{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T04:32:30Z","timestamp":1793421150852,"version":"4.5.0"},"reference-count":41,"publisher":"Wildlife Disease Association","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Wildlife Diseases"],"published-print":{"date-parts":[[2024,4,5]]},"abstract":"<jats:p>The order Procellariiformes includes several species of seabirds that perform long-distance migrations crossing all the oceans. These movements may contribute to the dispersal and exchange of hemoparasites, such as haemosporidians. There is a lack of studies regarding the order Haemosporida in Procellariiformes, and, to date, only the genus Plasmodium has been reported. This survey investigated the occurrence of the three genera of haemosporidians, Plasmodium, Haemoproteus, and Leucocytozoon, in samples collected between 2013 and 2022 from 95 individuals of 14 species of Procellariiformes from southern Brazil, including live animals in rehabilitation centers, individuals caught as incidental bycatch, and carcasses found along the coast. A total of 171 samples of blood and fragments of liver and spleen were analyzed, with extracted DNA being subjected to a nested PCR followed by phylogeny analysis. All animals were negative for Plasmodium spp. and Leucocytozoon spp., but one Black-browed Albatross (Thalassarche melanophris) and one Manx Shearwater (Puffinus puffinus) specimen were positive for Haemoproteus spp. The sequences obtained from positive seabirds did not show 100% similarity with other known lineages available in the MalAvi database and thus were probably novel lineages. However, one sequence clustered together with Haemoproteus noctuae, a parasite from Strigiformes, while the other was grouped with Haemoproteus columbae, which is classically related to Columbiformes. These results suggest that both positive animals may have become infected when beached or in rehabilitation centers by a spillover of vectors from local birds. This highlights the importance of surveillance of the health of Procellariiformes regarding the possibility of dissemination of new pathogens in different bird populations.<\/jats:p>","DOI":"10.7589\/jwd-d-23-00087","type":"journal-article","created":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T13:53:46Z","timestamp":1713534826000},"page":"413-420","source":"Crossref","is-referenced-by-count":5,"title":["MOLECULAR SURVEY OF HAEMOSPORIDIAN PARASITES IN PROCELLARIIFORMES SAMPLED IN SOUTHERN BRAZIL, 2013\u201322"],"prefix":"10.7589","volume":"60","author":[{"given":"Annelise Zabel","family":"Sgarioni","sequence":"first","affiliation":[{"name":"Instituto de Pesquisas Veterin\u00e1rias Desid\u00e9rio Finamor (IPVDF), Estrada do Conde, 6000, Eldorado do Sul, 92990-000, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Serafini","sequence":"additional","affiliation":[{"name":"Centro Nacional de Pesquisa e Conserva\u00e7\u00e3o de Aves Silvestres (CEMAVE), Instituto Chico Mendes de Conserva\u00e7\u00e3o da Biodiversidade (ICMBio), Rodovia Maur\u00edcio Sirotski Sobrinho s\/n, Florian\u00f3polis, 88053-700, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice","family":"Pereira","sequence":"additional","affiliation":[{"name":"Projeto Albatroz, Rodovia SC 402, Km 2, Rio Vermelho, Florian\u00f3polis, 88061-500, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiffany","family":"Emmerich","sequence":"additional","affiliation":[{"name":"Unidade de Estabiliza\u00e7\u00e3o de Animais Marinhos, Universidade do Vale de Itaja\u00ed (UNIVALI), Avenida Itacolumi, 228, Penha, 88385-000, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thamires Pires","family":"de Pontes","sequence":"additional","affiliation":[{"name":"Unidade de Estabiliza\u00e7\u00e3o de Animais Marinhos, Universidade do Vale de Itaja\u00ed (UNIVALI), Avenida Itacolumi, 228, Penha, 88385-000, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas Coutinho","family":"Machado","sequence":"additional","affiliation":[{"name":"Unidade de Estabiliza\u00e7\u00e3o de Animais Marinhos, Universidade do Vale de Itaja\u00ed (UNIVALI), Avenida Itacolumi, 228, Penha, 