{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T23:29:19Z","timestamp":1791588559449,"version":"4.3.4"},"reference-count":38,"publisher":"Wildlife Disease Association","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Wildlife Diseases"],"published-print":{"date-parts":[[2026,4,20]]},"abstract":"<jats:p>Barren-ground caribou of the subspecies Rangifer tarandus granti are distributed throughout Alaska, USA, and the Yukon, Canada. The acute phase response is an innate immune response activated due to inflammatory stimulus, resulting in changing levels of acute phase proteins, including haptoglobin (Hp) and serum amyloid A (SAA). Haptoglobin and SAA are used as indicators of inflammation in domestic species, with several studies demonstrating their use in wildlife. In this study, banked serum samples from apparently clinically normal free-ranging barren-ground caribou (reference group) were used to establish reference intervals (RI) for Hp and SAA in caribou. These RIs were compared with additional samples from a declining herd with ongoing Brucella suis biovar 4 transmission. The RIs for Hp and SAA with 90% upper confidence intervals were 0.12\u20131.03 (0.08\u20131.27) mg\/mL and 0.10\u201318.44 (0.10\u201331.00) mg\/L, respectively. The SAA levels in the declining herd were found to be significantly elevated from the reference group (P=0.009), whereas no difference was found in Hp levels between the two herds (P=0.389). Meanwhile, SAA and Hp levels in animals with B. suis biovar 4 titers \u22651:80 were significantly elevated from the reference group (SAA P&lt;0.001; Hp P=0.178). The results of our study indicate that SAA and Hp hold promise in monitoring the overall herd health of caribou in Alaska.<\/jats:p>","DOI":"10.7589\/jwd-d-25-00078","type":"journal-article","created":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T05:51:37Z","timestamp":1776664297000},"page":"349-359","source":"Crossref","is-referenced-by-count":1,"title":["ESTABLISHMENT OF REFERENCE INTERVALS FOR SERUM AMYLOID A AND HAPTOGLOBIN IN FREE-RANGING BARREN-GROUND CARIBOU (RANGIFER TARANDUS GRANTI), AND HERD HEALTH IMPLICATIONS"],"prefix":"10.7589","volume":"62","author":[{"given":"Emma R.","family":"Rovani-Rhoades","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, 110 Ott Road, Pullman, Washington 99164, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig S.","family":"McConnel","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, 110 Ott Road, Pullman, Washington 99164, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberlee B.","family":"Beckmen","sequence":"additional","affiliation":[{"name":"Alaska Department of Fish and Game, Division of Wildlife Conservation, 1300 College Road, Fairbanks, Alaska 99701, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn","family":"Cray","sequence":"additional","affiliation":[{"name":"Division of Comparative Pathology, Department of Pathology & Laboratory Medicine, University of Miami Miller School of Medicine, PO Box 016960 R-46, Miami, Florida 33101, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindsey M.","family":"Dreese","sequence":"additional","affiliation":[{"name":"Alaska Department of Fish and Game, Division of Wildlife Conservation, 1300 College Road, Fairbanks, Alaska 99701, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4415","reference":[{"key":"22","unstructured":"\"Microsoft Excel.