{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:52:19Z","timestamp":1791244339349,"version":"4.1.0"},"reference-count":19,"publisher":"Wildlife Disease Association","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Wildlife Diseases"],"published-print":{"date-parts":[[2025,7,11]]},"abstract":"<jats:p>Neospora caninum is an obligate intracellular protozoan parasite that causes hind-limb paralysis in dogs and abortions in cattle. Recurrent storms of abortions in cattle in Australia are estimated to cause an economic loss of AU $100 million each year, but the source of infection in cattle (i.e., via wild or domestic definitive hosts) remains to be established. We aimed to determine potential sources of horizontal transmission of N. caninum after recent evidence that wild dogs (Canis familiaris) shed N. caninum oocysts in Victoria, Australia. To determine infection levels among wild dogs, samples were collected from three sites in Victoria (n=52) in areas of high wild dog population density (Mansfield, Swifts Creek, and Tallangatta), and N. caninum detection was determined by PCR analysis of blood and duodenal samples. Based on the PCR assay of combined duodenal samples (flotation of duodenal content and just duodenal content), this study detected N. caninum in the fecal material of 11% of wild dogs in Mansfield and 21% of wild dogs in Swifts Creek. This study provides further insight into the possible role of wild dogs as a reservoir of N. caninum in Victoria.<\/jats:p>","DOI":"10.7589\/jwd-d-24-00149","type":"journal-article","created":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T08:54:31Z","timestamp":1752742471000},"page":"714-718","source":"Crossref","is-referenced-by-count":0,"title":["Moderate Neospora caninum Detection in Wild Dog Populations in Southeast Australia"],"prefix":"10.7589","volume":"61","author":[{"given":"Eliza K.","family":"Stott","sequence":"first","affiliation":[{"name":"One Health Research Group, Melbourne Veterinary School, Faculty of Science, Werribee, The University of Melbourne, Werribee, 3030, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose L.","family":"Huaman","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Anatomy, Physiology, and Pharmacology, School of Agriculture, Biomedicine and Environment, La Trobe University, Melbourne, 3000, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa G.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Anatomy, Physiology, and Pharmacology, School of Agriculture, Biomedicine and Environment, La Trobe University, Melbourne, 3000, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"McCowan","sequence":"additional","affiliation":[{"name":"One Health Research Group, Melbourne Veterinary School, Faculty of Science, Werribee, The University of Melbourne, Werribee, 3030, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chloe J.","family":"Fingland","sequence":"additional","affiliation":[{"name":"Delatite Veterinary Services, 265 Mt. Buller Road, Mansfield, 3722, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jade F.","family":"Hammer","sequence":"additional","affiliation":[{"name":"Main Street Veterinary Clinic, Bairnsdale, 3875, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alana","family":"Websdale","sequence":"additional","affiliation":[{"name":"Main Street Veterinary Clinic, Bairnsdale, 3875, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee F.","family":"Skerratt","sequence":"additional","affiliation":[{"name":"One Health Research Group, Melbourne Veterinary School, Faculty of Science, Werribee, The University of Melbourne, Werribee, 3030, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5115-4120","authenticated-orcid":false,"given":"Carlo","family":"Pacioni","sequence":"additional","affiliation":[{"name":"Department of Environment, Land, Water and Planning, Arthur Rylah Institute for Environmental Research, Heidelberg, 3084, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4415","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"DJD Banks, DB Copeman, LF Skerratt,  \"Aust Vet J.\"  84, \"Echinococcus granulosus in northern Queensland. 2. Ecological determinants of infection in beef cattle.\" 308 (2006)","DOI":"10.1111\/j.1751-0813.2006.00021.x"},{"key":"2","doi-asserted-by":"crossref","unstructured":"E Collantes-Fern\u00e1ndez, M G\u00f3mez-Bautista, G Mir\u00f3, et al.  \"Vet Parasitol.\"  152, \"Seroprevalence and risk factors associated with Neospora caninum infection in different dog populations in Spain.\" 148 (2008)","DOI":"10.1016\/j.vetpar.2007.12.005"},{"key":"3","doi-asserted-by":"crossref","unstructured":"MJ Davidson, JL Huaman, C Pacioni, et al.  \"Transbound Emerg Dis.\"  69, \"Active shedding of Neospora caninum detected in Australian wild canids in a non-experimental context.\" 1862 (2021)","DOI":"10.1111\/tbed.14170"},{"key":"4","doi-asserted-by":"crossref","unstructured":"K Dilley, F Pagan, B Chapman,  \"Sci Justice.\"  61, \"Methods for ensuring the highest DNA concentration and yield in future and retrospective trace DNA extracts.\" 193 (2021)","DOI":"10.1016\/j.scijus.2020.11.005"},{"key":"5","unstructured":"S. Dorai-Raj,  \"R package'Binom'.\"  \"Binomial confidence intervals for several parameterizations\" (2014)"},{"key":"6","doi-asserted-by":"crossref","unstructured":"JP. Dubey,  \"J Parasitol.\"  81, \"Duration of immunity to shedding of Toxoplasma gondii oocysts by cats.\" 410 (1995)","DOI":"10.2307\/3283823"},{"key":"7","doi-asserted-by":"crossref","unstructured":"JP Dubey, G Schares, LM Ortega-Mora,  \"Clin Microbiol Rev.\"  20, \"Epidemiology and control of neosporosis and Neospora caninum.\" 323 (2007)","DOI":"10.1128\/CMR.00031-06"},{"key":"8","doi-asserted-by":"crossref","unstructured":"NP French, D Clancy, HC Davison, et al.  \"Int J Parasitol.\"  29, \"Mathematical models of Neospora caninum infection in dairy cattle: Transmission and options for control.\" 1691 (1999)","DOI":"10.1016\/S0020-7519(99)00131-9"},{"key":"9","doi-asserted-by":"crossref","unstructured":"AS Glen, CR Dickman, ME Soule, et al.  \"Austral Ecol.\"  32, \"Evaluating the role of the dingo as a trophic regulator in Australian ecosystems.\" 492 (2007)","DOI":"10.1111\/j.1442-9993.2007.01721.x"},{"key":"10","doi-asserted-by":"crossref","unstructured":"JS King, DJ Jenkins, JT Ellis, et al.  \"Vet J.\"  188, \"Implications of wild dog ecology on the sylvatic and domestic life cycle of Neospora caninum in Australia.\" 24 (2011a)","DOI":"10.1016\/j.tvjl.2010.03.002"},{"key":"11","doi-asserted-by":"crossref","unstructured":"JS King, B McAllan, DS Spielman, et al.  \"Vet Res.\"  42, \"Extensive production of Neospora caninum tissue cysts in a carnivorous marsupial succumbing to experimental neosporosis.\" 75 (2011b)","DOI":"10.1186\/1297-9716-42-75"},{"key":"12","doi-asserted-by":"crossref","unstructured":"JS King, J \u0160lapeta, DJ Jenkins, et al.  \"Int J Parasitol.\"  40, \"Australian dingoes are definitive hosts of Neospora caninum.\" 945 (2010)","DOI":"10.1016\/j.ijpara.2010.01.008"},{"key":"13","unstructured":"MM McAllister, J Dubey, DS Lindsay, et al.  \"Int J Parasitol.\"  28, \"Dogs are definitive hosts of Neospora caninum.\" 1473 (1998)"},{"key":"15","doi-asserted-by":"crossref","unstructured":"MP. Reichel,  \"Aust Vet J.\"  78, \"Neospora caninum infections in Australia and New Zealand.\" 258 (2000)","DOI":"10.1111\/j.1751-0813.2000.tb11751.x"},{"key":"16","doi-asserted-by":"crossref","unstructured":"AC Rosypal  and DS Lindsay  \"Trends Parasitol.\"  21, \"The sylvatic cycle of Neospora caninum: Where do we go from here?\" 439 (2005)","DOI":"10.1016\/j.pt.2005.08.003"},{"key":"17","doi-asserted-by":"crossref","unstructured":"D Stephens, AN Wilton, PJ Fleming, et al.  \"Mol Ecol.\"  24, \"Death by sex in an Australian icon: A continent-wide survey reveals extensive hybridization between dingoes and domestic dogs.\" 5643 (2015)","DOI":"10.1111\/mec.13416"},{"key":"18","doi-asserted-by":"crossref","unstructured":"W Wouda, T Dijkstra, AMH Kramer, et al.  \"Int J Parasitol.\"  29, \"Seroepidemiological evidence for a relationship between Neospora caninum infections in dogs and cattle.\" 1677 (1999)","DOI":"10.1016\/S0020-7519(99)00105-8"},{"key":"19","doi-asserted-by":"crossref","unstructured":"H Yamashita, T Kazawa, Y Minatogawa, et al.  \"Int J Neuropsychopharmacol.\"  5, \"Time-course of hepatic cytochrome P450 subfamily induction by chronic carbamazepine treatment in rats.\" 47 (2002)","DOI":"10.1017\/S1461145701002747"},{"key":"14","unstructured":"\"R: a language and environment for statistical computing.\"  (2024)"}],"container-title":["Journal of Wildlife Diseases"],"original-title":[],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T22:59:25Z","timestamp":1791241165000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/journal-of-wildlife-diseases\/volume-61\/issue-3\/JWD-D-24-00149\/Moderate-Neospora-caninum-Detection-in-Wild-Dog-Populations-in-Southeast\/10.7589\/JWD-D-24-00149.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,11]]},"references-count":19,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,7,1]]}},"URL":"https:\/\/doi.org\/10.7589\/jwd-d-24-00149","relation":{},"ISSN":["0090-3558","1943-3700"],"issn-type":[{"value":"0090-3558","type":"print"},{"value":"1943-3700","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,11]]}}}