{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:54:14Z","timestamp":1791244454239,"version":"4.1.0"},"reference-count":33,"publisher":"Wildlife Disease Association","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Wildlife Diseases"],"published-print":{"date-parts":[[2025,5,16]]},"abstract":"<jats:p>Between November 2021 and January 2023, the eyes of either slaughtered or deceased male American bison (Bison bison) and European bison (Bison bonasus) from Romania were investigated for the presence of Thelazia spp. nematodes (eye worms). These included seven farmed American bison from two locations and two wild European bison from reintroduction sites. Thelazia rhodesi and Thelazia skrjabini were present in three American bison and both European bison. A total of 38 nematodes were recovered, with T. rhodesi being the predominant species. Molecular analysis confirmed these findings and identified unique sequences for both species. The nematodes in American bison were distributed across the Pannonian and Continental biogeoregions, and those in European bison originated from the Alpine region. Thelazia spp. have not previously been reported in American bison, nor in European bison from Romania. The findings underscore the need for continuous monitoring and management of Thelazia infections to support conservation and reintroduction efforts. Future research should focus on the ecological factors influencing these infections and develop strategies to mitigate their impact on wildlife health.<\/jats:p>","DOI":"10.7589\/jwd-d-24-00169","type":"journal-article","created":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T00:33:02Z","timestamp":1747355582000},"page":"536-541","source":"Crossref","is-referenced-by-count":1,"title":["Thelazia rhodesi and Thelazia skrjabini in Wild European Bison (Bison bonasus) and Farmed American Bison (Bison bison) from Romania, 2021\u201323"],"prefix":"10.7589","volume":"61","author":[{"given":"Vlad-Dan","family":"Cotu\u0163iu","sequence":"first","affiliation":[{"name":"Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Calea M\u0103n\u0103\u015ftur 3-5, Cluj-Napoca, Cluj County 400372, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina D.","family":"Cazan","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Calea M\u0103n\u0103\u015ftur 3-5, Cluj-Napoca, Cluj County 400372, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela M.","family":"Ionic\u0103","sequence":"additional","affiliation":[{"name":"Clinical Hospital of Infectious Diseases of Cluj-Napoca, Iuliu Moldovan Street Nr. 23, Cluj-Napoca, Cluj County 400003, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrada S.","family":"C\u00e2rstolovean","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Calea M\u0103n\u0103\u015ftur 3-5, Cluj-Napoca, Cluj County 400372, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angel-Gabriel","family":"Irimia","sequence":"additional","affiliation":[{"name":"University of Bucharest, Faculty of Biology, Splaiul Independen\u0163ei 91\u201395, Bucharest 050095, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian M.","family":"Aldea","sequence":"additional","affiliation":[{"name":"Foundation Conservation Carpathia, Calea Feldioarei 27A, Bra\u015fov, Bra\u015fov County 500450, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u0103lin C.","family":"\u015eerban","sequence":"additional","affiliation":[{"name":"Foundation Conservation Carpathia, Calea Feldioarei 27A, Bra\u015fov, Bra\u015fov County 500450, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel B.","family":"Chi\u015famera","sequence":"additional","affiliation":[{"name":"Institute of Biology Bucharest, Romanian Academy, Splaiul Independen\u0163ei 296, Bucharest 060031, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina D.","family":"Ha\u015fa\u015f","sequence":"additional","affiliation":[{"name":"Department of Physiopathology, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Calea M\u0103n\u0103\u015ftur 3-5, Cluj-Napoca, Cluj County 400372, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrei D.","family":"Mihalca","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Calea M\u0103n\u0103\u015ftur 3-5, Cluj-Napoca, Cluj County 400372, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4415","reference":[{"key":"1","unstructured":""},{"key":"12","doi-asserted-by":"publisher","unstructured":"\"GBIF Backbone Taxonomy. Checklist dataset.