\"  (2025)"},{"key":"1","unstructured":"XF Aguilar  and S Kutz  \"Dolphin and Union caribou health assessment.\"  (2020)"},{"key":"4","unstructured":"L. Castrodale,  \"Human and animal brucellosis in Alaska.\"  (2011)"},{"key":"6","doi-asserted-by":"crossref","unstructured":"LK Duffy, RT Bowyer, JW Testa, et al.  \"J Wildl Dis.\"  29, \"Differences in blood haptoglobin and length\u2013mass relationships in river otters (Lutra canadensis) from oiled and nonoiled areas of Prince William Sound, Alaska.\" 353 (1993)","DOI":"10.7589\/0090-3558-29.2.353"},{"key":"8","unstructured":"LB. Forbes,  \"Can Vet J.\"  32, \"Isolates of Brucella suis biovar 4 from animals and humans in Canada, 1982\u20131990.\" 686 (1991)"},{"key":"12","doi-asserted-by":"crossref","unstructured":"SN Grell, K Tj\u00f8rneh\u00f8j, LE Larsen, et al.  \"Vet Immunol Immunopathol.\"  103, \"Marked induction of IL-6, haptoglobin and IFN\u03b3 following experimental BRSV infection in young calves.\" 235 (2005)","DOI":"10.1016\/j.vetimm.2004.09.025"},{"key":"13","doi-asserted-by":"crossref","unstructured":"PMH Heegaard, DL Godson, MJM Toussaint, et al.  \"Vet Immunol Immunopathol.\"  77, \"The acute phase response of haptoglobin and serum amyloid A (SAA) in cattle undergoing experimental infection with bovine respiratory syncytial virus.\" 151 (2000)","DOI":"10.1016\/S0165-2427(00)00226-9"},{"key":"14","doi-asserted-by":"crossref","unstructured":"EH Hooijberg  and C Cray  \"Vet Clin Pathol.\"  52, \"Acute phase reactants in non-domesticated mammals\u2014A veterinary clinical pathology perspective.\" 19 (2023)","DOI":"10.1111\/vcp.13189"},{"key":"16","doi-asserted-by":"crossref","unstructured":"A Kakuschke, HB Erbsloeh, S Griesel, et al.  \"Comp Biochem Physiol B Biochem Mol Biol.\"  155, \"Acute phase protein haptoglobin in blood plasma samples of harbour seals (Phoca vitulina) of the Wad-den Sea and of the isle Helgoland.\" 67 (2010)","DOI":"10.1016\/j.cbpb.2009.10.002"},{"key":"17","doi-asserted-by":"crossref","unstructured":"SN Kennedy, JM Castellini, AB Hayden, et al.  \"J Wildl Dis.\"  55, \"Regional and age-related variations in haptoglobin concentrations in stellar sea lions (Eumetopias jubatus) from Alaska, USA.\" 91 (2019)","DOI":"10.7589\/2017-10-257"},{"key":"18","doi-asserted-by":"crossref","unstructured":"AKH Krogh, JFH Lundsgaard, J Bakker, et al.  \"J Zoo Wildl Med.\"  45, \"Acute-phase responses in healthy and diseased rhesus macaques (Macaca mulatta).\" 306 (2014)","DOI":"10.1638\/2013-0153R.1"},{"key":"19","doi-asserted-by":"crossref","unstructured":"I Kushner  and A Mackiewicz  \"Acute phase proteins: Molecular biology, biochemistry, and clinical applications.\"  \"The acute phase response: An overview.\" 3 (2020)","DOI":"10.1201\/9781003068587-2"},{"key":"20","doi-asserted-by":"crossref","unstructured":"AP Levy, R Asleh, S Blum, et al.  \"Antioxid Redox Signal.\"  12, \"Haptoglobin: Basic and clinical aspects.\" 293 (2010)","DOI":"10.1089\/ars.2009.2793"},{"key":"21","unstructured":"\"XLSTAT statistical and data analysis solution.\"  (2023)"},{"key":"23","doi-asserted-by":"crossref","unstructured":"KA Neiland, JA King, BE Huntley, et al.  \"Wildl Dis.