{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T05:33:28Z","timestamp":1793424808566,"version":"4.5.0"},"reference-count":59,"publisher":"Wildlife Disease Association","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Wildlife Diseases"],"published-print":{"date-parts":[[2023,10,24]]},"abstract":"<jats:p>Our understanding of wildlife multihost pathogen transmission systems is often incomplete due to the difficulty of observing contact between hosts. Understanding these interactions can be critical for preventing disease-induced wildlife declines. The proliferation of high-throughput sequencing technologies provides new opportunities to better explore these cryptic interactions. Parelaphostrongylus tenuis, a multihost parasite, is a leading cause of death in some moose (Alces alces) populations threatened by local extinction in the midwestern and northeastern US and southeastern Canada. Moose contract P. tenuis by consuming infected gastropod intermediate hosts, but little is known about which gastropod species moose consume. To gain more insight, we used a genetic metabarcoding approach on 258 georeferenced and temporally stratified moose fecal samples collected May\u2013October 2017\u201320 from a declining population in the north-central US. We detected moose consumption of three species of gastropods across five positive samples. Two of these (Punctum minutissimum and Helisoma sp.) have been minimally investigated for the ability to host P. tenuis, while one (Zonitoides arboreus) is a well-documented host. Moose consumption of gastropods documented herein occurred in June and September. Our findings prove that moose consume gastropod species known to become infected by P. tenuis and demonstrate that fecal metabarcoding can provide novel insight on interactions between hosts of a multispecies pathogen transmission system. After determining and improving test sensitivity, these methods may also be extended to document important interactions in other multihost disease systems.<\/jats:p>","DOI":"10.7589\/jwd-d-22-00120","type":"journal-article","created":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T06:38:36Z","timestamp":1698129516000},"page":"640-650","source":"Crossref","is-referenced-by-count":2,"title":["SPECIES IN THE FECES: DNA METABARCODING TO DETECT POTENTIAL GASTROPOD HOSTS OF PARELAPHOSTRONGYLUS TENUIS CONSUMED BY MOOSE (ALCES ALCES)"],"prefix":"10.7589","volume":"59","author":[{"given":"Tyler J.","family":"Garwood","sequence":"first","affiliation":[{"name":"Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, 1988 Fitch Avenue, St. Paul, Minnesota 55108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seth A.","family":"Moore","sequence":"additional","affiliation":[{"name":"Department of Biology and Environment, Grand Portage Band of Lake Superior Chippewa, 27 Store Road, Grand Portage, Minnesota 55605, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas M.","family":"Fountain-Jones","sequence":"additional","affiliation":[{"name":"School of Natural Sciences, University of Tasmania, Private Bag 37, Hobart, Tasmania 7001, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter A.","family":"Larsen","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, 1971 Commonwealth Avenue, Saint Paul, Minnesota 55108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiffany M.","family":"Wolf","sequence":"additional","affiliation":[{"name":"Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, 1988 Fitch Avenue, St. Paul, Minnesota 55108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4415","reference":[{"key":"42","unstructured":"\"Basic local alignment search tool.\"  (2022)"},{"key":"52","doi-asserted-by":"publisher","unstructured":"A. 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