{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:54:37Z","timestamp":1791244477709,"version":"4.1.0"},"reference-count":45,"publisher":"Wildlife Disease Association","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Wildlife Diseases"],"published-print":{"date-parts":[[2025,5,16]]},"abstract":"<jats:p>Sulawesi tortoise adenovirus (STADV) has been implicated in a mortality event involving three endangered species of tortoises and detected in free-living painted turtles (Chrysemys picta); however, the epidemiology of this virus in North American turtles remains unclear. The specific objectives of this study were to 1) estimate the prevalence of STADV in free-living Blanding's turtles (Emydoidea blandingii), painted turtles, and red-eared sliders (RES; Trachemys scripta elegans) in Illinois, US, using quantitative PCR (qPCR) and 2) determine if qPCR prevalence is associated with location, sex, age class, blood parameters, or clinical signs across these three species. Oral-cloacal swabs from 581 Blanding's turtles, 137 painted turtles, and 82 RES sampled across three counties from 2017 to 2022 were evaluated via qPCR. The prevalence of STADV was 2.4% for Blanding's turtles, 14.9% for painted turtles, and 45% for RES. Blanding's turtle subadults (P=0.022) and painted turtle adults (P&lt;0.0001) were more likely to test positive than other age classes within the same species, while no age class association was found in RES (P=0.5). Clinical signs associated with STADV detection included quiet, alert, responsive mentation (P=0.002), pink mucous membranes (P&lt;0.001), carapacial abnormalities (P=0.036), and plastron abnormalities (P=0.003). In a multivariable model, significant predictors for STADV detection included species, with painted turtles (P&lt;0.0001) and RES (P=0.001) more likely to test positive than Blanding's turtles, and year, with turtles sampled in 2021 less likely to test positive than those sampled in 2017 (P=0.005). Detection of STADV was not significantly associated with location, month, sex, hematologic findings, serum biochemical findings, or protein electrophoretic profiles. Widespread molecular detection of STADV in these three species suggests a possible origin for the virus in the outbreak described in 2009, underscoring the importance of epidemiology studies to aid in the management of free-living and managed chelonians.<\/jats:p>","DOI":"10.7589\/jwd-d-24-00124","type":"journal-article","created":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T00:28:21Z","timestamp":1747355301000},"page":"408-418","source":"Crossref","is-referenced-by-count":1,"title":["EPIDEMIOLOGY OF SULAWESI TORTOISE ADENOVIRUS IN FREE-LIVING BLANDING'S TURTLES (EMYDOIDEA BLANDINGII), PAINTED TURTLES (CHRYSEMYS PICTA), AND RED-EARED SLIDERS (TRACHEMYS SCRIPTA ELEGANS) IN ILLINOIS, USA"],"prefix":"10.7589","volume":"61","author":[{"given":"Zachary C.","family":"Ready","sequence":"first","affiliation":[{"name":"Wildlife Epidemiology Laboratory, Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Veterinary Medicine Basic Sciences Building, 2001 South Lincoln Avenue, Urbana, Illinois 61802, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Adamovicz","sequence":"additional","affiliation":[{"name":"Wildlife Epidemiology Laboratory, Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Veterinary Medicine Basic Sciences Building, 2001 South Lincoln Avenue, Urbana, Illinois 61802, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maris","family":"Daleo","sequence":"additional","affiliation":[{"name":"Wildlife