88385-000, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula Reis","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Setor de Patologia Veterin\u00e1ria, Departamento de Patologia Cl\u00ednica Veterin\u00e1ria, Faculdade de Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul (UFRGS), Avenida Bento Gon\u00e7alves, 9090, Porto Alegre, 90650-001, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek Blaese","family":"de Amorim","sequence":"additional","affiliation":[{"name":"Setor de Patologia Veterin\u00e1ria, Departamento de Patologia Cl\u00ednica Veterin\u00e1ria, Faculdade de Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul (UFRGS), Avenida Bento Gon\u00e7alves, 9090, Porto Alegre, 90650-001, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilherme","family":"Klafke","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisas Veterin\u00e1rias Desid\u00e9rio Finamor (IPVDF), Estrada do Conde, 6000, Eldorado do Sul, 92990-000, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Reck","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisas Veterin\u00e1rias Desid\u00e9rio Finamor (IPVDF), Estrada do Conde, 6000, Eldorado do Sul, 92990-000, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4415","reference":[{"key":"32","doi-asserted-by":"crossref","unstructured":"K Tamura, G Stecher, S Kumar,  \"Mol Biol Evol.\"  38, \"MEGA11: Molecular Evolutionary Genetics Analysis Version 11.\" 3022 (2021)","DOI":"10.1093\/molbev\/msab120"},{"key":"18","unstructured":"A Mart\u00ednez-Abra\u00edn, B Esparza, D Oro,  \"Ardeola.\"  51, \"Lack of blood parasites in bird species: does absence of blood parasite vectors explain it all?\" 225 (2004)"},{"key":"26","doi-asserted-by":"crossref","unstructured":"P Quillfeldt, J Mart\u00ednez, L Bugoni, et al.  \"Parasitology.\"  141, \"Blood parasites in noddies and boobies from Brazilian offshore islands\u2014differences between species and influence of nesting habitat.\" 399 (2014)","DOI":"10.1017\/S0031182013001649"},{"key":"10","doi-asserted-by":"crossref","unstructured":"O Hellgren, J Waldenstr\u00f6m, S Bensch,  \"J Parasitol.\"  90, \"A new PCR assay for simultaneous studies of Leucocytozoon, Plasmodium, and Haemoproteus from avian blood.\" 797 (2004)","DOI":"10.1645\/GE-184R1"},{"key":"31","doi-asserted-by":"crossref","unstructured":"K Tamura, D Peterson, N Peterson, et al.  \"Mol Biol Evol.\"  28, \"MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.\" 2731 (2011)","DOI":"10.1093\/molbev\/msr121"},{"key":"40","doi-asserted-by":"crossref","unstructured":"RE. Warner,  \"Condor.\"  70, \"The role of introduced diseases in the extinction of the endemic Hawaiian avifauna.\" 101 (1968)","DOI":"10.2307\/1365954"},{"key":"7","doi-asserted-by":"crossref","unstructured":"MP Dias, R Martin, EJ Pearmain, et al.  \"Biol Conserv.\"  237, \"Threats to seabirds: A global assessment.\" 525 (2019)","DOI":"10.1016\/j.biocon.2019.06.033"},{"key":"34","doi-asserted-by":"crossref","unstructured":"G. Valki\u016bnas,  \"Avian malaria parasites and other haemosporidia.\"  946 (2005)","DOI":"10.1201\/9780203643792"},{"key":"24","doi-asserted-by":"crossref","unstructured":"RA Phillips, R Gales, GB Baker, et al.  \"Biol Conserv.\"  201, \"The conservation status and priorities for albatrosses and large petrels.\" 169 (2016)","DOI":"10.1016\/j.biocon.2016.06.017"},{"key":"1","doi-asserted-by":"crossref","unstructured":"LT. Ballance,  \"Mar Ornithol.\"  35, \"Understanding seabirds at sea: Why and how?\" 127 (2007)","DOI":"10.5038\/2074-1235.35.2.743"},{"key":"2","doi-asserted-by":"crossref","unstructured":"A Bataille, AA Cunningham, V Cede\u00f1o, et al.  \"Proc R Soc B Biol Sci.\"  276, \"Evidence for regular ongoing introductions of mosquito disease vectors into the Gal\u00e1pagos Islands.\" 3769 (2009)","DOI":"10.1098\/rspb.2009.0998"},{"key":"3","unstructured":"M Beal, MP Dias, RA Phillips, et al.  \"Sci Adv.\"  7, \"Global political responsibility for the conservation of albatrosses and large petrels.\" eabd7225 (2021)"},{"key":"5","doi-asserted-by":"crossref","unstructured":"L Campioni, J Mart\u00ednez-de la Puente, J Figuerola, et al.  \"Parasitol Res.\"  117, \"Absence of haemosporidian parasite infections in the long-lived Cory's shearwater: Evidence from molecular analyses and review of the literature.\" 323 (2018)","DOI":"10.1007\/s00436-017-5676-7"},{"key":"6","doi-asserted-by":"crossref","unstructured":"BL Cannell, KV Krasnec, K Campbell, et al.  \"Vet Parasitol.\"  197, \"The pathology and pathogenicity of a novel Haemoproteus spp. infection in wild little penguins (Eudyptula minor).\" 74 (2013)","DOI":"10.1016\/j.vetpar.2013.04.025"},{"key":"8","doi-asserted-by":"crossref","unstructured":"M Dietrich, E G\u00f3mez-D\u00edaz, KD McCoy,  \"Vector Borne Zoonotic Dis.\"  11, \"Worldwide distribution and diversity of seabird ticks: Implications for the ecology and epidemiology of tick-borne pathogens.\" 453 (2011)","DOI":"10.1089\/vbz.2010.0009"},{"key":"9","unstructured":"B Esparza, A Mart\u00ednez-Abra\u00edn, S Merino, et al.  \"Ornis Fennica.\"  81, \"Immunocompetence and prevalence of haematozoan parasites in two long-lived seabirds.\" 40 (2004)"},{"key":"11","doi-asserted-by":"crossref","unstructured":"L Ilahiane, F De Pascalis, D Pisu, et al.  \"Mar Ornithol.\"  50, \"No evidence of avian malaria in two Mediterranean endemic seabirds.\" 13 (2022)","DOI":"10.5038\/2074-1235.50.1.1454"},{"key":"12","unstructured":"\"The IUCN Red List of Threatened Species of 2022-2.\"  (2022)"},{"key":"13","doi-asserted-by":"crossref","unstructured":"M Inumaru, K Murata, Y Sato,  \"Int J Parasitol Parasites Wildl.\"  6, \"Prevalence of avian haemosporidia among injured wild birds in Tokyo and environs, Japan.\" 299 (2017)","DOI":"10.1016\/j.ijppaw.2017.09.007"},{"key":"14","doi-asserted-by":"crossref","unstructured":"B Kleinschmidt, M Dorsch, S Hein\u00e4nen, et al.  \"Microorganisms.\"  10, \"Prevalence of haemosporidian parasites in an arctic breeding seabird species\u2014The red-throated Diver (Gavia stellata).\" 2147 (2022)","DOI":"10.3390\/microorganisms10112147"},{"key":"15","doi-asserted-by":"crossref","unstructured":"IA Lotta, G Valki\u016bnas, MA Pacheco, et al.  \"Int J Parasitol Parasites Wildl.\"  9, \"Disentangling Leucocytozoon parasite diversity in the neotropics: Descriptions of two new species and shortcomings of molecular diagnostics for leucocytozoids.\" 159 (2019)","DOI":"10.1016\/j.ijppaw.2019.05.002"},{"key":"16","doi-asserted-by":"crossref","unstructured":"TA Maia, ST Vila\u00e7a, LR da Silva, et al.  \"Genet Mol Biol.\"  40, \"DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian merganser.\" 808 (2017)","DOI":"10.1590\/1678-4685-gmb-2016-0297"},{"key":"17","doi-asserted-by":"crossref","unstructured":"PN Mariano  and GPM Dantas  \"Mar Ornithol.\"  49, \"Low prevalence of Plasmodium and Haemoproteus in resident seabirds from oceanic islands off the Brazilian coast.\" 97 (2021)","DOI":"10.5038\/2074-1235.49.1.1404"},{"key":"19","doi-asserted-by":"crossref","unstructured":"KD McCoy, M Dietrich, A Jaeger, et al.  \"Acta Oecol.\"  72, \"The role of seabirds of the Iles Eparses as reservoirs and disseminators of parasites and pathogens.\" 98 (2016)","DOI":"10.1016\/j.actao.2015.12.013"},{"key":"20","doi-asserted-by":"crossref","unstructured":"AP Morel, A Webster, F Prusch, et al.  \"Vet Parasitol Reg Stud Rep.\"  23, \"Molecular detection and phylogenetic relationship of Haemosporida parasites in free-ranging wild raptors from Brazil.\" 100521 (2021)","DOI":"10.1016\/j.vprsr.2020.100521"},{"key":"21","unstructured":"D Onley  and P Scofield  \"Albatrosses, petrels, and shearwaters of the world.\"  240 (2007)"},{"key":"22","doi-asserted-by":"publisher","unstructured":"JF Pacheco, LF Silveira, A Aleixo, et al.  \"Ornithol Res.\"  29, \"Annotated checklist of the birds of Brazil by the Brazilian Ornithological Records Committee\u2014Second edition.\" 94 (2021)","DOI":"10.1007\/s43388-021-00058-x"},{"key":"23","doi-asserted-by":"crossref","unstructured":"NJ Parsons, NM Voogt, AM Schaefer, et al.  \"Vet Parasitol.\"  233, \"Occurrence of blood parasites in seabirds admitted for rehabilitation in the Western Cape, South Africa, 2001\u20132013.