{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:53:48Z","timestamp":1791237228464,"version":"4.1.0"},"reference-count":24,"publisher":"Wildlife Disease Association","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Wildlife Diseases"],"published-print":{"date-parts":[[2026,4,20]]},"abstract":"<jats:p>Flaviviruses (genus Orthoflavivirus, family Flaviviridae) are positive-sense RNA viruses able to infect a wide range of hosts and are associated with disease in humans, domestic animals and wildlife; thus, they are considered a major global health concern. The sylvatic cycle is potentially linked with human outbreaks; therefore, it is essential to monitor and understand flavivirus infections in wildlife. Little is known about flaviviruses in cetaceans. To fill part of this knowledge gap, we surveyed for the presence of flavivirus RNA in tissues (brain, spleen, lung, kidney, lymph node, liver, and adrenal gland) and serum samples of 151 cetaceans of 22 species that were found stranded, bycaught, or live captured in scientific expeditions in Brazil in two different biomes (marine and Amazon basin). Total RNA was extracted from 589 samples (523 tissues and 66 sera) using commercial kits; subsequently, a broad-range real-time PCR targeting the Orthoflavivirus genus was performed. All individuals were negative for flavivirus RNA (95% confidence interval, 0\u20131.96%). These results potentially indicate that the known flaviviruses, if present, have limited circulation in wild cetaceans, likely accidental hosts. Future studies are warranted to understand the epidemiology of flaviviruses within the genus Orthoflavivirus in cetaceans.<\/jats:p>","DOI":"10.7589\/jwd-d-25-00149","type":"journal-article","created":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T05:57:27Z","timestamp":1776664647000},"page":"482-488","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Survey for Flaviviruses in Wild Cetaceans from Brazil"],"prefix":"10.7589","volume":"62","author":[{"given":"Aricia","family":"Duarte-Benvenuto","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, Rua Prof. Dr. Orlando Marques de Paiva 87, S\u00e3o Paulo, 05508-270, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Sacrist\u00e1n","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal, Consejo Superior de Investigaciones Cient\u00edficas (CSIC), Carretera Algete-El Casar de Talamanca, Km. 8,1, Valdeolmos, 28130, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana C.","family":"Ewbank","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal, Consejo Superior de Investigaciones Cient\u00edficas (CSIC), Carretera Algete-El Casar de Talamanca, Km. 8,1, Valdeolmos, 28130, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samira","family":"Costa-Silva","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, Rua Prof. Dr. Orlando Marques de Paiva 87, S\u00e3o Paulo, 05508-270, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K\u00e1tia R.","family":"Groch","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine & Biomedical Sciences, Texas A&M University, 664 Raymond Stotzer Parkway, College Station, Texas 77843, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josu\u00e9","family":"D\u00edaz-Delgado","sequence":"additional","affiliation":[{"name":"Texas A&M Veterinary Medical Diagnostic Laboratory, 483 Agronomy Road, College Station, Texas 77843, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Ib\u00e1\u00f1ez-Porras","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Sanidad Animal, Consejo Superior de Investigaciones Cient\u00edficas (CSIC), Carretera Algete-El Casar de Talamanca, Km. 8,1, Valdeolmos, 28130, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana Castaldo","family":"Colosio","sequence":"additional","affiliation":[{"name":"Instituto Baleia Jubarte, Rua Bar\u00e3o do Rio Branco, 125, Centro, Caravelas, 45900-000, Bah\u00eda, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hernani da","family":"C. G. Ramos","sequence":"additional","affiliation":[{"name":"Instituto Baleia Jubarte, Rua Bar\u00e3o do Rio Branco, 125, Centro, Caravelas, 45900-000, Bah\u00eda, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vitor L.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Associa\u00e7\u00e3o para Pesquisa e Preserva\u00e7\u00e3o de Ecossistemas Aqu\u00e1ticos, Avenida Pintor Jo\u00e3o Figueiredo, Servi\u00e7o Social de Comercio (SESC), Iparana, Caucaia, 61627-250, Cear\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa L.