Epidemiology Laboratory, Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Veterinary Medicine Basic Sciences Building, 2001 South Lincoln Avenue, Urbana, Illinois 61802, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amber","family":"Simmons","sequence":"additional","affiliation":[{"name":"Wildlife Epidemiology Laboratory, Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Veterinary Medicine Basic Sciences Building, 2001 South Lincoln Avenue, Urbana, Illinois 61802, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Glowacki","sequence":"additional","affiliation":[{"name":"Lake County Forest Preserve District, 1899 West Winchester Road, Libertyville, Illinois 60048, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Graser","sequence":"additional","affiliation":[{"name":"Forest Preserve District of Kane County, 1996 South Kirk Road, Geneva, Illinois 60134, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Anchor","sequence":"additional","affiliation":[{"name":"Forest Preserves of Cook County, 536 North Harlem Avenue, River Forest, Illinois 60305, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Thompson","sequence":"additional","affiliation":[{"name":"DuPage County Forest Preserve District, 3S580 Naperville Road, Wheaton, Illinois 60189, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew C.","family":"Allender","sequence":"additional","affiliation":[{"name":"Wildlife Epidemiology Laboratory, Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois, Veterinary Medicine Basic Sciences Building, 2001 South Lincoln Avenue, Urbana, Illinois 61802, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4415","reference":[{"key":"2","doi-asserted-by":"crossref","unstructured":"MC Allender, M Abd-Eldaim, A Kuhns, et al.  \"J Herpetol Med Surg.\"  19, \"Absence of ranavirus and herpesvirus in a survey of two aquatic turtle species in Illinois.\" 16 (2009)","DOI":"10.5818\/1529-9651.19.1.16"},{"key":"27","doi-asserted-by":"crossref","unstructured":"EM Newman, MC Allender, D Thompson, et al.  \"J Zoo Wildl Med.\"  50, \"Measuring fat content using computed tomography to establish a body condition index in free-ranging Blanding's turtles (Emydoidea blandingii) in Illinois.\" 594 (2019)","DOI":"10.1638\/2018-0154"},{"key":"25","doi-asserted-by":"crossref","unstructured":"CM McKenzie, ML Piczak, HN Snyman, et al.  \"Dis Aquat Organ.\"  132, \"First report of ranavirus mortality in a common snapping turtle Chelydra serpentina.\" 221 (2019)","DOI":"10.3354\/dao03324"},{"key":"1","doi-asserted-by":"crossref","unstructured":"CM Aiello, KE Nussear, TC Esque, et al.  \"J Anim Ecol.\"  85, \"Host contact and shedding patterns clarify variation in pathogen exposure and transmission in threatened tortoise Gopherus agassizii: Implications for disease modelling and management\" 829 (2016)","DOI":"10.1111\/1365-2656.12511"},{"key":"3","doi-asserted-by":"crossref","unstructured":"KE Andersson, L Adamovicz, LE Mumm, et al.  \"PLoS One.\"  16, \"Plasma electrophoresis profiles of Blanding's turtles (Emydoidea blandingii) and influences of month, age, sex, health status, and location.\" e0258397 (2021)","DOI":"10.1371\/journal.pone.0258397"},{"key":"6","doi-asserted-by":"crossref","unstructured":"FR. Cagle,  \"Ecol Monogr.\"  20, \"The life history of the slider turtle, Pseudemys scripta troostii (Holbrook).\" 31 (1950)","DOI":"10.2307\/1943522"},{"key":"7","unstructured":"V. Chongsuvivatwong,  \"epiDisplay: Epidemiological data display package. R version 3.5.0.1.