\"  (2023)","DOI":"10.15468\/39omei"},{"key":"2","unstructured":"IK. Barker,  \"Can Vet J.\"  11, \"Case report. Thelazia lacrymalis from the eyes of an Ontario horse.\" 186 (1970)"},{"key":"3","doi-asserted-by":"crossref","unstructured":"RS Bradbury, KV Breen, EM Bonura, et al.  \"Am J Trop Med Hyg.\"  98, \"Case report: Conjunctival infestation with Thelazia gulosa: A novel agent of human thelaziasis in the United States.\" 1171 (2018)","DOI":"10.4269\/ajtmh.17-0870"},{"key":"4","doi-asserted-by":"crossref","unstructured":"JF Burger  and JR Anderson  \"Ann Entomol Soc Am.\"  63, \"Association of the face fly, Musca autumnalis, with bison in western North America.\" 635 (1970)","DOI":"10.1093\/aesa\/63.3.635"},{"key":"6","doi-asserted-by":"crossref","unstructured":"V-D Cotutiu, AM Ionic\u0103, T Dan, et al.  \"Parasit Vectors.\"  16, \"Diversity of Thelazia spp. in domestic cattle from Romania: Epidemiology and molecular diagnosis by a novel multiplex PCR.\" 400 (2023)","DOI":"10.1186\/s13071-023-06012-8"},{"key":"7","doi-asserted-by":"crossref","unstructured":"V-D Cotutiu, AM Ionic\u0103, M Lefkaditis, et al.  \"Parasit Vectors.\"  15, \"Thelazia lacrymalis in horses from Romania: Epidemiology, morphology and phylogenetic analysis.\" 425 (2022)","DOI":"10.1186\/s13071-022-05532-z"},{"key":"8","doi-asserted-by":"crossref","unstructured":"AW Demiaszkiewicz, B Moskwa, A Gralak, et al.  \"Acta Parasitol.\"  65, \"The nematodes Thelazia gulosa Railiet and Henry, 1910 and Thelazia skrjabini Erschov, 1928 as a cause of blindness in European bison (Bison bonasus) in Poland.\" 963 (2020)","DOI":"10.1007\/s11686-020-00243-w"},{"key":"9","unstructured":"N. Dulceanu,  \"Cercet\u0103ri etiologice \u015fi epizootologice \u00een telazioza taurinelor din judet,ul Ia\u015fi.\"  (1971)"},{"key":"10","doi-asserted-by":"crossref","unstructured":"N Ercan, A Yildirim, O Duzlu, et al.  \"Med Vet Entomol.\"  38, \"Identification and distribution of some medico-veterinary important pathogens in muscid flies in two geographical regions of T\u00fcrkiye.\" 440 (2024)","DOI":"10.1111\/mve.12734"},{"key":"11","doi-asserted-by":"crossref","unstructured":"K Filip-Hutsch, AW Demiaskiewicz, T Hutsch, et al.  \"BMC Vet Res.\"  20, \"Infectious keratoconjunctivitis in European bison (Bison bonasus) in Poland: Risk factors, epidemiology and anatomopathological changes with analysis of potential role of Thelazia nematodes in the disease development.\" 524 (2024)","DOI":"10.1186\/s12917-024-04375-3"},{"key":"13","doi-asserted-by":"crossref","unstructured":"AC. Isenberg,  \"The destruction of the bison: An environmental history, 1750\u20131920.\"  (2001)","DOI":"10.1017\/CBO9780511549861"},{"key":"14","doi-asserted-by":"crossref","unstructured":"G Karbowiak, AW Demiaszkiewicz, AM Pyziel, et al.  \"Acta Parasitol.\"  59, \"The parasitic fauna of the European bison (Bison bonasus) (Linnaeus, 1758) and their impact on the conservation. Part 1. The summarizing list of parasites noted.\" 363 (2014)","DOI":"10.2478\/s11686-014-0252-0"},{"key":"15","unstructured":"A Kashtalian, A Subbotin, T Shendrik,  \"Eur Bison Conserv Newsletter.\"  6, \"Helminthofauna of European bison from Borisov-Berezinsky free-living population.\" 95 (2013)"},{"key":"16","doi-asserted-by":"crossref","unstructured":"MJ Kennedy, DT Moraiko, L Goonewardene,  \"J Parasitol.\"  76, \"A study on the prevalence and intensity of occurrence of Thelazia skrjabini (Nematoda: Thelazioidea) in cattle in Central Alberta, Canada.\" 196 (1990)","DOI":"10.2307\/3283015"},{"key":"17","doi-asserted-by":"crossref","unstructured":"D Klich, A Didkowska, AM Pyziel-Serafin, et al.  \"PloS One.\"  18, \"Contact between European bison and cattle from the cattle breeders' perspective, in the light of the risk of pathogen transmission.\" e0285245 (2023)","DOI":"10.1371\/journal.pone.0285245"},{"key":"18","doi-asserted-by":"crossref","unstructured":"ES Krafsur  and RD Moon  \"Annu Rev Entomol.\"  42, \"Bionomics of the face fly, Musca autumnalis.