{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:56:54Z","timestamp":1791237414108,"version":"4.1.0"},"reference-count":14,"publisher":"Wildlife Disease Association","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Wildlife Diseases"],"published-print":{"date-parts":[[2026,2,16]]},"abstract":"<jats:p>Deciphering the biological and ecological drivers of virus transmission in wildlife requires specific molecular diagnostic tools. Understanding viral shedding routes, loads, and particle infectivity is crucial for assessing transmission dynamics in reservoir hosts and spillover potential to other species, including humans. We investigated coronavirus (CoV) shedding patterns in the endemic Molossid bat species Mormopterus francoismoutoui on Reunion Island. We tested 1,326 fresh feces with two PCR assays: a pan-CoV multiprobe real-time PCR and a quantitative PCR (qPCR) targeting the RdRp gene of the alpha-CoV previously described in M. francoismoutoui. Both assays showed strong agreement, although the qPCR detected 1.55 times more positives. Temporal variation in CoV shedding prevalence was consistent across assays, and no significant differences in RdRp gene copy numbers were observed between adult females and juveniles. Urbanization may increase Molossid bats interactions with humans, livestock, and synanthropic species; a comprehensive assessment of spillover potential of Molossid-associated alpha-CoVs is therefore critical.<\/jats:p>","DOI":"10.7589\/jwd-d-25-00075","type":"journal-article","created":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T09:44:52Z","timestamp":1771235092000},"page":"220-224","source":"Crossref","is-referenced-by-count":0,"title":["Alpha-Coronavirus Shedding in the Reunion Free-Tailed Bat (Mormopterus francoismoutoui)"],"prefix":"10.7589","volume":"62","author":[{"given":"Ga\u00eblle","family":"Lef\u00e8vre","sequence":"first","affiliation":[{"name":"Universit\u00e9 de La R\u00e9union, Processus Infectieux en Milieu Insulaire Tropical, Inserm 1187, CNRS 9192, IRD 249, Sainte-Clotilde, La R\u00e9union, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magali","family":"Turpin","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de La R\u00e9union, Processus Infectieux en Milieu Insulaire Tropical, Inserm 1187, CNRS 9192, IRD 249, Sainte-Clotilde, La R\u00e9union, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Alice","family":"Simbi","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de La R\u00e9union, Processus Infectieux en Milieu Insulaire Tropical, Inserm 1187, CNRS 9192, IRD 249, Sainte-Clotilde, La R\u00e9union, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muriel","family":"Dietrich","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de La R\u00e9union, Processus Infectieux en Milieu Insulaire Tropical, Inserm 1187, CNRS 9192, IRD 249, Sainte-Clotilde, La R\u00e9union, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Lebarbenchon","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de La R\u00e9union, Processus Infectieux en Milieu Insulaire Tropical, Inserm 1187, CNRS 9192, IRD 249, Sainte-Clotilde, La R\u00e9union, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4415","reference":[{"key":"11","doi-asserted-by":"crossref","unstructured":"C Lebarbenchon, B Ramasindrazana, L Joffrin, et al.  \"Emerg Microbes Infect.\"  6, \"Astroviruses in bats, Madagascar.\" e58 (2017)","DOI":"10.1038\/emi.2017.47"},{"key":"1","doi-asserted-by":"crossref","unstructured":"S Aguillon, G Le Minter, C Lebarbenchon, et al.  \"Ecol Evol.\"  13, \"A population in perpetual motion: Highly dynamic roosting behavior of a tropical island endemic bat.\" e9814 (2023)","DOI":"10.1002\/ece3.9814"},{"key":"2","doi-asserted-by":"crossref","unstructured":"BR Amman, SA Carroll, ZD Reed, et al.  \"PLoS Pathog.