\"  (2018)"},{"key":"12","doi-asserted-by":"crossref","unstructured":"MM Garner, R Herrington, EW Howerth, et al.  \"J Wildl Dis.\"  33, \"Shell disease in river cooters (Pseudemys concinna) and yellow-bellied turtles (Trachemys scripta) in a Georgia (USA) lake.\" 78 (1997)","DOI":"10.7589\/0090-3558-33.1.78"},{"key":"13","doi-asserted-by":"crossref","unstructured":"JW Gibbons, RD Semlitsch, JL Greene, et al.  \"Am Nat.\"  117, \"Variation in age and size at maturity of the slider turtle (Pseudemys scripta).\" 841 (1981)","DOI":"10.1086\/283774"},{"key":"15","doi-asserted-by":"crossref","unstructured":"CK Golba, GA Glowacki, RB King,  \"Ichthyol Herpetol.\"  110, \"Growth and survival of wild and head-started Blanding's turtles (Emydoidea blandingii).\" 378 (2022)","DOI":"10.1643\/h2021005"},{"key":"18","doi-asserted-by":"crossref","unstructured":"ER Jacobson  and F Origgi  \"Semin Avian Exot Pet Med.\"  11, \"Use of serology in reptile medicine.\" 33 (2002)","DOI":"10.1053\/saep.2002.28239"},{"key":"22","doi-asserted-by":"crossref","unstructured":"A Luzuriaga-Neira, FC Sandmeier, CL Weitzman, et al.  \"PLoS One.\"  16, \"Mycoplasma agassizii, an opportunistic pathogen of tortoises, shows very little genetic variation across the Mojave and Sonoran Deserts.\" e0245895 (2021)","DOI":"10.1371\/journal.pone.0245895"},{"key":"30","doi-asserted-by":"crossref","unstructured":"ZC Ready, L Adamovicz, M Daleo, et al.  \"J Virol Methods.\"  330, \"Development and validation of a quantitative PCR assay for detection of Sulawesi tortoise adenovirus.\" 115033 (2024)","DOI":"10.1016\/j.jviromet.2024.115033"},{"key":"31","doi-asserted-by":"crossref","unstructured":"ZC Ready, L Adamovicz, JFX Wellehan, et al.  \"J Wildl Dis.\"  61, \"Detection of adenoviruses in free-ranging Blanding's turtles (Emydoidea blandingii), painted turtles (Chrysemys picta), and red-eared sliders (Trachemys scripta elegans) in Illinois, USA.\" 370 (2025)","DOI":"10.7589\/JWD-D-24-00087"},{"key":"32","unstructured":"AGJ Rhodin, JB Iverson, R Bour, et al.  \"Conservation biology of freshwater turtles and tortoises: A compilation project of the IUCN\/SSC tortoise and freshwater turtle specialist group.\"  \"Turtles of the world: Annotated checklist and atlas of taxonomy, synonymy, distribution, and conservation status.\" 1 (2021)"},{"key":"33","doi-asserted-by":"crossref","unstructured":"LW. Riley,  \"Microbiol Spectr.\"  7, \"Differentiating epidemic from endemic or sporadic infectious disease occurrence.\" 10.1128 (2019)","DOI":"10.1128\/microbiolspec.AME-0007-2019"},{"key":"35","unstructured":"DA. Ross,  \"Wis Acad Sci Arts Lett.\"  77, \"Population ecology of painted and Blanding's turtles (Chrysemys picta and Emydoidea blandingi) in central Wisconsin.\" 77 (1989)"},{"key":"37","doi-asserted-by":"crossref","unstructured":"FC Sandmeier, CL Weitzman, CR Tracy,  \"Ecosphere.\"  9, \"An ecoimmunological approach to disease in tortoises reveals the importance of lymphocytes.\" e02427 (2018)","DOI":"10.1002\/ecs2.2427"},{"key":"40","doi-asserted-by":"crossref","unstructured":"GR Takuissu, S Kenmoe, JT Ebogo-Belobo, et al.  \"Int J Environ Health Res.\"  34, \"Exploring adenovirus in water environments: A systematic review and meta-analysis.\" 2504 (2024)","DOI":"10.1080\/09603123.2023.2255559"},{"key":"41","doi-asserted-by":"crossref","unstructured":"AV T\u00f3th, P Berta, B Harrach, et al.  \"Infect Genet Evol.