunity in wild-caught wintering great tits (Parus major).\" 397 (2013)","DOI":"10.1111\/eth.12075"},{"key":"28","unstructured":"MSG Lola, MGV Paraso, BP Divina, et al.  \"Philipp J Vet Anim Sci.\"  42, \"Heat stress induces histopathological changes in lymphoid organs of broiler and Philippine native chickens.\" 85 (2017)"},{"key":"29","unstructured":"JT. Lumeij,  \"Avian medicine: Principles and application.\"  \"Endocrinology.\" 582 (1994)"},{"key":"30","doi-asserted-by":"crossref","unstructured":"JM McFarlane  and SE Curtis  \"Poult Sci.\"  68, \"Multiple concurrent stressors in chicks: 3. Effects on plasma corticosterone and the heterophil:lymphocyte ratio.\" 522 (1989)","DOI":"10.3382\/ps.0680522"},{"key":"31","doi-asserted-by":"crossref","unstructured":"AE McRee, TN Tully, JG Nevarez, et al.  \"J Zoo Wildl Med.\"  49, \"Effect of routine handling and transportation on blood leukocyte concentrations and plasma corticosterone in captive Hispaniolan Amazon parrots (Amazona ventralis).\" 396 (2018)","DOI":"10.1638\/2016-0100.1"},{"key":"32","doi-asserted-by":"crossref","unstructured":"C M\u00fcller, S Jenni-Eiermann, L Jenni,  \"Funct Ecol.\"  25, \"Heterophils\/ lymphocytes-ratio and circulating corticosterone do not indicate the same stress imposed on Eurasian kestrel nestlings.\" 566 (2011)","DOI":"10.1111\/j.1365-2435.2010.01816.x"},{"key":"33","doi-asserted-by":"crossref","unstructured":"FN Nazar, EA Videla, ME Fernandez, et al.  \"Stress.\"  21, \"Insights into thermal stress in Japanese quail (Coturnix coturnix): Dynamics of immunoendocrine and biochemical responses during and after chronic exposure.\" 257 (2018)","DOI":"10.1080\/10253890.2018.1442430"},{"key":"34","doi-asserted-by":"crossref","unstructured":"B Ngasala  and S Bushukatale  \"Malar J.\"  18, \"Evaluation of malaria microscopy diagnostic performance at private health facilities in Tanzania.\" 375 (2019)","DOI":"10.1186\/s12936-019-2998-1"},{"key":"35","doi-asserted-by":"crossref","unstructured":"I Ots, A Murum\u00e4gi, P H\u00f5rak,  \"Funct Ecol.\"  12, \"Haematological health state indices of reproducing great tits: Methodology and sources of natural variation.\" 700 (1998)","DOI":"10.1046\/j.1365-2435.1998.00219.x"},{"key":"36","doi-asserted-by":"crossref","unstructured":"J Post, JMJ Rebel, AAHM ter Huurne,  \"Poult Sci.\"  82, \"Automated blood cell count: A sensitive and reliable method to study corticosterone-related stress in broilers.\" 591 (2003)","DOI":"10.1093\/ps\/82.4.591"},{"key":"37","doi-asserted-by":"crossref","unstructured":"C Power, N Fanning, HP Redmond,  \"Shock.\"  18, \"Cellular apoptosis and organ injury in sepsis: A review.\" 197 (2002)","DOI":"10.1097\/00024382-200209000-00001"},{"key":"38","doi-asserted-by":"crossref","unstructured":"MT Reinoso-P\u00e9rez, KV Dhondt, AV Sydenstricker, et al.  \"Ecol Evol.\"  10, \"Complex interactions between bacteria and haemosporidia in coinfected hosts: An experiment.\" 5801 (2020)","DOI":"10.1002\/ece3.6318"},{"key":"39","doi-asserted-by":"crossref","unstructured":"C Romeo, LA Wauters, F Santicchia, et al.  \"Curr Zool.\"  66, \"Complex relationships between physiological stress and endoparasite infections in natural populations.\" 449 (2020)","DOI":"10.1093\/cz\/zoaa029"},{"key":"40","doi-asserted-by":"crossref","unstructured":"JL Salak-Johnson  and JJ McGlone  \"J Anim Sci.\"  85, \"Making sense of apparently conflicting data: Stress and immunity in swine and cattle.\" E81 (2007)","DOI":"10.2527\/jas.2006-538"},{"key":"41","doi-asserted-by":"crossref","unstructured":"MA Sandhu, FQ Mirza, F Afzal, et al.  \"Livest Sci.\"  148, \"Effect of heat stress on cellular and humoral immunity and its cure with \u03b1-tocopherol in meat type birds.