{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:58:45Z","timestamp":1791237525968,"version":"4.1.0"},"reference-count":46,"publisher":"Wildlife Disease Association","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Wildlife Diseases"],"published-print":{"date-parts":[[2026,2,16]]},"abstract":"<jats:p>Gastric spirilliform bacteria consistent with Helicobacter spp. were identified in four pygmy sperm whales (Kogia breviceps) that stranded along the east coast of Florida, USA, during 2017\u201320. Six of nine (67%) stomach samples from PCR-positive cases contained spirilliform bacteria visible on histopathologic examination. Experimental PCR targeting Helicobacter-specific 16S rRNA gene sequences produced amplification in at least one gastric tissue sample (e.g., forestomach, fundus, pylorus) from all four cases. Three different novel Helicobacter sequences were identified in 13 samples from four whales. Helicobacter-positive samples could be classified into three novel sequences, Kogia Helicobacter 1, 2, and 3, represented by a common 572-base-pair region. All four whales in this study were infested with gastric nematodes and had one or more of the following histopathologic findings: gastritis, gastric ulceration, and gastric fibrosis. These lesions were not attributed as a cause of death in the whales but may have contributed to stranding via reduced fitness. This study reports three novel Helicobacter spp. bacteria identified in K. breviceps and expands the known host range of this genus. Further studies into these novel Helicobacter spp. should include additional pathologic and molecular characterization of the bacteria and any associated lesions.<\/jats:p>","DOI":"10.7589\/jwd-d-24-00204","type":"journal-article","created":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T09:38:13Z","timestamp":1771234693000},"page":"26-36","source":"Crossref","is-referenced-by-count":0,"title":["NOVEL GASTRIC HELICOBACTER SPECIES IN STRANDED PYGMY SPERM WHALES (KOGIA BREVICEPS) ON THE EAST COAST OF FLORIDA, USA"],"prefix":"10.7589","volume":"62","author":[{"given":"Wendy","family":"Marks","sequence":"first","affiliation":[{"name":"Florida Atlantic University, Harbor Branch Oceanographic Institute, 5600 US 1 North, Fort Pierce, Florida 34946, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessy","family":"Castellanos-Gell","sequence":"additional","affiliation":[{"name":"Department of Comparative, Diagnostic, and Population Medicine, College of Veterinary Medicine, University of Florida, 1945 SW 16th Avenue, Gainesville, Florida 32608, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven B.","family":"Tillis","sequence":"additional","affiliation":[{"name":"Department of Comparative, Diagnostic, and Population Medicine, College of Veterinary Medicine, University of Florida, 1945 SW 16th Avenue, Gainesville, Florida 32608, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Pegg","sequence":"additional","affiliation":[{"name":"Florida Atlantic University, Harbor Branch Oceanographic Institute, 5600 US 1 North, Fort Pierce, Florida 34946, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Rotstein","sequence":"additional","affiliation":[{"name":"Marine Mammal Pathology Services, 19117 Bloomfield Road, Olney, Maryland 20832, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sushan","family":"Han","sequence":"additional","affiliation":[{"name":"Colorado State University, Veterinary Diagnostics Lab, 2450 Gillette Drive, Fort Collins, Colorado 80526, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James F. X.","family":"Wellehan","sequence":"additional","affiliation":[{"name":"Department of Comparative, Diagnostic, and Population Medicine, College of Veterinary Medicine, University of Florida, 1945 SW 16th Avenue, Gainesville, Florida 32608, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"April","family":"Childress","sequence":"additional","affiliation":[{"name":"Department of Comparative, Diagnostic, and Population Medicine, College of Veterinary Medicine, University of Florida, 1945 SW 16th Avenue, Gainesville, Florida 