\"  125, \"Discovery of the first sea turtle adenovirus and turtle associated circoviruses.\" 105677 (2024)","DOI":"10.1016\/j.meegid.2024.105677"},{"key":"42","unstructured":"N Usman  and M Suarez  \"StatPearls.\"  \"Adenoviruses.\" (2023)"},{"key":"19","doi-asserted-by":"publisher","unstructured":"LP Kane, MC Allender, G Archer, et al.  \"J Wildl Dis.\"  53, \"Prevalence of Terrapene herpesvirus 1 in free-ranging eastern box turtles (Terrapene carolina carolina) in Tennessee and Illinois, USA.\" 285 (2017)","DOI":"10.7589\/2016-06-138"},{"key":"38","doi-asserted-by":"crossref","unstructured":"VL Schumacher, CJ Innis, MM Garner, et al.  \"J Zoo Wildl Med.\"  43, \"Sulawesi tortoise adenovirus-1 in two impressed tortoises (Manouria impressa) and a Burmese star tortoise (Geochelone platynota).\" 501 (2012)","DOI":"10.1638\/2011-0228R.1"},{"key":"5","doi-asserted-by":"publisher","unstructured":"GA Archer, CA Phillips, L Adamovicz, et al.  \"J Zoo Wildl Med.\"  48, \"Detection of copathogens in free-ranging eastern box turtles (Terrapene carolina carolina) in Illinois and Tennessee.\" 1127 (2017)","DOI":"10.1638\/2017-0148R.1"},{"key":"23","doi-asserted-by":"crossref","unstructured":"RE. Marschang,  \"Viruses.\"  3, \"Viruses infecting reptiles.\" 2087 (2011)","DOI":"10.3390\/v3112087"},{"key":"8","doi-asserted-by":"publisher","unstructured":"A Doszpoly, JFX Wellehan, AL Childress, et al.  \"Infect Genet Evol.\"  17, \"Partial characterization of a new adenovirus lineage discovered in testudinoid turtles.\" 106 (2013)","DOI":"10.1016\/j.meegid.2013.03.049"},{"key":"14","doi-asserted-by":"crossref","unstructured":"PM Gibbons  and ZJ Steffes  \"Vet Clin North Am Exot Anim Pract.\"  16, \"Emerging infectious diseases of chelonians.\" 303 (2013)","DOI":"10.1016\/j.cvex.2013.02.004"},{"key":"34","doi-asserted-by":"crossref","unstructured":"S Rivera, JFX Wellehan, R McManamon, et al.  \"J Vet Diagn Invest.\"  21, \"Systemic adenovirus infection in Sulawesi tortoises (Indotestudo forsteni) caused by a novel siadenovirus.\" 415 (2009)","DOI":"10.1177\/104063870902100402"},{"key":"17","doi-asserted-by":"crossref","unstructured":"K Hardman, A Dastjerdi, R Gurrala, et al.  \"Vet Rec.\"  170, \"Detection of elephant endotheliotropic herpesvirus type 1 in asymptomatic elephants using TaqMan real-time PCR.\" 205 (2012)","DOI":"10.1136\/vr.100270"},{"key":"11","doi-asserted-by":"crossref","unstructured":"B Garcia-Morante, JJ P\u00e9nzes, T Costa, et al.  \"J Vet Diagn Invest.\"  28, \"Hyperplastic stomatitis and esophagitis in a tortoise (Testudo graeca) associated with an adenovirus infection.\" 579 (2016)","DOI":"10.1177\/1040638716659903"},{"key":"10","doi-asserted-by":"crossref","unstructured":"D Franzen-Klein, L Adamovicz, D McRuer, et al.  \"J Zoo Wildl Med.\"  50, \"Prevalence of box turtle adenovirus in eastern box turtles (Terrapene carolina carolina) presented to a wildlife rehabilitation center in Virginia, USA.\" 769 (2020)","DOI":"10.1638\/2018-0238"},{"key":"28","doi-asserted-by":"crossref","unstructured":"L Ogorzaly, I Bertrand, M Paris, et al.  \"Appl Environ Microbiol.\"  76, \"Occurrence, survival, and persistence of human adenoviruses and F-specific RNA phages in raw groundwater.\" 8019 (2010)","DOI":"10.1128\/AEM.00917-10"},{"key":"44","doi-asserted-by":"crossref","unstructured":"EB. Wilson,  \"J Am Stat Assoc.\"  22, \"Probable inference, the law of succession, and statistical inference.\" 209 (1927)","DOI":"10.1080\/01621459.1927.10502953"},{"key":"24","unstructured":"MJ. Mazerolle,  \"AICcmodavg: model selection and multimodel inference based on (Q)AIC(c). R package version 2.1-1.