\"  8, \"Seasonal pulses of Marburg virus circulation in juvenile Rousettus aegyptiacus bats coincide with periods of increased risk of human infection.\" e1002877 (2012)","DOI":"10.1371\/journal.ppat.1002877"},{"key":"3","doi-asserted-by":"crossref","unstructured":"DJ Becker, GF Albery, MK Kessler, et al.  \"J Anim Ecol.\"  89, \"Macro-immunology: The drivers and consequences of spatial patterns in wildlife immune defence.\" 972 (2020)","DOI":"10.1111\/1365-2656.13166"},{"key":"4","doi-asserted-by":"crossref","unstructured":"M Dietrich, DA Wilkinson, A Benlali, et al.  \"Environ Microbiol.\"  17, \"Leptospira and paramyxovirus infection dynamics in a bat maternity enlightens pathogen maintenance in wildlife.\" 4280 (2015)","DOI":"10.1111\/1462-2920.12766"},{"key":"5","doi-asserted-by":"crossref","unstructured":"SM Goodman, B Jansen van Vuuren, F Ratrimomanarivo, et al.  \"J Mammal.\"  89, \"Specific status of populations in the Mascarene Islands referred to Mormopterus acetabulosus (Chiroptera: Molossidae), with description of a new species.\" 1316 (2008)","DOI":"10.1644\/07-MAMM-A-232.1"},{"key":"6","unstructured":"L. H\u00e9r\u00e9,  \"Contribution \u00e0 l'\u00e9tude des chiropt\u00e8res de l'\u00eele de La R\u00e9union: R\u00e9partition et habitats prioritaires en mati\u00e8re de conservation.\"  39 (2009)"},{"key":"7","doi-asserted-by":"crossref","unstructured":"AOG Hoarau, M K\u00f6ster, M Dietrich, et al.  \"Epidemiol Infect.\"  151, \"Synchronicity of viral shedding in molossid bat maternity colonies.\" e47 (2023)","DOI":"10.1017\/S0950268823000171"},{"key":"8","doi-asserted-by":"crossref","unstructured":"L Joffrin, SM Goodman, DA Wilkinson, et al.  \"Sci Rep.\"  10, \"Bat coronavirus phylogeography in the Western Indian Ocean.\" 6873 (2020)","DOI":"10.1038\/s41598-020-63799-7"},{"key":"9","doi-asserted-by":"crossref","unstructured":"L Joffrin, AOG Hoarau, E Lagadec, et al.  \"R Soc Open Sci.\"  9, \"Seasonality of coronavirus shedding in tropical bats.\" 211600 (2022)","DOI":"10.1098\/rsos.211600"},{"key":"10","doi-asserted-by":"crossref","unstructured":"K Jung  and CG Threlfall  \"Bats in the Anthropocene: Conservation of bats in a changing world.\"  \"Urbanisation and its effects on bats\u2014Global meta-analysis.\" 13 (2016)","DOI":"10.1007\/978-3-319-25220-9_2"},{"key":"12","doi-asserted-by":"crossref","unstructured":"S Muradrasoli, N Mohamed, A Horny\u00e1k, et al.  \"J Virol Methods.\"  159, \"Broadly targeted multiprobe q-PCR for detection of coronaviruses: Coronavirus is common among mallard ducks (Anas platyrhynchos).\" 277 (2009)","DOI":"10.1016\/j.jviromet.2009.04.022"},{"key":"13","doi-asserted-by":"crossref","unstructured":"RK Plowright, P Eby, PJ Hudson, et al.  \"Proc R Soc B.\"  282, \"Ecological dynamics of emerging bat virus spillover.\" 20142124 (2015)","DOI":"10.1098\/rspb.2014.2124"},{"key":"14","doi-asserted-by":"crossref","unstructured":"RV Ramanantsalama, DA Wilkinson, RL Muylaert, et al.  \"One Health.\"  21, \"Predicting current and future distributions of Malagasy bats: Implications for management of coronavirus spillover.\" 101122 (2025)","DOI":"10.1016\/j.onehlt.2025.101122"}],"container-title":["Journal of Wildlife Diseases"],"original-title":[],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:19:23Z","timestamp":1791235163000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/journal-of-wildlife-diseases\/volume-62\/issue-1\/JWD-D-25-00075\/Alpha-Coronavirus-Shedding-in-the-Reunion-Free-Tailed-Bat-Mormopterus\/10.7589\/JWD-D-25-00075.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,16]]},"references-count":14,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,1,1]]}},"URL":"https:\/\/doi.org\/10.7589\/jwd-d-25-00075","relation":{},"ISSN":["0090-3558","1943-3700"],"issn-type":[{"value":"0090-3558","type":"print"},{"value":"1943-3700","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,16]]}}}