32608, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve","family":"Burton","sequence":"additional","affiliation":[{"name":"Florida Atlantic University, Harbor Branch Oceanographic Institute, 5600 US 1 North, Fort Pierce, Florida 34946, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annie","family":"Page","sequence":"additional","affiliation":[{"name":"Florida Atlantic University, Harbor Branch Oceanographic Institute, 5600 US 1 North, Fort Pierce, Florida 34946, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4415","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"SE Balik, RJ Ossiboff, NI Stacy, et al.  \"Front Vet Sci.\"  10, \"Case report: Sarcocystis speeri, Aspergillus fumigatus, and novel Treponema sp. infections in an adult Atlantic spotted dolphin (Stenella frontalis).\" 1132161 (2023)","DOI":"10.3389\/fvets.2023.1132161"},{"key":"2","doi-asserted-by":"crossref","unstructured":"\"Geneious Prime version 2025.1.3.\"  (2025)","DOI":"10.2307\/jj.21874087.3"},{"key":"3","doi-asserted-by":"crossref","unstructured":"M Carbone, TL Maugeri, C Gugliandolo, et al.  \"J Appl Microbiol.\"  98, \"Occurrence of Helicobacter pylori DNA in the coastal environment of southern Italy (Straits of Messina).\" 768 (2005)","DOI":"10.1111\/j.1365-2672.2004.02517.x"},{"key":"4","doi-asserted-by":"crossref","unstructured":"L Cellini, A Del Vecchio, M Di Candia, et al.  \"J Appl Microbiol.\"  97, \"Detection of free and plankton-associated Helicobacter pylori in seawater.\" 285 (2004)","DOI":"10.1111\/j.1365-2672.2004.02307.x"},{"key":"5","doi-asserted-by":"crossref","unstructured":"P Cipriani, M Palomba, L Giulietti, et al.  \"Parasite.\"  31, \"Anisakid parasite diversity in a pygmy sperm whale Kogia breviceps (Cetacea: Kogiidae) stranded at the edge of its distribution range in the Northwest Atlantic Ocean.\" 43 (2024)","DOI":"10.1051\/parasite\/2024042"},{"key":"6","doi-asserted-by":"crossref","unstructured":"NJ Davison, JEF Barnett, M Koylass, et al.  \"J Wildl Dis.\"  50, \"Helicobacter cetorum infection in striped dolphin (Stenella coeruleoalba), Atlantic white-sided dolphin (Lagenorhynchus acutus), and short-beaked common dolphin (Delphinus delphus) from the southwest coast of England.\" 431 (2014)","DOI":"10.7589\/2013-02-047"},{"key":"7","doi-asserted-by":"crossref","unstructured":"S De Guise, A Lagace, P Beland, et al.  \"J Comp Pathol.\"  112, \"Non-neoplastic lesions in beluga whales (Delphinapterus leucas) and other marine mammals from the St. Lawrence Estuary.\" 257 (1995)","DOI":"10.1016\/S0021-9975(05)80079-9"},{"key":"8","doi-asserted-by":"crossref","unstructured":"M Duan, Y Li, J Liu, et al.  \"Helicobacter.\"  28, \"Transmission routes and patterns of Helicobacter pylori.\" e12945 (2023)","DOI":"10.1111\/hel.12945"},{"key":"9","unstructured":"PA Fair, JD Adams, E Zolman, et al.  \"NOAA Technical Memorandum NOS NCCOS.\"  49, \"Protocols for conducting dolphin capture-release health assessment studies.\" (2006)"},{"key":"10","doi-asserted-by":"crossref","unstructured":"Z Ge, Y Feng, S Muthupalani, et al.  \"Infect Immun.\"  79, \"Coinfection with enterohepatic Helicobacter species can ameliorate or promote Helicobacter pylori-induced gastric pathology in C57BL\/6 mice.\" 3861 (2011)","DOI":"10.1128\/IAI.05357-11"},{"key":"11","unstructured":"JR Geraci  and VJ Lounsbury  \"Marine mammals ashore: A field guide for strandings.\"  80 (2005)"},{"key":"12","doi-asserted-by":"crossref","unstructured":"CG Goldman, MJ Matteo, JD Loureiro, et al.  \"Vet Microbiol.\"  152, \"Novel gastric helicobacters and oral campylobacters are present in captive and wild cetaceans.\" 138 (2011)","DOI":"10.1016\/j.vetmic.2011.04.023"},{"key":"13","doi-asserted-by":"crossref","unstructured":"CG Goldman, MJ Matteo, JD Louriero, et al.  \"Vet Microbiol.\"  133, \"Detection of Helicobacter and Campylobacter spp. from the aquatic environment of marine mammals.\" 287 (2009)","DOI":"10.1016\/j.vetmic.2008.06.023"},{"key":"14","doi-asserted-by":"crossref","unstructured":"CG Harper, Y Feng, S Xu, et al.  \"J Clin Microbiol.