\"  (2017)"},{"key":"29","unstructured":"\"R: a language and environment for statistical computing.\"  (2024)"},{"key":"20","doi-asserted-by":"crossref","unstructured":"DM Lindemann, MC Allender, D Thompson, et al.  \"J Virol Methods.\"  254, \"Development and validation of a quantitative PCR assay for detection of Emydoidea herpesvirus 1 in free-ranging Blanding's turtles (Emydoidea blandingii)\" 40 (2018)","DOI":"10.1016\/j.jviromet.2018.01.006"},{"key":"9","doi-asserted-by":"crossref","unstructured":"CH Ernst  and JE Lovich  \"Turtles of the United States and Canada.\"  827 (2009)","DOI":"10.56021\/9780801891212"},{"key":"4","doi-asserted-by":"crossref","unstructured":"KE Andersson, L Adamovicz, LE Mumm, et al.  \"J Vet Diagn Invest.\"  33, \"Detection of a novel herpesvirus associated with squamous cell carcinoma in a free-ranging Blanding's turtle.\" 348 (2021)","DOI":"10.1177\/1040638721989302"},{"key":"16","unstructured":"\"GraphPad Prism, version 9.\"  (2023)"},{"key":"39","doi-asserted-by":"crossref","unstructured":"R St. Clair, PT Gregory, JM Macartney,  \"Can J Zool.\"  72, \"How do sexual differences in growth and maturation interact to determine size in northern and southern painted turtles?\" 1436 (1994)","DOI":"10.1139\/z94-190"},{"key":"43","doi-asserted-by":"crossref","unstructured":"EC Vincent, MA Fayette, JA Griffioen, et al.  \"J Wildl Dis.\"  59, \"Health assessment of painted turtles (Chrysemys picta) in a restored wetland habitat in northwestern Indiana, USA.\" 245 (2023)","DOI":"10.7589\/JWD-D-22-00088"},{"key":"21","doi-asserted-by":"crossref","unstructured":"DM Lindemann, MC Allender, D Thompson, et al.  \"J Zoo Wildl Med.\"  50, \"Epidemiology of Emydoidea herpesvirus 1 in free-ranging Blanding's turtles (Emydoidea blandingii) from Illinois.\" 547 (2019)","DOI":"10.1638\/2018-0074"},{"key":"26","doi-asserted-by":"crossref","unstructured":"LE Mumm, JM Winter, KE Andersson, et al.  \"PLoS One.\"  14, \"Hematology and plasma biochemistries in the Blanding's turtle (Emydoidea blandingii) in Lake County, Illinois.\" e0225130 (2019)","DOI":"10.1371\/journal.pone.0225130"},{"key":"36","doi-asserted-by":"crossref","unstructured":"E Salzmann, E M\u00fcller, RE Marschang,  \"J Zoo Wildl Med.\"  52, \"Detection of testadenoviruses and atadenoviruses in tortoises and turtles in Europe.\" 223 (2021)","DOI":"10.1638\/2020-0078"},{"key":"45","doi-asserted-by":"crossref","unstructured":"JM Winter, L Mumm, LA Adamovicz, et al.  \"J Zoo Wildl Med.\"  51, \"Characterizing the epidemiology of historic and novel pathogens in Blanding's turtles (Emydoidea blandingii).\" 606 (2020)","DOI":"10.1638\/2019-0154"}],"container-title":["Journal of Wildlife Diseases"],"original-title":[],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:17:14Z","timestamp":1791242234000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/journal-of-wildlife-diseases\/volume-61\/issue-2\/JWD-D-24-00124\/EPIDEMIOLOGY-OF-SULAWESI-TORTOISE-ADENOVIRUS-IN-FREE-LIVING-BLANDINGS-TURTLES\/10.7589\/JWD-D-24-00124.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,16]]},"references-count":45,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,4,1]]}},"URL":"https:\/\/doi.org\/10.7589\/jwd-d-24-00124","relation":{},"ISSN":["0090-3558","1943-3700"],"issn-type":[{"value":"0090-3558","type":"print"},{"value":"1943-3700","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,16]]}}}