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Biopesca Institute, Rua Carlos Eduardo Conte de Castro, 93 Canto do Forte, Praia Grande, S\u00e3o Paulo, 11700-430, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina Pacheco","family":"Bertozzi","sequence":"additional","affiliation":[{"name":"Biopesca Institute, Rua Carlos Eduardo Conte de Castro, 93 Canto do Forte, Praia Grande, S\u00e3o Paulo, 11700-430, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline Freitas","family":"Pessi","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, Rua Prof. Dr. Orlando Marques de Paiva 87, S\u00e3o Paulo, 05508-270, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waleska","family":"Gravena","sequence":"additional","affiliation":[{"name":"Instituto de Sa\u00fade e Biotecnologia, Universidade Federal do Amazonas, Estrada Coari\/Mami\u00e1, 305, Bairro: Esp\u00edrito Santo, Coari, 69460-000, Amazonas, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera M.","family":"Ferreira da Silva","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Pesquisas da Amaz\u00f4nia, Av. Andr\u00e9 Ara\u00fajo, 2936, Manaus, 69067-375, Amazonas, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Marmontel","sequence":"additional","affiliation":[{"name":"Instituto de Desenvolvimento Sustent\u00e1vel Mamirau\u00e1, Fonte Boa, Estr. do Bexiga, 2584, Tef\u00e9, 69553-225, Amazonas, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana","family":"Marigo","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, Rua Prof. Dr. Orlando Marques de Paiva 87, S\u00e3o Paulo, 05508-270, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 L.","family":"Cat\u00e3o-Dias","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, Rua Prof. Dr. Orlando Marques de Paiva 87, S\u00e3o Paulo, 05508-270, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4415","reference":[{"key":"1","doi-asserted-by":"crossref","unstructured":"MR Blahove  and JR Carter  \"Viruses.\"  13, \"Flavivirus persistence in wildlife populations.\" 2099 (2021)","DOI":"10.3390\/v13102099"},{"key":"3","doi-asserted-by":"crossref","unstructured":"C Buck, GP Paulino, DJ Medina, et al.  \"Clin Diagn Virol.\"  1, \"Isolation of St. Louis encephalitis virus from a killer whale.\" 109 (1993)","DOI":"10.1016\/0928-0197(93)90018-Z"},{"key":"4","doi-asserted-by":"crossref","unstructured":"RN. 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Bossart,  \"Vet Pathol.\"  48, \"Marine mammals as sentinel species for oceans and human health.\" 676 (2011)","DOI":"10.1177\/0300985810388525"},{"key":"24","doi-asserted-by":"crossref","unstructured":"SC Weaver  and AD Barrett  \"Nat Rev Microbiol.\"  2, \"Transmission cycles, host range, evolution and emergence of arboviral disease.\" 789 (2004)","DOI":"10.1038\/nrmicro1006"},{"key":"16","doi-asserted-by":"crossref","unstructured":"G Moureau, S Temmam, JP Gonzalez, et al.  \"Vector Borne Zoonotic Dis.\"  7, \"A real-time RT-PCR method for the universal detection and identification of flaviviruses.\" 467 (2007)","DOI":"10.1089\/vbz.2007.0206"},{"key":"20","doi-asserted-by":"crossref","unstructured":"P Simmonds, P Becher, J Bukh, et al.  \"J Gen Virol.\"  98, \"ICTV virus taxonomy profile: Flaviviridae.\" 2 (2017)","DOI":"10.1099\/jgv.0.000672"},{"key":"21","doi-asserted-by":"crossref","unstructured":"E Simulundu, K Ndashe, HM Chambaro, et al.  \"Emerg Infect Dis.\"  26, \"West Nile virus in farmed crocodiles, Zambia, 2019.\" 811 (2020)","DOI":"10.3201\/eid2604.190954"}],"container-title":["Journal of Wildlife Diseases"],"original-title":[],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:03:01Z","timestamp":1791234181000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/journal-of-wildlife-diseases\/volume-62\/issue-2\/JWD-D-25-00149\/Molecular-Survey-for-Flaviviruses-in-Wild-Cetaceans-from-Brazil\/10.7589\/JWD-D-25-00149.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,20]]},"references-count":24,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,4,1]]}},"URL":"https:\/\/doi.org\/10.7589\/jwd-d-25-00149","relation":{},"ISSN":["0090-3558","1943-3700"],"issn-type":[{"value":"0090-3558","type":"print"},{"value":"1943-3700","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,20]]}}}