{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:30:47Z","timestamp":1759332647566,"version":"3.37.3"},"reference-count":23,"publisher":"Instituto Nacional de Salud (Colombia)","issue":"1","content-domain":{"domain":["revistabiomedica.org"],"crossmark-restriction":false},"short-container-title":["biomedica"],"abstract":"<jats:p>Introducci\u00f3n. Dadas las dificultades del diagn\u00f3stico microsc\u00f3pico de la malaria o paludismo en las \u00e1reas rurales, las pruebas de diagn\u00f3stico r\u00e1pido constituyen una buena alternativa, por lo que es importante conocer su desempe\u00f1o.Objetivo. Evaluar el desempe\u00f1o de las pruebas de diagn\u00f3stico r\u00e1pido utilizadas en cinco departamentos para al diagn\u00f3stico microsc\u00f3pico de la malaria usando la reacci\u00f3n en cadena de la polimerasa (PCR) como est\u00e1ndar de referencia.Materiales y m\u00e9todos. Se usaron la prueba de gota gruesa y las pruebas de diagn\u00f3stico r\u00e1pido y, adem\u00e1s, se impregn\u00f3 papel de filtro con sangre para la prueba molecular (PCR), en individuos sintom\u00e1ticos.Resultados. Se incluyeron 314 muestras cuyo porcentaje de positividad para malaria fue de 49 % con la PCR, de 48 % con microscop\u00eda y de 46 % con las pruebas de diagn\u00f3stico r\u00e1pido; la parasitemia fluctu\u00f3 entre 180 y 23.800 par\u00e1sitos\/\u03bcl de sangre. La concordancia de los resultados de los puestos de microscop\u00eda comparados con la PCR (Laboratorio Nacional de Referencia) fueron los siguientes: coeficiente kappa de Cohen de 0,975 (IC95% 0,950-0,999), sensibilidad de 97 % (IC95% 95-100) y especificidad de 100 % (IC95% 100-100), e \u00edndice kappa de especie de 0,958 (IC95% 0,912-1,00). La concordancia de los resultados de la prueba de diagn\u00f3stico r\u00e1pido Pf\/Pv en los puestos de microscop\u00eda y los de la PCR (Laboratorio Nacional de Referencia), fue la siguiente: coeficiente kappa de 0,878 (IC95% 0,784-0,973), sensibilidad de 94 % (IC95% 87-100), especificidad de 95 % (IC95% 90-100), e \u00edndice kappa de especie de 1,0 (IC95% 1,00-1,00). La concordancia entre la prueba de diagn\u00f3stico r\u00e1pido Pf\/Pan y la PCR fue la siguiente: coeficiente kappa de Cohen de 0,920 (IC95% 0,865-0,974), sensibilidad de 94 % (IC95% 90-98), especificidad de 99 % (IC95% 95-100), e \u00edndice kappa de especie de 0,750 (IC95% 0,637-0,863).Conclusi\u00f3n. Los resultados de este estudio respaldan el uso de las pruebas de diagn\u00f3stico r\u00e1pido en Colombia, aunque se requiere un mejor entrenamiento del personal para diferenciar eficientemente las especies de Plasmodium.<\/jats:p>","DOI":"10.7705\/biomedica.4893","type":"journal-article","created":{"date-parts":[[2020,3,19]],"date-time":"2020-03-19T15:56:37Z","timestamp":1584633397000},"page":"117-128","update-policy":"https:\/\/doi.org\/10.7705\/crossmark","source":"Crossref","is-referenced-by-count":4,"title":["An\u00e1lisis de concordancia de tres pruebas para el diagn\u00f3stico de malaria en la poblaci\u00f3n sintom\u00e1tica de los municipios end\u00e9micos de Colombia"],"prefix":"10.7705","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1727-7335","authenticated-orcid":false,"given":"Liliana Jazm\u00edn","family":"Cort\u00e9s","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6133-8502","authenticated-orcid":false,"given":"\u00c1ngela Patricia","family":"Guerra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4444","published-online":{"date-parts":[[2020,3,1]]},"reference":[{"key":"ref0","unstructured":"Organizaci\u00f3n Panamericana de la Salud-Organizaci\u00f3n Mundial de la Salud. 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