\"  40, \"Helicobacter cetorum sp. nov., a ureasepositive Helicobacter species isolated from dolphins and whales.\" 4536 (2002a)","DOI":"10.1128\/JCM.40.12.4536-4543.2002"},{"key":"15","doi-asserted-by":"crossref","unstructured":"CM Harper, CA Dangler, S Xu, et al.  \"Appl Environ Microbiol.\"  66, \"Isolation and characterization of a Helicobacter sp. from the gastric mucosa of dolphins, Lagenorhynchus acutus and Delphinus delphis.\" 4751 (2000)","DOI":"10.1128\/AEM.66.11.4751-4757.2000"},{"key":"16","doi-asserted-by":"crossref","unstructured":"CMG Harper, S Xu, Y Feng, et al.  \"Appl Environ Microbiol.\"  68, \"Identification of novel Helicobacter spp. from a beluga whale.\" 2040 (2002b)","DOI":"10.1128\/AEM.68.4.2040-2043.2002"},{"key":"17","unstructured":"SA Hayes, E Josephson, K Maze-Foley, et al.  \"NOAA Technical Memorandum NMFS-NE-264.\"  \"US Atlantic and Gulf of Mexico marine mammal stock assessments\u20142019.\" (2020)"},{"key":"21","doi-asserted-by":"crossref","unstructured":"JG Kusters, AHM van Vliet, EJ Kuipers,  \"Clin Microbiol Rev.\"  19, \"Pathogenesis of Helicobacter pylori infection.\" 449 (2006)","DOI":"10.1128\/CMR.00054-05"},{"key":"22","doi-asserted-by":"crossref","unstructured":"M. Maiwald,  \"Molecular microbiology: Diagnostic principles and practice.\"  \"Broad-range PCR for detection and identification of bacteria.\" 491 (2011)","DOI":"10.1128\/9781555816834.ch31"},{"key":"24","doi-asserted-by":"crossref","unstructured":"B. Marshall,  \"Clin Med (Lond).\"  2, \"Helicobacter pylori: 20 years on.\" 147 (2002)","DOI":"10.7861\/clinmedicine.2-2-147"},{"key":"25","doi-asserted-by":"crossref","unstructured":"RW McLaughlin, JS Zheng, MM Chen, et al.  \"Dis Aquat Organ.\"  95, \"Detection of Helicobacter in the fecal material of the endangered Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis.\" 241 (2011)","DOI":"10.3354\/dao02364"},{"key":"26","doi-asserted-by":"crossref","unstructured":"I Mladenova-Hristova, O Grekova, A Patel,  \"J Microbiol Immunol Infect.\"  50, \"Zoonotic potential of Helicobacter spp.\" 265 (2017)","DOI":"10.1016\/j.jmii.2016.11.003"},{"key":"28","unstructured":"\"Marine mammal stranding report\u2014Level A (NOAA Form 89-864, OMB No. 0648\u20130178).\"  \"Examiner's guide, 2020 revision.\" (2020)"},{"key":"29","doi-asserted-by":"crossref","unstructured":"M Nei  and S Kumar  \"Molecular evolution and phylogenetics.\"  53 (2000)","DOI":"10.1093\/oso\/9780195135848.001.0001"},{"key":"30","doi-asserted-by":"publisher","unstructured":"P Onens, S Wilkin, D Fauquier, et al.  \"2019 report of marine mammal strandings in the United States: Southeast region.\"  (2023)","DOI":"10.25923\/m2zm-6n14"},{"key":"31","doi-asserted-by":"crossref","unstructured":"KR Pugliares, A Bogomolni, KM Touhey, et al.  \"Marine mammal necropsy: An introductory guide for stranding responders and field biologists.\"  165 (2007)","DOI":"10.1575\/1912\/1823"},{"key":"32","unstructured":"EA Ramos, API Luque, L Herrera, et al.  \"Lat Am J Aquat Mamm.\"  15, \"Stranding of a pregnant dwarf sperm whale (Kogia sima) in Utila, Honduras.\" 25 (2020)"},{"key":"33","unstructured":"RR Reeves, BS Stewart, PJ Clapham, et al.  \"Guide to marine mammals of the world.\"  528 (2002)"},{"key":"34","doi-asserted-by":"crossref","unstructured":"EW Sayers, M Cavanaugh, L Frisse, et al.  \"Nucleic Acids Res.\"  53, \"GenBank 2025 update.\" D56 (2025)","DOI":"10.1093\/nar\/gkae1114"},{"key":"35","doi-asserted-by":"crossref","unstructured":"T Schott, PK Kondadi, ML H\u00e4nninen, et al.  \"BMC Genomics.\"  12, \"Comparative genomics of Helicobacter pylori and the human-derived Helicobacter bizzozeronii CIII-1 strain reveal the molecular basis of the zoonotic nature of non-pylori gastric Helicobacter infections in humans.\" 534 (2011)","DOI":"10.1186\/1471-2164-12-534"},{"key":"36","doi-asserted-by":"crossref","unstructured":"T Segawa, Y Ohno, M Kurita, et al.  \"Dis Aquat Organ.\"  28, \"Helicobacter delphinicola infection and the risk of gastric disease in common bottlenose dolphin.