\" 181 (2012)","DOI":"10.1016\/j.livsci.2012.06.005"},{"key":"42","doi-asserted-by":"crossref","unstructured":"RM Sapolsky, LM Romero, AU Munck,  \"Endocr Rev.\"  21, \"How do glucocorticoids influence stress responses? Integrating permissive, suppressive, stimulatory, and preparative actions?\" 55 (2000)","DOI":"10.1210\/edrv.21.1.0389"},{"key":"43","doi-asserted-by":"crossref","unstructured":"LA Schoenle, IT Moore, AM Dudek, et al.  \"Proc Biol Sci.\"  286, \"Exogenous glucocorticoids amplify the costs of infection by reducing resistance and tolerance, but effects are mitigated by co-infection.\" 20182913 (2019)","DOI":"10.1098\/rspb.2018.2913"},{"key":"44","doi-asserted-by":"crossref","unstructured":"T Sepp, E Sild, P H\u00f5rak,  \"Physiol Biochem Zool.\"  83, \"Hematological condition indexes in greenfinches: Effects of captivity and diurnal variation.\" 276 (2010)","DOI":"10.1086\/648580"},{"key":"45","doi-asserted-by":"crossref","unstructured":"E Sild, R Meitern, M M\u00e4nniste, et al.  \"Gen Comp Endocrinol.\"  204, \"High feather corticosterone indicates better coccidian infection resistance in greenfinches.\" 203 (2014)","DOI":"10.1016\/j.ygcen.2014.05.026"},{"key":"46","doi-asserted-by":"crossref","unstructured":"SS Singh, SK Yadav, C Haldar,  \"Eur J Inflamm.\"  8, \"Effect of glucocorticoid and melatonin on immune function of an Indian tropical bird, Perdicula asiatica: An in vivo and in vitro study.\" 89 (2010)","DOI":"10.1177\/1721727X1000800206"},{"key":"47","unstructured":"\"Stata v15.1. Statistical software for data science.\"  (2020)"},{"key":"48","doi-asserted-by":"crossref","unstructured":"HW Tvedten  and IE Lillieh\u00f6\u00f6k  \"Vet Clin Pathol.\"  40, \"Canine differential leukocyte counting with the CellaVision DM96Vision, Sysmex XT-2000iV, and Advia 2120 hematology analyzers and a manual method.\" 324 (2011)","DOI":"10.1111\/j.1939-165X.2011.00347.x"},{"key":"49","doi-asserted-by":"crossref","unstructured":"G. Valki\u016bnas,  \"Avian malaria parasites and other haemosporidia.\"  946 (2005)","DOI":"10.1201\/9780203643792"},{"key":"50","doi-asserted-by":"crossref","unstructured":"CJ Walsh, JT Wyffels, AB Bodine, et al.  \"Dev Comp Immunol.\"  26, \"Dexamethasone-induced apoptosis in immune cells from peripheral circulation and lymphomyeloid tissues of juvenile clearnose skates, Raja eglanteria.\" 623 (2002)","DOI":"10.1016\/S0145-305X(02)00016-2"},{"key":"51","doi-asserted-by":"crossref","unstructured":"I Westerhof, WE Van den Brom, JA Mol, et al.  \"Avian Dis.\"  38, \"Sensitivity of the hypothalamicpituitary-adrenal system of pigeons (Columba livia domestica) to suppression by dexamethasone, cortisol, and prednisolone.\" 435 (1994)","DOI":"10.2307\/1592063"},{"key":"2","doi-asserted-by":"crossref","unstructured":"JE. Applegate,  \"J Parasitol.\"  56, \"Population changes in latent avian malaria infections associated with season and corticosterone treatment.\" 439 (1970)","DOI":"10.2307\/3277599"}],"container-title":["Journal of Wildlife Diseases"],"original-title":[],"deposited":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T07:04:57Z","timestamp":1793430297000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/journal-of-wildlife-diseases\/volume-58\/issue-3\/JWD-D-21-00129\/THE-EFFECT-OF-DEXAMETHASONE-ON-HEMATOLOGIC-PROFILES-HEMOSPORIDIAN-INFECTION-AND\/10.7589\/JWD-D-21-00129.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,25]]},"references-count":51,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2022,7,1]]}},"URL":"https:\/\/doi.org\/10.7589\/jwd-d-21-00129","relation":{},"ISSN":["0090-3558","1943-3700"],"issn-type":[{"value":"0090-3558","type":"print"},{"value":"1943-3700","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,25]]}}}