{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T05:31:19Z","timestamp":1793424679730,"version":"4.5.0"},"reference-count":24,"publisher":"Wildlife Disease Association","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Wildlife Diseases"],"published-print":{"date-parts":[[2024,1,12]]},"abstract":"<jats:p>Coyotes (Canis latrans) rapidly expanded across North America during the 20th century and in 1987 colonized insular Newfoundland, Canada. Their arrival brought the potential for new predator-prey interactions and the potential for transmission of parasites to na\u00efve populations. Trichinella spp. and Echinococcus spp. are zoonotic parasites not previously reported from the island of Newfoundland, Canada. Muscle samples (diaphragm and tongue) from 153 coyotes and feces from 35\/153 coyotes were collected. Larvae of Trichinella spp. were recovered by muscle digestion from 6\/153 coyotes (3.9%) and identified using multiplex PCR and Sanger sequencing as T. nativa. Fecal samples were screened for DNA of Echinococcus spp. using qPCR, and intestines from positive animals were examined for adult cestodes. No fecal samples were positive for DNA of E. multilocularis, and 2\/35 (5.7%) samples were positive for E. canadensis, of which one was successfully genotyped as the G10 cervid strain. Echinococcus canadensis has not previously been reported on the island of Newfoundland, historically the only region of Canada where Echinococcus spp. was not known to occur. No species of Trichinella have previously been reported on the island. Both parasites are zoonotic, and hunters, trappers, dog owners, and the general public should be aware of these new risks for public health.<\/jats:p>","DOI":"10.7589\/jwd-d-23-00084","type":"journal-article","created":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T02:22:11Z","timestamp":1705026131000},"page":"211-215","source":"Crossref","is-referenced-by-count":4,"title":["New Geographic Records for Trichinella nativa and Echinococcus canadensis in Coyotes (Canis latrans) from Insular Newfoundland, Canada"],"prefix":"10.7589","volume":"60","author":[{"given":"Cody J.","family":"Malone","sequence":"first","affiliation":[{"name":"Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, S7N 5B4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Temitope U.","family":"Kolapo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, S7N 5B4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugh","family":"Whitney","sequence":"additional","affiliation":[{"name":"Department of Biology, Memorial University of Newfoundland, 45 Arctic Avenue, St. John's, Newfoundland and Labrador, A1C 5S7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Callahan","sequence":"additional","affiliation":[{"name":"Fisheries, Forestry and Agriculture, Government of Newfoundland and Labrador, 192 Wheeler's Road, Corner Brook, P.O. Box 2006, Newfoundland and Labrador, A2H 0J1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shane","family":"Hann","sequence":"additional","affiliation":[{"name":"Fisheries, Forestry and Agriculture, Government of Newfoundland and Labrador, 192 Wheeler's Road, Corner Brook, P.O. Box 2006, Newfoundland and Labrador, A2H 0J1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Don","family":"Keefe","sequence":"additional","affiliation":[{"name":"Fisheries, Forestry and Agriculture, Government of Newfoundland and Labrador, 192 Wheeler's Road, Corner Brook, P.O. Box 2006, Newfoundland and Labrador, A2H 0J1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Jenkins","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan, S7N 5B4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4415","reference":[{"key":"17","doi-asserted-by":"crossref","unstructured":"E. Pozio,  \"Food Waterborne Parasitol.\"  4, \"Adaptation of Trichinella spp. for survival in cold climates.\" 4 (2016)","DOI":"10.1016\/j.fawpar.2016.07.001"},{"key":"8","doi-asserted-by":"publisher","unstructured":"J Eckert  and P Deplazes  \"Clin Microbiol Rev.\"  17, \"Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern.\" 107 (2004)","DOI":"10.1128\/cmr.17.1.107-135.2004"},{"key":"1","doi-asserted-by":"crossref","unstructured":"AA. Aguirre,  \"Parasites Vectors.\"  2, \"Wild canids as sentinels of ecological health: a conservation medicine perspective.\" S7 (2009)","DOI":"10.1186\/1756-3305-2-S1-S7"},{"key":"2","doi-asserted-by":"crossref","unstructured":"GD Appleyard  and AA Gajadhar  \"Can J Public Health.\"  91, \"A review of trichinellosis in people and wildlife in Canada.\" 293 (2000)","DOI":"10.1007\/BF03404292"},{"key":"3","unstructured":"J Blake,  \"Coyotes in insular Newfoundland.\"  11 (2006)"},{"key":"5","doi-asserted-by":"crossref","unstructured":"J Bowles  and DP McManus  \"Int J Parasitol.\"  23, \"NADH dehydrogenase 1 gene sequences compared for species and strains of the genus Echinococcus.\" 969 (1993)","DOI":"10.1016\/0020-7519(93)90065-7"},{"key":"6","doi-asserted-by":"crossref","unstructured":"CE Butler  and RA Khan  \"J Wildl Dis.