\" 503 (1997)","DOI":"10.1146\/annurev.ento.42.1.503"},{"key":"19","doi-asserted-by":"crossref","unstructured":"M Krasi\u0144ska  and ZA Krasi\u0144ski  \"European bison. The nature monograph.\"  (2013)","DOI":"10.1007\/978-3-642-36555-3"},{"key":"20","unstructured":"T Kuzmina, NS Zvegintsova, K Slivinska, et al.  \"Bull Biosphere Reserve'Askania-Nova'.\"  21, \"Helminths of ungulates included into the red book of Ukraine.\" 213 (2019)"},{"key":"21","unstructured":"D Mot  and T Mot  \"Agric Banatului.\"  4, \"Ectoparazitismul cu muscide la animalele de rent\u0103.\" 22 (2019)"},{"key":"23","unstructured":"I Nichita, M Decun, I tibru, et al.  \"Ia\u015fi.\"  43, \"Muscidae species from pig and cattle shelters. Lucr\u0103i Stiinifice\u2013Medicin\u0103 Veterinar\u0103, Universitatea de Stiinte Agricole \u015fi Medicin\u0103 Veterinar\u0103 \u201cIon Ionescu de la Brad\" 233 (2000)"},{"key":"24","doi-asserted-by":"crossref","unstructured":"D Otranto  and P Deplazes  \"Int J Parasitol Parasites Wildl.\"  9, \"Zoonotic nematodes of wild carnivores.\" 370 (2019)","DOI":"10.1016\/j.ijppaw.2018.12.011"},{"key":"25","doi-asserted-by":"crossref","unstructured":"D Otranto, JA Mendoza-Roldan, F Dantas-Torres,  \"Trends Parasitol.\"  37, \"Thelazia callipaeda.\" 263 (2021)","DOI":"10.1016\/j.pt.2020.04.013"},{"key":"26","doi-asserted-by":"crossref","unstructured":"D Otranto, E Tarsitano, D Traversa, et al.  \"Int J Parasitol.\"  31, \"Differentiation among three species of bovine Thelazia (Nematoda: Thelaziidae) by polymerase chain reaction\u2013restriction fragment length polymorphism of the first internal transcribed spacerITS-1 (rDNA).\" 1693 (2001)","DOI":"10.1016\/S0020-7519(01)00279-X"},{"key":"27","doi-asserted-by":"crossref","unstructured":"D Otranto  and D Traversa  \"Trends Parasitol.\"  21, \"Thelazia eyeworm: An original endo- and ecto-parasitic nematode.\" 1 (2005)","DOI":"10.1016\/j.pt.2004.10.008"},{"key":"28","doi-asserted-by":"crossref","unstructured":"EW Sanderson, KH. Redford, B Weber, et al.  \"Conserv Biol.\"  22, \"The ecological future of the North American bison: Conceiving long-term, large-scale conservation of wildlife.\" 252 (2008)","DOI":"10.1111\/j.1523-1739.2008.00899.x"},{"key":"29","unstructured":"KI Skrjabin, AA Soboley, VM Ivanshkin,  \"Essentials of nematodology.\"  XVI, \"Spirurata of animals and man and the diseases caused by them Part 4: Thelazioidea.\" 1 (1967)"},{"key":"30","doi-asserted-by":"crossref","unstructured":"C Sobotyk, T Foster, RT Callahan, et al.  \"Vet Parasitol Reg Stud Reports.\"  24, \"Zoonotic Thelazia californiensis in dogs from New Mexico, USA, and a review of North American cases in animals and humans.\" 100553 (2021)","DOI":"10.1016\/j.vprsr.2021.100553"},{"key":"31","unstructured":"SV. Tessaro,  \"Can Vet J.\"  30, \"Review of the diseases, parasites and miscellaneous pathological conditions of North American bison.\" 416 (1989)"},{"key":"32","unstructured":"J. van de Vlasakker,  \"Bison rewilding plan, 2014\u20132024. Rewilding Europe.\"  (2014)"},{"key":"33","unstructured":"\"Zimbrul.\"  (2023)"},{"key":"5","doi-asserted-by":"crossref","unstructured":"M Casiraghi, TJ Anderson, C Bandi, et al.  \"Parasitology.\"  122, \"A phylogenetic analysis of filarial nematodes: comparison with the phylogeny of Wolbachia endosymbionts.\" 93 (2001)","DOI":"10.1017\/S0031182000007149"},{"key":"22","unstructured":"\"Basic local alignment search tool (BLAST).\"  (2023)"}],"container-title":["Journal of Wildlife Diseases"],"original-title":[],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:17:28Z","timestamp":1791242248000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/journal-of-wildlife-diseases\/volume-61\/issue-2\/JWD-D-24-00169\/Thelazia-rhodesi-and-Thelazia-skrjabini-in-Wild-European-Bison-Bison\/10.7589\/JWD-D-24-00169.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,16]]},"references-count":33,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,4,1]]}},"URL":"https:\/\/doi.org\/10.7589\/jwd-d-24-00169","relation":{},"ISSN":["0090-3558","1943-3700"],"issn-type":[{"value":"0090-3558","type":"print"},{"value":"1943-3700","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,16]]}}}