\" 52 (2017)","DOI":"10.1016\/j.vetpar.2016.12.001"},{"key":"25","doi-asserted-by":"crossref","unstructured":"P Quillfeldt, E Arriero, J Mart\u00ednez, et al.  \"Front Zool.\"  8, \"Prevalence of blood parasites in seabirds\u2014A review.\" 26 (2011)","DOI":"10.1186\/1742-9994-8-26"},{"key":"27","doi-asserted-by":"crossref","unstructured":"P Quillfeldt, J Mart\u00ednez, J Hennicke, et al.  \"Naturwissenschaften.\"  97, \"Hemosporidian blood parasites in seabirds\u2014A comparative genetic study of species from Antarctic to tropical habitats.\" 809 (2010)","DOI":"10.1007\/s00114-010-0698-3"},{"key":"28","doi-asserted-by":"crossref","unstructured":"N Sallaberry-Pincheira, D Gonzalez-Acu\u00f1a, Y Herrera-Tello, et al.  \"Ecohealth.\"  12, \"Molecular epidemiology of avian malaria in wild breeding colonies of Humboldt and Magellanic penguins in South America.\" 267 (2015)","DOI":"10.1007\/s10393-014-0995-y"},{"key":"29","unstructured":"J Sambrook, ER Fritsch, T Maniatis,  \"Molecular cloning: A laboratory manual.\"  (1989)"},{"key":"33","doi-asserted-by":"crossref","unstructured":"MM Uhart, L Gallo, F Quintana,  \"Bird Conserv Int.\"  28, \"Review of diseases (pathogen isolation, direct recovery, and antibodies) in albatrosses and large petrels worldwide.\" 169 (2018)","DOI":"10.1017\/S0959270916000629"},{"key":"35","doi-asserted-by":"crossref","unstructured":"G Valki\u016bnas  and TA Iezhova  \"Malar J.\"  21, \"Keys to the avian Haemoproteus parasites (Haemosporida, Haemoproteidae).\" 269 (2022)","DOI":"10.1186\/s12936-022-04235-1"},{"key":"36","doi-asserted-by":"crossref","unstructured":"RET Vanstreels, RP da Silva-Filho, CKM Kolesnikovas, et al.  \"Vet Res.\"  46, \"Epidemiology and pathology of avian malaria in penguins undergoing rehabilitation in Brazil.\" 30 (2015)","DOI":"10.1186\/s13567-015-0160-9"},{"key":"37","doi-asserted-by":"crossref","unstructured":"RET Vanstreels, DA Dutra, AP Santos, et al.  \"Parasitol Int.\"  78, \"First report of avian malaria in a Manx shearwater (Puffinus puffinus).\" 102148 (2020)","DOI":"10.1016\/j.parint.2020.102148"},{"key":"38","doi-asserted-by":"crossref","unstructured":"RET Vanstreels, FR Miranda, V Ruoppolo, et al.  \"Polar Biol.\"  37, \"Investigation of blood parasites of pygoscelid penguins at the King George and Elephant Islands, South Shetlands Archipelago, Antarctica.\" 135 (2014)","DOI":"10.1007\/s00300-013-1401-x"},{"key":"39","unstructured":"J. Warham,  \"The behaviour, population biology and physiology of the petrels.\"  613 (1996)"},{"key":"41","doi-asserted-by":"crossref","unstructured":"M Yoshimoto, K Ozawa, H Kondo, et al.  \"Parasitol Res.\"  120, \"A fatal case of a captive snowy owl (Bubo scandiacus) with Haemoproteus infection in Japan.\" 277 (2020)","DOI":"10.1007\/s00436-020-06972-1"},{"key":"4","doi-asserted-by":"crossref","unstructured":"S Bensch, O Hellgren, J P\u00e9rez-Tris,  \"Mol Ecol Resour.\"  9, \"MalAvi: A public database of malaria parasites and related haemosporidians in avian hosts based on mitochondrial cytochrome b lineages.\" 1353 (2009)","DOI":"10.1111\/j.1755-0998.2009.02692.x"},{"key":"30","doi-asserted-by":"crossref","unstructured":"EW Sayers, EE Bolton, JR Brister, et al.  \"Nucleic Acids Res.\"  50, \"Database resources of the national center for biotechnology information.\" D20 (2022)","DOI":"10.1093\/nar\/gkab1112"}],"container-title":["Journal of Wildlife Diseases"],"original-title":[],"deposited":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T04:26:19Z","timestamp":1793420779000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/journal-of-wildlife-diseases\/volume-60\/issue-2\/JWD-D-23-00087\/MOLECULAR-SURVEY-OF-HAEMOSPORIDIAN-PARASITES-IN-PROCELLARIIFORMES-SAMPLED-IN-SOUTHERN\/10.7589\/JWD-D-23-00087.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,5]]},"references-count":41,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,4,1]]}},"URL":"https:\/\/doi.org\/10.7589\/jwd-d-23-00087","relation":{},"ISSN":["0090-3558","1943-3700"],"issn-type":[{"value":"0090-3558","type":"print"},{"value":"1943-3700","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,5]]}}}