\"  4, \"The diseases and parasites of Alaskan wildlife populations, part I: Some observations on brucellosis in caribou.\" 27 (1968)","DOI":"10.7589\/0090-3558-4.2.27"},{"key":"24","doi-asserted-by":"crossref","unstructured":"IH Nymo, K Beckmen, J Godfroid,  \"J Wildl Dis.\"  52, \"Anti-brucella antibodies in moose (Alces alces gigas), muskoxen (Ovibos moschatus), and plains bison (Bison bison bison) in Alaska, USA.\" 96 (2016)","DOI":"10.7589\/2015-04-100"},{"key":"25","unstructured":"SE. Oderkirk,  \"Clinical pathology of intensively managed reindeer.\"  52 (1998)"},{"key":"26","doi-asserted-by":"crossref","unstructured":"T Orro, T Pohjanvirta, U Rikula, et al.  \"Comp Immunol Microbiol Infect Dis.\"  34, \"Acute phase protein changes in calves during an outbreak of respiratory disease caused by bovine respiratory syncytial virus.\" 23 (2011)","DOI":"10.1016\/j.cimid.2009.10.005"},{"key":"28","doi-asserted-by":"crossref","unstructured":"J Pastor, E Bach, A R\u00e1ez-Bravo, et al.  \"Vet Clin Pathol.\"  48, \"Method validation, reference values, and characterization of acute-phase protein responses to experimentally induced inflammation and bluetongue virus infection in the Iberian ibex.\" 695 (2019)","DOI":"10.1111\/vcp.12802"},{"key":"31","doi-asserted-by":"crossref","unstructured":"H Sharifiyazdia, S Nazifi, K Nikseresht, et al.  \"J Bacteriol Parasitol.\"  3, \"Evaluation of serum amyloid A and haptoglobin in dairy cows naturally infected with brucellosis.\" 1000157 (2012)","DOI":"10.4172\/2155-9597.1000157"},{"key":"32","unstructured":"RO. Skoog,  \"Ecology of the caribou (Rangifer tarandus granti) in Alaska.\"  720 (1968)"},{"key":"34","doi-asserted-by":"crossref","unstructured":"M Tryland  and SJ Kutz  \"Reindeer and caribou: Health and disease.\"  \"Caribou management: An overview from northern Canada and Alaska.\" 43 (2019)","DOI":"10.1201\/9780429489617"},{"key":"35","doi-asserted-by":"crossref","unstructured":"P. Valkenburg,  \"Rangifer.\"  18, \"Herd size, distribution, harvest, management issues, and research priorities relevant to caribou herds in Alaska.\" 125 (1998)","DOI":"10.7557\/2.18.5.1549"},{"key":"36","doi-asserted-by":"crossref","unstructured":"J Vicente, J Martinez-Guijosa, A Tvarijonaviciute, et al.  \"Comp Immunol Microbiol Infect Dis.\"  64, \"Serum haptoglobin response in red deer naturally infected with tuberculosis.\" 25 (2019)","DOI":"10.1016\/j.cimid.2019.01.021"},{"key":"37","doi-asserted-by":"crossref","unstructured":"B Wells, GT Innocent, PD Eckersall, et al.  \"Vet Res.\"  44, \"Two major ruminant acute phase proteins, haptoglobin and serum amyloid A, as serum biomarkers during active sheep scab infestation.\" 103 (2013)","DOI":"10.1186\/1297-9716-44-103"},{"key":"33","doi-asserted-by":"crossref","unstructured":"K Tobin, D Zimmerman, J Rasmussen, et al.  \"J Zoo Wildl Med.\"  51, \"Establishment of acute-phase protein and serum protein electrophoresis preliminary reference values for pronghorn (Antilocapra americana).\" 321 (2020)","DOI":"10.1638\/2018-0226"},{"key":"5","unstructured":"C Cray, J Zaias, NH Altman,  \"Comp Med.\"  59, \"Acute phase response in animals: a review.\" 517 (2009)"},{"key":"9","doi-asserted-by":"publisher","unstructured":"KR Friedrichs, KE Harr, KP Freeman, et al.  \"Vet Clin Pathol.\"  41, \"ASVCP reference interval guidelines: determination of de novo reference intervals in veterinary species and other related topics.