\" 155 (2023)","DOI":"10.3354\/dao03751"},{"key":"37","doi-asserted-by":"crossref","unstructured":"AR. Sepulveda,  \"Curr Pathobiol Rep.\"  1, \"Helicobacter, inflammation, and gastric cancer.\" 9 (2013)","DOI":"10.1007\/s40139-013-0009-8"},{"key":"38","doi-asserted-by":"crossref","unstructured":"SI Shaaban, D Talat, SA Khatab, et al.  \"BMC Vet Res.\"  19, \"An investigative study on the zoonotic potential of Helicobacter pylori.\" 16 (2023)","DOI":"10.1186\/s12917-023-03572-w"},{"key":"39","doi-asserted-by":"crossref","unstructured":"A Smet, K Yahara, M Rossi, et al.  \"ISME J.\"  12, \"Macro-evolution of gastric Helicobacter species unveils inter-species admixture and time of divergence.\" 2518 (2018)","DOI":"10.1038\/s41396-018-0199-5"},{"key":"40","doi-asserted-by":"crossref","unstructured":"JV Solnick  and DB Schauer  \"Clin Microbiol Rev.\"  14, \"Emergence of diverse Helicobacter species in the pathogenesis of gastric and enterohepatic diseases.\" 59 (2001)","DOI":"10.1128\/CMR.14.1.59-97.2001"},{"key":"41","doi-asserted-by":"crossref","unstructured":"P Su\u00e1rez, M Contreras, M Fern\u00e1ndez-Delgado, et al.  \"J Wildl Dis.\"  46, \"Detection of Helicobacter in the digestive tract of an Atlantic spotted dolphin (Stenella frontalis).\" 622 (2010)","DOI":"10.7589\/0090-3558-46.2.622"},{"key":"44","doi-asserted-by":"crossref","unstructured":"KI Twing, DL Kirchman, BJ Campbell,  \"Water Res.\"  45, \"Temporal study of Helicobacter pylori presence in coastal freshwater, estuary and marine waters.\" 1897 (2011)","DOI":"10.1016\/j.watres.2010.12.013"},{"key":"45","doi-asserted-by":"crossref","unstructured":"AS Warthin  and AC Starry  \"J Inf Dis.\"  30, \"The staining of spirochetes in cover-glass smears by the silver-agar method.\" 592 (1922)","DOI":"10.1093\/infdis\/30.6.592"},{"key":"46","unstructured":"K. West,  \"Whales, dolphins, and porpoises: A natural history and species guide.\"  \"Dwarf sperm whale and pygmy sperm whale.\" 190 (2015)"},{"key":"43","doi-asserted-by":"crossref","unstructured":"K Tamura, G Stecher, S Kumar,  \"Mol Biol Evol.\"  38, \"MEGA11: Molecular Evolutionary Genetics Analysis Version 11.\" 3022 (2021)","DOI":"10.1093\/molbev\/msab120"},{"key":"23","doi-asserted-by":"crossref","unstructured":"CA Manire, HL Rhinehart, NB Barros, et al.  \"Aquat Mamm.\"  30, \"An approach to the rehabilitation of Kogia spp.\" 257 (2004)","DOI":"10.1578\/AM.30.2.2004.257"},{"key":"18","doi-asserted-by":"crossref","unstructured":"DM Hillis  and MT Dixon  \"Q Rev Biol.\"  66, \"Ribosomal DNA: molecular evolution and phylogenetic inference.\" 411 (1991)","DOI":"10.1086\/417338"},{"key":"20","doi-asserted-by":"crossref","unstructured":"M. Kimura,  \"J Mol Evol.\"  16, \"A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences.\" 111 (1980)","DOI":"10.1007\/BF01731581"},{"key":"19","doi-asserted-by":"crossref","unstructured":"K Katoh  and H Toh  \"BMC Bioinformatics.\"  9, \"Improved accuracy of multiple ncRNA alignment by incorporating structural information into a MAFFT-based framework.\" 212 (2008)","DOI":"10.1186\/1471-2105-9-212"},{"key":"42","doi-asserted-by":"crossref","unstructured":"JC Sweeney  and SH Ridgway  \"J Am Vet Med Assoc.\"  167, \"Common diseases of small cetaceans.\" 533 (1975)","DOI":"10.2460\/javma.1975.167.07.533"},{"key":"27","unstructured":"\"Basic local alignment search tool.\"  (2020)"}],"container-title":["Journal of Wildlife Diseases"],"original-title":[],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:18:55Z","timestamp":1791235135000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/journal-of-wildlife-diseases\/volume-62\/issue-1\/JWD-D-24-00204\/NOVEL-GASTRIC-HELICOBACTER-SPECIES-IN-STRANDED-PYGMY-SPERM-WHALES-KOGIA\/10.7589\/JWD-D-24-00204.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,16]]},"references-count":46,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,1,1]]}},"URL":"https:\/\/doi.org\/10.7589\/jwd-d-24-00204","relation":{},"ISSN":["0090-3558","1943-3700"],"issn-type":[{"value":"0090-3558","type":"print"},{"value":"1943-3700","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,16]]}}}