\"  28, \"Prevalence of Trichinella spiralis in black bears (Ursus americanus) from Newfoundland and Labrador, Canada.\" 474 (1992)","DOI":"10.7589\/0090-3558-28.3.474"},{"key":"7","doi-asserted-by":"crossref","unstructured":"P Deplazes, L Rinaldi, CA Alvarez Rojas, et al.  \"Adv Parasitol.\"  95, \"Global distribution of alveolar and cystic echinococcosis.\" 315 (2017)","DOI":"10.1016\/bs.apar.2016.11.001"},{"key":"9","doi-asserted-by":"crossref","unstructured":"LB Forbes  and AA Gajadhar  \"J Food Prot.\"  62, \"A validated Trichinella digestion assay and an associated sampling and quality assurance system for use in testing pork and horse meat.\" 1308 (1999)","DOI":"10.4315\/0362-028X-62.11.1308"},{"key":"11","doi-asserted-by":"crossref","unstructured":"B Gottstein, E Pozio, K N\u00f6ckler,  \"Clin Microbiol Rev.\"  22, \"Epidemiology, diagnosis, treatment, and control of trichinellosis.\" 127 (2009)","DOI":"10.1128\/CMR.00026-08"},{"key":"12","unstructured":"\"Animal disease studies in Newfoundland and Labrador\u2014an annotated bibliography (Internal Report).\"  (2021)"},{"key":"14","doi-asserted-by":"crossref","unstructured":"J Karamon, J Sroka, T Cencek,  \"Exp Parasitol.\"  124, \"Limit of detection of sedimentation and counting technique (SCT) for Echinococcus multilocularis diagnosis, estimated under experimental conditions.\" 244 (2010)","DOI":"10.1016\/j.exppara.2009.09.007"},{"key":"15","unstructured":"TU Kolapo,  \"Molecular epidemiology and diagnostics for Echinococcus multilocularis in canid definitive and intermediate hosts.\"  54 (2023)"},{"key":"18","doi-asserted-by":"crossref","unstructured":"E Pozio  and D Zarlenga  \"Food Waterborne Parasitol.\"  15, \"International Commission on Trichinellosis: recommendations for genotyping Trichinella muscle stage larvae.\" e00033 (2019)","DOI":"10.1016\/j.fawpar.2018.e00033"},{"key":"19","doi-asserted-by":"crossref","unstructured":"JM Priest, DL McRuer, DT Stewart, et al.  \"Int J Parasitol Parasites Wildl.\"  16, \"New geographic records for Echinococcus canadensis in coyotes and moose from Nova Scotia, Canada.\" 285 (2021)","DOI":"10.1016\/j.ijppaw.2021.11.004"},{"key":"20","unstructured":"WT Robbins, O Galeuzzi, K Graham, et al.  \"Can Vet J.\"  63, \"Echinococcus multilocularis infection in a red fox (Vulpes vulpes) on Prince Edward Island, Canada.\" 962 (2022)"},{"key":"21","doi-asserted-by":"crossref","unstructured":"MA Santa, M Musiani, KE Ruckstuhl, et al.  \"Parasitology.\"  148, \"A review on invasions by parasites with complex life cycles: the European strain of Echinococcus multilocularis in North America as a model.\" 1532 (2021)","DOI":"10.1017\/S0031182021001426"},{"key":"22","doi-asserted-by":"crossref","unstructured":"J Schurer, T Shury, F Leighton, et al.  \"Int J Parasitol Parasites Wildl.\"  2, \"Surveillance for Echinococcus canadensis genotypes in Canadian ungulates.\" 97 (2013)","DOI":"10.1016\/j.ijppaw.2013.02.004"},{"key":"23","doi-asserted-by":"crossref","unstructured":"R Sharma, PC Thompson, EP Hoberg, et al.  \"Int J Parasitol.\"  50, \"Hiding in plain sight: discovery and phylogeography of a cryptic species of Trichinella (Nematoda: Trichinellidae) in wolverine (Gulo gulo).\" 277 (2020)","DOI":"10.1016\/j.ijpara.2020.01.003"},{"key":"24","unstructured":"D. Snow,  \"Land mammals of Newfoundland and Labrador.\"  23 (1996)"},{"key":"4","doi-asserted-by":"crossref","unstructured":"J Bowles, D Blair, DP McManus,  \"Mol Biochem Parasitol.\"  54, \"Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing.\" 165 (1992)","DOI":"10.1016\/0166-6851(92)90109-W"},{"key":"10","doi-asserted-by":"crossref","unstructured":"K Gesy, M Pawlik, L Kapronczai, et al.  \"Parasitol Res.\"  112, \"An improved method for the extraction and quantification of adult Echinococcus from wildlife definitive hosts.\" 2075 (2013)","DOI":"10.1007\/s00436-013-3371-x"},{"key":"13","doi-asserted-by":"crossref","unstructured":"JW Hody  and R Kays  \"Zookeys.\"  759, \"Mapping the expansion of coyotes (Canis latrans) across North and Central America.\" 81 (2018)","DOI":"10.3897\/zookeys.759.15149"},{"key":"16","doi-asserted-by":"crossref","unstructured":"JD Kotwa, M Isaksson, CM Jardine, et al.  \"Emerg Infect Dis.\"  25, \"Echinococcus multilocularis infection, southern Ontario, Canada.\" 265 (2019)","DOI":"10.3201\/eid2502.180299"}],"container-title":["Journal of Wildlife Diseases"],"original-title":[],"deposited":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T04:48:21Z","timestamp":1793422101000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/journal-of-wildlife-diseases\/volume-60\/issue-1\/JWD-D-23-00084\/New-Geographic-Records-for-Trichinella-nativa-and-Echinococcus-canadensis-in\/10.7589\/JWD-D-23-00084.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,12]]},"references-count":24,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,1,1]]}},"URL":"https:\/\/doi.org\/10.7589\/jwd-d-23-00084","relation":{},"ISSN":["0090-3558","1943-3700"],"issn-type":[{"value":"0090-3558","type":"print"},{"value":"1943-3700","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,12]]}}}