\" 441 (2012)","DOI":"10.1111\/vcp.12006"},{"key":"2","doi-asserted-by":"crossref","unstructured":"MF Bertelsen, M Kjelgaard-Hansen, C Gr\u00f8ndahl, et al.  \"J Zoo Wildl Med.\"  40, \"Identification of acute phase proteins and assays applicable in nondomesticated mammals.\" 199 (2009)","DOI":"10.1638\/2007-0125.1"},{"key":"38","doi-asserted-by":"crossref","unstructured":"T Zenteno-Savin, MA Castellini, LD Rea, et al.  \"J Wildl Dis.\"  33, \"Plasma haptoglobin levels in threatened Alaskan pinniped populations.\" 64 (1997)","DOI":"10.7589\/0090-3558-33.1.64"},{"key":"29","doi-asserted-by":"publisher","unstructured":"HH Petersen, JP Nielsen, PMH Heegaard,  \"Vet Res.\"  35, \"Application of acute phase protein measurements in veterinary clinical chemistry.\" 163 (2004)","DOI":"10.1051\/vetres:2004002"},{"key":"11","doi-asserted-by":"crossref","unstructured":"J Godfroid, B Garin-Bastuji, C Saegerman, et al.  \"Rev Sci Tech.\"  32, \"Brucellosis in terrestrial wildlife.\" 27 (2013)","DOI":"10.20506\/rst.32.1.2180"},{"key":"15","doi-asserted-by":"publisher","unstructured":"R Isaza, E Wiedner, S Hiser, et al.  \"J Vet Diagn Invest.\"  26, \"Reference intervals for acute phase protein and serum protein electrophoresis values in captive Asian elephants (Elephas maximus).\" 616 (2014)","DOI":"10.1177\/1040638714543923"},{"key":"10","doi-asserted-by":"crossref","unstructured":"A Geffr\u00e9, D Concordet, JP Braun, et al.  \"Vet Clin Pathol.\"  40, \"Reference Value Advisor: a new freeware set of macroinstructions to calculate reference intervals with Microsoft Excel.\" 107 (2011)","DOI":"10.1111\/j.1939-165X.2011.00287.x"},{"key":"27","doi-asserted-by":"crossref","unstructured":"T Orro, S Sankari, T Pudas, et al.  \"Comp Immunol Microbiol Infect Dis.\"  27, \"Acute phase response in reindeer after challenge with Escherichia coli endotoxin.\" 413 (2004)","DOI":"10.1016\/j.cimid.2004.01.005"},{"key":"7","doi-asserted-by":"crossref","unstructured":"PD. Eckersall,  \"Clinical biochemistry of domestic animals.\"  \"Proteins, proteomics, and the dysproteinemias.\" 117 (2008)","DOI":"10.1016\/B978-0-12-370491-7.00005-2"},{"key":"3","doi-asserted-by":"crossref","unstructured":"KJ Bondo, B Macbeth, H Schwantje, et al.  \"J Wildl Dis.\"  55, \"Health survey of boreal caribou (Rangifer tarandus caribou) in northeastern British Columbia, Canada.\" 544 (2019)","DOI":"10.7589\/2018-01-018"},{"key":"30","unstructured":"\"R: A Language and Environment for Statistical Computing.\"  (2023)"}],"container-title":["Journal of Wildlife Diseases"],"original-title":[],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:02:37Z","timestamp":1791234157000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/journal-of-wildlife-diseases\/volume-62\/issue-2\/JWD-D-25-00078\/ESTABLISHMENT-OF-REFERENCE-INTERVALS-FOR-SERUM-AMYLOID-A-AND-HAPTOGLOBIN\/10.7589\/JWD-D-25-00078.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,20]]},"references-count":38,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,4,1]]}},"URL":"https:\/\/doi.org\/10.7589\/jwd-d-25-00078","relation":{},"ISSN":["0090-3558","1943-3700"],"issn-type":[{"value":"0090-3558","type":"print"},{"value":"1943-3700","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,20]]}}}